Creation of targets for proteolytic cleavage in the LamB protein of E coli K12 by genetic insertion of foreign sequences: implications for topological studies.

abstract：:Cytochromes P450 constitute a superfamily of hemoproteins which have evolved from a common ancestor. Recent advances in molecular biology have offered a powerful approach to the research on the multiplicity of P450. Now, every mammalian P450 species can be characterized by heterologous expression of its cDNA. In addit...

journal_title：Biochimie

authors： Yabusaki Y

更新日期：1995-01-01 00:00:00

abstract：:We have analyzed changes in the supercoiling of nucleoid DNA of murine fibroblasts cultured in the presence of hydralazine. The entire DNA attached to the nuclear matrix was extracted from the cells and sedimented in neutral sucrose density gradients containing ethidium bromide. Nucleoids from cells treated with hydra...

journal_title：Biochimie

authors： Weglarz L,Koceva-Chyła A

更新日期：1998-07-01 00:00:00

abstract：:A major tolerance mechanism that functions to replicate damaged genomic DNA across lesions that have escaped elimination by repair mechanism is translesion DNA synthesis (TLS). DNA polymerase kappa (Pol kappa), a specialised low-fidelity DNA polymerase which is able to perform DNA synthesis across several damaged base...

journal_title：Biochimie

authors： Bavoux C,Hoffmann JS,Cazaux C

更新日期：2005-07-01 00:00:00

abstract：:Direct demonstration of the reversible dissociation of native dimeric methionyl-tRNA synthetase from E. coli has been obtained using small angle neutron scattering and deuterated enzyme. Structural parameters of the fully deuterated dimer are very similar to the hydrogenated one. Analysis of the variations of the inte...

journal_title：Biochimie

authors： Dessen P,Zaccai G,Blanquet S

更新日期：1985-06-01 00:00:00

abstract：:Botulinum neurotoxins (BoNT, serotypes A-G) and tetanus neurotoxin (TeNT) are bacterial proteins that comprise a light chain (M(r) approximately 50) disulfide linked to a heavy chain (M(r) approximately 100). By inhibiting neurotransmitter release at distinct synapses, these toxins cause two severe neuroparalytic dise...

journal_title：Biochimie

authors： Humeau Y,Doussau F,Grant NJ,Poulain B

更新日期：2000-05-01 00:00:00

abstract：:Nicotinate phosphoribosyltransferase (NaPRT, EC 2.4.2.11) catalyzes the conversion of nicotinate (Na) to nicotinate mononucleotide, the first reaction of the Preiss-Handler pathway for the biosynthesis of NAD(+). Even though NaPRT activity has been described to be responsible for the ability of Na to increase NAD(+) l...

journal_title：Biochimie

authors： Galassi L,Di Stefano M,Brunetti L,Orsomando G,Amici A,Ruggieri S,Magni G

更新日期：2012-02-01 00:00:00

abstract：:Using natural inducers of beta-glucuronidase, methyl-glucuronide and fructuronate, under gratuitous conditions (without metabolic conversion of these two compounds), we corroborate the fact that both molecules are required simultaneously in order to derepress the enzyme synthesis to a maximum level. Structurally relat...

journal_title：Biochimie

authors： Mandrand-Berthelot MA,Novel G,Novel M

更新日期：1977-01-01 00:00:00

abstract：:The chemical carcinogen N-acetoxy-N-2-acetylaminofluorene (N-AcO-AAF) induces frameshift mutations located within two types of specific sequences (mutation hot spots): i) contiguous guanine sequences and ii) alternating GC sequences. The genetic requirements of these frameshift events were investigated using specific ...

journal_title：Biochimie

authors： Janel-Bintz R,Maenhaut-Michel G,Takahashi M,Fuchs RP

更新日期：1991-04-01 00:00:00

abstract：:The present study was conducted on CD4(+) T cells, isolated from wild type (WT) and PPARalpha(null) mice, in order to assess the mechanism of action of docosahexaenoic acid (DHA), an n-3 fatty acid, in the modulation of two transcription factors, i.e., T-bet and GATA-3, implicated in T-cell differentiation towards, re...

journal_title：Biochimie

authors： Attakpa E,Hichami A,Simonin AM,Sansón EG,Dramane KL,Khan NA

更新日期：2009-11-01 00:00:00

abstract：:The function of glycerophosphate and malate-aspartate shuttles during glucose metabolism in two strains of Ehrlich ascites tumor cells was evaluated by several experimental approaches. The activities of the enzymes involved in these shuttle systems were assayed in the cytosolic and mitochondrial compartments after cel...

journal_title：Biochimie

authors： Sánchez-Jiménez F,Martínez P,Núñez de Castro I,Olavarría JS

更新日期：1985-02-01 00:00:00

abstract：:The binding properties of Mg2+, Mn2+ and Co2+ to yeast mitochondrial oligomycin sensitive ATPase complex are studied, as reflected by their catalytic effect (hydrolysis of ATP or pNPP, a pseudo substrate) or by a physical parameter (atomic absorption, electron paramagnetic reasonance of Mn2+, enhanced fluorescence of ...

journal_title：Biochimie

authors： Jallon JM,Spyridakis A,Cosson J

更新日期：1977-01-01 00:00:00

abstract：:Epigenetic alterations are involved in every step of carcinogenesis. The development of chromatin-modifying agents (CMAs) has provided the ability to fight cancer by reversing these alterations. Currently, four CMAs have been approved for cancer treatment; two DNA demethylating agents and two deacetylase inhibitors. A...

journal_title：Biochimie

authors： Seidel C,Florean C,Schnekenburger M,Dicato M,Diederich M

更新日期：2012-11-01 00:00:00

abstract：:The cellular concentration of cyclic nucleotides is largely dependent upon the activity of the enzymatic system responsible for their degradation: cyclic nucleotide phosphodiesterase. This enzymatic system thus plays a crucial role in the regulation of the multiple functions which are modulated by cyclic nucleotides i...

journal_title：Biochimie

authors： Nemoz G,Prigent AF

更新日期：1984-02-01 00:00:00

abstract：:Bacterial type II fatty acid synthesis (FASII) is a target for novel antibiotic development. All bacteria encode for mechanisms to incorporate exogenous fatty acids, and some bacteria can use exogenous fatty acids to bypass FASII inhibition. Bacteria encode three different mechanisms for activating exogenous fatty aci...

journal_title：Biochimie

authors： Yao J,Rock CO

更新日期：2017-10-01 00:00:00

abstract：:Nanoluciferase (NanoLuc) is a newly developed small luciferase reporter with the brightest bioluminescence reported to date. In the present work, we developed NanoLuc as a novel quantitative protein fusion tag for efficient overexpression in Escherichia coli and ultrasensitive bioluminescent assays using human leukemi...

journal_title：Biochimie

authors： He SX,Song G,Shi JP,Guo YQ,Guo ZY

更新日期：2014-11-01 00:00:00

abstract：:Interferon-gamma is a key cytokine in tumor immunosurveillance. The recently described interferon-producing killer dendritic cell (IKDC), can be distinguished from other innate effectors by its ability to kill a large variety of tumor cells and to produce high amounts of interferon-gamma after encountering tumors in t...

journal_title：Biochimie

authors： Bonmort M,Ullrich E,Mignot G,Jacobs B,Chaput N,Zitvogel L

更新日期：2007-06-01 00:00:00

abstract：:Kissing loop interactions (KLIs) are a common motif that is critical in retroviral dimerization, viroid replication, mRNA, and riboswitches. In addition, KLIs are currently used in a variety of biotechnology applications, such as in aptamer sensors, RNA scaffolds and to stabilize vaccines for therapeutics. Here we des...

journal_title：Biochimie

authors： Carr CE,Marky LA

更新日期：2019-02-01 00:00:00

abstract：:Glycogen synthase I was purified from bovine polymorphonuclear leucocytes (PMNs) by a procedure involving concanavalin A-Sepharose affinity chromatography. The purified glycogen-bound glycogen synthase I had a specific activity of 9.83 U/mg protein and the glycogen free enzyme 21 U/mg protein. Molecular ratio of the n...

journal_title：Biochimie

authors： Rasmussen LH,Pedersen KM,Juhl H

更新日期：1985-06-01 00:00:00

abstract：:The mechanism of action on rabbit red cells of Treponema hyodysenteriae hemolysin was studied using volume analysis and release of hemoglobin. While fixation of the hemolysin on the erythrocytes is temperature independent, it appears that hemolysis is temperature dependent. The kinetics of hemolysis proceed according ...

journal_title：Biochimie

authors： Saheb SA,Lafleur L

更新日期：1980-01-01 00:00:00

abstract：:Murine L5178Y cell ribosomes were dissociated into subunits either with potassium chloride in the presence of puromycin or with the chelating agent EDTA. The proteins of ribosomal subunits obtained by these different methods were compared by means of bidimensional polyacrylamide gel electrophoresis. KCl-derived 60S an...

journal_title：Biochimie

authors： Longuet M,Auger-Buendia MA,Tavitian A

更新日期：1979-01-01 00:00:00

abstract：:Alteration of the epigenome is associated with a wide range of human diseases. Therefore, deciphering the pathways that regulate the epigenetic modulation of gene expression is a major milestone for the understanding of diverse biological mechanisms and subsequently human pathologies. Although often evoked, little is ...

journal_title：Biochimie

authors： Ottaviani A,Gilson E,Magdinier F

更新日期：2008-01-01 00:00:00

abstract：:In parallel with crystallographic studies of ribosomes from Thermus thermophilus, a long-term program on the crystallization and structural investigations of ribosomal proteins from the same microorganism has been started at the Institute of Protein Research (Pushchino, Russia). At present, more than half of the indiv...

journal_title：Biochimie

authors： Garber MB,Agalarov SC,Eliseikina IA,Fomenkova NP,Nikonov SV,Sedelnikova SE,Shikaeva OS,Vasiliev D,Zhdanov AS,Liljas A

更新日期：1992-04-01 00:00:00

abstract：:Estrogen plays an essential role in bone formation, and estrogen modulation dysfunction is tightly associated with postmenopausal osteoporosis (PMOP). The underlying mechanisms of estrogen-mediated osteogenic differentiation have not been well defined. In this study, murine bone marrow mesenchymal stem cells were indu...

journal_title：Biochimie

authors： Gao B,Huang Q,Jie Q,Wang L,Zhang HY,Liu J,Yang L,Luo ZJ

更新日期：2015-03-01 00:00:00

abstract：:Since discovery of high-temperature superconductive (HTS) ceramics by Bednorz and Muller in 1986, there has been an accelerated development of cold technologies in industry, including the domain of NMR detection. The purpose of this paper is to fix ideas about the stage that cryogenic radio frequency (RF) probe techni...

journal_title：Biochimie

authors： Darrasse L,Ginefri JC

更新日期：2003-09-01 00:00:00

abstract：:In around 10% of SARS-CoV-2 infected patients, coronavirus disease-2019 (Covid-19) symptoms are complicated with a severe lung damage called Acute Respiratory Distress Syndrome (ARDS), which is often lethal. ARDS is mainly associated with an uncontrolled overproduction of immune cells and cytokines, called "cytokine s...

journal_title：Biochimie

authors： Weill P,Plissonneau C,Legrand P,Rioux V,Thibault R

更新日期：2020-12-01 00:00:00

abstract：:We previously reported that the rRNA methyltransferases RsmI and RsmH, which are responsible for cytidine dimethylation at position 1402 of 16S rRNA in the decoding center of the ribosome, contribute to Staphylococcus aureus virulence. Here we evaluated other 16S rRNA methyltransferases, including KsgA (RsmA), RsmB/F,...

journal_title：Biochimie

authors： Kyuma T,Kizaki H,Ryuno H,Sekimizu K,Kaito C

更新日期：2015-12-01 00:00:00

abstract：:Insulin stimulates protein synthesis in skeletal muscle of young animals and has been reported to exert similar effects on a variety of mammalian cell types in culture. However, with chick embryo fibroblasts we found that the extent of this effect was very sensitive to the culture conditions of the cells prior to the ...

journal_title：Biochimie

authors： Jeffrey IW,Pain VM

更新日期：1993-01-01 00:00:00

abstract：:The emergence of drug-resistant Mycobacterium tuberculosis (Mtb) stains has escalated the need for developing more efficient drugs and therapeutic strategies against tuberculosis. Here we functionally annotate a secretory mycobacterial asparaginase Rv1538c (MtA) and describe its biochemical properties. MtA primarily e...

journal_title：Biochimie

authors： Kataria A,Patel AK,Kundu B

更新日期：2021-01-05 00:00:00

abstract：:Immobilization of enzymes by crystallization and subsequent cross-linking provide structures characterized by a regular three-dimensional molecular arrangement and a high packing density. Compared to randomly immobilized enzymes, such structures permit more detailed analysis of the variations in kinetic properties ari...

journal_title：Biochimie

authors： Tüchsen E,Hvidt A,Ottesen M

更新日期：1980-01-01 00:00:00

abstract：:Concern about the malicious applications of botulinum neurotoxin has highlighted the need for a new generation of safe and highly potent antitoxins. In this study, we developed and evaluated the preclinical pharmacology and safety of a new F(ab')₂ antitoxin against botulinum neurotoxin serotype A (BoNT/A). As an alter...

journal_title：Biochimie

authors： Yu YZ,Zhang SM,Wang WB,Du Y,Zhu HQ,Wang RL,Zhou XW,Lin JB,Wang S,Yu WY,Huang PT,Sun ZW

更新日期：2010-10-01 00:00:00