A role for calcium in Bcl-2 action?

abstract：:The number of proteins known to contain collagen-like triple helical domains is rapidly increasing. The functions of these domains are to provide molecular rods that separate spatially non-triple helical domains with varied properties and structures and to permit lateral interactions between molecules. Two-thirds of t...

journal_title：Biochimie

authors： van der Rest M,Garrone R

更新日期：1990-06-01 00:00:00

abstract：:In parallel with crystallographic studies of ribosomes from Thermus thermophilus, a long-term program on the crystallization and structural investigations of ribosomal proteins from the same microorganism has been started at the Institute of Protein Research (Pushchino, Russia). At present, more than half of the indiv...

journal_title：Biochimie

authors： Garber MB,Agalarov SC,Eliseikina IA,Fomenkova NP,Nikonov SV,Sedelnikova SE,Shikaeva OS,Vasiliev D,Zhdanov AS,Liljas A

更新日期：1992-04-01 00:00:00

abstract：:Phospholipid metabolism of 3T3 mouse fibroblasts has been studied after serum stimulation of arrested cells. The study of [32Pi] incorporation shows : a) in the case of PE and PC an early peak of incorporation in the G1 phase of the cell cycle, 6 hours after serum addition; b) in the case of PI an intense initial incr...

journal_title：Biochimie

authors： Dubois C,Rampini C

更新日期：1978-01-01 00:00:00

abstract：:The central glycolytic and oxidative pathways and the ATP-producing mechanisms differ in sane and malignant cells by their regulation and dynamics. Fast-growing, poorly-differentiated cancer cells characteristically show high aerobic glycolysis. In the same way, cholesterol biosynthesis, which occurs by normal pathway...

journal_title：Biochimie

authors： Baggetto LG

更新日期：1992-11-01 00:00:00

abstract：:Botulinum neurotoxins (BoNT, serotypes A-G) and tetanus neurotoxin (TeNT) are bacterial proteins that comprise a light chain (M(r) approximately 50) disulfide linked to a heavy chain (M(r) approximately 100). By inhibiting neurotransmitter release at distinct synapses, these toxins cause two severe neuroparalytic dise...

journal_title：Biochimie

authors： Humeau Y,Doussau F,Grant NJ,Poulain B

更新日期：2000-05-01 00:00:00

abstract：:RNA-protein crosslinks were introduced into the 40S ribosomal subunits from Saccharomyces cerevisiae by mild UV treatment. Proteins crosslinked to the 18S rRNA molecule were separated from free proteins by repeated extraction of the treated subunits and centrifugation in glycerol gradients. After digestion with RNase ...

journal_title：Biochimie

authors： Xiang RH,Lee JC

更新日期：1989-11-01 00:00:00

abstract：:Two methods were used to estimate the intracellular phospholipase activity in rat heart: one using exogenous radioactive substrate dispersed as unilamellar vesicles; the other using endogenous membrane hydrolysis and subsequent phospholipid and lysophospholipid separation by high-performance liquid chromatography and ...

journal_title：Biochimie

authors： Termine E,Léonardi J,Lafont H,Nalbone G

更新日期：1987-03-01 00:00:00

abstract：:Natural bovine seminal RNase possesses a potent antitumor action. We have mutagenized monomeric bovine pancreatic RNase A, devoid of any cytotoxic action, to insert residues present at corresponding positions in the subunit of dimeric, antitumor, seminal RNase. Like naturally dimeric seminal RNase, the mutant dimeric ...

journal_title：Biochimie

authors： Cafaro V,Bracale A,Formiggini F,Notomista E,D'Alessio G,Di Donato A

更新日期：1998-11-01 00:00:00

abstract：:Previous work from this laboratory has shown that T7 RNA polymerase outpaces ribosomes in vivo, generating naked mRNA stretches which may be nuclease-sensitive. In particular, lacZ transcripts synthesised this way are highly unstable and yield little beta-galactosidase. We have argued that most of these transcripts ar...

journal_title：Biochimie

authors： Lopez PJ,Dreyfus M

更新日期：1996-01-01 00:00:00

abstract：:In this work, were studied the conditions of erucic acid (cis-docosenoic, n-9) shortening by using Rat liver mitochondrial preparations which were incubated in vitro with [14-14C] erucic acid (22:1), with inhibitors of the respiratory chain (rotenone, cyanide) or not, with activators of either the shortening reaction ...

journal_title：Biochimie

authors： Clouet P,Henninger C,Bezard J

更新日期：1982-10-01 00:00:00

abstract：:Surfactants carrying either a hydrocarbon or a fluorocarbon alkyl chain have been synthesized. The polar head was either tris(hydroxymethyl)acrylamidomethane (THAM), telomerized THAM, or a glycosylated THAM moiety. The aqueous solubility of some of these molecules was increased by oxidizing to a sulfoxide the thioethe...

journal_title：Biochimie

authors： Chabaud E,Barthélémy P,Mora N,Popot JL,Pucci B

更新日期：1998-05-01 00:00:00

abstract：:Although halogenated hydrocarbons are noted for low chemical reactivity, small amounts are toxic to humans. Cytochromes P450 have been implicated in transforming these compounds to more reactive species, under anaerobic conditions, through reduction at the heme. A significant amount of effort has been directed toward ...

journal_title：Biochimie

authors： Manchester JI,Ornstein RL

更新日期：1996-01-01 00:00:00

abstract：:Canine S100A12 (cS100A12) is a calcium-binding protein of the S100 superfamily of EF-hand proteins, and its expression is restricted to neutrophils and monocytes. Interaction of S100A12 with the receptor for advanced glycation end products (RAGE) has been suggested to play a central role in inflammation. Moreover, S10...

journal_title：Biochimie

authors： Heilmann RM,Suchodolski JS,Steiner JM

更新日期：2010-12-01 00:00:00

abstract：:The aim of the present work was to establish the thermodynamic and functional differences in the protein-protein interactions between the components of the P450-dependent mitochondrial (mit) and microsomal (mic) monooxygenase systems using 12 different isoforms of cytochromes P450 and two redox partners, NADPH-depende...

journal_title：Biochimie

authors： Yablokov EO,Sushko TA,Ershov PV,Florinskaya AV,Gnedenko OV,Shkel TV,Grabovec IP,Strushkevich NV,Kaluzhskiy LA,Usanov SA,Gilep AA,Ivanov AS

更新日期：2019-07-01 00:00:00

abstract：:rBmTI-A is a recombinant serine protease inhibitor that belongs to the Kunitz-BPTI family and that was cloned from Rhipicephalus microplus tick. rBmTI-A has inhibitory activities on bovine trypsin, human plasma kallikrein, human neutrophil elastase and plasmin with dissociation constants in nM range. It is characteriz...

journal_title：Biochimie

authors： Bomediano Camillo LM,Ferreira GC,Duran AFA,da Silva FRS,Garcia W,Scott AL,Sasaki SD

更新日期：2020-12-24 00:00:00

abstract：:The biosynthesis of the beta-lactam antibiotic penicillin is an excellent model for the study of secondary metabolites produced by filamentous fungi due to the good background knowledge on the biochemistry and molecular genetics of the beta-lactam producing microorganisms. The three genes (pcbAB, pcbC, penDE) encoding...

journal_title：Biochimie

authors： Kosalková K,García-Estrada C,Ullán RV,Godio RP,Feltrer R,Teijeira F,Mauriz E,Martín JF

更新日期：2009-02-01 00:00:00

abstract：:1. Ghe kinetic and binding parameters of adenine-nucleotide transport have been studied in mitochondria isolated from yeast cells in which the mitochondrial protein-synthetizing system had been inhibited by growth in the presence of erythromycin. These parameters have also been studied in promitochondria isolated from...

journal_title：Biochimie

authors： Lauquin G,Lunardi J,Vignais PV

更新日期：1976-01-01 00:00:00

abstract：:Sm and Sm-like (Lsm) proteins are considered as an evolutionary conserved family involved in RNA metabolism in organisms from bacteria and archaea to human. Currently, the function of Sm-like archaeal proteins (SmAP) is not well understood. Here, we report the crystal structures of SmAP proteins from Sulfolobus acidoc...

journal_title：Biochimie

authors： Lekontseva N,Mikhailina A,Fando M,Kravchenko O,Balobanov V,Tishchenko S,Nikulin A

更新日期：2020-08-01 00:00:00

abstract：:Directed mutagenesis has been used to study the nicotinamide subsite of the glycolytic NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Residue Asn313 is involved together with the carboxyamide moiety of the nicotinamide ring in a complex network of hydrogen bonding interactions which fix the position o...

journal_title：Biochimie

authors： Corbier C,Mougin A,Mely Y,Adolph HW,Zeppezauer M,Gerard D,Wonacott A,Branlant G

更新日期：1990-08-01 00:00:00

abstract：:Over the last decades, mesenchymal stem cells (MSCs) have been extensively studied with regard to their potential applications in regenerative medicine. In rheumatic diseases, MSC-based therapy is the subject of great expectations for patients who are refractory to proposed treatments such as rheumatoid arthritis (RA)...

journal_title：Biochimie

authors： Maumus M,Jorgensen C,Noël D

更新日期：2013-12-01 00:00:00

abstract：:Protein l-isoaspartyl-O-methyltransferase (PIMT) is an ubiquitous enzyme widely distributed in cells and plays a role in the repair of deamidated and isomerized proteins. In this study, we show that this enzyme is present in cytosolic extract of Vibrio cholerae, an enteric pathogenic Gram-negative bacterium and is enz...

journal_title：Biochimie

authors： Chatterjee T,Pal A,Chakravarty D,Dey S,Saha RP,Chakrabarti P

更新日期：2013-04-01 00:00:00

abstract：:The subunit composition of the myosin molecule which is built up from 3 pairs of identical polypeptide chains (2 heavy chains and 2 pairs of light chains), gives it the appearance of having symmetric structure. This homodimeric arrangement in the molecule is in fact asymmetric in its construction as a result of the na...

journal_title：Biochimie

authors： Schaub MC,Watterson JG

更新日期：1981-04-01 00:00:00

abstract：:The phosphotriesterase PTE, identified in the soil bacterium Pseudomonas diminuta, is thought to have evolved in the last several decades to degrade the pesticide paraoxon with proficiency approaching the limit of substrate diffusion (k(cat)/K(M) of 4 x 10(7)M(-1)s(-1)). It belongs to the amidohydrolase superfamily, b...

journal_title：Biochimie

authors： Porzio E,Merone L,Mandrich L,Rossi M,Manco G

更新日期：2007-05-01 00:00:00

abstract：:The structure of the F0 part of ATP synthases from E. coli and Neurospora crassa was analyzed by hydrophobic surface labeling with [125I]TID. In the E. coli F0 all three subunits were freely accessible to the reagent, suggesting that these subunits are independently integrated in the membrane. Labeled amino acid resid...

journal_title：Biochimie

authors： Hoppe J,Sebald W

更新日期：1986-03-01 00:00:00

abstract：:We have previously proposed that the rate of ribosome function during balanced growth in E. coli, expressed as the rate of peptide chain elongation, is adjusted by a feedback mechanism: whenever that rate is submaximal (i.e. below 22 amino acid residues polymerized per active ribosome at 37 degrees C), the feedback si...

journal_title：Biochimie

authors： Bremer H,Dennis P

更新日期：2008-03-01 00:00:00

abstract：:A DNA sequence encoding a protein with predicted EF-hand and dynein light chain binding domains was identified in a Fasciola hepatica EST library. Sequence analysis of the encoded protein revealed that the most similar known protein was the Fasciola gigantica protein FgCaBP3 and so this newly identified protein was na...

journal_title：Biochimie

authors： Banford S,Drysdale O,Hoey EM,Trudgett A,Timson DJ

更新日期：2013-04-01 00:00:00

abstract：:Administration of beta-adrenergic agonists to domestic species can lead to skeletal muscle hypertrophy, probably by reducing the rate of myofibrillar protein breakdown. Myofibrillar breakdown is associated with the calcium-dependent proteinase system (calpains I,II and calpastatin) whose activity also changes during b...

journal_title：Biochimie

authors： Bardsley RG,Allcock SM,Dawson JM,Dumelow NW,Higgins JA,Lasslett YV,Lockley AK,Parr T,Buttery PJ

更新日期：1992-03-01 00:00:00

abstract：:Hemorrhage is the most potent manifestation of envenomation by Vipera ammodytes ammodytes (V. a. ammodytes) venom in man. A detailed description of the venom components contributing to this effect is thus medically very important. We have characterized a novel component, termed here VaH3, as a potently hemorrhagic sna...

journal_title：Biochimie

authors： Sajevic T,Leonardi A,Kovačič L,Lang-Balija M,Kurtović T,Pungerčar J,Halassy B,Trampuš-Bakija A,Križaj I

更新日期：2013-06-01 00:00:00

abstract：:Explants from 7, 8, 9, 11, 13-day chick embryonic skin incorporating (3H) Uridine for different periods 1 hr, 3 or 4 hr and a chase with actinomycin) are studied with respect to free (F) or membrane bound (B) cytoplasmic polysomes and to RNA extracted from them. Polysome specific activity decreases at older stages but...

journal_title：Biochimie

authors： Desveaux-Chabrol J

更新日期：1976-01-01 00:00:00

abstract：:The presence of marker genes encoding antibiotic or herbicide resistances in genetically modified plants poses a number of problems. Various techniques are under development for the removal of unwanted marker genes, while leaving required transgenes in place. The aim of this brief review is to describe the principal m...

journal_title：Biochimie

authors： Scutt CP,Zubko E,Meyer P

更新日期：2002-11-01 00:00:00