Abstract:
:Oligonucleotides that complement Escherichia coli 16S ribosomal RNA residues 685-696 and 694-705 have been synthesized so as to incorporate antibody-recognizable markers: a 3'-terminal residue of N6-delta 2-isopentenyladenosine, a 5'-dinitrophenyl group, or both. Each oligonucleotide is able to bind RNA within the small ribosomal subunit, whether free or in 70S ribosomes. Immune electron microscopy places probes at nucleotides 685, 694 and 705 within a single area, at the tip of the subunit platform, very near the position of the 3'-end of the 16S RNA.
journal_name
Biochimiejournal_title
Biochimieauthors
McWilliams RA,Glitz DGdoi
10.1016/0300-9084(91)90132-ksubject
Has Abstractpub_date
1991-07-01 00:00:00pages
911-8issue
7-8eissn
0300-9084issn
1638-6183pii
0300-9084(91)90132-Kjournal_volume
73pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract::Adenosine to inosine (A-to-I) modification by the ADAR (adenosine deaminase that acts on RNA) enzymes perform the most common type of RNA editing in metazoans. ADARs use double stranded RNA as substrates but allow interruptions of bulges and loops in the structure. It is well known that these enzymes can use messenger...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2007.06.002
更新日期:2007-10-01 00:00:00
abstract::Despite its biological importance, iron is a pro-oxidant element and its accumulation results in tissue injury. Iron overload diseases such as thalassemia and hereditary hemochromatosis are commonly associated with liver tissue injury. Glutamyl cysteine (GC) is a dipeptide with antioxidant properties owing to its cyst...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2015.06.006
更新日期:2015-08-01 00:00:00
abstract::Dyskeratosis congenita is an inherited syndrome characterised by mucocutaneous features, bone marrow failure, an increased risk of malignancy and other somatic abnormalities. There is a considerable range of clinical severity and in its occult form the disease may present as idiopathic aplastic anaemia. Genes responsi...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2007.07.017
更新日期:2008-01-01 00:00:00
abstract::We report Raman spectra of various cholinesterases: lytic tetrameric forms (G4) obtained by tryptic digestion of asymmetric acetylcholinesterase (AChE) from Torpedo californica and Electrophorus electricus, a PI-PLC-treated dimeric form (G2) of AChE from T marmorata, and the soluble tetrameric form (G4) of butyrylchol...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(91)90167-y
更新日期:1991-11-01 00:00:00
abstract::The human ribosomal protein SA, known also as a precursor of the cell-surface laminin receptor, LAMR, is a protein of the 40S ribosomal subunit. It is homologous to eubacterial ribosomal protein S2p, but has a eukaryote-specific C-terminal domain (CTD) that is responsible in LAMR for the binding of laminin as well as ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2010.12.005
更新日期:2011-03-01 00:00:00
abstract::The imprinted gene Grb10 influences fetal growth, adult behavior and tumor formation. Investigating this gene often involves consecutive passaging of cells in vitro. We analyzed changes in Grb10 DNA methylation and histone modifications in serially passaged adult mouse tail-tip fibroblast cells with bisulfite genomic ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2012.08.009
更新日期:2012-12-01 00:00:00
abstract::Ten new defensins have been isolated from seeds of Triticum kiharae and related species of the Triticum and Aegilops genera by a combination of chromatographic procedures including affinity-, size-exclusion, and reversed-phase high-performance liquid chromatography. Nine were completely sequenced and shown to represen...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2006.09.009
更新日期:2007-05-01 00:00:00
abstract::We have proposed an allosteric ATP inhibition mechanism of Pfk-2 determining the structure of different forms of the enzyme together with a kinetic enzyme analysis. Here we complement the mechanism by using hybrid oligomers of the homodimeric enzyme to get insights about the allosteric communication pathways between t...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2016.08.013
更新日期:2016-09-01 00:00:00
abstract::The open reading frame PA3859 of Pseudomonas aeruginosa encodes an intracellular carboxylesterase belonging to a group of microbial enzymes (EC 3.1.1.1) that catalyze the hydrolysis of aliphatic and aromatic esters with a broad substrate specificity. With few exceptions, for this class of enzymes, belonging to the α/β...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2010.09.001
更新日期:2010-12-01 00:00:00
abstract::The short isoform of Rho guanine nucleotide exchange factor ARHGEF5 is known as TIM, which plays diverse roles in, for example, tumorigenesis, neuronal development and Src-induced podosome formation through the activation of its substrates, the Rho family of GTPases. The activation is auto-inhibited by a putative heli...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2015.01.011
更新日期:2015-04-01 00:00:00
abstract::Two beta-D-glycosidases have been purified from the digestive juice of Achatina balteata by acetone fractionation, ion exchange chromatography through DEAE-Sephadex A-50, ammonium sulphate fractionation and gel chromatography through Sephadex G-200. The preparations are homogeneous by p/lyacrylamide gel electrophoresi...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(77)80167-3
更新日期:1977-01-01 00:00:00
abstract::Pentatricopeptide repeat (PPR) proteins are a large family of modular RNA-binding proteins which mediate several aspects of gene expression primarily in organelles but also in the nucleus. These proteins facilitate processing, splicing, editing, stability and translation of RNAs. While major advances in PPR research h...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2015.04.004
更新日期:2015-06-01 00:00:00
abstract::A depletion in long-chain polyunsaturated omega3 fatty acids may affect fuel homeostasis. In such a perspective, the present study deals mainly with the in vitro fate of D-[U-(14)C]glucose in hemidiaphragms, stretched soleus and plantaris muscle pieces obtained from normal and omega3-depleted rats (second generation) ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2006.10.004
更新日期:2007-03-01 00:00:00
abstract::Triiodothyronine (T3), injected daily into rats, induces heart hypertrophy. In a recent work we have shown that a single T3 injection stimulates RNA synthesis and modifies the translational products of myocardial mRNAs in reticulocyte lysates, quantitatively and qualitatively. In this work we show that T3 induces smal...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(84)90113-5
更新日期:1984-09-01 00:00:00
abstract::Human Rad51 (HsRad51), a key element of the homologous recombination repair pathway, is related to the resistance of cancer cells to chemo- and radio-therapies. This protein is thus a good target for the development of anti-cancer treatments. We have searched for new inhibitors directed against HsRad51 using the Syste...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2010.08.006
更新日期:2010-12-01 00:00:00
abstract::The distribution of fatty acids among cellular glycerophospholipids is finely regulated by the CoA-dependent acylation of lysophospholipids followed by transacylation reactions. Arachidonic acid is the fatty acid precursor of a wide family of bioactive compounds called the eicosanoids, with key roles in innate immunit...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2009.11.010
更新日期:2010-06-01 00:00:00
abstract::The proton NMR data of a synthetic model of deoxy-myoglobin and hemoglobin show one that:(i) the ground state of the high spin iron (II) complex must be described by at least two electronic levels separated by ca.200 cm-1 and resulting from the removal of the degeneracy of the 5E level; (ii) this in-plane rhombic dist...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(82)80285-x
更新日期:1981-11-01 00:00:00
abstract::Seryl-tRNA synthetases (SerRS) covalently attach serine to cognate tRNA(Ser). Atypical SerRSs, considerably different from canonical enzymes, have been found in methanogenic archaea. A crystal structure of methanogenic-type SerRS revealed a motif within the active site (serine ordering loop; SOL), which undergoes a no...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2011.06.013
更新日期:2011-10-01 00:00:00
abstract::Plasmalogens (Pls) represent a specific subclass of glycerophospholipids characterized by the presence of a vinyl-ether bond at the sn-1 position of glycerol. Pls are quantitatively important in membranes of neuronal tissues, including the brain and the retina, where they can represent until almost two-third of ethano...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2014.07.023
更新日期:2014-12-01 00:00:00
abstract::1) Isonitrile derivatives of synthetic polyamides, polyesters and polyalcohols, of polysaccharides such as cellulose, cross-linked dextran and agarose, linear dextran and of cross-linked and linear polyacrylamide were prepared. 2) Proteins and low-molecular-weight ligands were bound covalently to these materials by fo...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(80)80172-6
更新日期:1980-01-01 00:00:00
abstract::Since the initial identification of leptin as the product of the ob gene in 1994, the signaling pathways by which this hormone alters cell physiology have been the subject of extensive investigations. The fact that leptin can induce nitric oxide (NO) production was first demonstrated in studies of the pituitary gland ...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2012.06.017
更新日期:2012-10-01 00:00:00
abstract::Berardinelli-Seip congenital lipodystrophy (BSCL) is a rare recessive disease characterized by near absence of adipose tissue and severe insulin resistance. In most cases, BSCL is due to loss-of-function mutations in the genes encoding either seipin of unknown function or 1-acyl-glycerol-3-phosphate O-acyltransferase ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2009.01.011
更新日期:2009-06-01 00:00:00
abstract::Antibodies to Guo-AF were elicited in rabbits by immunization with bovine serum albumin -Guo-AF conjugate. The antibodies were purified by affinity chromatography. The affinity of the antibodies towards several ligands was studied by radioimmunoassays. The antibodies recognize Gua-AAF and Guo-AF (K(Guo-AF)/K(Guo-AAF) ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(80)80066-6
更新日期:1980-01-01 00:00:00
abstract::A higher level of N-acetylglucosamine incorporation by proteinic and polyprenic endogenous acceptors is observed after infection by Myxovirus. This phenomenon occurs in whole mitochondria as in outer mitochondrial membranes, where it is particularly obvious with proteinic endogenous acceptors. Under viral infection, n...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(80)80373-7
更新日期:1980-01-01 00:00:00
abstract::GABARAPL1 belongs to the small family of GABARAP proteins (including GABARAP, GABARAPL1 and GABARAPL2/GATE-16), one of the two subfamilies of the yeast Atg8 orthologue. GABARAPL1 is involved in the intracellular transport of receptors, via an interaction with tubulin and GABA(A) or kappa opioid receptors, and also par...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2011.11.006
更新日期:2012-03-01 00:00:00
abstract::Acinetobacter baumannii encodes all enzymes required in the tricarboxylic acid (TCA) cycle and glyoxylate bypass except for isocitrate dehydrogenase kinase/phosphatase (IDHKP), which can phosphorylate isocitrate dehydrogenase (IDH) at a substrate-binding Ser site and control the carbon flux in enterobacteria, such as ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2020.12.001
更新日期:2020-12-05 00:00:00
abstract::In relation to decreased metabolic sensitivity to catecholamines observed, in vitro, in brown fat of cold-acclimated rats, beta-adrenergic receptors were studied in isolated cells and in a crude membrane preparation from rat interscapular brown adipose tissue. [3H] dihydroalprenolol binding had the same characteristic...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(84)90153-6
更新日期:1984-07-01 00:00:00
abstract::The effect of 7-chloro-4-nitrobenzofurazan on yeast 3-phosphoglycerate kinase causes a modification of one tyrosyl residue concomitantly with a total loss of activity of the enzyme. The modification is not accompanied by any significant conformational change. A total protection against inactivation is observed with th...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(79)80164-9
更新日期:1979-01-01 00:00:00
abstract::Dihydroorotate dehydrogenase (DHODH) is the fourth enzyme in the de novo pyrimidine biosynthetic pathway and has been exploited as the target for therapy against proliferative and parasitic diseases. In this study, we report the crystal structures of DHODH from Leishmania major, the species of Leishmania associated wi...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2012.04.003
更新日期:2012-08-01 00:00:00
abstract::We have previously identified a protein with Mr approximately 40,000 (p40) that binds with high specificity and affinity to the 5'-untranslated leaders of mitochondrial mRNAs in yeast. Here we show that this protein is abundant, comprising about 0.4% of total mitochondrial protein. p40 is present in a cytoplasmic (rho...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(91)90182-z
更新日期:1991-12-01 00:00:00