Abstract:
:Kex2 is a Ca2+-dependent serine protease from S. cerevisiae. Characterization of the substrate specificity of Kex2 is of particular interest because this protease serves as the prototype of a large family of eukaryotic subtilisin-related proprotein-processing proteases that cleave sites consisting of pairs or clusters of basic residues. Our goal was to study the prime region subsite S' of Kex2 because previous studies have only taken into account non-prime sites using AMC substrates but not the specificity of prime sites identified through structural modeling or predicted cleavage sites. Therefore, we used peptides derived from Abz-KR↓EADQ-EDDnp and Abz-YKR↓EADQ-EDDnp based on the pro-α-mating factor sequence. The specificity of Kex2 due to basic residues at P1' is affected by the type of residue in the P3 position. Some residues in P1' with large or bulky side chains yielded poor substrate specificity. The kcat/KM values for peptides with P2' substitutions containing Tyr in P3 were higher than those obtained for the peptides without Tyr. In fact, P' and P modifications mainly promoted changes in kcat and KM, respectively. The pH profile of Kex2 was fit to a double-sigmoidal pH-titration curve. The specificity results suggest that Kex2 might be involved in the processing of the putative cleavage sites in a polypeptide involved in cell elongation, hyphal formation and the processing of a toxin, which result in host cell lysis. In summary, the specificity of Kex2 is dependent on the set of interactions with prime and non-prime subsites, resulting in synergism.
journal_name
Biochimiejournal_title
Biochimieauthors
Manfredi MA,Antunes AA,Jesus LO,Juliano MA,Juliano L,Judice WAdoi
10.1016/j.biochi.2016.10.003subject
Has Abstractpub_date
2016-12-01 00:00:00pages
149-158eissn
0300-9084issn
1638-6183pii
S0300-9084(16)30235-8journal_volume
131pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract::The crystallographic structure of a novel trypsin inhibitor (CTI) from Copaifera langsdorffii is reported. The structure was solved by MIRAS procedure and refined to a crystallographic residual of 17.3% (R(free) = 20.3%) at 1.8 A resolution. Two isomorphous derivatives were obtained by quick cryo-soaking approach. CTI...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2004.03.004
更新日期:2004-03-01 00:00:00
abstract::Matrix metalloproteinases (MMPs) are a family of zinc endopeptidases that process growth factors, growth factor binding proteins, cell surface proteins, degrade extracellular matrix (ECM) components and thereby play a central role in tissue remodeling and tumor progression. Membrane-type matrix metalloproteinases (MT-...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2004.07.012
更新日期:2005-03-01 00:00:00
abstract::We have synthesized and examined a number of fully and partly self-complementary palindromic oligodeoxynucleotides for their ability to assume in solution a unimolecular hairpin structure. The main results obtained by a combined optical and electrophoresis investigation show that: (i) DNA folding needs not be driven b...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(89)90042-4
更新日期:1989-07-01 00:00:00
abstract::The functions of residues 57-RY-58, G60, L77, 80-GSGR-83, I101, T104, 134-GY-135, N155, V157 and 160-LV-161 in human arsenic (III) methyltransferase (hAS3MT) 5 Å around S-adenosylmethionine (SAM) have not been studied. Herein, sixteen mutants were designed by substituting these residues with Ala. Mutants G60A, G80A, I...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2014.10.014
更新日期:2014-12-01 00:00:00
abstract::The Rap family of small GTP-binding proteins is composed by four different members: Rap1A, Rap1B, Rap2A and Rap2B. In this work we report the identification and characterization of a fifth member of this family of small GTPases. This new protein is highly homologous to Rap2A and Rap2B, binds labeled GTP on nitrocellul...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2005.08.007
更新日期:2006-03-01 00:00:00
abstract::pH-Dependence of hydrolytic activity of trypsin has been studied in cationic reverse micellar system of cetyltrimethylammonium bromide (CTAB) in (50% v/v) chloroform/isooctane using a positively charged substrate N(alpha)-benzoyl-L-arginine ethyl ester (BAEE). The pH of the medium was varied from 4.0 to 8.5 with addit...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(99)80007-8
更新日期:1998-12-01 00:00:00
abstract::Here we report two novel 17-mer amidated linear peptides (TsAP-1 and TsAP-2) whose structures were deduced from cDNAs cloned from a venom-derived cDNA library of the Brazilian yellow scorpion, Tityus serrulatus. Both mature peptides were structurally-characterised following their location in chromatographic fractions ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2013.06.003
更新日期:2013-09-01 00:00:00
abstract::Low bone formation in osteoporosis is associated with a shift from osteoblastic to adipogenic differentiation of mesenchymal stem cells (MSC) inducing a concomitant lipotoxic milieu within the bone marrow. Strontium ranelate (SrRN), a treatment for osteoporosis, has both anti-resorptive and anabolic effects on bone. T...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2012.11.008
更新日期:2013-04-01 00:00:00
abstract::During the suckling period, the rats are fed continuously with milk, which is a high-fat low-carbohydrate diet (HF). At weaning, milk is progressively replaced by the rat's laboratory chow which is a high-carbohydrate low-fat diet (HCHO), and this is accompanied by large hormonal modifications: an increase in plasma i...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(91)90077-e
更新日期:1991-01-01 00:00:00
abstract::The human liver fatty acid binding protein (hFABP1) participates in cellular long-chain fatty acid trafficking and regulation of lipid metabolism and changes in hFABP1 are associated with an increased risk for type 2 diabetes, cardiovascular disease (CVD), and metabolic syndromes. Gene regulation of hFABP1 is not full...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2011.08.006
更新日期:2012-02-01 00:00:00
abstract::The wheat germ translation initiation factor 2 (WGeIF-2) was isolated in a homogeneous state by an efficient procedure and characterized. Its molecular mass, as determined by a gel-filtration method is approximately 150,000 Da. According to SDS-PAGE WGeIF-2 consists of four subunits with M(r) 37,000 (alpha), 40,000 (b...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(92)90085-s
更新日期:1992-05-01 00:00:00
abstract::Our study highlights the tight relationship between protein binding to monolayers and the phase-state of the phospholipids. Interaction of mitochondrial creatine kinase with phospholipidic membranes was analysed using a two-phase monolayer system containing anionic phospholipids under chain mismatch conditions. Monola...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2009.03.014
更新日期:2009-06-01 00:00:00
abstract::Interferons-alpha (IFN-alpha) are pleiotropic cytokines belonging to type I IFNs, extensively used in the treatment of patients with some types of cancer and viral disease. IFN-alpha can affect tumor cell functions by multiple mechanisms. In addition, these cytokines can promote the differentiation and activity of hos...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2007.04.006
更新日期:2007-06-01 00:00:00
abstract::We identified a T-to-C mutation 2 nucleotides (nt) upstream from the AG in a GT-AG intron between exons 2 and 3 in the human T-cell leukemia virus type I (HTLV-I) tax mRNA. This mutation resulted in the preferential usage of an alternative splice site, causing a 75-nt elongation of tax mRNA and reduced production of v...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2005.07.013
更新日期:2006-02-01 00:00:00
abstract::Two forms of baker's yease valyl-tRNA synthetase have been purified to apparent homogeneity by classical methods. It was demonstrated that one of the two forms of the enzyme originates from the other by proteolysis, the respective amounts of each form depending on the physiological state of the yeast. The species main...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(76)80579-2
更新日期:1975-01-01 00:00:00
abstract::Human serum was submitted to a one step displacement-ligand exchange chromatography. Displacement removed serum albumin and part of gamma-globulins. Ligand exchange furnished an enriched heme-hemopexin fraction. An original, non denaturing human heme-hemopexin preparation is proposed. ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(78)80750-0
更新日期:1978-01-01 00:00:00
abstract::The rat and rabbit lung isoform pattern before (fetal) and after (adult) lung maturation has been analyzed by isoelectric focusing. This pattern has been compared to that of brain tubulin at the same developmental stages. No changes in the pattern of fetal and adult lung tubulin were found. However an increase in brai...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(85)80301-1
更新日期:1985-09-01 00:00:00
abstract::Human mitochondrial aminoacyl-tRNA synthetases (mt-aaRSs), the enzymes which esterify tRNAs with the cognate specific amino acid, form mainly a different set of proteins than those involved in the cytosolic translation machinery. Many of the mt-aaRSs are of bacterial-type in regard of sequence and modular structural o...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2009.02.005
更新日期:2009-05-01 00:00:00
abstract::Epidemiological studies have demonstrated that the diabetes mellitus is a serious health burden for both governments and healthcare providers. The present study was hypothesized to evaluate the antihyperglycemic potential of fraxetin by determining the activities of key enzymes of carbohydrate metabolism in streptozot...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2013.06.013
更新日期:2013-10-01 00:00:00
abstract::RNA phosphodiester bonds can be cleaved by metal ions, of which Pb2+ is one of the most effective. It can cleave both generally and site-specifically, depending on the substrate and the conditions. In addition, metal ions are also known to cleave ester bonds between amino acid and the 3'-end of transfer RNA. Here we r...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(94)90058-2
更新日期:1994-01-01 00:00:00
abstract::Three different hydrogenases (isolated from Clostridium pasteurianum, Desulfovibrio desulfuricans strain Norway 4 and D. baculatus 9974) added to a suspension of TiO2 (anatase) powder are able to catalyze H2 evolution under band gap illumination of the semiconducting particles, and in the presence of EDTA or methanol ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(86)81086-0
更新日期:1986-01-01 00:00:00
abstract::Inducing brown adipocytes in white adipose tissues is a promising target to combat obesity and its related disorders in human beings. This goal has been especially encouraged by new important discoveries of human brown adipose tissues. The accumulating evidence confirms the presence of active brown adipocytes, not onl...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2017.04.017
更新日期:2017-07-01 00:00:00
abstract::Betaine aldehyde dehydrogenase from the human opportunistic pathogen Pseudomonas aeruginosa (PaBADH) catalyzes the irreversible, NAD(P)(+)-dependent oxidation of betaine aldehyde, producing glycine betaine, an osmoprotectant. PaBADH participates in the catabolism of choline and likely in the defense against the osmoti...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2010.09.022
更新日期:2011-02-01 00:00:00
abstract::Two soluble forms of brain glutamate dehydrogenase isoproteins were inactivated by pyridoxal 5'-phosphate. Restoration of catalytic activity can be accomplished by dialysis and addition of an excess of cysteine or lysine. Spectral evidence is presented to indicate that the inactivation proceeds through Schiff base for...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(97)84333-7
更新日期:1996-01-01 00:00:00
abstract::At least 6 N-acetylglucosaminyltransferases (GlcNAc-T I, II, III, IV, V and VI) are involved in initiating the synthesis of the various branches found in complex asparagine-linked oligosaccharides (N-glycans), as indicated below: GlcNAc beta 1-6 GlcNAc-T V GlcNAc beta 1-4 GlcNAc-T VI GlcNAc beta 1-2Man alpha 1-6 GlcNA...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(88)90289-1
更新日期:1988-11-01 00:00:00
abstract::The major pterin in Tetrahymena pyriformis, strain W, earlier suggested to be L-threo-biopterin and named ciliapterin [1] is now identified as D-threo-neopterin (D-monapterin). This is the first example of a natural D-monapterin. This compound was characterized by its chromatographic behavior, its fluorescence propert...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(91)90089-j
更新日期:1991-10-01 00:00:00
abstract::Single cell oils (SCOs) are now produced by various microorganisms as commercial sources of arachidonic acid (ARA) and docosahexaenoic acid (DHA). These oils are now used extensively as dietary supplements in infant formulas. An understanding of the underlying biochemistry and genetics of oil accumulation in such micr...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2004.09.017
更新日期:2004-11-01 00:00:00
abstract::Sphingosine is a natural sphingolipid found in membranes of all eukaryotic cells. In addition to its functions in cell signaling, sphingosine has broad-spectrum antimicrobial properties. Sphingosine's role as an antimicrobial is important in tissues such as the skin and respiratory epithelium. Reduction in the normal ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2017.03.014
更新日期:2017-10-01 00:00:00
abstract::Peptides with hormonal or neuronal activity are derived by enzymatic processing from pro-hormones, which by themselves are biologically inert. Processing and other enzymatic conversions may occur step-wise, leading to the formation of a cascade of biologically active (or inactive) peptides. The neurokin in substance P...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(88)90159-9
更新日期:1988-01-01 00:00:00
abstract::Our principle hypothesis is that the hypothalamic hormone, gonadotropin-releasing hormone (GnRH), is an immunostimulatory hormone and plays a pivotal role in the gender differences in immunity and/or autoimmunity. As a general rule, females display heightened immune responses and heightened endocrinological responsive...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(03)00106-8
更新日期:2003-06-01 00:00:00