Abstract:
:In this study, peptidomics and genomics analyses were used to study antimicrobial peptides from the skin of Hylarana spinulosa. Twenty-nine different antimicrobial peptide precursors were characterized from the skin of H. spinulosa, which produce 23 mature antimicrobial peptides belonging to 12 different families. To confirm the actual presence and characteristics of these antimicrobial peptides in the skin tissue extractions from H. spinulosa, we used two distinct methods, one was peptide purification method that combined gel filtration chromatography and reversed-phase high performance liquid chromatography (RP-HPLC), and the other was peptidomics approach based on liquid chromatography in conjunction with tandem mass spectrometry (LC-MS/MS). In the peptidomics approach, incomplete tryptic digestion and gas-phase fractionation (GPF) analysis were used to increase peptidome coverage and reproducibility of peptide ion selection. Multiple species of microorganisms were chosen to test and analyze the antimicrobial activities and spectrum of these antimicrobial peptides.
journal_name
Biochimiejournal_title
Biochimieauthors
Yang X,Hu Y,Xu S,Hu Y,Meng H,Guo C,Liu Y,Liu J,Yu Z,Wang Hdoi
10.1016/j.biochi.2013.09.002subject
Has Abstractpub_date
2013-12-01 00:00:00pages
2429-36issue
12eissn
0300-9084issn
1638-6183pii
S0300-9084(13)00316-7journal_volume
95pub_type
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