Abstract:
:Sphingolipids are degraded by sphingomyelinase and ceramidase in the gut to ceramide and sphingosine, which may inhibit cell proliferation and induce apoptosis, and thus have anti-tumour effects in the gut. Although previous rodent studies including experiments on knockout mice indicate a role of neutral ceramidase in ceramide digestion, the human enzyme has never been purified and characterized in its purified form. We here report the purification and characterization of neutral ceramidase from human ileostomy content, using octanoyl-[(14)C]sphingosine as substrate. After four chromatographic steps, a homogeneous protein band with 116kDa was obtained. MALDI mass spectrometry identified 16 peptide masses similar to human ceramidase previously cloned by El Bawab et al. [Molecular cloning and characterization of a human mitochondrial ceramidase, J. Biol. Chem. 275 (2000) 21508-21513] and Hwang et al. [Subcellular localization of human neutral ceramidase expressed in HEK293 cells, Biochem. Biophys. Res. Commun. 331 (2005) 37-42]. By RT-PCR and 5'-RACE methods, a predicted partial nucleotide sequence of neutral ceramidase was obtained from a human duodenum biopsy sample, which was homologous to that of known neutral/alkaline ceramidases. The enzyme has neutral pH optimum and catalyses both hydrolysis and formation of ceramide without distinct bile salt dependence. It is inhibited by Cu(2+) and Zn(2+) ions and by low concentrations of cholesterol. The enzyme is a glycoprotein but deglycosylation does not affect its activity. Our study indicates that neutral ceramidase is expressed in human intestine, released in the intestinal lumen and plays a major role in ceramide metabolism in the human gut.
journal_name
Biochimiejournal_title
Biochimieauthors
Ohlsson L,Palmberg C,Duan RD,Olsson M,Bergman T,Nilsson Adoi
10.1016/j.biochi.2007.03.009subject
Has Abstractpub_date
2007-08-01 00:00:00pages
950-60issue
8eissn
0300-9084issn
1638-6183pii
S0300-9084(07)00060-0journal_volume
89pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract::Honeybee-plant relationships are based on a conditioning process in which olfactory (plant aroma) and gustatory cues (mainly nectars) are closely linked, leading to a selective foraging behaviour. Among crops dependent upon entomophilous cross-pollination, the sunflower has recently undergone extensive expansion due t...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(87)90186-6
更新日期:1987-06-01 00:00:00
abstract::Hyper- (450 mOsm/l) and hypoosmotic exposure (150 mOsm/l) of Caco-2 cells, a human intestinal epithelial cell line, induced a twofold- and a fivefold increase in the production of IL-8, a constitutively expressed cytokine, respectively. This was observed both in the presence or in the absence of added proinflammatory ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2004.07.009
更新日期:2004-08-01 00:00:00
abstract::Nucleolin (NCL) is one of the most abundant non ribosomal protein of the nucleolus where it plays a central role in polymerase I transcription. NCL is also found outside of the nucleolus, in the nucleoplasm, cytoplasm as well as on the cell membrane. It acts in all cell compartments to control cellular homeostasis and...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2015.03.023
更新日期:2015-06-01 00:00:00
abstract::Epigenetic alterations are involved in every step of carcinogenesis. The development of chromatin-modifying agents (CMAs) has provided the ability to fight cancer by reversing these alterations. Currently, four CMAs have been approved for cancer treatment; two DNA demethylating agents and two deacetylase inhibitors. A...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2012.05.012
更新日期:2012-11-01 00:00:00
abstract::The cell reproduction cycle of parasitic wall-free bacteria, mycoplasma, is reviewed. DNA replication of Mycoplasma capricolum starts at a fixed site neighboring the dnaA gene and proceeds to both directions after a short arrest in one direction. The initiation frequency fits to the slow speed of replication fork and ...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(99)00209-6
更新日期:1999-08-01 00:00:00
abstract::Diabetic cardiomyopathy is related to oxidative stress and correlated with the presence of advanced glycation end products (AGEs). In a clinical setting, AGEs can be detected in patients presenting diabetic cardiomyopathy; however, the underlying mechanism has yet to be elucidated. In our previous study, AGEs increase...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2015.07.019
更新日期:2015-11-01 00:00:00
abstract::Recent work has shown that a DBF4 analog in yeast may be a target of nitrogen-containing bisphosphonates. DBF4 is an essential protein kinase required for DNA replication from primary eukaryotes to humans and appears to play a critical role in the S-phase checkpoint. It is also required for cell migration and cell sur...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2013.11.010
更新日期:2014-04-01 00:00:00
abstract::TcrX/Y is one of the twelve two component system (TCS) present in Mycobacterium tuberculosis. We have investigated the TcrX/Y interaction by in silico studies, pull down assay, radioactive phosphotransfer, surface plasmon resonance as well as crosstalk analysis of TcrY with TcrA - a non-cognate response regulator. Seq...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2009.11.009
更新日期:2010-03-01 00:00:00
abstract::A major incentive in inhibitor research is that control of limited proteolysis constitutes a valuable pharmacological tool. Protease inhibitors have proved to be successful in influencing pathogenesis in many experimental models but a breakthrough to use in human therapy has mainly been restricted to aprotinin and ang...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(91)90084-e
更新日期:1991-01-01 00:00:00
abstract::To examine the role of the C-terminal domain in the chaperone function of trigger factor (TF), a number of truncation mutants were constructed, namely: TF419, TF389, TF380, TF360, TF344, and TF251, in which the C-terminal 13, 43, 52, 72, 88 residues or the entire C-domain were deleted, respectively. Co-expression of m...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2005.11.006
更新日期:2006-06-01 00:00:00
abstract::The eukaryotic ribosomal protein S15 is a key component of the decoding site in contrast to its prokaryotic counterpart, S19p, which is located away from the mRNA binding track on the ribosome. Here, we determined the oligopeptide of S15 neighboring the A site mRNA codon on the human 80S ribosome with the use of mRNA ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2010.02.031
更新日期:2010-07-01 00:00:00
abstract::Snake venom phospholipases A2 (PLA2) share high sequence identities and a conserved structural scaffold, but show important functional differences. Only a few PLA2s have been purified and characterized from coral snake (Micrurus spp.) venoms, and their role in envenomation remains largely unknown. In this report, we d...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2017.03.008
更新日期:2017-06-01 00:00:00
abstract::Neuropeptides and hormones, in contrast to constitutive secretory proteins, are sorted to and stored in secretory granules and released upon a stimulus. During the last two decades, signals and mechanisms involved in their sorting to the regulated pathway of protein secretion have been addressed in numerous studies. T...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(00)00195-4
更新日期:2000-04-01 00:00:00
abstract::The number of available genomic sequences is growing very fast, due to the development of massive sequencing techniques. Sequence identification is needed and contributes to the assessment of gene and species evolutionary relationships. Automated bioinformatics tools are thus necessary to carry out these identificatio...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2007.08.006
更新日期:2008-04-01 00:00:00
abstract::Thraustochytrium is a marine protist producing a specific profile of nutritionally important fatty acids, including very long chain polyunsaturated fatty acids (VLCPUFAs) docosahexaenoic acid (DHA, 22:6n-3), even chain saturated fatty acids (SFAs) palmitic acid (16:0), and odd chain SFAs pentadecanoic acid (15:0). To ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2017.10.024
更新日期:2018-01-01 00:00:00
abstract::Long term cadmium (Cd) exposure in occupational and general environments may give rise to kidney dysfunction. This effect is usually considered to be the critical effect, i. e. the effect that occurs at relatively low level of exposure. The present review focused on studies of the prevalence of cadmium-related kidney ...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2009.06.014
更新日期:2009-10-01 00:00:00
abstract:BACKGROUND:Accumulating evidence suggests that microRNA-590 (miR-590) has protective effects on cardiovascular diseases, but the mechanism is unknown. Interestingly, previous studies from our laboratory and others have shown that macrophage-derived lipoprotein lipase (LPL) might accelerate atherosclerosis by promoting ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2014.08.003
更新日期:2014-11-01 00:00:00
abstract::Sticholysin II (StII) is a pore-forming toxin of biomedical interest that belongs to the actinoporin protein family. Sticholysins are currently under examination as an active immunomodulating component of a vaccinal platform against tumoral cells and as a key element of a nucleic acids delivery system to cell cytosol....
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2018.02.006
更新日期:2018-05-01 00:00:00
abstract::The gene encoding the enzyme tyrosyl tRNA synthetase from Bacillus stearothermophilus has been systematically altered using synthetic oligonucleotides as mutagens. The construction of mutations has been facilitated by using strains of bacteria defective in mismatch repair and also by utilising a genetic marker in the ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(85)80161-9
更新日期:1985-07-01 00:00:00
abstract::A novel approach to genetic sequence analysis is presented. This approach, based on compression of algorithms, has been launched simultaneously by Grumbach and Tahi, Milosavljevic and Rivals. To reduce the description of an object, a compression algorithm replaces some regularities in the description by special codes....
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(96)84763-8
更新日期:1996-01-01 00:00:00
abstract::NADH cytochrome b5 oxidoreductase (Ncb5or) protects β-cells against oxidative stress and lipotoxicity. The predominant phenotype of lean Ncb5or-null mouse is insulin-dependent diabetes due to β-cell death. This suggests the putative role of NCB5OR polymorphism in human diabetes. Therefore, we aimed to investigate the ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2013.03.004
更新日期:2013-07-01 00:00:00
abstract::Interferons (IFNs) were considered for the treatment of patients with multiple sclerosis (MS) after the demonstration, based on small studies, of the efficacy of type IFN beta in decreasing the frequency of exacerbations in relapsing-remitting multiple sclerosis when administered intrathecally, subcutaneously, or intr...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2007.03.016
更新日期:2007-06-01 00:00:00
abstract::Accumulating evidences suggest that Herba epimedii has the potential benefits against osteoporosis. However, previous studies were focused on the crude extract, total flavonoids (TF) and icariin (ICA), and the detailed molecular mechanisms of action and structure-activity relationship (SAR) remain unclear. Herein we a...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2012.06.033
更新日期:2012-12-01 00:00:00
abstract::Human mitochondrial diseases, defined as the diseases due to a mitochondrial oxidative phosphorylation defect, represent a large group of very diverse diseases with respect to phenotype and genetic causes. They present with many unsolved issues, the comprehensive analysis of which is beyond the scope of this review. W...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2013.08.012
更新日期:2014-05-01 00:00:00
abstract::Disintegrins are small non-enzymatic proteins common in the venoms of many viperid snakes. These proteins have received significant attention due to their ability to inhibit platelet aggregation and cell adhesion, making them model compounds in drug development and design investigations. The present study used a combi...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2015.07.005
更新日期:2015-09-01 00:00:00
abstract::The issue of whether histone H1 possesses specificity of binding to certain nucleotide sequences in DNA is of fundamental importance to the suggested role of the linker histone in the regulation of gene transcription. The purpose of the present study was to reinvestigate the specificity of binding of histone H1 to the...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(93)90116-a
更新日期:1993-01-01 00:00:00
abstract::Inhibition of polypeptide chain elongation with the mRNA-complementary (antisense) oligonucleotide has been realized through a RNase H independent mechanism. Nuclease resistant complementary non-natural alpha-17-mer oligonucleotide did not inhibit cell-free protein biosynthesis of beta-globin in the wheat germ system ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(92)90089-w
更新日期:1992-05-01 00:00:00
abstract::Mitochondrial membranes are essential for the good functioning of the organelle. For instance, the inner mitochondrial membrane contains the oxidative phosphorylation system that permits ATP synthesis. Phospholipids environment and especially cardiolipin are crucial for the mitochondrial energy metabolism. Indeed, car...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2012.07.009
更新日期:2013-01-01 00:00:00
abstract::Carbonic anhydrases (CAs, EC 4.2.1.1) are metalloenzymes found throughout the phylogenetic tree. The β-class carbonic anhydrases (β-CAs) are the predominating class of CAs in plants. Growing evidence underscores the importance of β-CAs in plant immunity and environmental adaptation in addition to their roles in photos...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2013.10.020
更新日期:2014-02-01 00:00:00
abstract::Reuber H 35 hepatoma cells were synchronized by transfer in a serum free medium. Growth was re-initiated by addition of serum. Under these conditions DNA synthesis exhibited a maximum after 24 hours. Chromatin non-histone proteins prepared from cells at various phases of the cell cycle were incubated with [gamma-32P] ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(79)80280-1
更新日期:1979-01-01 00:00:00