Abstract:
:The innate immunity factor lactoferrin harbours two antimicrobial moieties, lactoferricin and lactoferrampin, situated in close proximity in the N1 domain of the molecule. Most likely they cooperate in many of the beneficial activities of lactoferrin. To investigate whether chimerization of both peptides forms a functional unit we designed a chimerical structure containing lactoferricin amino acids 17-30 and lactoferrampin amino acids 265-284. The bactericidal activity of this LFchimera was found to be drastically stronger than that of the constituent peptides, as was demonstrated by the need for lower dose, shorter incubation time and less ionic strength dependency. Likewise, strongly enhanced interaction with negatively charged model membranes was found for the LFchimera relative to the constituent peptides. Thus, chimerization of the two antimicrobial peptides resembling their structural orientation in the native molecule strikingly improves their biological activity.
journal_name
Biochimiejournal_title
Biochimieauthors
Bolscher JG,Adão R,Nazmi K,van den Keybus PA,van 't Hof W,Nieuw Amerongen AV,Bastos M,Veerman ECdoi
10.1016/j.biochi.2008.05.019subject
Has Abstractpub_date
2009-01-01 00:00:00pages
123-32issue
1eissn
0300-9084issn
1638-6183pii
S0300-9084(08)00187-9journal_volume
91pub_type
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