A role for calcium in Bcl-2 action?


:Changes in the cytosolic Ca(2+) concentration ([Ca(2+)](c)) translate a variety of extracellular signals into widely diverse intracellular effects, ranging from secretion to movement, proliferation and also cell death. As regards the last one, it has long been known that large [Ca(2+)](c) increases lead cells to death. More recently, experimental evidence has been obtained that the oncogene Bcl-2 reduces the state of filling of intracellular Ca(2+) stores and thus affects the Ca(2+) responses induced by physiological and pathological stimuli. In this contribution, we will discuss this effect and its significance for the mechanism of action of Bcl-2, an important checkpoint of the apoptotic process.






Pinton P,Ferrari D,Rapizzi E,Di Virgilio F,Pozzan T,Rizzuto R





Has Abstract


2002-02-01 00:00:00














  • A multi-functional enzyme with RNA polymerase and RNase activities: molecular anatomy of influenza virus RNA polymerase.

    abstract::Influenza virus-associated RNA polymerase is composed of one molecule each of three viral P proteins and carries the complete activity of capped RNA-primed vRNA-directed transcription. The RNA polymerase holoenzyme also carries capped RNA endonuclease to generate capped oligonucleotide primers for transcription and 3'...


    pub_type: 杂志文章,评审


    authors: Ishihama A

    更新日期:1996-01-01 00:00:00

  • Biochemical characterization, stability studies and N-terminal sequence of a bi-functional inhibitor from Phaseolus aureus Roxb. (Mung bean).

    abstract::Herein, we report the purification and biochemical characterization of a novel bi-functional protein proteinase/amylase inhibitor from the dietary leguminous pulse Phaseolus aureus Roxb. (Vigna radiata L.) by means of acetic acid precipitation, salt fractionation, ion-exchange chromatography (DEAE-cellulose) and affin...


    pub_type: 杂志文章


    authors: Haq SK,Atif SM,Khan RH

    更新日期:2005-12-01 00:00:00

  • Characteristics of beta-adrenergic receptors in isolated cells and in crude membranes of brown adipose tissue.

    abstract::In relation to decreased metabolic sensitivity to catecholamines observed, in vitro, in brown fat of cold-acclimated rats, beta-adrenergic receptors were studied in isolated cells and in a crude membrane preparation from rat interscapular brown adipose tissue. [3H] dihydroalprenolol binding had the same characteristic...


    pub_type: 杂志文章


    authors: Senault C,Le Comte V,Portet R

    更新日期:1984-07-01 00:00:00

  • Identification and biochemical characterization of Rap2C, a new member of the Rap family of small GTP-binding proteins.

    abstract::The Rap family of small GTP-binding proteins is composed by four different members: Rap1A, Rap1B, Rap2A and Rap2B. In this work we report the identification and characterization of a fifth member of this family of small GTPases. This new protein is highly homologous to Rap2A and Rap2B, binds labeled GTP on nitrocellul...


    pub_type: 杂志文章


    authors: Paganini S,Guidetti GF,Catricalà S,Trionfini P,Panelli S,Balduini C,Torti M

    更新日期:2006-03-01 00:00:00

  • Loop residues of thrombin-binding DNA aptamer impact G-quadruplex stability and thrombin binding.

    abstract::The thrombin-binding DNA aptamer (TBA) 5'-d(GGTTGGTGTGGTTGG)-3' forms a G-quadruplex that is necessary for binding to the coagulation factor thrombin. The stability of the G-quadruplex of TBA when bound to thrombin and potassium ion (K(+)) were investigated for the wild-type oligonucleotide and for mutants in which th...


    pub_type: 杂志文章


    authors: Nagatoishi S,Isono N,Tsumoto K,Sugimoto N

    更新日期:2011-08-01 00:00:00

  • [Application of the theory of oxygen transfer: determination of the Michaelis constant of glucose oxidase with respect to oxygen].

    abstract::The oxidation of beta-D-glucose with glucose oxidase generally requires oxygen, which, under normal conditions is present at low concentrations in the reaction medium. Experiments show that glucose oxidase is no longer saturated by oxygen at enzyme concentrations greater than 0.4 mg.ml1. This is due to the decrease in...


    pub_type: 杂志文章


    authors: Boudrant J,Cheftel C

    更新日期:1975-01-01 00:00:00

  • Elongating ribosomes in vivo are refractory to erythromycin.

    abstract::We have studied the kinetics of erythromycin inhibition of translation in growing bacteria. In order to simplify the interpretation of our data, we have used a mutant (envA), known to have an increased permeability to several antibiotics, including erythromycin. The data clearly show that an initial stage of translati...


    pub_type: 杂志文章


    authors: Andersson S,Kurland CG

    更新日期:1987-08-01 00:00:00

  • Activation of the nuclear factor kappa B is not sufficient for regulation of tumor necrosis factor-induced interleukin-6 gene expression.

    abstract::After treatment of L929 cells, a murine fibrosarcoma line, with tumor necrosis factor (TNF), a nuclear kappa B-like transcription factor is rapidly induced as identified by gel shift mobility assays using the kappa B-responsive sequence of the immunoglobulin or interleukin-6 (IL-6) genes as a DNA probe. When induction...


    pub_type: 杂志文章


    authors: Patestos NP,Haegeman G,Vandevoorde V,Fiers W

    更新日期:1993-01-01 00:00:00

  • Sensitive to apoptosis gene protein regulates ionizing radiation-induced apoptosis.

    abstract::Organisms exposed to ionizing radiation (IR) undergo increases in the production of reactive oxygen species (ROS), which are determinant components in the induction of apoptosis. Sensitive to apoptosis gene (SAG) encodes a redox-inducible and apoptosis-protective antioxidant protein. This report demonstrates that the ...


    pub_type: 杂志文章


    authors: Kim SY,Yang ES,Lee YS,Lee J,Park JW

    更新日期:2011-02-01 00:00:00

  • [Thermosensitive sporulation and extracellular serylprotease mutant of B. subtilis].

    abstract::Isolation and properties of B. subtilis ts 19 mutant, isolated as thermosensitive for sporulation, are described. At the non permissive temperature (42degreesC), the mutant cells are blocked at stage zero of sporulation and do not excrete extracellular enzymes such as serylprotease and esterase. At the permissive temp...


    pub_type: 杂志文章


    authors: Millet J,Larribe M,Aubert JP

    更新日期:1976-01-01 00:00:00

  • Mitochondria and reactive oxygen species in renal cancer.

    abstract::In most cancer cells, the ATP necessary for survival and proliferation is derived from glycolysis rather than from oxidative phosphorylations (OXPHOS) even when oxygen supply would be adequate to sustain them. This phenomenon, named "aerobic glycolysis" by Warburg many years ago, can now be explained by a mechanism up...


    pub_type: 杂志文章,评审


    authors: Hervouet E,Simonnet H,Godinot C

    更新日期:2007-09-01 00:00:00

  • The formation of an intrachain disulfide bond in the leptin protein is necessary for efficient leptin secretion.

    abstract::Leptin is a cytokine secreted by the adipose tissue that is involved in the control of body weight. We previously showed that a point mutation (R105W) in leptin results in leptin deficiency, marked obesity and hypogonadism in humans adults. Expression in COS1 cells showed impaired secretion and intracellular accumulat...


    pub_type: 杂志文章


    authors: Boute N,Zilberfarb V,Camoin L,Bonnafous S,Le Marchand-Brustel Y,Issad T

    更新日期:2004-06-01 00:00:00

  • Role of metallothioneins in superoxide radical generation during copper redox cycling: defining the fundamental function of metallothioneins.

    abstract::In order to demonstrate the in vivo antioxidant properties of metallothioneins (MTs), the bacteria Escherichia coli was used as a cell reactor in which we compared the metal binding and antioxidative functions of MTs from different species, with different structures and polypeptide lengths. No protective effects of cy...


    pub_type: 杂志文章


    authors: Achard-Joris M,Moreau JL,Lucas M,Baudrimont M,Mesmer-Dudons N,Gonzalez P,Boudou A,Bourdineaud JP

    更新日期:2007-12-01 00:00:00

  • Mitochondrial dysfunction in CD47-mediated caspase-independent cell death: ROS production in the absence of cytochrome c and AIF release.

    abstract::Ligation of CD47 by its natural ligand thrombospondin (TSP), or cross-linking by CD47 antibodies, triggers caspase-independent cell death in normal and leukemic cells. This kind of cell death is characterised by the cytoplasmic events of apoptosis including externalisation of phosphatidylserines and mitochondria swell...


    pub_type: 杂志文章


    authors: Roué G,Bitton N,Yuste VJ,Montange T,Rubio M,Dessauge F,Delettre C,Merle-Béral H,Sarfati M,Susin SA

    更新日期:2003-08-01 00:00:00

  • A stochastic approach to serotonergic fibers in mental disorders.

    abstract::Virtually all brain circuits are physically embedded in a three-dimensional matrix of fibers that release 5-hydroxytryptamine (5-HT, serotonin). The density of this matrix varies across brain regions and cortical laminae, and it is altered in some mental disorders, including Major Depressive Disorder and Autism Spectr...


    pub_type: 杂志文章


    authors: Janušonis S,Detering N

    更新日期:2019-06-01 00:00:00

  • Selection of RNA aptamers against mouse embryonic stem cells.

    abstract::Embryonic stem cells (ESCs) are capable of unlimited self-renewal and differentiation into multiple cell types. Recent large-scale analyses have identified various cell surface molecules on ESCs. Some of them are considered to be beneficial markers for characterization of cellular phenotypes and/or play an essential r...


    pub_type: 杂志文章


    authors: Iwagawa T,Ohuchi SP,Watanabe S,Nakamura Y

    更新日期:2012-01-01 00:00:00

  • The cell cycle arrest and the anti-invasive effects of nitrogen-containing bisphosphonates are not mediated by DBF4 in breast cancer cells.

    abstract::Recent work has shown that a DBF4 analog in yeast may be a target of nitrogen-containing bisphosphonates. DBF4 is an essential protein kinase required for DNA replication from primary eukaryotes to humans and appears to play a critical role in the S-phase checkpoint. It is also required for cell migration and cell sur...


    pub_type: 杂志文章


    authors: Mansouri M,Mirzaei SA,Lage H,Mousavi SS,Elahian F

    更新日期:2014-04-01 00:00:00

  • The nutraceutical potential of omega-3 alpha-linolenic acid in reducing the consequences of stroke.

    abstract::Stroke is a worldwide major cause of mortality and morbidity. Preclinical studies have identified over 1000 molecules with brain-protective properties. More than 200 clinical trials have evaluated neuroprotective candidates for ischemic stroke yet, to date almost all failed, leading to a re-analysis of treatment strat...


    pub_type: 杂志文章,评审


    authors: Blondeau N

    更新日期:2016-01-01 00:00:00

  • Alteration of plasma phospholipid fatty acid profile in patients with septic shock.

    abstract::In septic shock patients, alterations of plasma phospholipid fatty acid profile have never been described. The purpose of this monocentric, non-interventional, observational prospective study was to describe this fatty acid profile in the early phase of septic shock in intensive care unit. Thirty-seven adult patients ...


    pub_type: 杂志文章


    authors: Rival T,Cinq-Frais C,Silva-Sifontes S,Garcia J,Riu B,Salvayre R,Genestal M,Caspar-Bauguil S

    更新日期:2013-11-01 00:00:00

  • Pathophysiological functions of cathepsin D: Targeting its catalytic activity versus its protein binding activity?

    abstract::The lysosomal aspartic protease cathepsin D (cath-D) is overexpressed and hyper-secreted by epithelial breast cancer cells. This protease is an independent marker of poor prognosis in breast cancer as it is correlated with the incidence of clinical metastasis. In normal cells, cath-D is localized in intracellular vesi...


    pub_type: 杂志文章,评审


    authors: Masson O,Bach AS,Derocq D,Prébois C,Laurent-Matha V,Pattingre S,Liaudet-Coopman E

    更新日期:2010-11-01 00:00:00

  • N-acetylamino acid utilization by kidney aminoacylase-1.

    abstract::Mammalian aminoacylase-1 (Acy1) participates in the breakdown of N-acetylated amino acids during intracellular protein catabolism. Acy1 is most abundantly expressed in the kidney tubular epithelium. Lately, Acy1 deficiency was identified in children with increased urinary excretion of several N-acetylamino acids. Here...


    pub_type: 杂志文章


    authors: Lindner HA,Täfler-Naumann M,Röhm KH

    更新日期:2008-05-01 00:00:00

  • [Control of glycogen biosynthesis in fetal rat liver].

    abstract::We have studied through the late foetal period of the rat, the evolution of two enzymes involved in glycogen metabolism of the liver : UDPG glycogen synthetase (a and b forms) and branching enzyme. The activities were measured during the development of normal foetuses and of foetuses experimentally deprived of cortico...


    pub_type: 杂志文章


    authors: Gilbert M,Vaillant R

    更新日期:1975-01-01 00:00:00

  • The structure of the gene for ribosomal protein L5 in the archaebacterium Sulfolobus acidocaldarius.

    abstract::The gene for the ribosomal protein L5 from the archaebacterium Sulfolobus acidocaldarius has been isolated and sequenced. The gene codes for a basic protein of molecular weight 29 165 Da. This protein shows substantial similarity to the equivalent protein from other archaebacteria as well as from yeast, and considerab...


    pub_type: 杂志文章


    authors: Yang D,Günther I,Matheson AT,Auer J,Spicker G,Böck A

    更新日期:1991-06-01 00:00:00

  • Redox regulation of cytochrome P450cam mixed function oxidation by putidaredoxin and camphor ligation.

    abstract::A methylene group of camphor is hydroxylated by a P450 monoxygenase system containing in addition to the cytochrome and flavoprotein dehydrogenase a two-iron, two-sulfur redox protein termed putidaredoxin. The regulatory control of electron transport equilibrium occurs via ligation of substrate and redoxin to the cyto...


    pub_type: 杂志文章


    authors: Gunsalus IC,Sligar SG

    更新日期:1976-01-01 00:00:00

  • Two-dimensional 1H NMR spectroscopy of normal and pathological human plasma: complete water suppression and further assignment of resonances.

    abstract::Two-dimensional J-resolved and correlated 1H NMR spectra with complete water suppression have been obtained to further characterize a metabolic pattern for normal and pathological human plasma samples. 1H COSY spectra have been recorded on plasma from 12 patients with cancer in order to check for the possible presence...


    pub_type: 杂志文章


    authors: Kriat M,Confort-Gouny S,Vion-Dury J,Viout P,Cozzone PJ

    更新日期:1992-09-01 00:00:00

  • The mechanics of DNA loops bridged by proteins unveiled by single-molecule experiments.

    abstract::Protein-induced DNA bridging and looping is a common mechanism for various and essential processes in bacterial chromosomes. This mechanism is preserved despite the very different bacterial conditions and their expected influence on the thermodynamic and kinetic characteristics of the bridge formation and stability. O...


    pub_type: 杂志文章,评审


    authors: Tardin C

    更新日期:2017-11-01 00:00:00

  • Residual structure and dynamics in DMSO-d6 denatured dynein light chain protein.

    abstract::Structural and motional features in the denatured state of a protein dictate the early folding events starting from that state and these features vary depending upon the nature of the denaturant used. Here, we have attempted to decipher the early events in the folding of Dynein Light Chain protein (DLC8), starting fro...


    pub_type: 杂志文章


    authors: Chakraborty S,Mohan PM,Hosur RV

    更新日期:2012-01-01 00:00:00

  • Molecular dissection of the 11S REG (PA28) proteasome activators.

    abstract::The proteasome activators known as 11S REG or PA28 were discovered about 10 years ago. They are homo- or heteroheptameric rings that bind to the ends of 20S proteasomes and activate cleavage of peptides but not folded proteins. In this article, we focus on structural features of three homologous REG subunits (termed a...


    pub_type: 杂志文章,评审


    authors: Li J,Rechsteiner M

    更新日期:2001-03-01 00:00:00

  • Is carotenoid ornamentation linked to the inner mitochondria membrane potential? A hypothesis for the maintenance of signal honesty.

    abstract::Several mechanistic hypotheses have been proposed for how carotenoid pigmentation of integumentary structures can serve as an honest signal of individual quality. These hypotheses are founded on proposed links between carotenoids, immuno responsiveness, and oxidative stress, but an absence of biochemical information o...


    pub_type: 杂志文章


    authors: Johnson JD,Hill GE

    更新日期:2013-02-01 00:00:00

  • [Effect of indomethacin on the lactogenic action of prolactin].

    abstract::The action of indomethacin on the lactogenic activity of prolactin has been evaluated usind the technique of rabbit mammary gland organ culture. Indomethacin is totally unable to inhibit prolactin action as estimated by lactose synthetase activity and casein synthesis. These data suggest, as opposed to previous works...


    pub_type: 杂志文章


    authors: Houdebine LM,Lacroix MC

    更新日期:1980-01-01 00:00:00