Methionyl-tRNA synthetase from E. coli: direct evidence for exchange of protomers in the dimeric enzyme by using deuteration and small-angle neutron scattering.

abstract：:So far, no efficient affinity chromatography for CCK receptor purification has been reported that prevented obtention of sequenceable amounts of purified receptor. In this work, 10% of plasma membrane receptor sites were specifically cross-linked with the photoreactive cleavable agonist 125I-ASD-[Thr28, Ahx31]-CCK-25-...

journal_title：Biochimie

authors： Dufresne M,Poirot S,Jimenez J,Vaysse N,Fourmy D

更新日期：1992-02-01 00:00:00

abstract：:Epigenetic alterations are involved in every step of carcinogenesis. The development of chromatin-modifying agents (CMAs) has provided the ability to fight cancer by reversing these alterations. Currently, four CMAs have been approved for cancer treatment; two DNA demethylating agents and two deacetylase inhibitors. A...

journal_title：Biochimie

authors： Seidel C,Florean C,Schnekenburger M,Dicato M,Diederich M

更新日期：2012-11-01 00:00:00

abstract：:Seven virulent bacteriophages of Streptococcus thermophilus were characterized at the molecular level and classified into 2 subgroups (A and B) by DNA/DNA hybridization experiments and analysis of their structural proteins. Two representatives of subgroups A and B were compared to 3 representatives of Neve's subgroups...

journal_title：Biochimie

authors： Benbadis L,Faelen M,Slos P,Fazel A,Mercenier A

更新日期：1990-12-01 00:00:00

abstract：:Hypertension is the leading cause of preventable mortality worldwide, contributing to over 9 million deaths per annum, predominantly owing to cardiovascular disease. The association of obesity, physical inactivity and alcohol with elevated blood pressure (BP) is firmly established. Weight loss or other dietary strateg...

journal_title：Biochimie

authors： Psara E,Pentieva K,Ward M,McNulty H

更新日期：2020-06-01 00:00:00

abstract：:The family of interferon regulatory transcription factors (IRF) participates in the virus-induced and dsRNA-stimulated transcriptional regulation of either type I IFN genes or a definite set of genes which can also be activated by IFN. In this review, we place emphasis on the role of IRF-3 that associates with the coa...

journal_title：Biochimie

authors： Bragança J,Civas A

更新日期：1998-08-01 00:00:00

abstract：:gamma-Aminobutyraldehyde dehydrogenase from Escherichia coli K-12 has been purified and characterized from cell mutants able to grow in putrescine as the sole carbon and nitrogen source. The enzyme has an Mr of 195,000 +/- 10,000 in its dimeric form with an Mr of 95,000 +/- 1,000 for each subunit, a pH optimum at 5.4 ...

journal_title：Biochimie

authors： Prieto MI,Martin J,Balaña-Fouce R,Garrido-Pertierra A

更新日期：1987-11-01 00:00:00

abstract：:Natural bovine seminal RNase possesses a potent antitumor action. We have mutagenized monomeric bovine pancreatic RNase A, devoid of any cytotoxic action, to insert residues present at corresponding positions in the subunit of dimeric, antitumor, seminal RNase. Like naturally dimeric seminal RNase, the mutant dimeric ...

journal_title：Biochimie

authors： Cafaro V,Bracale A,Formiggini F,Notomista E,D'Alessio G,Di Donato A

更新日期：1998-11-01 00:00:00

abstract：:The major pterin in Tetrahymena pyriformis, strain W, earlier suggested to be L-threo-biopterin and named ciliapterin [1] is now identified as D-threo-neopterin (D-monapterin). This is the first example of a natural D-monapterin. This compound was characterized by its chromatographic behavior, its fluorescence propert...

journal_title：Biochimie

authors： Klein R,Tatischeff I,Tham G,Grolière CA

更新日期：1991-10-01 00:00:00

abstract：:The presence of a fragment of polyA resistant to both T1 and p ribonucleases in mRNAs extracted from wing imaginal disks of an insect, Pieris brassicae, is reported. Its length was approximatively 150 nucleotides. PolyU sepharose affinity chromatography was subsequently used for purification of these polyA(+)mRNA mole...

journal_title：Biochimie

authors： Tarroux P

更新日期：1975-01-01 00:00:00

abstract：:After exhaustive pronase digestion, purification by gel filtration and affinity chromatography on concanavalin A, three glycopeptide fractions were obtained from rat hemopexin. Two fractions (I and II) were concanavalin A non-reactive and one (III) was concanavalin A reactive. On the basis of carbohydrate composition,...

journal_title：Biochimie

authors： Bernard N,Lombart C,Strecker G,Montreuil J,Van Halbeek H,Vliegenthart JF

更新日期：1983-03-01 00:00:00

abstract：:HIV-1 reverse transcriptase uses the host tRNA3(Lys) as a primer for the synthesis of the minus DNA strand. The first event in viral replication is thus the annealing of tRNA to the primer binding site (PBS) in the 5' UTR of the viral RNA. This event requires a major RNA rearrangement which is chaperoned by the viral ...

journal_title：Biochimie

authors： Barraud P,Gaudin C,Dardel F,Tisné C

更新日期：2007-10-01 00:00:00

abstract：:An apparatus for chemi- and bioluminescence measurements is described. The main features are in high sensitivity: for example, 5 10(-12) M adenosine triphosphate, 5 10(-7) M glucose, 2 10(-9) M perhydrol and 4 10(-13) M peroxidase are easily detected; its fast mixing time and the possibility of injecting a reactant, a...

journal_title：Biochimie

authors： Arrio B,Lecuyer B,Dupaix A,Volfin P,Jousset M,Carrette A

更新日期：1980-01-01 00:00:00

abstract：:Human telomeres are intrinsically dynamic structures, with multiple biological processes operating to generate substantial length heterogeneity. Processes that operate specifically at the terminus, that include the end-replication problem coupled with C-strand resection, result in gradual telomere erosion with ongoing...

journal_title：Biochimie

authors： Baird DM

更新日期：2008-01-01 00:00:00

abstract：:Versican and decorin, two proteoglycans (PGs) with contradictory roles in the pathophysiology of cancer, comprise important stromal components in many tumor types and play a crucial role in the progression of cancer. In this study, we provide direct evidence for a significant and stage-related accumulation of versican...

journal_title：Biochimie

authors： Skandalis SS,Theocharis AD,Papageorgakopoulou N,Vynios DH,Theocharis DA

更新日期：2006-09-01 00:00:00

abstract：:The two subunits of the nickel-iron hydrogenase from Desulfovibrio gigas have been purified by preparative sodium dodecyl sulfate polyacrylamide gel electrophoresis and their amino acid compositions have been determined. The N-terminal sequences for 15 residues of the large subunit (Mr 62,000) and 25 residues of the s...

journal_title：Biochimie

authors： Nivière V,Forget N,Bovier-Lapierre G,Bonicel J,Hatchikian C

更新日期：1988-02-01 00:00:00

abstract：:Over the years, imino proton exchange measurements have provided a description of base-pair opening and of properties of internal motions of nucleic acids such as the individual opening of base pairs and the insensitivity of base-pair lifetimes to the nature and stability of neighboring pairs. A recent determination o...

journal_title：Biochimie

authors： Leijon M,Leroy JL

更新日期：1997-12-01 00:00:00

abstract：:Glycogen synthase I was purified from bovine polymorphonuclear leucocytes (PMNs) by a procedure involving concanavalin A-Sepharose affinity chromatography. The purified glycogen-bound glycogen synthase I had a specific activity of 9.83 U/mg protein and the glycogen free enzyme 21 U/mg protein. Molecular ratio of the n...

journal_title：Biochimie

authors： Rasmussen LH,Pedersen KM,Juhl H

更新日期：1985-06-01 00:00:00

abstract：:Nitrogen fixation in A. vinelandii and R. leguminosarum bacteroides shows identical characteristics with respect to the dependence on membrane energization, the sensitivity to uncouplers, the ATP/ADP-ratio, and the dependences on flavodoxinhydroquinone as electrondonor. Although we have been successful in preparing in...

journal_title：Biochimie

authors： Veeger C,Laane C,Scherings G,van Zeeland Wolbers L

更新日期：1978-01-01 00:00:00

abstract：UNLABELLED:Pancreatic cancer (PC) is one of the most lethal human malignancies and a major health problem. Patients diagnosed with PC and treated with conventional approaches have an overall 5-year survival rate of less than 5%. Novel strategies are needed to treat this disease. Herein, we propose a combinatorial strat...

journal_title：Biochimie

authors： Breton CS,Aubry D,Ginet V,Puyal J,Heulot M,Widmann C,Duchosal MA,Nahimana A

更新日期：2015-09-01 00:00:00

abstract：:Cytokinin-requiring tobacco cells were incubated for 10 h in the presence of a labeled cytokinin. N6-benzyl-[2-3H]Ade, and of [8-14C]Ado. After alkaline hydrolysis of total RNA and fractionation of the resulting nucleotides, 80 per cent of the 3H radioactivity of RNA were recovered as the N6-benzyl-Ado nucleotide, cov...

journal_title：Biochimie

authors： Teyssendier de la Serve B,Jouanneau JP

更新日期：1979-01-01 00:00:00

abstract：:rBmTI-A is a recombinant serine protease inhibitor that belongs to the Kunitz-BPTI family and that was cloned from Rhipicephalus microplus tick. rBmTI-A has inhibitory activities on bovine trypsin, human plasma kallikrein, human neutrophil elastase and plasmin with dissociation constants in nM range. It is characteriz...

journal_title：Biochimie

authors： Bomediano Camillo LM,Ferreira GC,Duran AFA,da Silva FRS,Garcia W,Scott AL,Sasaki SD

更新日期：2020-12-24 00:00:00

abstract：:Ceramide synthases (CerSs) are key enzymes in the biosynthesis of ceramides and display a group of at least six different isoenzymes (CerS1-6). Ceramides itself are bioactive molecules. Ceramides with different N-acyl side chains (C(14:0)-Cer - C(26:0)-Cer) possess distinct roles in cell signaling. Therefore, the sele...

journal_title：Biochimie

authors： Schiffmann S,Hartmann D,Fuchs S,Birod K,Ferreiròs N,Schreiber Y,Zivkovic A,Geisslinger G,Grösch S,Stark H

更新日期：2012-02-01 00:00:00

abstract：:Oxazole-containing macrocycles, which include the natural product telomestatin, represent a promising class of anticancer agents that target G-quadruplex DNA. Two synthetic hexaoxazole-containing macrocyclic compounds (HXDV and HXLV-AC) have been characterized with regard to their cytotoxic activities versus human can...

journal_title：Biochimie

authors： Pilch DS,Barbieri CM,Rzuczek SG,Lavoie EJ,Rice JE

更新日期：2008-08-01 00:00:00

abstract：:Glycosphingolipids and sphingolipids of cellular plasma membranes (PMs) reach luminal intra-lysosomal vesicles (LVs) for degradation mainly by pathways of endocytosis. After a sorting and maturation process (e.g. degradation of sphingomyelin (SM) and secretion of cholesterol), sphingolipids of the LVs are digested by ...

journal_title：Biochimie

authors： Sandhoff K

更新日期：2016-11-01 00:00:00

abstract：:In most cancer cells, the ATP necessary for survival and proliferation is derived from glycolysis rather than from oxidative phosphorylations (OXPHOS) even when oxygen supply would be adequate to sustain them. This phenomenon, named "aerobic glycolysis" by Warburg many years ago, can now be explained by a mechanism up...

journal_title：Biochimie

authors： Hervouet E,Simonnet H,Godinot C

更新日期：2007-09-01 00:00:00

abstract：:We have purified, by a three step procedure, a yeast C-nitrosoreductase and studied its action on p-nitroso-N,N-dimethylaniline. Microcalorimetric measurements performed on the microcalorimeter LKB "batch", have been given the number of protons exchanged during the reaction : four protons are involved. Two are given b...

journal_title：Biochimie

authors： Huynh CH

更新日期：1975-01-01 00:00:00

abstract：:The product of the spoIIM gene of Bacillus subtilis is required for complete septum migration and forespore engulfment during sporulation. To investigate whether expression of spoIIM is required in the forespore compartment of the sporangium, we have constructed a new integrational vector, pKSV7, which contains temper...

journal_title：Biochimie

authors： Smith K,Youngman P

更新日期：1992-07-01 00:00:00

abstract：:LipA from Pseudomonas protegens Pf-5 has been proven not to be secreted into the extracytoplasmic space, proposing that it is a membrane protein in virtue of its N-terminal transmembrane domain predicted by the TMHMM 2.0. However, LipA was confirmed to be an intracellular protein through determining the effects of lip...

journal_title：Biochimie

authors： Zha D,Zhang H,Zhang H,Xu L,Yan Y

更新日期：2014-10-01 00:00:00

abstract：:Streptomyces sp. 139 produces a novel exopolysaccharide (EPS) designated Ebosin which can bind IL-1R specifically and exhibits anti-rheumatic arthritis activity in vivo. With the Ebosin biosynthesis gene cluster (ste) consisting of 27 ORFs identified previously the focus of this study was to characterize the protein e...

journal_title：Biochimie

authors： Bai L,Chang M,Shan J,Jiang R,Zhang Y,Zhang R,Li Y

更新日期：2011-09-01 00:00:00

abstract：:Microsomal membranes from potato tubers were extracted by acetone solutions of increasing concentrations (5, 10, 15, 20, 30, 40, 50, 70 and 90 p. cent). Microsomal lipids were progressively extracted: acetone concentrations exceeding 30 p. cent extracted large amounts of membraneous phospholipids (figure). Lipid extra...

journal_title：Biochimie

authors： Jolliot A,Demandre C,Mazliak P

更新日期：1978-01-01 00:00:00