[A new protein from human plasma lipoproteins: isolation and partial characterization of apolipoprotein G].

abstract：:Histidine-proline-rich glycoprotein (HPRG), or histidine-rich glycoprotein (HRG), is a serum protein that is synthesized in the liver and is actively internalised by different cells, including skeletal muscle. The multidomain arrangement of HPRG comprises two modules at the N-terminus that are homologous to cystatin b...

journal_title：Biochimie

authors： Ronca F,Raggi A

更新日期：2015-11-01 00:00:00

abstract：:The thyroid hormones L-thyroxine and triiodo-L-thyronine have profound effects on postembryonic development of most vertebrates. Analysis of their action in mammals is vitiated by the exposure of the developing foetus to a number of maternal factors which do not allow one to specifically define the role of thyroid hor...

journal_title：Biochimie

authors： Tata JR

更新日期：1999-04-01 00:00:00

abstract：:The complete amino acid sequences of 3 proteins from the 50S subunit of Bacillus stearothermophilus ribosomes were determined by N-terminal sequence analysis and by sequencing of overlapping fragments obtained from enzymatic digestions and chemical cleavages. The proteins BstL28, BstL33 and BstL34, named according to ...

journal_title：Biochimie

authors： Kruft V,Kapp U,Wittmann-Liebold B

更新日期：1991-07-01 00:00:00

abstract：:A DNA sequence encoding a protein with predicted EF-hand and dynein light chain binding domains was identified in a Fasciola hepatica EST library. Sequence analysis of the encoded protein revealed that the most similar known protein was the Fasciola gigantica protein FgCaBP3 and so this newly identified protein was na...

journal_title：Biochimie

authors： Banford S,Drysdale O,Hoey EM,Trudgett A,Timson DJ

更新日期：2013-04-01 00:00:00

abstract：:The adaptive response appears in E. coli after exposure to low levels of alkylating agents. This system is under the positive control of the ada gene. At least two enzymes are induced during the response: 3-methyladenine DNA glycosylase II and O6-methylguanine DNA methyltransferase. The latter is also the product of t...

journal_title：Biochimie

authors： Defais M

更新日期：1985-03-01 00:00:00

abstract：:Analysis of the nucleotide sequence of 1,400 transfer RNAs has revealed the imprint of a prototypic genetic code in position 3-4-5 of the acceptor stem. It appears only in the transfer RNAs for the primordial amino acids ie those found by chemical condensation of a nitrogen-methane-water-ammonia mixture. The model for...

journal_title：Biochimie

authors： Möller W,Janssen GM

更新日期：1990-05-01 00:00:00

abstract：UNLABELLED:Pancreatic cancer (PC) is one of the most lethal human malignancies and a major health problem. Patients diagnosed with PC and treated with conventional approaches have an overall 5-year survival rate of less than 5%. Novel strategies are needed to treat this disease. Herein, we propose a combinatorial strat...

journal_title：Biochimie

authors： Breton CS,Aubry D,Ginet V,Puyal J,Heulot M,Widmann C,Duchosal MA,Nahimana A

更新日期：2015-09-01 00:00:00

abstract：:Peptides with hormonal or neuronal activity are derived by enzymatic processing from pro-hormones, which by themselves are biologically inert. Processing and other enzymatic conversions may occur step-wise, leading to the formation of a cascade of biologically active (or inactive) peptides. The neurokin in substance P...

journal_title：Biochimie

authors： Nyberg F,Le Grevés P,Terenius L

更新日期：1988-01-01 00:00:00

abstract：:The molecular basis for the essential role of cholesterol in mammalian (and other cholesterol-requiring) cells has long been the object of intense interest. Cholesterol has been found to modulate the function of membrane proteins critical to cellular function. Current literature supports two mechanisms for this modula...

journal_title：Biochimie

authors： Yeagle PL

更新日期：1991-10-01 00:00:00

abstract：:Binding of indoloquinolines with different aliphatic side chains to a parallel G-quadruplex derived from the MYC promoter sequence was characterized by optical and calorimetric measurements. ITC experiments performed at different temperatures enabled the determination of molar heat capacity changes upon quadruplex bin...

journal_title：Biochimie

authors： Funke A,Weisz K

更新日期：2019-02-01 00:00:00

abstract：:We present here, a new method for coupling sulfhydryl groups (SH) to low-density lipoprotein (LDL) surface. This method uses homocysteine thiolactone (HCTL) which reacts with lysine residues in a very mild manner, and permits the selection of the number of SH bound per LDL. Under our experimental conditions (8 SH/LDL)...

journal_title：Biochimie

authors： Vidal M,Sainte-Marie J,Philippot J,Bienvenue A

更新日期：1986-05-01 00:00:00

abstract：:A soluble hydrogenase from the halophilic sulfate reducing bacterium Desulfovibrio salexigens, strain British Guiana (NCIB 8403) has been purified to apparent homogeneity with a final specific activity of 760 mumoles H2 evolved/min/mg (an overall 180-fold purification with 20% recovery yield). The enzyme is composed o...

journal_title：Biochimie

authors： Teixeira M,Moura I,Fauque G,Czechowski M,Berlier Y,Lespinat PA,Le Gall J,Xavier AV,Moura JJ

更新日期：1986-01-01 00:00:00

abstract：:A d-carbamoylase from Sinorhizobium morelens S-5 was purified and characterized. The enzyme was purified 189-fold to homogeneity with a yield of 19.1% by aqueous two-phase extraction and two steps of column chromatography. The enzyme is a homotetramer with a native molecular mass of 150 kDa and a subunit relative mole...

journal_title：Biochimie

authors： Wu S,Liu Y,Zhao G,Wang J,Sun W

更新日期：2006-03-01 00:00:00

abstract：:The termination of protein synthesis in bacteria requires codon-specific polypeptide release factors RF-1 (UAG/UAA specific) and RF-2 (UGA/UAA specific). We have proposed that release factors mimic tRNA and recognize the stop codon for polypeptide release (Nakamura et al (1996) Cell 87, 147-150). In contrast to the te...

journal_title：Biochimie

authors： Uno M,Ito K,Nakamura Y

更新日期：1996-01-01 00:00:00

abstract：:Knowing the structure of a protein is essential to characterize its function and mechanism at the molecular level. Despite major advances in solving structures experimentally, most membrane protein native conformations remain unknown. This lack of available structures, along with the physical constraints imposed by th...

journal_title：Biochimie

authors： Postic G,Ghouzam Y,Gelly JC

更新日期：2015-08-01 00:00:00

abstract：:Bikunin (Bk) is a Künitz-type serine proteinase inhibitor, which occurs in human plasma, mainly as covalent complexes with one or two of the three peptide heavy chains. The leading member of this glycoprotein family is inter-alpha-inhibitor (I alpha I), which consists of two heavy chains (H1 and H2) linked to Bk. Bk c...

journal_title：Biochimie

authors： Capon C,Mizon C,Lemoine J,Rodié-Talbère P,Mizon J

更新日期：2003-01-01 00:00:00

abstract：:RNA-protein crosslinks were introduced into the 40S ribosomal subunits from Saccharomyces cerevisiae by mild UV treatment. Proteins crosslinked to the 18S rRNA molecule were separated from free proteins by repeated extraction of the treated subunits and centrifugation in glycerol gradients. After digestion with RNase ...

journal_title：Biochimie

authors： Xiang RH,Lee JC

更新日期：1989-11-01 00:00:00

abstract：:Changes in the cytosolic Ca(2+) concentration ([Ca(2+)](c)) translate a variety of extracellular signals into widely diverse intracellular effects, ranging from secretion to movement, proliferation and also cell death. As regards the last one, it has long been known that large [Ca(2+)](c) increases lead cells to death...

journal_title：Biochimie

authors： Pinton P,Ferrari D,Rapizzi E,Di Virgilio F,Pozzan T,Rizzuto R

更新日期：2002-02-01 00:00:00

abstract：:A major tolerance mechanism that functions to replicate damaged genomic DNA across lesions that have escaped elimination by repair mechanism is translesion DNA synthesis (TLS). DNA polymerase kappa (Pol kappa), a specialised low-fidelity DNA polymerase which is able to perform DNA synthesis across several damaged base...

journal_title：Biochimie

authors： Bavoux C,Hoffmann JS,Cazaux C

更新日期：2005-07-01 00:00:00

abstract：:Hyper- (450 mOsm/l) and hypoosmotic exposure (150 mOsm/l) of Caco-2 cells, a human intestinal epithelial cell line, induced a twofold- and a fivefold increase in the production of IL-8, a constitutively expressed cytokine, respectively. This was observed both in the presence or in the absence of added proinflammatory ...

journal_title：Biochimie

authors： Hubert A,Cauliez B,Chedeville A,Husson A,Lavoinne A

更新日期：2004-08-01 00:00:00

abstract：:The systems involving folate and cobalamin have several features in common: 1) their dietary forms require luminal digestion for absorption; 2) intestinal bacteria in the upper intestine synthesize and utilize both vitamins, creating possible competition for the nutrients; 3) there is one major intestinal brush border...

journal_title：Biochimie

authors： Alpers DH

更新日期：2016-07-01 00:00:00

abstract：:We have produced and purified an active site mutant of the Escherichia coli cyclopropane fatty acid synthase (CFAS) by replacing the strictly conserved G236 within cyclopropane synthases, by a glutamate residue, which corresponds to E146 of the homologous mycolic acid methyltransferase, Hma, producing hydroxymethyl my...

journal_title：Biochimie

authors： E G,Drujon T,Correia I,Ploux O,Guianvarc'h D

更新日期：2013-12-01 00:00:00

abstract：:A human replication initiation protein, Cdt1, is a central player in the cell cycle regulation of DNA replication, and geminin down-regulates Cdt1 function by direct binding. It has been demonstrated that Cdt1 hyperfunction resulting from Cdt1-geminin imbalance, for example, by geminin silencing with small interfering...

journal_title：Biochimie

authors： Mizushina Y,Takeuchi T,Hada T,Maeda N,Sugawara F,Yoshida H,Fujita M

更新日期：2008-06-01 00:00:00

abstract：:Flavodoxins are one domain alpha/beta electron transfer proteins that participate in photosynthetic reactions. All flavodoxins carry a molecule of flavin mononucleotide (FMN), non-covalently bound, that confers redox properties to the protein. There are two structurally distinct flavodoxins, short ones and long flavod...

journal_title：Biochimie

authors： Maldonado S,Lostao A,Irún MP,Férnandez-Recio J,Gustavo Genzor C,Begoña González E,Rubio JA,Luquita A,Daoudi F,Sancho J

更新日期：1998-10-01 00:00:00

abstract：:Extracellular Hsp70 (eHsp70) can activate immune cells via Toll-like receptors (TLR) 2 and 4, and induce cytokine synthesis. The aim of this study was to explore inflammation-associated effects of eHsp70 alone and in combination with cigarette smoke extract (CSE) in primary bronchial epithelial cells. We assessed IL-6...

journal_title：Biochimie

authors： Hulina-Tomašković A,Heijink IH,Jonker MR,Somborac-Bačura A,Grdić Rajković M,Rumora L

更新日期：2019-01-01 00:00:00

abstract：:Present knowledge of the different enzymatic steps of the denitrification chains in various bacteria, particularly Paracoccus denitrificans and Pseudomonas aeruginosa has been briefly reviewed. The question whether nitric oxide (NO), nitrous oxide (N2O) and other nitrogen derivatives are obligatory intermediates has b...

journal_title：Biochimie

authors： Henry Y,Bessières P

更新日期：1984-04-01 00:00:00

abstract：:We have modified elongation factor Tu (EF-Tu) from Escherichia coli via mutagenesis of its encoding tufA gene to study its function-structure relationships. The isolation of the N-terminal half molecule of EF-Tu (G domain) has facilitated the analysis of the basic EF-Tu activities, since the G domain binds the substra...

journal_title：Biochimie

authors： Anborgh PH,Cool RH,Gümüsel F,Harmark K,Jacquet E,Weijland A,Mistou MY,Parmeggiani A

更新日期：1991-07-01 00:00:00

abstract：:Mammalian chymotrypsin-like serine proteases (SPs) are one of the best-studied family of enzymes with roles in a wide range of physiological processes, including digestion, blood coagulation, fibrinolysis and humoral immunity. Extracellular SPs can form cascades, in which one protease activates the zymogen of the next...

journal_title：Biochimie

authors： Veillard F,Troxler L,Reichhart JM

更新日期：2016-03-01 00:00:00

abstract：:Zika and Dengue viruses have attracted substantial attention from researchers in light of recent outbreaks of Dengue fever and increases in cases of congenital microcephaly in areas with Zika incidence. This review summarizes the current state of knowledge about Zika and Dengue proteases. These enzymes have several in...

journal_title：Biochimie

authors： Majerová T,Novotný P,Krýsová E,Konvalinka J

更新日期：2019-11-01 00:00:00

abstract：:Accumulating evidences suggest that Herba epimedii has the potential benefits against osteoporosis. However, previous studies were focused on the crude extract, total flavonoids (TF) and icariin (ICA), and the detailed molecular mechanisms of action and structure-activity relationship (SAR) remain unclear. Herein we a...

journal_title：Biochimie

authors： Zhang D,Zhang J,Fong C,Yao X,Yang M

更新日期：2012-12-01 00:00:00