Abstract：
:The central glycolytic and oxidative pathways and the ATP-producing mechanisms differ in sane and malignant cells by their regulation and dynamics. Fast-growing, poorly-differentiated cancer cells characteristically show high aerobic glycolysis. In the same way, cholesterol biosynthesis, which occurs by normal pathways in tumors, is deficient in feed-back regulation and in sterol-transport mechanisms. Other metabolic ways are deficient, as for example, intramitochondrial aldehyde catabolism, at the origin of a possible acetaldehyde toxicity, which can be circumvented by the synthesis of an unusual and neutral product for mammalian cells acetoin, through tumoral pyruvate dehydrogenase. If most of the glycolytic pyruvate is deviated to lactate production, little of the remaining carbons enter a truncated Krebs cycle where citrate is preferentially extruded to the cytosol where it feeds sterol synthesis. Glutamine is the major oxidizable substrate by tumor cells. Inside the mitochondrion, it is deaminated to glutamate through a phosphate-dependent glutaminase. Glutamate is then preferentially transaminated to alpha-ketoglutarate that enters the Krebs cycle. Glutamine may be completely oxidized through the abnormal Krebs cycle only if a way of forming acetyl CoA is present: cytosolic malate entering mitochondria is preferentially oxidized to pyruvate + CO2 through an intramitochondrial NAD(P)(+)-malic enzyme, whereas intramitochondrial malate is preferentially oxidized to oxaloacetate through malate dehydrogenase, thus providing a high level of intramitochondrial substrate compartmentation. These and other regulatory aberrations in tumor cells appear to be reflections of a complex set of non-random phenotypic changes, initiated by expression of oncogenes.
journal_name
Biochimiejournal_title
Biochimieauthors
Baggetto LGdoi
10.1016/0300-9084(92)90016-8keywords:
subject
Has Abstractpub_date
1992-11-01 00:00:00pages
959-74issue
11eissn
0300-9084issn
1638-6183pii
0300-9084(92)90016-8journal_volume
74pub_type
杂志文章,评审相关文献
BIOCHIMIE文献大全abstract：:Tetramolecular G-quadruplexes result from the association of four guanine-rich strands. Modification of the backbone strand or the guanine bases of the oligonucleotide may improve stability or introduce new functionalities. In this regard, the 8 position of a guanosine is particularly suitable for introduction of modi...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2010.10.011
更新日期：2011-03-01 00:00:00
abstract：:Kinetic studies indicate that glucose-6-phosphatase is a multifunctional enzyme. a) Phosphohydrolase activities. The mannose-6-phosphatase activity is low (Km = 8 mM, VM = 90 nmoles. min-1mg-1). The enzyme shows a strong affinity for glucose-6-phosphate (Km = 2.5 mM, VM = 220 nmoles.min-1mg-1). beta-glycerophosphate (...
journal_title：Biochimie
pub_type： 杂志文章
doi：
更新日期：1979-01-01 00:00:00
abstract：:A nuclear poly(ADP-ribose) polymerase (PARP) is activated by gamma-irradiation and consequently synthesizes poly(ADP-ribose) by binding to DNA strand-breaks. This property suggests that PARP is a DNA strand-break-signal generator. Meanwhile, the cell-cycle arrest occurs in G1 and G2 phases following gamma-irradiation....
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/0300-9084(96)88161-2
更新日期：1995-01-01 00:00:00
abstract：:A HU-like protein (HBl) of Bifidobacterium longum was purified and characterized. HBl is heat-stable and acid-resistant, and has a molecular weight of about 9.1 kDa as estimated by its mobility on electrophoresis. HBl is intermediate in basicity (pI 9.8) between the HU-1 and HU-2 proteins of Escherichia coli, and is d...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(90)90074-q
更新日期：1990-04-01 00:00:00
abstract：:Nephropathy is one of the most frequent complications of chronic diabetes. The main reason for nephropathy despite being hyperglycemia, but it progresses even after good glycemic control has been achieved in diabetic patients. The effects of prior exposure to high blood glucose conditions depend upon the severity and ...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2020.01.007
更新日期：2020-03-01 00:00:00
abstract：:Epigenetic mechanisms are likely to play a role in many complex diseases, the extent of which we only beginning to understand. COPD and asthma are two respiratory diseases subject to strong environmental influences depending on underlying genetic susceptibility. Epigenetic mechanisms such as DNA methylation, histone m...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2012.07.017
更新日期：2012-11-01 00:00:00
abstract：:The published literature contains a number of examples of normally non-cytoplasmic proteins whose transport out of the cytoplasm is not completely abolished by drastic alterations to their routing signals (signal sequences, etc). Furthermore, there are numerous examples of cytoplasmic proteins that can be routed to an...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/0300-9084(90)90133-2
更新日期：1990-02-01 00:00:00
abstract：:Vascular endothelial growth factor (VEGF) and αvβ3 integrin are key molecules that actively participate in tumor angiogenesis and metastasis. Some integrin-blocking molecules are currently under clinical trials for cancer and metastasis treatment. However, the mechanism of action of such inhibitors is not completely u...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2012.04.020
更新日期：2012-08-01 00:00:00
abstract：:The fatty acid composition of animal products (eggs, milk and meat) is the reflect of both the tissue fatty acid biosynthesis and the fatty acid composition of ingested lipids. This relationship is stronger in monogastrics (pigs, poultry and rabbits) than in ruminants, where dietary fatty acids are hydrogenated in the...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2010.02.027
更新日期：2011-01-01 00:00:00
abstract：:Structural and motional features in the denatured state of a protein dictate the early folding events starting from that state and these features vary depending upon the nature of the denaturant used. Here, we have attempted to decipher the early events in the folding of Dynein Light Chain protein (DLC8), starting fro...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2011.10.013
更新日期：2012-01-01 00:00:00
abstract：:Microalgae can produce large quantities of triacylglycerols (TAGs) and other neutral lipids that are suitable for making biofuels and as feedstocks for green chemistry. However, TAGs accumulate under stress conditions that also stop growth, leading to a trade-off between biomass production and TAG yield. Recently, in ...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2019.06.016
更新日期：2020-02-01 00:00:00
abstract：:The efficiency of oxidative phosphorylation was estimated in intact resting cells of Escherichia coli K 12, strain PA 601 (chl-s) and its chl-r mutants, all of them grown anaerobically in the presence of nitrate. The oxidation of endogenous NADH in intact chl-s cells was accompanied by the formation of ATP whatever th...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(77)80316-7
更新日期：1977-01-01 00:00:00
abstract：:Two beta-D-glycosidases have been purified from the digestive juice of Achatina balteata by acetone fractionation, ion exchange chromatography through DEAE-Sephadex A-50, ammonium sulphate fractionation and gel chromatography through Sephadex G-200. The preparations are homogeneous by p/lyacrylamide gel electrophoresi...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(77)80167-3
更新日期：1977-01-01 00:00:00
abstract：:Pseudouridine [Ψ] is a frequent base modification in the ribosomal RNA [rRNA] and may be involved in the modulation of the conformational flexibility of rRNA helix-loop structures during protein synthesis. Helix 69 of 23S rRNA contains pseudouridines at the positions 1911, 1915 and 1917 which are formed by the helix 6...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2010.12.018
更新日期：2011-05-01 00:00:00
abstract：:Translational autocontrol of the expression of the ribosomal protein S15 proceeds through the transitory formation of a pseudoknot. A synopsis of the known data is used to propose a molecular model of the mechanism involved and for the role of the pseudoknot. This latter structure is able to recruit 30S ribosomal subu...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(96)80003-4
更新日期：1996-01-01 00:00:00
abstract：:LipA from Pseudomonas protegens Pf-5 has been proven not to be secreted into the extracytoplasmic space, proposing that it is a membrane protein in virtue of its N-terminal transmembrane domain predicted by the TMHMM 2.0. However, LipA was confirmed to be an intracellular protein through determining the effects of lip...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2014.07.007
更新日期：2014-10-01 00:00:00
abstract：:The ascomycete Cladosporium herbarum is a prominent fungal inducer of Type I allergy. The only major allergen identified so far is Cla h 8, a NADP-dependent mannitol dehydrogenase (MtDH). MtDH, a cytoplasmic protein of 28.5kDa, belongs to the Short chain Dehydrogenases/Reductases (SDR), acting as a NADP-dependent oxid...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2010.04.012
更新日期：2010-08-01 00:00:00
abstract：:The use of electrospun scaffolds for neural tissue engineering applications allows a closer mimicry of the native tissue extracellular matrix (ECM), important for the transplantation of cells in vivo. Moreover, the role of the electrospun fiber mat topography on neural stem cell (NSC) differentiation remains to be com...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2021.01.001
更新日期：2021-01-07 00:00:00
abstract：:The toxicity of amyloids is a subject under intense scrutiny. Many studies link this toxicity to the existence of various intermediate structures prior to the fiber formation and/or their specific interaction with membranes. Membranes can also be a catalyst of amyloidogenesis and the composition or the charge of membr...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2012.07.011
更新日期：2013-01-01 00:00:00
abstract：:rBmTI-A is a recombinant serine protease inhibitor that belongs to the Kunitz-BPTI family and that was cloned from Rhipicephalus microplus tick. rBmTI-A has inhibitory activities on bovine trypsin, human plasma kallikrein, human neutrophil elastase and plasmin with dissociation constants in nM range. It is characteriz...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2020.12.014
更新日期：2020-12-24 00:00:00
abstract：:We have previously identified a protein with Mr approximately 40,000 (p40) that binds with high specificity and affinity to the 5'-untranslated leaders of mitochondrial mRNAs in yeast. Here we show that this protein is abundant, comprising about 0.4% of total mitochondrial protein. p40 is present in a cytoplasmic (rho...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(91)90182-z
更新日期：1991-12-01 00:00:00
abstract：:In vertebrates, mannose 6-phosphate receptors [MPR300 (Mr 300 kDa) and MPR46 (Mr 46 kDa)] are highly conserved transmembrane glycoproteins that mediate transport of lysosomal enzymes to lysosomes. Our studies have revealed the appearance of these putative receptors in invertebrates such as the molluscs and deuterostom...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2012.02.007
更新日期：2012-05-01 00:00:00
abstract：:Long-term treatment with ambroxol (ABX), a bronchial expectorant, was found to prevent acute exacerbation of chronic obstructive pulmonary disease (AECOPD). The underlying mechanism remains unclear. To address this, we have investigated the effect of ABX on critical antioxidant proteins thioredoxin (Trx) and thioredox...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2013.09.024
更新日期：2014-02-01 00:00:00
abstract：:With the relation between chemical structure and pharmacological activity as a guide, we have been for some time synthetizing a wide range of beta-amino-ketone derivatives. One of them, 2-(4-methyl-1-piperazinylmethyl) acrylophenone, MPMAP, possesses antimicrotubular activities. This product inhibits 50% of the microt...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(84)90083-x
更新日期：1984-06-01 00:00:00
abstract：:Innate immune system is the first line of host defense against invading microorganisms. It relies on a limited number of germline-encoded pattern recognition receptors that recognize conserved molecular structures of microbes, referred to as pathogen-/microbe-associated molecular patterns (PAMPs/MAMPs). Bacterial cell...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2012.06.007
更新日期：2013-01-01 00:00:00
abstract：:Although up to 25% of glucose released into circulation in the postabsorptive state comes from renal gluconeogenesis, the regulatory mechanisms of this process are still poorly recognized, comparing to hepatic ones. The aim of the present study was to examine if hypoxia-inducible factor-1 (HIF-1) might be involved in ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2020.02.002
更新日期：2020-01-01 00:00:00
abstract：:Peroxisomal ABC transporters encoded by the ABCD genes are thought to participate in the import of specific fatty acids in the peroxisomal matrix. ABCD1 deficiency is associated with X-linked adrenoleukodystrophy (X-ALD), the most frequent peroxisomal disorder which is characterized by the accumulation of saturated ve...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2008.05.022
更新日期：2008-10-01 00:00:00
abstract：:Calcineurin (CN) is a unique calcium/calmodulin (CaM)-activated serine/threonine phosphatase. To perform its diverse biological functions, CN communicates with many substrates and other proteins. In the physiological activation of T cells, CN acts through transcriptional factors belonging to the NFAT family and other ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2016.04.011
更新日期：2016-08-01 00:00:00
abstract：:Classic studies suggested that the common mammalian sialic acid N-glycolylneuraminic acid (Neu5Gc) is an oncofetal antigen in humans, being immunogenic in adult humans and yet apparently expressed in human fetuses and tumors. We and others have recently found that the human deficiency of Neu5Gc can be explained by an ...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(01)01309-8
更新日期：2001-07-01 00:00:00
abstract：:Rhizopus oryzae lipase (ROL) was found to be a true lipase. This enzyme presents the interfacial activation phenomenon. The N-terminal amino acid sequence of ROL was compared to those of rhizopus lipases. Purified ROL possesses the same N-terminal sequence as the mature Rhizopus niveus lipase (RNL). This sequence was ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(01)01283-4
更新日期：2001-06-01 00:00:00
