Calcium, magnesium and the conformation of parvalbumin during muscular activity.

abstract：:P53 is a transcription factor also called the "cellular gatekeeper of genome" because it can induce cell cycle arrest in G1, apoptosis or affect DNA replication in response to DNA damage. Wild type p53 is localised in both the cytoplasm and nucleus of first trimester trophoblastic cells (CTB). Immunoblotting of CTB wi...

journal_title：Biochimie

authors： Cohen M,Wuillemin C,Bischof P

更新日期：2008-06-01 00:00:00

abstract：:A Binding Protein (BP) of nearly 95,000 daltons interacts with the acid phosphatase at the permissive temperature, elevating its molecular weight from 48,000 +/- 2,000 to 136,000 +/- 7,000 in CL and CHR strains of Physarum polycephalum plasmodia. The same molecule has not been observed in the extracts of M3C VIII and ...

journal_title：Biochimie

authors： Demirtas H

更新日期：1983-06-01 00:00:00

abstract：:A soluble hydrogenase from the halophilic sulfate reducing bacterium Desulfovibrio salexigens, strain British Guiana (NCIB 8403) has been purified to apparent homogeneity with a final specific activity of 760 mumoles H2 evolved/min/mg (an overall 180-fold purification with 20% recovery yield). The enzyme is composed o...

journal_title：Biochimie

authors： Teixeira M,Moura I,Fauque G,Czechowski M,Berlier Y,Lespinat PA,Le Gall J,Xavier AV,Moura JJ

更新日期：1986-01-01 00:00:00

abstract：:Human α(1)-acid glycoprotein (AGP) is a positive acute phase plasma protein containing two disulfide bridges. Structural studies have shown that under specific conditions AGP undergoes aggregation. In this study, we analysed the nature of AGP's aggregates formed under reducing and non-reducing conditions at pH 5.5 and...

journal_title：Biochimie

authors： Scirè A,Baldassarre M,Galeazzi R,Tanfani F

更新日期：2013-02-01 00:00:00

abstract：:Unlike most tonoplast ATPases, the vacuolar ATPase of Acer pseudoplatanus cells (Km = 0.4 mM) was strongly inhibited by vanadate (I50 = 10 microM). The inhibition was non-competitive. Chemicals usually added in the reaction mixture either increase (NH+4, K+) or decrease (Na+, EDTA) the ATPase inhibition. However, thes...

journal_title：Biochimie

authors： Montrichard F,Pugin A,Gaudemer Y

更新日期：1989-07-01 00:00:00

abstract：:Bacterial survive and respond to adverse changes in the environment by regulating gene transcription through two-component regulatory systems. In Salmonella Typhimurium the STM1485 gene expression is induced under low pH (4.5) during replication inside the epithelial host cell, but it is not involved in sensing or res...

journal_title：Biochimie

authors： Torrez Lamberti MF,Farizano JV,López FE,Martínez Zamora MG,Pescaretti MM,Delgado MA

更新日期：2019-05-01 00:00:00

abstract：:The complete amino acid sequences of 3 proteins from the 50S subunit of Bacillus stearothermophilus ribosomes were determined by N-terminal sequence analysis and by sequencing of overlapping fragments obtained from enzymatic digestions and chemical cleavages. The proteins BstL28, BstL33 and BstL34, named according to ...

journal_title：Biochimie

authors： Kruft V,Kapp U,Wittmann-Liebold B

更新日期：1991-07-01 00:00:00

abstract：:Translational control is a common mechanism used to regulate gene expression and occur in bacteria to mammals. Typically in translational control, an RNA-binding protein binds to a unique sequence in the mRNA to regulate protein synthesis by the ribosomes. Alternatively, a protein may bind to or modify a translation f...

journal_title：Biochimie

authors： Chen E,Joseph S

更新日期：2015-07-01 00:00:00

abstract：:Zika and Dengue viruses have attracted substantial attention from researchers in light of recent outbreaks of Dengue fever and increases in cases of congenital microcephaly in areas with Zika incidence. This review summarizes the current state of knowledge about Zika and Dengue proteases. These enzymes have several in...

journal_title：Biochimie

authors： Majerová T,Novotný P,Krýsová E,Konvalinka J

更新日期：2019-11-01 00:00:00

abstract：:Genetic elements coding for proteins that present amino acid identity with the conserved motifs of retroviral reverse transcriptases constitute the retroid family. With the exception of reverse transcriptases encoded by mitochondrial plasmids of Neurospora, all reverse transcriptases have an absolute requirement for a...

journal_title：Biochimie

authors： Marquet R,Isel C,Ehresmann C,Ehresmann B

更新日期：1995-01-01 00:00:00

abstract：:Recombinant plasmids containing head-to-tail copies of different coat-protein replacement genomes of wheat dwarf virus (WDV) were used to study the mechanism leading to the release of replicating unit-length molecules in suspension culture cells of Triticum monococcum. For plasmids bearing two complete genomes, the vi...

journal_title：Biochimie

authors： Heyraud F,Matzeit V,Schaefer S,Schell J,Gronenborn B

更新日期：1993-01-01 00:00:00

abstract：:The termination of protein synthesis in bacteria requires codon-specific polypeptide release factors RF-1 (UAG/UAA specific) and RF-2 (UGA/UAA specific). We have proposed that release factors mimic tRNA and recognize the stop codon for polypeptide release (Nakamura et al (1996) Cell 87, 147-150). In contrast to the te...

journal_title：Biochimie

authors： Uno M,Ito K,Nakamura Y

更新日期：1996-01-01 00:00:00

abstract：:We isolated and purified the main lipids of the rat sciatic nerve. After methanolysis, fatty acids were isolated and purified by thin layer chromatography, and studied by gas chromatography. C 16 and C 18 fatty acids are the most abundant. Among the long-chain fatty acids, arachidonic acid (20:4) is present in some li...

journal_title：Biochimie

authors： Klein F,Mandel P

更新日期：1978-01-01 00:00:00

abstract：:Current research in biology heavily depends on the availability and efficient use of information. In order to build new knowledge, various sources of biological data must often be combined. Semantic Web technologies, which provide a common framework allowing data to be shared and reused between applications, can be ap...

journal_title：Biochimie

authors： Pasquier C

更新日期：2008-04-01 00:00:00

abstract：:Phosphoenolpyruvate carboxykinase (PEPCK) is the key enzyme in glyceroneogenesis, an important metabolic pathway that functions to restrain the release of non-esterified fatty acids (NEFAs) from adipocytes. The antidiabetic drugs known as thiazolidinediones (TZDs) are thought to achieve some of their benefits by lower...

journal_title：Biochimie

authors： Glorian M,Duplus E,Beale EG,Scott DK,Granner DK,Forest C

更新日期：2001-10-01 00:00:00

abstract：:Heat shock proteins (Hsps) are involved in several important aspects of the cell proteostasis. Hsp90 interacts with at least a tenth of the cell proteome helping a large number of proteins to fold correctly. Hsp90 function is modulated by several co-chaperones having TPR (tetratricopeptide repeat) domains that allow f...

journal_title：Biochimie

authors： Araujo SA,Martins GH,Quel NG,Aragão AZB,Morea EGO,Borges JC,Houry WA,Cano MIN,Ramos CHI

更新日期：2021-01-06 00:00:00

abstract：:Glutaredoxins (Grxs), redox-active proteins with a typical -CPYC motif at their active sites, are involved in redox-regulatory processes and antioxidant defenses. The human malarial parasite Plasmodium falciparum possess a classical glutaredoxin (PfGrx) as well as a number of Grx-like proteins. In the present study, w...

journal_title：Biochimie

authors： Tripathi T,Röseler A,Rahlfs S,Becker K,Bhakuni V

更新日期：2010-03-01 00:00:00

abstract：:Neuropeptides and hormones, in contrast to constitutive secretory proteins, are sorted to and stored in secretory granules and released upon a stimulus. During the last two decades, signals and mechanisms involved in their sorting to the regulated pathway of protein secretion have been addressed in numerous studies. T...

journal_title：Biochimie

authors： Glombik MM,Gerdes HH

更新日期：2000-04-01 00:00:00

abstract：:Conformational flexibility and structural fluctuations play an important role in enzyme activity. A great variety of internal motions ranging over different time scales and of different amplitudes are involved in the catalytic cycle. These different types of motions and their functional consequences are considered in ...

journal_title：Biochimie

authors： Yon JM,Perahia D,Ghélis C

更新日期：1998-01-01 00:00:00

abstract：:We have produced and purified an active site mutant of the Escherichia coli cyclopropane fatty acid synthase (CFAS) by replacing the strictly conserved G236 within cyclopropane synthases, by a glutamate residue, which corresponds to E146 of the homologous mycolic acid methyltransferase, Hma, producing hydroxymethyl my...

journal_title：Biochimie

authors： E G,Drujon T,Correia I,Ploux O,Guianvarc'h D

更新日期：2013-12-01 00:00:00

abstract：:Surfactants carrying either a hydrocarbon or a fluorocarbon alkyl chain have been synthesized. The polar head was either tris(hydroxymethyl)acrylamidomethane (THAM), telomerized THAM, or a glycosylated THAM moiety. The aqueous solubility of some of these molecules was increased by oxidizing to a sulfoxide the thioethe...

journal_title：Biochimie

authors： Chabaud E,Barthélémy P,Mora N,Popot JL,Pucci B

更新日期：1998-05-01 00:00:00

abstract：:Binding of indoloquinolines with different aliphatic side chains to a parallel G-quadruplex derived from the MYC promoter sequence was characterized by optical and calorimetric measurements. ITC experiments performed at different temperatures enabled the determination of molar heat capacity changes upon quadruplex bin...

journal_title：Biochimie

authors： Funke A,Weisz K

更新日期：2019-02-01 00:00:00

abstract：:Endothelial nitric oxide synthase (eNOS) plays a crucial role in the regulation of a variety of cardiovascular functions. Many studies have shown that dietary n-3 polyunsaturated fatty acids (PUFAs) have beneficial effects on coronary atherosclerosis. However, the mechanisms of n-3 PUFAs regulation in eNOS activation ...

journal_title：Biochimie

authors： Li Q,Zhang Q,Wang M,Zhao S,Ma J,Luo N,Li N,Li Y,Xu G,Li J

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND:The modest efficacy of available therapies for Hepatocellular carcinoma (HCC) indicates the need to develop novel therapeutic approaches. For the proteasome inhibitor Bortezomib (BZB), potentially attractive for HCC treatment, the mechanism of action is largely unknown. The BZB effect on E2Fs and the E2Fs co...

journal_title：Biochimie

authors： Farra R,Dapas B,Baiz D,Tonon F,Chiaretti S,Del Sal G,Rustighi A,Elvassore N,Pozzato G,Grassi M,Grassi G

更新日期：2015-05-01 00:00:00

abstract：:Stroke is a worldwide major cause of mortality and morbidity. Preclinical studies have identified over 1000 molecules with brain-protective properties. More than 200 clinical trials have evaluated neuroprotective candidates for ischemic stroke yet, to date almost all failed, leading to a re-analysis of treatment strat...

journal_title：Biochimie

authors： Blondeau N

更新日期：2016-01-01 00:00:00

abstract：:We have purified, by a three step procedure, a yeast C-nitrosoreductase and studied its action on p-nitroso-N,N-dimethylaniline. Microcalorimetric measurements performed on the microcalorimeter LKB "batch", have been given the number of protons exchanged during the reaction : four protons are involved. Two are given b...

journal_title：Biochimie

authors： Huynh CH

更新日期：1975-01-01 00:00:00

abstract：:Murine L5178Y cell ribosomes were dissociated into subunits either with potassium chloride in the presence of puromycin or with the chelating agent EDTA. The proteins of ribosomal subunits obtained by these different methods were compared by means of bidimensional polyacrylamide gel electrophoresis. KCl-derived 60S an...

journal_title：Biochimie

authors： Longuet M,Auger-Buendia MA,Tavitian A

更新日期：1979-01-01 00:00:00

abstract：:Previous work from this laboratory has shown that T7 RNA polymerase outpaces ribosomes in vivo, generating naked mRNA stretches which may be nuclease-sensitive. In particular, lacZ transcripts synthesised this way are highly unstable and yield little beta-galactosidase. We have argued that most of these transcripts ar...

journal_title：Biochimie

authors： Lopez PJ,Dreyfus M

更新日期：1996-01-01 00:00:00

abstract：:Lipidomics is a subspecialty of metabolomics that focuses on water insoluble metabolites that form membrane barriers. Most lipidomic databases catalog lipids from common model organisms, like humans or Escherichia coli. However, model organisms' lipid profiles show surprisingly little overlap with those of specialized...

journal_title：Biochimie

authors： Layre E,Moody DB

更新日期：2013-01-01 00:00:00

abstract：:The genus Populus is an effective model in tree genetics. This genus includes dioecious species and, recently, whole genome resequencing of P. trichocarpa and P. balsamifera enabled the identification of sex-linked regions and sex-associated single nucleotide polymorphisms (SNPs). These results created new opportuniti...

journal_title：Biochimie

authors： Melnikova NV,Kudryavtseva AV,Borkhert EV,Pushkova EN,Fedorova MS,Snezhkina AV,Krasnov GS,Dmitriev AA

更新日期：2019-07-01 00:00:00