Abstract:
:In vertebrates, mannose 6-phosphate receptors [MPR300 (Mr 300 kDa) and MPR46 (Mr 46 kDa)] are highly conserved transmembrane glycoproteins that mediate transport of lysosomal enzymes to lysosomes. Our studies have revealed the appearance of these putative receptors in invertebrates such as the molluscs and deuterostomes. Starfish tissue extracts contain several lysosomal enzyme activities and here we describe the affinity purification of α-fucosidase. The purified enzyme is a glycoprotein that exhibited a molecular mass of ∼56 kDa in SDS-PAGE under reducing conditions. It has also cross-reacted with an antiserum to the mollusc enzyme suggesting antigenic similarities among the two invertebrate enzymes. LC-MS/MS analysis of the proteolytic peptides of the purified enzyme in combination with de novo sequencing allowed us to do partial amino acid sequence determination of the enzyme. These data suggest that this invertebrate enzyme is homologous to the known mammalian enzyme. The purified enzyme exhibited a mannose 6-phosphate dependent interaction with the immobilized starfish MPR300 protein. Our results demonstrate that the lysosomal enzyme targeting pathway is conserved even among the invertebrates.
journal_name
Biochimiejournal_title
Biochimieauthors
Visa M,Hammer E,Völker U,Koliwer-Brandl H,Kelm S,Nadimpalli SKdoi
10.1016/j.biochi.2012.02.007subject
Has Abstractpub_date
2012-05-01 00:00:00pages
1199-205issue
5eissn
0300-9084issn
1638-6183pii
S0300-9084(12)00048-Xjournal_volume
94pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract::Phospholipid analysis of the membranes associated with fast sedimenting folded chromosomes prepared by lysis of E. coli CR 34 shows that both inner and outer membranes are parts of the complex, in proportions not very different from that found in the whole bacteria. During the preparation of the folded chromosomes, th...
journal_title:Biochimie
pub_type: 杂志文章
doi:
更新日期:1977-01-01 00:00:00
abstract::Using a rapid FPLC procedure for the separation of calpain I, calpain II and the inhibitor calpastatin from pork meat, results of proteolytic and inhibitory activity were obtained. Duplicate measurements showed variation coefficients less than 0.13. Compared to others we found lower calpain I activity 1 h post-mortem....
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(93)90041-p
更新日期:1993-01-01 00:00:00
abstract::RNA phosphodiester bonds can be cleaved by metal ions, of which Pb2+ is one of the most effective. It can cleave both generally and site-specifically, depending on the substrate and the conditions. In addition, metal ions are also known to cleave ester bonds between amino acid and the 3'-end of transfer RNA. Here we r...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(94)90058-2
更新日期:1994-01-01 00:00:00
abstract::Estradiol-PAB-cellulose, an easily prepared adsorbent, has been proposed to purify the uterine estradiol receptor according to the principle of biospecific affinity chromatography. It apparently removes all hormone binding sites when cytosol preparations are incubated with it. A systematic study of this adsorbent was ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(76)80317-3
更新日期:1976-01-01 00:00:00
abstract::To elucidate the metabolic function of mRNA polyadenylation in Escherichia coli. we searched for a polyadenylate-binding protein as a potential mediator of the function of the poly(A) moiety. Using a nitrocellulose filter-binding assay and a Northwestern blot technique, a protein in the ribosomal supernatant fraction ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(97)82741-1
更新日期:1997-09-01 00:00:00
abstract::The gene encoding the enzyme tyrosyl tRNA synthetase from Bacillus stearothermophilus has been systematically altered using synthetic oligonucleotides as mutagens. The construction of mutations has been facilitated by using strains of bacteria defective in mismatch repair and also by utilising a genetic marker in the ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(85)80161-9
更新日期:1985-07-01 00:00:00
abstract::The metabolism of the phospholipids of the submaxillary gland are studied after intraperitoneal injections of [32P]-orthophosphate in several groups of rats. The gland contains mainly phosphatidylcholines and phosphatidylethanolamines and in smaller quantities phosphatidylinositols, phosphatidylserines, sphingomyelins...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(76)80443-9
更新日期:1976-01-01 00:00:00
abstract::The poisonous bite by Hippasa partita, a funnel web spider from the Indian subcontinent has been demonstrated to give rise to severe dermo- and myonecrosis. In this work a hemorrhagic metalloprotease, Partitagin was purified from H. partita venom by successive chromatography on Sephadex G-100, DEAE Sephadex A-50 and B...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2007.04.005
更新日期:2007-11-01 00:00:00
abstract::A human replication initiation protein, Cdt1, is a central player in the cell cycle regulation of DNA replication, and geminin down-regulates Cdt1 function by direct binding. It has been demonstrated that Cdt1 hyperfunction resulting from Cdt1-geminin imbalance, for example, by geminin silencing with small interfering...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2008.02.018
更新日期:2008-06-01 00:00:00
abstract::The targets of in vivo studies of the ribosomal assembly process are mainly the events of rRNA processing, whereas in vitro studies (total reconstitution) focus on principles of the assembly process such as assembly-initiation proteins, rate-limiting steps and a detailed sequence of assembly reactions (assembly map). ...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(91)90054-5
更新日期:1991-06-01 00:00:00
abstract::Haloalkane dehalogenases are known as bacterial enzymes cleaving a carbon-halogen bond in halogenated compounds. Here we report the first biochemically characterized non-microbial haloalkane dehalogenase DspA from Strongylocentrotus purpuratus. The enzyme shows a preference for terminally brominated hydrocarbons and e...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2013.07.025
更新日期:2013-11-01 00:00:00
abstract::The chemical carcinogen N-acetoxy-N-2-acetylaminofluorene (N-AcO-AAF) induces frameshift mutations located within two types of specific sequences (mutation hot spots): i) contiguous guanine sequences and ii) alternating GC sequences. The genetic requirements of these frameshift events were investigated using specific ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(91)90117-j
更新日期:1991-04-01 00:00:00
abstract::Ceramidase plays an important role in regulating the metabolism of sphingolipids, such as ceramide, sphingosine (SPH), and sphingosine-1-phosphate (S1P), by controlling the hydrolysis of ceramide. Here we report the cloning and biochemical characterization of a neutral ceramidase from the red flour beetle Tribolium ca...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2011.03.009
更新日期:2011-07-01 00:00:00
abstract::A set of sequences can be defined by their common subsequences, and the length of these is a measure of the overall resemblance of the set. Each subsequence corresponds to a succession of symbols embedded in every sequence, following the same order but not necessarily contiguous. Determining the longest common subsequ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(85)80271-6
更新日期:1985-05-01 00:00:00
abstract::Herein, we report the purification and biochemical characterization of a novel bi-functional protein proteinase/amylase inhibitor from the dietary leguminous pulse Phaseolus aureus Roxb. (Vigna radiata L.) by means of acetic acid precipitation, salt fractionation, ion-exchange chromatography (DEAE-cellulose) and affin...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2005.05.007
更新日期:2005-12-01 00:00:00
abstract::Isovaleryl-CoA dehydrogenase (IVD) catalyzes the conversion of isovaleryl-CoA to 3-methylcrotonyl-CoA and the transfer of electrons to the electron transfer flavoprotein (ETF). Recombinant human IVD purifies with bound CoA-persulfide. A modified purification protocol was developed to isolate IVD without bound CoA-pers...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2014.11.007
更新日期:2015-01-01 00:00:00
abstract::In the intestine, NF-κB is the main transcription factor involved in the anti-inflammatory effect of glutamine and we previously demonstrated that glutamine via its conversion to glutamate diminished the p65 protein content in Caco-2/TC7 cell nuclei without affecting the stimulating effect of IL-1β on NF-κB [21]. Howe...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2011.11.012
更新日期:2012-03-01 00:00:00
abstract::Ferritins are ubiquitous iron-storage proteins found in all kingdoms of life. They share a common architecture made of 24 subunits of five α-helices. The recombinant Chlorobium tepidum ferritin (rCtFtn) is a structurally interesting protein since sequence alignments with other ferritins show that this protein has a si...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2014.07.019
更新日期:2014-11-01 00:00:00
abstract::Glycolipids constitute a class of molecules with various biological activities. Among them, sugar-based bolaamphiphiles characterized by their biocompatibility, biodegradability and lower toxicity, became interesting for the development of efficient and low cost lipid-based drug delivery systems. Their activity seems ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2016.04.001
更新日期:2016-11-01 00:00:00
abstract::Human telomeres are intrinsically dynamic structures, with multiple biological processes operating to generate substantial length heterogeneity. Processes that operate specifically at the terminus, that include the end-replication problem coupled with C-strand resection, result in gradual telomere erosion with ongoing...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2007.08.003
更新日期:2008-01-01 00:00:00
abstract::The presence of a fragment of polyA resistant to both T1 and p ribonucleases in mRNAs extracted from wing imaginal disks of an insect, Pieris brassicae, is reported. Its length was approximatively 150 nucleotides. PolyU sepharose affinity chromatography was subsequently used for purification of these polyA(+)mRNA mole...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(75)80049-6
更新日期:1975-01-01 00:00:00
abstract::Information on the subcellular localization of bacterial proteins is essential for protein function prediction, genome annotation and drug design. Here we proposed a novel approach to predict the subcellular localization of bacterial proteins by fusing features from position-specific score matrix (PSSM), Gene Ontology...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2014.06.001
更新日期:2014-09-01 00:00:00
abstract::We have studied through the late foetal period of the rat, the evolution of two enzymes involved in glycogen metabolism of the liver : UDPG glycogen synthetase (a and b forms) and branching enzyme. The activities were measured during the development of normal foetuses and of foetuses experimentally deprived of cortico...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(75)80140-4
更新日期:1975-01-01 00:00:00
abstract::1)Individual monosaccharides (uronic acids and aminosugars) have been purified following specific hydrolysis of the mucopolysaccharides from new born rat skin (hyaluronic acid, heparin + heparan sulfate, chondroitin sulfate A, B and C), after incubation with [U14C]-glucose under various conditions and for varying incu...
journal_title:Biochimie
pub_type: 杂志文章
doi:
更新日期:1975-11-01 00:00:00
abstract::Nucleic acid Aptamers are ligands that are selected by a process of molecular evolution to bind with high affinities and specificities to a specific target. Recently, an increasing number of aptamers have been selected against biomarkers expressed at the surface of human cells or infectious pathogens. This class of ta...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2012.02.025
更新日期:2012-07-01 00:00:00
abstract::At least 6 N-acetylglucosaminyltransferases (GlcNAc-T I, II, III, IV, V and VI) are involved in initiating the synthesis of the various branches found in complex asparagine-linked oligosaccharides (N-glycans), as indicated below: GlcNAc beta 1-6 GlcNAc-T V GlcNAc beta 1-4 GlcNAc-T VI GlcNAc beta 1-2Man alpha 1-6 GlcNA...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(88)90289-1
更新日期:1988-11-01 00:00:00
abstract::The number of proteins known to contain collagen-like triple helical domains is rapidly increasing. The functions of these domains are to provide molecular rods that separate spatially non-triple helical domains with varied properties and structures and to permit lateral interactions between molecules. Two-thirds of t...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(90)90071-n
更新日期:1990-06-01 00:00:00
abstract::The quest for effective anticancer therapeutics continues to be extensively pursued. Over the past century, several drugs have been developed, however, a majority of these drugs have a poor therapeutic index and increased toxicity profile. Hence, there still exists ample opportunity to discover safe and effective anti...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2020.12.024
更新日期:2021-01-06 00:00:00
abstract::The central glycolytic and oxidative pathways and the ATP-producing mechanisms differ in sane and malignant cells by their regulation and dynamics. Fast-growing, poorly-differentiated cancer cells characteristically show high aerobic glycolysis. In the same way, cholesterol biosynthesis, which occurs by normal pathway...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(92)90016-8
更新日期:1992-11-01 00:00:00
abstract::In relation to decreased metabolic sensitivity to catecholamines observed, in vitro, in brown fat of cold-acclimated rats, beta-adrenergic receptors were studied in isolated cells and in a crude membrane preparation from rat interscapular brown adipose tissue. [3H] dihydroalprenolol binding had the same characteristic...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(84)90153-6
更新日期:1984-07-01 00:00:00