Isocitrate dehydrogenase 1 from Acinetobacter baummanii (AbIDH1) enzymatic characterization and its regulation by phosphorylation.

abstract：:Interferons (IFNs) were considered for the treatment of patients with multiple sclerosis (MS) after the demonstration, based on small studies, of the efficacy of type IFN beta in decreasing the frequency of exacerbations in relapsing-remitting multiple sclerosis when administered intrathecally, subcutaneously, or intr...

journal_title：Biochimie

authors： Tourbah A,Lyon-Caen O

更新日期：2007-06-01 00:00:00

abstract：:The mitochondria have arisen as a consequence of endosymbiosis of an ancestral α-proteobacterium with a methane-producing archae. The main function of the canonical aerobic mitochondria include ATP generation via oxidative phosphorylation, heme and phospholipid synthesis, calcium homeostasis, programmed cell death, an...

journal_title：Biochimie

authors： Makiuchi T,Nozaki T

更新日期：2014-05-01 00:00:00

abstract：:Knowing the structure of a protein is essential to characterize its function and mechanism at the molecular level. Despite major advances in solving structures experimentally, most membrane protein native conformations remain unknown. This lack of available structures, along with the physical constraints imposed by th...

journal_title：Biochimie

authors： Postic G,Ghouzam Y,Gelly JC

更新日期：2015-08-01 00:00:00

abstract：:The eukaryotic ribosomal protein S15 is a key component of the decoding site in contrast to its prokaryotic counterpart, S19p, which is located away from the mRNA binding track on the ribosome. Here, we determined the oligopeptide of S15 neighboring the A site mRNA codon on the human 80S ribosome with the use of mRNA ...

journal_title：Biochimie

authors： Khairulina J,Graifer D,Bulygin K,Ven'yaminova A,Frolova L,Karpova G

更新日期：2010-07-01 00:00:00

abstract：:The primary kinetic isotope effect of the reaction catalyzed by NAD+-dependent formate dehydrogenase (EC 1.2.1.2.) from the methylotrophic bacterium Pseudomonas sp. 101 has been studied. Analysis of the ratios HVm/DVm and H(Vm/KM)/D(Vm/KM) in the pH range 6.1-7.9 showed that the transfer of hydride ion in ternary enzy...

journal_title：Biochimie

authors： Tishkov VI,Galkin AG,Egorov AM

更新日期：1989-04-01 00:00:00

abstract：:The phosphotriesterase PTE, identified in the soil bacterium Pseudomonas diminuta, is thought to have evolved in the last several decades to degrade the pesticide paraoxon with proficiency approaching the limit of substrate diffusion (k(cat)/K(M) of 4 x 10(7)M(-1)s(-1)). It belongs to the amidohydrolase superfamily, b...

journal_title：Biochimie

authors： Porzio E,Merone L,Mandrich L,Rossi M,Manco G

更新日期：2007-05-01 00:00:00

abstract：:The class I glutamine (Gln) tRNA synthetase interacts with the anticodon and acceptor stem of glutamine tRNA. RNA hairpin helices were designed to probe acceptor stem and anticodon stem-loop contacts. A seven-base pair RNA microhelix derived from the acceptor stem of tRNA(Gln) was aminoacylated by Gln tRNA synthetase....

journal_title：Biochimie

authors： Wright DJ,Martinis SA,Jahn M,Söll D,Schimmel P

更新日期：1993-01-01 00:00:00

abstract：:The Golgi apparatus serves as the major site of glycosylation reactions. Nucleotide sugars which are substrates of the Golgi localized glycosyltransferases are synthesized in the cytoplasm (cell nucleus in case of CMP-sialic acid) and must be transported into the compartment lumen. This transport function is carried o...

journal_title：Biochimie

authors： Gerardy-Schahn R,Oelmann S,Bakker H

更新日期：2001-08-01 00:00:00

abstract：:Recently we have found that cytokine IL-2 and innate immunity protein Tag7 activate cytotoxic lymphocytes that kill HLA-negative tumor cells, inducing both apoptosis and necroptosis. Here we decrypt the processes, taking part in necroptosis execution after FasL-Fas interaction. Necroptosis begins with RIPK1 activation...

journal_title：Biochimie

authors： Sharapova TN,Romanova EA,Sashchenko LP,Yashin DV

更新日期：2018-09-01 00:00:00

abstract：:A number of specific Fab and Fv fragments and their complexes with antigens (avian lysozymes), haptens, and anti-idiotopic Fabs have been studied by immunochemical and crystallographic techniques. Antigen and antibody interact through closely complementary contacting surfaces, without major conformational changes. An ...

journal_title：Biochimie

authors： Tello D,Spinelli S,Souchon H,Saul FA,Riottot MM,Mariuzza RA,Lascombe MB,Houdusse A,Eiselé JL,Fischmann T

更新日期：1990-08-01 00:00:00

abstract：:Herein, we report the purification and biochemical characterization of a novel bi-functional protein proteinase/amylase inhibitor from the dietary leguminous pulse Phaseolus aureus Roxb. (Vigna radiata L.) by means of acetic acid precipitation, salt fractionation, ion-exchange chromatography (DEAE-cellulose) and affin...

journal_title：Biochimie

authors： Haq SK,Atif SM,Khan RH

更新日期：2005-12-01 00:00:00

abstract：:Botulinum neurotoxins (BoNT, serotypes A-G) and tetanus neurotoxin (TeNT) are bacterial proteins that comprise a light chain (M(r) approximately 50) disulfide linked to a heavy chain (M(r) approximately 100). By inhibiting neurotransmitter release at distinct synapses, these toxins cause two severe neuroparalytic dise...

journal_title：Biochimie

authors： Humeau Y,Doussau F,Grant NJ,Poulain B

更新日期：2000-05-01 00:00:00

abstract：:Flavodoxins are one domain alpha/beta electron transfer proteins that participate in photosynthetic reactions. All flavodoxins carry a molecule of flavin mononucleotide (FMN), non-covalently bound, that confers redox properties to the protein. There are two structurally distinct flavodoxins, short ones and long flavod...

journal_title：Biochimie

authors： Maldonado S,Lostao A,Irún MP,Férnandez-Recio J,Gustavo Genzor C,Begoña González E,Rubio JA,Luquita A,Daoudi F,Sancho J

更新日期：1998-10-01 00:00:00

abstract：:The vasoactive hormone bradykinin, its N-and C-terminal fragments and some structural analogues were studied by Circular Dichroism. Conformational features of the peptide can be detected by comparative analysis of the various CD spectra recorded as a function of aqueous pH, solvent and temperature. It is shown that th...

journal_title：Biochimie

authors： Lintner K,Fermandjian S,Regoli D

更新日期：1979-01-01 00:00:00

abstract：:Zika and Dengue viruses have attracted substantial attention from researchers in light of recent outbreaks of Dengue fever and increases in cases of congenital microcephaly in areas with Zika incidence. This review summarizes the current state of knowledge about Zika and Dengue proteases. These enzymes have several in...

journal_title：Biochimie

authors： Majerová T,Novotný P,Krýsová E,Konvalinka J

更新日期：2019-11-01 00:00:00

abstract：:This review discusses the regulation of a key controlling step in the initiation of protein synthesis, the binding of mRNA to ribosomes. Particular focus is given to the phosphorylation of the cap-binding factor, eIF-4E, and the role of this factor in the regulation of cell growth. ...

journal_title：Biochimie

authors： Sonenberg N

更新日期：1994-01-01 00:00:00

abstract：:Lysosomal cysteine proteinases were fractionated from partially purified rat muscle lysosomes. By gel filtration on Sephadex G75, cathepsin D was separated from two thiol-requiring proteolytic fractions of Mr 25 000 and 55 000, respectively. By chromatofocusing, the first fraction (Mr = 25 000) was resolved into three...

journal_title：Biochimie

authors： Obled A,Ouali A,Valin C

更新日期：1984-09-01 00:00:00

abstract：:The poisonous bite by Hippasa partita, a funnel web spider from the Indian subcontinent has been demonstrated to give rise to severe dermo- and myonecrosis. In this work a hemorrhagic metalloprotease, Partitagin was purified from H. partita venom by successive chromatography on Sephadex G-100, DEAE Sephadex A-50 and B...

journal_title：Biochimie

authors： Nagaraju S,Girish KS,Fox JW,Kemparaju K

更新日期：2007-11-01 00:00:00

abstract：:Pleurocidin (GWGSFFKKAAHVGKHVGKAALTHYL-NH(2)), found in skin mucous secretions of the winter flounder Pleuronectes americanus, is known to possess a high potency and broad-spectrum antimicrobial peptide without cytotoxicity. In this study, to investigate the impact of pleurocidin on apoptotic progress, we observed mor...

journal_title：Biochimie

authors： Cho J,Lee DG

更新日期：2011-10-01 00:00:00

abstract：:Thyroid peroxidase (TPO) is the key enzyme involved in thyroid hormone synthesis. Autoantibodies to TPO (TPOAbs) are a hallmark of autoimmune thyroid disease (AITD). Here, we highlight recent progress over several years in understanding TPO biochemistry and function in various pathologies. TPO undergoes complex post-t...

journal_title：Biochimie

authors： Godlewska M,Banga PJ

更新日期：2019-05-01 00:00:00

abstract：:Glycolipids constitute a class of molecules with various biological activities. Among them, sugar-based bolaamphiphiles characterized by their biocompatibility, biodegradability and lower toxicity, became interesting for the development of efficient and low cost lipid-based drug delivery systems. Their activity seems ...

journal_title：Biochimie

authors： Nasir MN,Crowet JM,Lins L,Obounou Akong F,Haudrechy A,Bouquillon S,Deleu M

更新日期：2016-11-01 00:00:00

abstract：:In recent years an increasing number of inherited decreases in man has been identified in which there is an impairment of one or more peroxisomal functions. Sofar 15 different peroxisomal disorders have been identified which can be subdivided into three distinct groups depending upon whether there is a generalized (gr...

journal_title：Biochimie

authors： Wanders RJ,Schutgens RB,Barth PG,Tager JM,van den Bosch H

更新日期：1993-01-01 00:00:00

abstract：:A methylene group of camphor is hydroxylated by a P450 monoxygenase system containing in addition to the cytochrome and flavoprotein dehydrogenase a two-iron, two-sulfur redox protein termed putidaredoxin. The regulatory control of electron transport equilibrium occurs via ligation of substrate and redoxin to the cyto...

journal_title：Biochimie

authors： Gunsalus IC,Sligar SG

更新日期：1976-01-01 00:00:00

abstract：:Parkinson's disease (PD), a neurodegenerative disorder, is characterized by a loss of dopaminergic neurons in the substantia nigra (SN) of the brain and it is well known that the pathogenesis of PD is related to a number of risk factors including oxidative stress. Antioxidant 1 (ATOX1) protein plays a crucial role in ...

journal_title：Biochimie

authors： Eum WS,Shin MJ,Lee CH,Yeo HJ,Yeo EJ,Choi YJ,Kwon HJ,Kim DS,Kwon OS,Lee KW,Han KH,Park J,Kim DW,Choi SY

更新日期：2019-01-01 00:00:00

abstract：:Several mechanistic hypotheses have been proposed for how carotenoid pigmentation of integumentary structures can serve as an honest signal of individual quality. These hypotheses are founded on proposed links between carotenoids, immuno responsiveness, and oxidative stress, but an absence of biochemical information o...

journal_title：Biochimie

authors： Johnson JD,Hill GE

更新日期：2013-02-01 00:00:00

abstract：:Insulin stimulates protein synthesis in skeletal muscle of young animals and has been reported to exert similar effects on a variety of mammalian cell types in culture. However, with chick embryo fibroblasts we found that the extent of this effect was very sensitive to the culture conditions of the cells prior to the ...

journal_title：Biochimie

authors： Jeffrey IW,Pain VM

更新日期：1993-01-01 00:00:00

abstract：:Recent work has shown that a DBF4 analog in yeast may be a target of nitrogen-containing bisphosphonates. DBF4 is an essential protein kinase required for DNA replication from primary eukaryotes to humans and appears to play a critical role in the S-phase checkpoint. It is also required for cell migration and cell sur...

journal_title：Biochimie

authors： Mansouri M,Mirzaei SA,Lage H,Mousavi SS,Elahian F

更新日期：2014-04-01 00:00:00

abstract：:Coumarins are a group of natural compounds widely distributed in plants. Of late, coumarins and their derivatives have grabbed much attention from the pharmacological and pharmaceutical arena due to their broad range of therapeutical qualities. A coumarin derivative 4-methylesculetin (4-ME) has known to possess effect...

journal_title：Biochimie

authors： Hemshekhar M,Sunitha K,Thushara RM,Sebastin Santhosh M,Shanmuga Sundaram M,Kemparaju K,Girish KS

更新日期：2013-06-01 00:00:00

abstract：:In order to demonstrate the in vivo antioxidant properties of metallothioneins (MTs), the bacteria Escherichia coli was used as a cell reactor in which we compared the metal binding and antioxidative functions of MTs from different species, with different structures and polypeptide lengths. No protective effects of cy...

journal_title：Biochimie

authors： Achard-Joris M,Moreau JL,Lucas M,Baudrimont M,Mesmer-Dudons N,Gonzalez P,Boudou A,Bourdineaud JP

更新日期：2007-12-01 00:00:00

abstract：:Beta-turmerin from turmeric (Curcuma longa) waste grits obtained after extraction of curcumin was purified by successive gel permeation chromatography. Homogeneity of beta-turmerin was confirmed by its movement as single band both in SDS-PAGE and as well as in native (basic) PAGE. The apparent molecular mass is approx...

journal_title：Biochimie

authors： Smitha S,Dhananjaya BL,Dinesha R,Srinivas L

更新日期：2009-09-01 00:00:00