Abstract:
:After treatment of L929 cells, a murine fibrosarcoma line, with tumor necrosis factor (TNF), a nuclear kappa B-like transcription factor is rapidly induced as identified by gel shift mobility assays using the kappa B-responsive sequence of the immunoglobulin or interleukin-6 (IL-6) genes as a DNA probe. When induction was carried out under conditions of increased or decreased cytotoxicity, which correlates with altered IL-6 gene expression, nuclear factor kappa B (NF-kappa B) activation was also demonstrated, but the abundance of the protein/DNA complex observed remained unchanged. Also activation of NF-kappa B as a function of time following TNF treatment did not reveal a correlation between the abundance of the protein/DNA complex and the TNF-induced IL-6 mRNA levels. Moreover, in L929 cells resistant to TNF cytotoxicity, the kappa B-like factor still became fully activated by TNF, although the IL-6 gene was only marginally expressed. In conclusion, discrepancies between the abundance of the activated NF-kappa B-like factor and the IL-6 mRNA production upon treatment with TNF indicate that (an) additional transcription factor(s) and/or (a) regulating mechanism(s) is (are) necessary for fine regulation of the level of IL-6 gene expression in response to cytokine stimulation.
journal_name
Biochimiejournal_title
Biochimieauthors
Patestos NP,Haegeman G,Vandevoorde V,Fiers Wdoi
10.1016/0300-9084(93)90153-jsubject
Has Abstractpub_date
1993-01-01 00:00:00pages
1007-18issue
11eissn
0300-9084issn
1638-6183pii
0300-9084(93)90153-Jjournal_volume
75pub_type
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