Abstract:
:The oxidation of beta-D-glucose with glucose oxidase generally requires oxygen, which, under normal conditions is present at low concentrations in the reaction medium. Experiments show that glucose oxidase is no longer saturated by oxygen at enzyme concentrations greater than 0.4 mg.ml1. This is due to the decrease in the oxygen concentration of the solution. The value of the oxygen mass transfer coefficients and dissolved oxygen concentrations are determined. These dissolved oxygen concentrations are found to correlate with direct measurements with an oxygen electrode. From this, the Michaelis constant of glucose oxidase for oxygen is calculated. These experiments also show that oxygen is a limiting factor for this reaction.
journal_name
Biochimiejournal_title
Biochimieauthors
Boudrant J,Cheftel Cdoi
10.1016/s0300-9084(75)80160-xkeywords:
subject
Has Abstractpub_date
1975-01-01 00:00:00pages
117-22issue
2eissn
0300-9084issn
1638-6183journal_volume
57pub_type
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