Induction of apoptotic cell death by DNA topoisomerase II inhibitors.

Abstract:

:We have analyzed the interference of antitumoral drugs acting through the inhibition of DNA topoisomerase II on the human HeLa cell metabolism. Different compounds characterized by a diverse mechanism of action have been used, namely m-amsacrine, an intercalative drug, etoposide, which does not intercalate DNA, and suramin, which exerts its effect through an unknown mechanism. In HeLa cells treated with increasing doses of these drugs, we have examined cell viability and DNA synthesis capacity, and we have evaluated topoisomerase II activity. Cellular morphology and DNA integrity have been studied in order to characterize the mechanism of cell death. The results we have obtained clearly indicate that topoisomerase II poisons induce cell death by apoptosis. These observations suggest a role of the inhibition of topoisomerase II activity in the apoptotic program.

journal_name

Biochimie

journal_title

Biochimie

authors

Negri C,Bernardi R,Donzelli M,Scovassi AI

doi

10.1016/0300-9084(95)90009-8

subject

Has Abstract

pub_date

1995-01-01 00:00:00

pages

893-9

issue

11

eissn

0300-9084

issn

1638-6183

pii

0300-9084(95)90009-8

journal_volume

77

pub_type

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