Abstract：
:RNA-protein crosslinks were introduced into the 40S ribosomal subunits from Saccharomyces cerevisiae by mild UV treatment. Proteins crosslinked to the 18S rRNA molecule were separated from free proteins by repeated extraction of the treated subunits and centrifugation in glycerol gradients. After digestion with RNase to remove the RNA molecules, proteins were radio-labeled with 125I and identified by electrophoresis on two-dimensional polyacrylamide gels with carrier total 40S ribosomal proteins and autoradiography. Proteins S2, S7, S13, S14, S17/22/27, and S18 were linked to the 18S rRNA. A shorter period of irradiation resulted in crosslinking of S2 and S17/22/27 only. Several of these proteins were previously demonstrated to be present in ribosomal core particles or early assembled proteins.
journal_name
Biochimiejournal_title
Biochimieauthors
Xiang RH,Lee JCdoi
10.1016/0300-9084(89)90024-2subject
Has Abstractpub_date
1989-11-01 00:00:00pages
1201-4issue
11-12eissn
0300-9084issn
1638-6183pii
0300-9084(89)90024-2journal_volume
71pub_type
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