Up-regulation of cytosolic tryparedoxin in Amp B resistant isolates of Leishmania donovani and its interaction with cytosolic tryparedoxin peroxidase.

Abstract:

:Leishmania is a unicellular protozoan parasite which causes leishmaniasis, a neglected tropical disease. It possess a unique thiol metabolism comprising of several proteins among which, tryparedoxin (cTXN) and tryparedoxin peroxidase (cTXNPx), function in concert as oxidoreductases, utilizing trypanothione as a source of electrons to reduce the hydroperoxides produced by macrophages during infection. This detoxification pathway is unique and essential for the survival of Leishmania. Herein, we report the functional characterization of Leishmania donovani cTXN and its interaction with cTXNPx. The full length recombinant cTXN and cTXNPx proteins were purified in the native state and biochemical analysis showed that the cTXN-cTXNPx coupled system efficiently degraded hydrogen peroxide and tert-butyl hydroperoxide by transferring reducing equivalents from trypanothione. In silico investigation of the potential interaction between cTXN and cTXNPx proteins showed strong interaction of model structures with amino acids Ile109, Thr132, Glu107, Trp70, Trp39, Cys40 and His129 of Ld-cTXN and Thr54, Lys93, Arg128 and Asn152 of Ld-cTXNPx predicted to be involved in interaction. Moreover, co-purification, pull down assay and immunoprecipitation studies confirmed the interaction between Ld-cTXN and Ld-cTXNPx proteins. In addition, for the first time, we demonstrated at the translational level that Ld-cTXN protein is upregulated in Amp B resistant isolates accompanied by enhanced peroxidase activity, as compared to sensitive strains. Thus, our results show that Ld-cTXN and Ld-cTXNPx proteins acts in concert by physical interaction to form a strong peroxide stress detoxification system in Leishmania and their upregulation in Amp B resistant isolates imparts better stress tolerance, and hence fitter pathogens, as compared to sensitive strains.

journal_name

Biochimie

journal_title

Biochimie

authors

Suman SS,Equbal A,Zaidi A,Ansari MY,Singh KP,Singh K,Purkait B,Sahoo GC,Bimal S,Das P,Ali V

doi

10.1016/j.biochi.2015.12.017

subject

Has Abstract

pub_date

2016-02-01 00:00:00

pages

312-25

eissn

0300-9084

issn

1638-6183

pii

S0300-9084(15)00431-9

journal_volume

121

pub_type

杂志文章
  • Principles and equations for measuring and interpreting protein stability: From monomer to tetramer.

    abstract::The ability to measure the thermodynamic stability of proteins with precision is important for both academic and applied research. Such measurements rely on mathematical models of the protein denaturation profile, i.e. the relation between a global protein signal, corresponding to the folding states in equilibrium, an...

    journal_title:Biochimie

    pub_type: 杂志文章,评审

    doi:10.1016/j.biochi.2015.11.013

    authors: Bedouelle H

    更新日期:2016-02-01 00:00:00

  • Purification and characterization of a approximately 34 kDa antioxidant protein (beta-turmerin) from turmeric (Curcuma longa) waste grits.

    abstract::Beta-turmerin from turmeric (Curcuma longa) waste grits obtained after extraction of curcumin was purified by successive gel permeation chromatography. Homogeneity of beta-turmerin was confirmed by its movement as single band both in SDS-PAGE and as well as in native (basic) PAGE. The apparent molecular mass is approx...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2009.06.006

    authors: Smitha S,Dhananjaya BL,Dinesha R,Srinivas L

    更新日期:2009-09-01 00:00:00

  • A stochastic approach to serotonergic fibers in mental disorders.

    abstract::Virtually all brain circuits are physically embedded in a three-dimensional matrix of fibers that release 5-hydroxytryptamine (5-HT, serotonin). The density of this matrix varies across brain regions and cortical laminae, and it is altered in some mental disorders, including Major Depressive Disorder and Autism Spectr...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2018.07.014

    authors: Janušonis S,Detering N

    更新日期:2019-06-01 00:00:00

  • Automatic identification of large collections of protein-coding or rRNA sequences.

    abstract::The number of available genomic sequences is growing very fast, due to the development of massive sequencing techniques. Sequence identification is needed and contributes to the assessment of gene and species evolutionary relationships. Automated bioinformatics tools are thus necessary to carry out these identificatio...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2007.08.006

    authors: Arigon AM,Perrière G,Gouy M

    更新日期:2008-04-01 00:00:00

  • Purification and characterization of a novel and conserved TPR-domain protein that binds both Hsp90 and Hsp70 and is expressed in all developmental stages of Leishmania major.

    abstract::Heat shock proteins (Hsps) are involved in several important aspects of the cell proteostasis. Hsp90 interacts with at least a tenth of the cell proteome helping a large number of proteins to fold correctly. Hsp90 function is modulated by several co-chaperones having TPR (tetratricopeptide repeat) domains that allow f...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2020.12.017

    authors: Araujo SA,Martins GH,Quel NG,Aragão AZB,Morea EGO,Borges JC,Houry WA,Cano MIN,Ramos CHI

    更新日期:2021-01-06 00:00:00

  • Plasmodium falciparum SERA protein peptide analogues having short helical regions induce protection against malaria.

    abstract::Three Plasmodium falciparum serine repeat antigen (SERA) protein peptides were studied by NMR and structure calculations being done in 70:30 water:trifluoroethanol solution. Peptide 22834 was shown to be immunogenic and protective against malaria in Aotus monkeys, whilst native peptide 6737 and its analogue 14096 did ...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/s0300-9084(03)00136-6

    authors: Cubillos M,Alba MP,Bermúdez A,Trujillo M,Patarroyo ME

    更新日期:2003-07-01 00:00:00

  • Amino acid sequence of rat thymus histone H2B and identification of the in vitro phosphorylation sites.

    abstract::The amino acid sequence of rat thymus histone obtained in highly purified form by preparative electrophoresis, was determined. This sequence is identical to the sequence of calf thymus histone H2B. The in vitro phosphorylation of the rat histone with a cyclic AMP-dependent protein kinase isolated from rat pancreas led...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/s0300-9084(79)80313-2

    authors: Martinage A,Mangeat P,Sautière P,Marchis-Mouren G,Biserte G

    更新日期:1979-01-01 00:00:00

  • The application of nuclear magnetic resonance spectroscopy to the study of natural and model membranes.

    abstract::This review article outlines some potentials and limits of the recent application of high resolution Nuclear Magnetic Resonance technique--coupled to the Fourier transformation methods--to the study of biological membranes. Molecular arrangement and dynamical structure characters can be assessed at the level of indivi...

    journal_title:Biochimie

    pub_type: 杂志文章,评审

    doi:10.1016/s0300-9084(75)80333-6

    authors: Podo F

    更新日期:1975-01-01 00:00:00

  • Involvement of plasma membrane proteins in plant defense responses. Analysis of the cryptogein signal transduction in tobacco.

    abstract::Cryptogein, a 98 amino acid protein secreted by the fungus Phytophthora cryptogea, induces a hypersensitive response and systemic acquired resistance in tobacco plants (Nicotiana tabacum var Xanthi). The mode of action of cryptogein has been studied using tobacco cell suspensions. The recognition of this elicitor by a...

    journal_title:Biochimie

    pub_type: 杂志文章,评审

    doi:10.1016/s0300-9084(99)80123-0

    authors: Lebrun-Garcia A,Bourque S,Binet MN,Ouaked F,Wendehenne D,Chiltz A,Schäffner A,Pugin A

    更新日期:1999-06-01 00:00:00

  • A single element in the phosphoenolpyruvate carboxykinase gene mediates thiazolidinedione action specifically in adipocytes.

    abstract::Phosphoenolpyruvate carboxykinase (PEPCK) is the key enzyme in glyceroneogenesis, an important metabolic pathway that functions to restrain the release of non-esterified fatty acids (NEFAs) from adipocytes. The antidiabetic drugs known as thiazolidinediones (TZDs) are thought to achieve some of their benefits by lower...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/s0300-9084(01)01343-8

    authors: Glorian M,Duplus E,Beale EG,Scott DK,Granner DK,Forest C

    更新日期:2001-10-01 00:00:00

  • Comparison of the invasion strategies used by Salmonella cholerae-suis, Shigella flexneri and Yersinia enterocolitica to enter cultured animal cells: endosome acidification is not required for bacterial invasion or intracellular replication.

    abstract::Strains of Escherichia, Salmonella, Shigella and Yersinia actively enter eukaryotic cells. Several techniques were used to compare and contrast the invasion mechanisms of Salmonella cholerae-suis, Yersinia enterocolitica and Shigella flexneri. Three animal cell lines (CHO, HEp-2 and MDCK) were examined for susceptibil...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/0300-9084(88)90271-4

    authors: Finlay BB,Falkow S

    更新日期:1988-08-01 00:00:00

  • The influence of adenosine on intermediary metabolism of isolated hepatocytes.

    abstract::The effect of adenosine was tested on the energetic metabolism of fed rat liver cells after isolation. The cells were incubated in a buffered saline medium with glucose (5 mM) and adenosine (1 mM) for 30 minutes at 37 degrees C. This increased the concentration of the adenylic nucleotides ATP (+57 per cent, ADP (+39 p...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/s0300-9084(80)80286-0

    authors: Marchand JC,Lavoinne A,Giroz M,Matray F

    更新日期:1979-01-01 00:00:00

  • Methylation process of mammalian ribosomal precursor RNA.

    abstract::The analysis of RNA from L5178 Y cells, by electrophoreis in 1.7% polyacrylamide gel, after short time labelling with [methyl-3H] methionine, shows that the three molecular species 47S, 46S and 45S RNA from the ribosomal RNA "45S fraction" are methylated. Comparative studies of the kinetics obtained after labelling fo...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/s0300-9084(76)80544-5

    authors: Tiollais P,Auger MA,Galibert F

    更新日期:1975-11-01 00:00:00

  • Inactivation of proteases and esterases by halomethylated derivatives of dihydrocoumarins.

    abstract::A series of halomethylated derivatives of dihydrocoumarins has been found to inhibit irreversibly proteases and esterases. alpha-Chymotrypsin, subtilish, elastase are rapidly inactivated in the presence of these compounds, while trypsin, kallicrein, papain are inhibited more slowly. Esterases like acetylcholinesterase...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/s0300-9084(77)80140-5

    authors: Béchet JJ,Dupaix A,Roucous C,Bonamy AM

    更新日期:1977-01-01 00:00:00

  • Hormone-mediated inositol lipid breakdown in hepatocytes and WRK1 cells: relationship to receptor function.

    abstract::All hormones and neurotransmitters which provoke their intracellular effects by increasing the cytosolic concentration of Ca2+ in their target cells also stimulate the breakdown of inositol phospholipids. Much evidence suggests that this breakdown is intimately involved in the mechanism which couples cell-surface rece...

    journal_title:Biochimie

    pub_type: 杂志文章,评审

    doi:10.1016/s0300-9084(85)80115-2

    authors: Kirk CJ,Guillon G,Balestre MN,Creba JA,Michell RH,Jard S

    更新日期:1985-10-01 00:00:00

  • Characterisation of a mannose-binding C-type lectin from Oxyuranus scutellatus snake venom.

    abstract::C-type lectins are calcium-dependent sugar binding proteins and are distributed ubiquitously amongst vertebrate organisms. As part of a wider study on Australian snake venom components, we have identified and characterised a C-type lectin from the venom of Oxyuranus scutellatus (Australian coastal taipan) with mannose...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2010.11.006

    authors: Earl ST,Robson J,Trabi M,de Jersey J,Masci PP,Lavin MF

    更新日期:2011-03-01 00:00:00

  • Structure-function relationships in mammalian histidine-proline-rich glycoprotein.

    abstract::Histidine-proline-rich glycoprotein (HPRG), or histidine-rich glycoprotein (HRG), is a serum protein that is synthesized in the liver and is actively internalised by different cells, including skeletal muscle. The multidomain arrangement of HPRG comprises two modules at the N-terminus that are homologous to cystatin b...

    journal_title:Biochimie

    pub_type: 杂志文章,评审

    doi:10.1016/j.biochi.2015.09.024

    authors: Ronca F,Raggi A

    更新日期:2015-11-01 00:00:00

  • Running up that hill: How to create cellular lipid gradients by lipid counter-flows.

    abstract::Lipids are unevenly distributed within eukaryotic cells, allowing various processes to work in an optimized membrane environment. Rather than following vesicular trafficking pathways, certain lipids are transported by lipid transfer proteins (LTPs), some at sites of close apposition of two organelles called membrane c...

    journal_title:Biochimie

    pub_type: 杂志文章,评审

    doi:10.1016/j.biochi.2016.08.001

    authors: Moser von Filseck J,Drin G

    更新日期:2016-11-01 00:00:00

  • The relationship between HDL-, LDL-, liposomes-free cholesterol, biliary cholesterol and bile salts in the rat.

    abstract::In order to study the relationship between bile cholesterol and free cholesterol carried by high and low density lipoproteins (HDL and LDL), 10 male Wistar rats, 11 weeks old and fed with a standard diet were divided into 3 groups which received an intravenous infusion (jugular vein) of either LDL, HDL or liposomes. L...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/0300-9084(87)90270-7

    authors: Esnault-Dupuy C,Chanussot F,LaFont H,Chabert C,Hauton J

    更新日期:1987-01-01 00:00:00

  • Early effects of triidothyronine on the complexity of rat heart messenger RNAs.

    abstract::Triiodothyronine (T3), injected daily into rats, induces heart hypertrophy. In a recent work we have shown that a single T3 injection stimulates RNA synthesis and modifies the translational products of myocardial mRNAs in reticulocyte lysates, quantitatively and qualitatively. In this work we show that T3 induces smal...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/0300-9084(84)90113-5

    authors: Raymondjean M,Leibovitch SA,Leibovitch MP,Harel J,Kruh J

    更新日期:1984-09-01 00:00:00

  • Herba epimedii flavonoids suppress osteoclastic differentiation and bone resorption by inducing G2/M arrest and apoptosis.

    abstract::Accumulating evidences suggest that Herba epimedii has the potential benefits against osteoporosis. However, previous studies were focused on the crude extract, total flavonoids (TF) and icariin (ICA), and the detailed molecular mechanisms of action and structure-activity relationship (SAR) remain unclear. Herein we a...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2012.06.033

    authors: Zhang D,Zhang J,Fong C,Yao X,Yang M

    更新日期:2012-12-01 00:00:00

  • A method for deciding whether two experimental fluorescence anisotropy decay curves are significantly different.

    abstract::We describe a method to compare two fluorescence anisotropy decay curves. After numerical deconvolution of both decays by a non a priori method [1], their difference, D, is considered. The variance is computed for each point of D. A confidence interval is defined which allows a decision to be made as to the significan...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/0300-9084(84)90117-2

    authors: Genest D,Privat JP

    更新日期:1984-09-01 00:00:00

  • Biochemical characterization, stability studies and N-terminal sequence of a bi-functional inhibitor from Phaseolus aureus Roxb. (Mung bean).

    abstract::Herein, we report the purification and biochemical characterization of a novel bi-functional protein proteinase/amylase inhibitor from the dietary leguminous pulse Phaseolus aureus Roxb. (Vigna radiata L.) by means of acetic acid precipitation, salt fractionation, ion-exchange chromatography (DEAE-cellulose) and affin...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2005.05.007

    authors: Haq SK,Atif SM,Khan RH

    更新日期:2005-12-01 00:00:00

  • Fatty acid biosynthesis in microorganisms being used for Single Cell Oil production.

    abstract::Single cell oils (SCOs) are now produced by various microorganisms as commercial sources of arachidonic acid (ARA) and docosahexaenoic acid (DHA). These oils are now used extensively as dietary supplements in infant formulas. An understanding of the underlying biochemistry and genetics of oil accumulation in such micr...

    journal_title:Biochimie

    pub_type: 杂志文章,评审

    doi:10.1016/j.biochi.2004.09.017

    authors: Ratledge C

    更新日期:2004-11-01 00:00:00

  • Assembly of chloroplast cytochromes b and c.

    abstract::The synthesis of holocytochromes in plastids is a catalyzed process. Several proteins, including plastid CcsA, Ccs1, possibly CcdA and a thioredoxin, plus at least two additional Ccs factors, are required in sub-stoichiometric amounts for the conversion of apocytochromes f and c(6) to their respective holoforms. CcsA,...

    journal_title:Biochimie

    pub_type: 杂志文章,评审

    doi:10.1016/s0300-9084(00)00605-2

    authors: Nakamoto SS,Hamel P,Merchant S

    更新日期:2000-06-01 00:00:00

  • Protective cellular immunity against P. falciparum malaria merozoites is associated with a different P7 and P8 residue orientation in the MHC-peptide-TCR complex.

    abstract::Developing a logical and rational methodology for obtaining vaccines, especially against the main parasite causing human malaria (P. falciparum), consists of blocking receptor-ligand interactions. Conserved peptides derived from proteins involved in invasion and having high red blood cell binding ability have thus bee...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2005.07.006

    authors: Patarroyo ME,Salazar LM,Cifuentes G,Lozano JM,Delgado G,Rivera Z,Rosas J,Vargas LE

    更新日期:2006-02-01 00:00:00

  • [Energy-dependent translocation of amino-phospholipids in the erythrocyte membrane].

    abstract::In this review, we show how the stability of the asymmetric transverse distribution of phospholipids and the physiological role of the asymmetric distribution can be explained. Experiments with paramagnetic or fluorescent lipids enabled us to show that in fresh red blood cells, i.e. containing ATP, and in resealed gho...

    journal_title:Biochimie

    pub_type: 杂志文章,评审

    doi:10.1016/s0300-9084(86)80005-0

    authors: Devaux PF,Zachowski A,Favre E,Fellmann P,Cribier S,Geldwerth D,Hervé P,Seigneuret M

    更新日期:1986-03-01 00:00:00

  • Thyroglobulin structure and function: recent advances.

    abstract::Thyroglobulin is a large-size iodoglycoprotein specific to thyroid tissue and is the substrate for the synthesis of thyroid hormones, thyroxine and 3,5,3'-triiodothyronine. Recent studies, which greatly benefited from recombinant DNA methodologies, improved the knowledge of several structural features of this dimeric ...

    journal_title:Biochimie

    pub_type: 杂志文章,评审

    doi:10.1016/0300-9084(89)90057-6

    authors: Malthièry Y,Marriq C,Bergé-Lefranc JL,Franc JL,Henry M,Lejeune PJ,Ruf J,Lissitzky S

    更新日期:1989-02-01 00:00:00

  • Interaction of phosphonates related to glutathione with the rat kidney gamma-glutamylcysteine synthetase.

    abstract::Various phosphonic and sulfonic glutamate analogues as well as phosphonopeptides related to glutathione were studied for their interaction with rat kidney gamma-glutamylcysteine synthetase activity. We found, in all cases, that the presence of a phosphonic group increases the affinity for the enzyme. Among the tripept...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/0300-9084(87)90083-6

    authors: Marche M,Basurko MJ,Cassaigne A

    更新日期:1987-05-01 00:00:00

  • Variations in some molecular events during the early phases of the Reuber H 35 cell cycle. II.-Chromatin protein phosphorylation and protein kinases.

    abstract::Reuber H 35 hepatoma cells were synchronized by transfer in a serum free medium. Growth was re-initiated by addition of serum. Under these conditions DNA synthesis exhibited a maximum after 24 hours. Chromatin non-histone proteins prepared from cells at various phases of the cell cycle were incubated with [gamma-32P] ...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/s0300-9084(79)80280-1

    authors: Defer N,Tichonicky L,Kruh J,Zoutewelle G,Van Wijk R

    更新日期:1979-01-01 00:00:00