Abstract：
:Purified chromatin from pig lymph nodes has been prepared and used for the isolation of chromatin proteins and non-histine proteins. Saccharose nuclei from this tissue have been used for the preparation of nuclear phenol-soluble phosphoproteins. The isolated proteins have been compared by analytical electrophoresis in polyacrylamide gel in sodium dodecyl sulfate. Most chromatin non-histone proteins and nuclear phosphoproteins of pig lymph nodes have a molecular weight about 40 000 daltons and show a significant degree of homology of molecular range from 40 000 to 90 000 daltons. The phosphoproteins have been also analyzed by isoelectric focusing in polyacrylamide gel over the pH range of 3-10. They are distributed throughout the pI range of 5.8-9.0. The chromatin non-histone proteins and phosphoproteins are of acidic nature and contain 0,97 per cent tryptophan.
journal_name
Biochimiejournal_title
Biochimieauthors
Kilianska Z,Klyszejko-Stefanowicz Ldoi
10.1016/s0300-9084(76)80391-4subject
Has Abstractpub_date
1976-01-01 00:00:00pages
677-80issue
6eissn
0300-9084issn
1638-6183pii
S0300-9084(76)80391-4journal_volume
58pub_type
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