Effect of the thermal variation on the molecular weight estimation of the acid phosphatase from Physarum polycephalum.

abstract：:Rat kidney acylase I was characterised by performing site-directed mutagenesis and enzymatic analysis in the presence of various chemical inhibitors. Site-directed mutagenesis on E147 and overexpression of the protein in a bacterial system, revealed the importance of this residue in enzymatic activity, it corresponds ...

journal_title：Biochimie

authors： Durand A,Giardina T,Villard C,Roussel A,Puigserver A,Perrier J

更新日期：2003-10-01 00:00:00

abstract：:The wheat germ translation initiation factor 2 (WGeIF-2) was isolated in a homogeneous state by an efficient procedure and characterized. Its molecular mass, as determined by a gel-filtration method is approximately 150,000 Da. According to SDS-PAGE WGeIF-2 consists of four subunits with M(r) 37,000 (alpha), 40,000 (b...

journal_title：Biochimie

authors： Shaikhin SM,Smailov SK,Lee AV,Kozhanov EV,Iskakov BK

更新日期：1992-05-01 00:00:00

abstract：:In this work, were studied the conditions of erucic acid (cis-docosenoic, n-9) shortening by using Rat liver mitochondrial preparations which were incubated in vitro with [14-14C] erucic acid (22:1), with inhibitors of the respiratory chain (rotenone, cyanide) or not, with activators of either the shortening reaction ...

journal_title：Biochimie

authors： Clouet P,Henninger C,Bezard J

更新日期：1982-10-01 00:00:00

abstract：:Innate immune system is the first line of host defense against invading microorganisms. It relies on a limited number of germline-encoded pattern recognition receptors that recognize conserved molecular structures of microbes, referred to as pathogen-/microbe-associated molecular patterns (PAMPs/MAMPs). Bacterial cell...

journal_title：Biochimie

authors： Ray A,Cot M,Puzo G,Gilleron M,Nigou J

更新日期：2013-01-01 00:00:00

abstract：:Since the initial identification of leptin as the product of the ob gene in 1994, the signaling pathways by which this hormone alters cell physiology have been the subject of extensive investigations. The fact that leptin can induce nitric oxide (NO) production was first demonstrated in studies of the pituitary gland ...

journal_title：Biochimie

authors： Joffin N,Niang F,Forest C,Jaubert AM

更新日期：2012-10-01 00:00:00

abstract：:Insects have developed an efficient host defense against microorganisms, which involves humoral and cellular mechanisms. Numerous data highlight similarities between defense responses of insects and innate immunity of mammals. The fruit fly, Drosophila melanogaster, is a favorable model system for the analysis of the ...

journal_title：Biochimie

authors： Levy F,Rabel D,Charlet M,Bulet P,Hoffmann JA,Ehret-Sabatier L

更新日期：2004-09-01 00:00:00

abstract：:Aptamers are short single-stranded nucleic acid molecules that are selected in vitro from a large random sequence library based on their high and specific affinity to a target molecule by a process known as SELEX. Cell-SELEX that employs whole living cells overexpressing the defined cell surface proteins (for selectio...

journal_title：Biochimie

authors： Takahashi M,Sakota E,Nakamura Y

更新日期：2016-12-01 00:00:00

abstract：:This review discusses the regulation of a key controlling step in the initiation of protein synthesis, the binding of mRNA to ribosomes. Particular focus is given to the phosphorylation of the cap-binding factor, eIF-4E, and the role of this factor in the regulation of cell growth. ...

journal_title：Biochimie

authors： Sonenberg N

更新日期：1994-01-01 00:00:00

abstract：:Epigenetic mechanisms are likely to play a role in many complex diseases, the extent of which we only beginning to understand. COPD and asthma are two respiratory diseases subject to strong environmental influences depending on underlying genetic susceptibility. Epigenetic mechanisms such as DNA methylation, histone m...

journal_title：Biochimie

authors： Kabesch M,Adcock IM

更新日期：2012-11-01 00:00:00

abstract：:Prostaglandin E synthase (PGES), which converts cyclooxygenase (COX)-derived prostaglandin H(2) (PGH(2)) to PGE(2), is known to comprise a group of at least three structurally and biologically distinct enzymes. Two of them are membrane-bound and have been designated as mPGES-1 and mPGES-2. mPGES-1 is a perinuclear pro...

journal_title：Biochimie

authors： Hara S,Kamei D,Sasaki Y,Tanemoto A,Nakatani Y,Murakami M

更新日期：2010-06-01 00:00:00

abstract：:Phospholipid metabolism of 3T3 mouse fibroblasts has been studied after serum stimulation of arrested cells. The study of [32Pi] incorporation shows : a) in the case of PE and PC an early peak of incorporation in the G1 phase of the cell cycle, 6 hours after serum addition; b) in the case of PI an intense initial incr...

journal_title：Biochimie

authors： Dubois C,Rampini C

更新日期：1978-01-01 00:00:00

abstract：:Phosphorus metabolites and intracellular pH have been examined in the slime mold Dictyostelium discoideum by non-destructive 31P-NMR measurements. In a spectrum from a suspension of aerobic amoebae, the major peaks are inorganic phosphate, nucleotide di- and triphosphates. In the corresponding perchloric acid extract,...

journal_title：Biochimie

authors： Satre M,Klein G,Martin JB

更新日期：1986-12-01 00:00:00

abstract：:The Cystic Fibrosis Transmembrane Regulator (CFTR) is a membrane protein whose mutations cause cystic fibrosis, a lethal genetic disease. We performed a molecular dynamic (MD) study of the properties of the nucleotide binding domains (NBD) whose conformational changes, upon ATP binding, are the direct responsible of t...

journal_title：Biochimie

authors： Belmonte L,Moran O

更新日期：2015-04-01 00:00:00

abstract：:In most cases, anti-protein aptamers are selected by systematic evolution of ligands by exponential enrichment (SELEX) using purified recombinant protein targets. Cell surface proteins, however, are not easy targets for SELEX due to the difficulties associated with their purification. Here, we developed a novel SELEX ...

journal_title：Biochimie

authors： Ohuchi SP,Ohtsu T,Nakamura Y

更新日期：2006-07-01 00:00:00

abstract：:This paper reviews recent data on the adenylate cyclase system of the yeast Saccharomyces cerevisiae. Since the discovery of yeast adenylate cyclase mutants and the possibility of molecular biological analysis, adenylate cyclase and the subsequent steps in the cAMP cascade have become subject of intense investigation....

journal_title：Biochimie

authors： Jacquet M,Camonis J

更新日期：1985-01-01 00:00:00

abstract：:In the present study we evaluated the effect of chronic methionine administration on oxidative stress and biochemical parameters in liver and serum of rats, respectively. We also performed histological analysis in liver. Results showed that hypermethioninemia increased chemiluminescence, carbonyl content and glutathio...

journal_title：Biochimie

authors： Stefanello FM,Matté C,Pederzolli CD,Kolling J,Mescka CP,Lamers ML,de Assis AM,Perry ML,dos Santos MF,Dutra-Filho CS,Wyse AT

更新日期：2009-08-01 00:00:00

abstract：:Mouse brain myelin consists of a continuum of particles of different densities, as shown by sucrose density gradient centrifugation. In normal animals most of the material (65 per cent) is concentrated between 0.6 and 0.7 M sucrose (the maximum being found at 0.66 M sucrose, corresponding to 23 per cent). The density ...

journal_title：Biochimie

authors： Bourre JM,Pollet S,Daudu O,Le Saux F,Baumann N

更新日期：1977-01-01 00:00:00

abstract：:TP53 is mutated in most types of human cancers and is one of the most popular genes in cancer research. The p53 protein is a sensor of multiple forms of genotoxic, oncogenic and non-genotoxic stress. It suppresses growth and controls survival of stressed cells, and as such, is the focal point of selection pressures in...

journal_title：Biochimie

authors： Guimaraes DP,Hainaut P

更新日期：2002-01-01 00:00:00

abstract：:We have determined the DNA sequence of a 1-kilobase segment of the Escherichia coli chromosome. The segment contains glyW, a duplicate gene for tRNA3Gly, and its flanking regions. An insertion sequence, previously known to have occurred spontaneously within the sequenced fragment, was identified as IS1. Possible sites...

journal_title：Biochimie

authors： Tucker SD,Murgola EJ

更新日期：1985-09-01 00:00:00

abstract：:Staphylococcus aureus is one of the most dreaded pathogens worldwide and emergence of notorious antibiotic resistant strains have further exacerbated the present scenario. The glycolytic enzyme, triosephosphate isomerase (TIM) is one of the cell envelope proteins of the coccus and is involved in biofilm formation. It ...

journal_title：Biochimie

authors： Mukherjee S,Roychowdhury A,Dutta D,Das AK

更新日期：2012-12-01 00:00:00

abstract：:alpha-Latrotoxin, a protein toxin present in the venom of black widow spider, interacts with membrane receptors of neurons and other secretory cells to stimulate exocytosis. Two types of receptors have been identified and cloned. Our attention has been focused on the calcium independent receptor, a G-protein coupled r...

journal_title：Biochimie

authors： Pescatori M,Grasso A

更新日期：2000-09-01 00:00:00

abstract：:The redox homeostasis of cytoplasm is maintained by a series of disulfide exchange reactions mediated by proteins belonging to the thioredoxin superfamily. Thioredoxin and thioredoxin reductase, being the major members of the family, play a key role in oxidative stress response of Staphylococcus aureus. In this report...

journal_title：Biochimie

authors： Bose M,Bhattacharyya S,Biswas R,Roychowdhury A,Bhattacharjee A,Ghosh AK,Das AK

更新日期：2019-05-01 00:00:00

abstract：:Bacterial survive and respond to adverse changes in the environment by regulating gene transcription through two-component regulatory systems. In Salmonella Typhimurium the STM1485 gene expression is induced under low pH (4.5) during replication inside the epithelial host cell, but it is not involved in sensing or res...

journal_title：Biochimie

authors： Torrez Lamberti MF,Farizano JV,López FE,Martínez Zamora MG,Pescaretti MM,Delgado MA

更新日期：2019-05-01 00:00:00

abstract：:Replication protein A (RPA) is a heterotrimeric single-stranded DNA binding protein whose role in DNA replication, recombination and repair has been mainly elucidated through in vitro biochemical studies utilizing the mammalian complex. However, the identification of homologs of all three subunits in Saccharomyces cer...

journal_title：Biochimie

authors： Smith J,Zou H,Rothstein R

更新日期：2000-01-01 00:00:00

abstract：:Cryptogein, a 98 amino acid protein secreted by the fungus Phytophthora cryptogea, induces a hypersensitive response and systemic acquired resistance in tobacco plants (Nicotiana tabacum var Xanthi). The mode of action of cryptogein has been studied using tobacco cell suspensions. The recognition of this elicitor by a...

journal_title：Biochimie

authors： Lebrun-Garcia A,Bourque S,Binet MN,Ouaked F,Wendehenne D,Chiltz A,Schäffner A,Pugin A

更新日期：1999-06-01 00:00:00

abstract：:The two subunits of the nickel-iron hydrogenase from Desulfovibrio gigas have been purified by preparative sodium dodecyl sulfate polyacrylamide gel electrophoresis and their amino acid compositions have been determined. The N-terminal sequences for 15 residues of the large subunit (Mr 62,000) and 25 residues of the s...

journal_title：Biochimie

authors： Nivière V,Forget N,Bovier-Lapierre G,Bonicel J,Hatchikian C

更新日期：1988-02-01 00:00:00

abstract：:Sphingolipids are degraded by sphingomyelinase and ceramidase in the gut to ceramide and sphingosine, which may inhibit cell proliferation and induce apoptosis, and thus have anti-tumour effects in the gut. Although previous rodent studies including experiments on knockout mice indicate a role of neutral ceramidase in...

journal_title：Biochimie

authors： Ohlsson L,Palmberg C,Duan RD,Olsson M,Bergman T,Nilsson A

更新日期：2007-08-01 00:00:00

abstract：:Datura stramonium contains a compound that impairs learning retention in mice. It has been purified to homogeneity and its structure has been established as that of a gamma-L-glutamyl-L-aspartate. The biological activity of this pseudodipeptide has been found to be identical with that of the corresponding synthetic on...

journal_title：Biochimie

authors： Schmitz-Bourgeois M,Amiri I,Reinbolt J,Boulanger Y,Ungerer A

更新日期：1988-09-01 00:00:00

abstract：:During the suckling period, the rats are fed continuously with milk, which is a high-fat low-carbohydrate diet (HF). At weaning, milk is progressively replaced by the rat's laboratory chow which is a high-carbohydrate low-fat diet (HCHO), and this is accompanied by large hormonal modifications: an increase in plasma i...

journal_title：Biochimie

authors： Girard J,Perdereau D,Narkewicz M,Coupé C,Ferré P,Decaux JF,Bossard P

更新日期：1991-01-01 00:00:00

abstract：:In this contribution, we have studied the dynamics of electron transfer (ET) of a flavoprotein to the bound cofactor, an important metabolic process, in a model molecular/macromolecular crowding environments. Vitamin B(2) (riboflavin, Rf) and riboflavin binding protein (RBP) are used as model cofactor and flavoprotein...

journal_title：Biochimie

authors： Rakshit S,Saha R,Verma PK,Mitra RK,Pal SK

更新日期：2012-12-01 00:00:00