Biochemical characterization, stability studies and N-terminal sequence of a bi-functional inhibitor from Phaseolus aureus Roxb. (Mung bean).

abstract：:Information on the subcellular localization of bacterial proteins is essential for protein function prediction, genome annotation and drug design. Here we proposed a novel approach to predict the subcellular localization of bacterial proteins by fusing features from position-specific score matrix (PSSM), Gene Ontology...

journal_title：Biochimie

authors： Li L,Yu S,Xiao W,Li Y,Li M,Huang L,Zheng X,Zhou S,Yang H

更新日期：2014-09-01 00:00:00

abstract：:The effect of bis-(p-nitrophenyl)phosphate on various glycosyltransferases involved in protein glycosylation (sialyl-, fucosyl-, galactosyl-, mannosyl- and glucosyltransferases) have been studied using crude enzyme preparations solubilized from rat spleen lymphocytes. Bis-(p-nitrophenyl)phosphate appears as a common i...

journal_title：Biochimie

authors： Guette F,Cacan R,Montreuil J,Verbert A

更新日期：1983-10-01 00:00:00

abstract：:The human liver fatty acid binding protein (hFABP1) participates in cellular long-chain fatty acid trafficking and regulation of lipid metabolism and changes in hFABP1 are associated with an increased risk for type 2 diabetes, cardiovascular disease (CVD), and metabolic syndromes. Gene regulation of hFABP1 is not full...

journal_title：Biochimie

authors： Wu YL,Peng XE,Wang D,Chen WN,Lin X

更新日期：2012-02-01 00:00:00

abstract：:In most cases, anti-protein aptamers are selected by systematic evolution of ligands by exponential enrichment (SELEX) using purified recombinant protein targets. Cell surface proteins, however, are not easy targets for SELEX due to the difficulties associated with their purification. Here, we developed a novel SELEX ...

journal_title：Biochimie

authors： Ohuchi SP,Ohtsu T,Nakamura Y

更新日期：2006-07-01 00:00:00

abstract：:Leishmania parasites cause two million new cases of leishmaniasis each year with several hundreds of millions of people at risk. Due to the paucity and shortcomings of available drugs, we have undertaken the crystal structure determination of a key enzyme from Leishmania major in hopes of creating a platform for the r...

journal_title：Biochimie

authors： Larson ET,Kim JE,Zucker FH,Kelley A,Mueller N,Napuli AJ,Verlinde CL,Fan E,Buckner FS,Van Voorhis WC,Merritt EA,Hol WG

更新日期：2011-03-01 00:00:00

abstract：:DNA mismatch repair (MMR) is an important replication error avoidance mechanism that prevents mutation. The association of defective MMR with familial and sporadic gastrointestinal and endometrial cancer has been acknowledged for some years. More recently, it has become apparent that MMR defects are common in acute my...

journal_title：Biochimie

authors： Karran P,Offman J,Bignami M

更新日期：2003-11-01 00:00:00

abstract：:The oxidation of beta-D-glucose with glucose oxidase generally requires oxygen, which, under normal conditions is present at low concentrations in the reaction medium. Experiments show that glucose oxidase is no longer saturated by oxygen at enzyme concentrations greater than 0.4 mg.ml1. This is due to the decrease in...

journal_title：Biochimie

authors： Boudrant J,Cheftel C

更新日期：1975-01-01 00:00:00

abstract：:Botulinum neurotoxins (BoNT, serotypes A-G) and tetanus neurotoxin (TeNT) are bacterial proteins that comprise a light chain (M(r) approximately 50) disulfide linked to a heavy chain (M(r) approximately 100). By inhibiting neurotransmitter release at distinct synapses, these toxins cause two severe neuroparalytic dise...

journal_title：Biochimie

authors： Humeau Y,Doussau F,Grant NJ,Poulain B

更新日期：2000-05-01 00:00:00

abstract：:A major incentive in inhibitor research is that control of limited proteolysis constitutes a valuable pharmacological tool. Protease inhibitors have proved to be successful in influencing pathogenesis in many experimental models but a breakthrough to use in human therapy has mainly been restricted to aprotinin and ang...

journal_title：Biochimie

authors： Scharpe S,De Meester I,Hendriks D,Vanhoof G,van Sande M,Vriend G

更新日期：1991-01-01 00:00:00

abstract：:Phosphatidic acid phosphohydrolase-1 (PAP-1) activity is reversibly inhibited by fatty acids and their acyl-CoA esters and it appears paradoxical that these effectors have been reported to increase the liver's esterification capacity by translocating the rate-limiting enzyme PAP-1 from cytosol to the endoplasmic retic...

journal_title：Biochimie

authors： Elabbadi N,Day CP,Gamouh A,Zyad A,Yeaman SJ

更新日期：2005-05-01 00:00:00

abstract：:SR-PSOX can function as a scavenger receptor, a chemokine and an adhesion molecule, and it could be an interesting player in the formation of atherosclerotic lesions. Our previous studies demonstrated that basic amino acid residues in the chemokine domain of SR-PSOX are critical for its functions. In this study the co...

journal_title：Biochimie

authors： Liu W,Yin L,Dai Y

更新日期：2013-02-01 00:00:00

abstract：:The intervention of pyruvate in glucose metabolism was investigated during hypoxic stress in tumour cell cultures having respiratory capacities under normoxic conditions. Results obtained with nuclear magnetic resonance (NMR) spectroscopy showed that, under normoxic conditions, rat glioma C6 and human hepatoma Hep G2 ...

journal_title：Biochimie

authors： Perrin A,Roudier E,Duborjal H,Bachelet C,Riva-Lavieille C,Leverve X,Massarelli R

更新日期：2002-10-01 00:00:00

abstract：:We are reporting here an analysis of the chymotryptic peptides obtained from the tryptic heme-binding fragment of flavocytochrome b2 (cytochrome b2 core). These results completely establish the sequence of the 96 residue-long fragment, for which preliminary evidence has been published before [24]. We also report full ...

journal_title：Biochimie

authors： Guiard B,Lederer F

更新日期：1976-01-01 00:00:00

abstract：:The cell reproduction cycle of parasitic wall-free bacteria, mycoplasma, is reviewed. DNA replication of Mycoplasma capricolum starts at a fixed site neighboring the dnaA gene and proceeds to both directions after a short arrest in one direction. The initiation frequency fits to the slow speed of replication fork and ...

journal_title：Biochimie

authors： Miyata M,Seto S

更新日期：1999-08-01 00:00:00

abstract：:The 11 kb las locus, present on the 50 kb plasmid pCIM1, specifies the production of the lantibiotic lactocin S in Lactobacillus sakei L45. The gene cluster is organized into two oppositely orientated operons, lasAMNTUVPJW (lasA-W) and lasXY, the former of which contains the biosynthetic, immunity and transport genes....

journal_title：Biochimie

authors： Rawlinson EL,Nes IF,Skaugen M

更新日期：2002-05-01 00:00:00

abstract：:Total poly (A+) RNA has been extracted from rat pheochromocytoma and translated in vitro by means of a reticulocyte lysate system. We show that two antisera, prepared against pig kidney DOPA decarboxylase (DDC) or rat pheochromocytoma DDC, immunoprecipitate an in vitro synthetized 50 kDa polypeptide identified as DDC ...

journal_title：Biochimie

authors： Bruneau G,Krieger-Poullet M,Coge F,Borri-Voltattorni C,Gros F,Thibault J

更新日期：1990-01-01 00:00:00

abstract：:Using natural inducers of beta-glucuronidase, methyl-glucuronide and fructuronate, under gratuitous conditions (without metabolic conversion of these two compounds), we corroborate the fact that both molecules are required simultaneously in order to derepress the enzyme synthesis to a maximum level. Structurally relat...

journal_title：Biochimie

authors： Mandrand-Berthelot MA,Novel G,Novel M

更新日期：1977-01-01 00:00:00

abstract：:Recombinant plasmids containing head-to-tail copies of different coat-protein replacement genomes of wheat dwarf virus (WDV) were used to study the mechanism leading to the release of replicating unit-length molecules in suspension culture cells of Triticum monococcum. For plasmids bearing two complete genomes, the vi...

journal_title：Biochimie

authors： Heyraud F,Matzeit V,Schaefer S,Schell J,Gronenborn B

更新日期：1993-01-01 00:00:00

abstract：:LamB, an integral outer membrane protein of E coli K12, is highly resistant to protease digestion. We had previously genetically inserted a foreign sequence corresponding to an epitope from the poliovirus next to amino acids 146, 153, 189, and 374 of LamB. In 3 cases (sites 146, 153, 374), insertion of the foreign pep...

journal_title：Biochimie

authors： Ronco J,Charbit A,Hofnung M

更新日期：1990-02-01 00:00:00

abstract：:Phospholipases A(2) (PLA(2)s) and its generation of second messengers play an important role in signal transduction, cell proliferation, cell survival and gene expression. At low concentrations mediators of PLA(2) activity have a variety of physiological effects whereas high levels of PLA(2) and its metabolites are ge...

journal_title：Biochimie

authors： Wang J,Kolko M

更新日期：2010-06-01 00:00:00

abstract：:After treatment of L929 cells, a murine fibrosarcoma line, with tumor necrosis factor (TNF), a nuclear kappa B-like transcription factor is rapidly induced as identified by gel shift mobility assays using the kappa B-responsive sequence of the immunoglobulin or interleukin-6 (IL-6) genes as a DNA probe. When induction...

journal_title：Biochimie

authors： Patestos NP,Haegeman G,Vandevoorde V,Fiers W

更新日期：1993-01-01 00:00:00

abstract：:Experimental Lyme arthritis provides a mouse model for exploring the development of pathology following infection of C3H mice with Borrelia burgdorferi. Infected mice develop a reliable inflammatory arthritis of the ankle joint with severity that typically peaks around two to three weeks post-infection and then underg...

journal_title：Biochimie

authors： Brown CR,Dennis EA

更新日期：2017-10-01 00:00:00

abstract：:Pentatricopeptide repeat (PPR) proteins are a large family of modular RNA-binding proteins which mediate several aspects of gene expression primarily in organelles but also in the nucleus. These proteins facilitate processing, splicing, editing, stability and translation of RNAs. While major advances in PPR research h...

journal_title：Biochimie

authors： Manna S

更新日期：2015-06-01 00:00:00

abstract：:The number of proteins known to contain collagen-like triple helical domains is rapidly increasing. The functions of these domains are to provide molecular rods that separate spatially non-triple helical domains with varied properties and structures and to permit lateral interactions between molecules. Two-thirds of t...

journal_title：Biochimie

authors： van der Rest M,Garrone R

更新日期：1990-06-01 00:00:00

abstract：:The ascomycete Cladosporium herbarum is a prominent fungal inducer of Type I allergy. The only major allergen identified so far is Cla h 8, a NADP-dependent mannitol dehydrogenase (MtDH). MtDH, a cytoplasmic protein of 28.5kDa, belongs to the Short chain Dehydrogenases/Reductases (SDR), acting as a NADP-dependent oxid...

journal_title：Biochimie

authors： Nüss D,Goettig P,Magler I,Denk U,Breitenbach M,Schneider PB,Brandstetter H,Simon-Nobbe B

更新日期：2010-08-01 00:00:00

abstract：:Bothropstoxin-I (BthTx-I) is a homodimeric Lys49-PLA2 from the venom of the snake Bothrops jararacussu, which lacks hydrolytic activity against phospholipid substrates, yet permeabilizes membranes by a Ca2+-independent mechanism. The interaction of the BthTx-I with model membranes has been studied by intrinsic tryptop...

journal_title：Biochimie

authors： Ferreira TL,Ruller R,Chioato L,Ward RJ

更新日期：2008-09-01 00:00:00

abstract：:The combination of cDNA and RNA sequencing techniques has enabled determination of the complete sequence of one of the mRNAs coding for the precursor of the small subunit of ribulose bisphosphate carboxylase of Nicotiana sylvestris. In this 898-nucleotide-long mRNA, 540 nucleotides code for the entire 180-amino-acid-l...

journal_title：Biochimie

authors： Pinck M,Guilley E,Durr A,Hoff M,Pinck L,Fleck J

更新日期：1984-07-01 00:00:00

abstract：:An alkaline aminopeptidase was isolated from the culture medium of Penicillium roqueforti. The enzyme was purified by ammonium sulfate precipitation, filtration on Bio-Gel P-100, chromatography on D.E.A.E.-cellulose and hydroxylapatite, filtration on Bio-Gel P-150 and electrofusing. The purified preparation was homoge...

journal_title：Biochimie

authors： Gripon JC

更新日期：1977-01-01 00:00:00

abstract：:The vasoactive hormone bradykinin, its N-and C-terminal fragments and some structural analogues were studied by Circular Dichroism. Conformational features of the peptide can be detected by comparative analysis of the various CD spectra recorded as a function of aqueous pH, solvent and temperature. It is shown that th...

journal_title：Biochimie

authors： Lintner K,Fermandjian S,Regoli D

更新日期：1979-01-01 00:00:00

abstract：:The preadipocyte cell lines 3T3-L1 and 3T3-F442A are widely used to study the cellular mechanisms of preadipocyte differentiation and mature adipocyte functions. However, transfection with naked DNA is inefficient in these cell lines. Adenoviral gene transfer is a powerful technique to induce high levels of transgene ...

journal_title：Biochimie

authors： Béréziat V,Moritz S,Klonjkowski B,Decaudain A,Auclair M,Eloit M,Capeau J,Vigouroux C

更新日期：2005-11-01 00:00:00