Sequence analysis of the glyW region in Escherichia coli.

abstract：:In vitro cycling rates of E. coli ribosomes and of elongation factors EF-Tu and EF-G have been obtained and these are compatible with translation rates in vivo. We show that the rate of translocation is faster than 50 s-1 and therefore that the EF-G function is not a rate limiting step in protein synthesis. The in viv...

journal_title：Biochimie

authors： Bilgin N,Kirsebom LA,Ehrenberg M,Kurland CG

更新日期：1988-05-01 00:00:00

abstract：:Hyper- (450 mOsm/l) and hypoosmotic exposure (150 mOsm/l) of Caco-2 cells, a human intestinal epithelial cell line, induced a twofold- and a fivefold increase in the production of IL-8, a constitutively expressed cytokine, respectively. This was observed both in the presence or in the absence of added proinflammatory ...

journal_title：Biochimie

authors： Hubert A,Cauliez B,Chedeville A,Husson A,Lavoinne A

更新日期：2004-08-01 00:00:00

abstract：:The Cystic Fibrosis Transmembrane Regulator (CFTR) is a membrane protein whose mutations cause cystic fibrosis, a lethal genetic disease. We performed a molecular dynamic (MD) study of the properties of the nucleotide binding domains (NBD) whose conformational changes, upon ATP binding, are the direct responsible of t...

journal_title：Biochimie

authors： Belmonte L,Moran O

更新日期：2015-04-01 00:00:00

abstract：:As the medaka is a popular fish model in genetics, developmental biology and toxicology, the development of an efficient transgenic medaka technique is important for a variety of biological experiments. Here we demonstrated that the maize transposon system, Ac/Ds, greatly improved the transgenesis of microinjected DNA...

journal_title：Biochimie

authors： Boon Ng GH,Gong Z

更新日期：2011-10-01 00:00:00

abstract：:Various phosphonic and sulfonic glutamate analogues as well as phosphonopeptides related to glutathione were studied for their interaction with rat kidney gamma-glutamylcysteine synthetase activity. We found, in all cases, that the presence of a phosphonic group increases the affinity for the enzyme. Among the tripept...

journal_title：Biochimie

authors： Marche M,Basurko MJ,Cassaigne A

更新日期：1987-05-01 00:00:00

abstract：:The inhibition rates and spectral characteristics of 2 probes specific for the active-site serine residue of proteases were examined for evidence of conformational change of the proteases upon their binding to alpha 2-macroglobulin (alpha 2M). Elastase, chymotrypsin, trypsin, and plasmin were reacted with (7-nitrobenz...

journal_title：Biochimie

authors： Martini JL,Pochon F

更新日期：1989-03-01 00:00:00

abstract：:Ceramide synthases (CerSs) are key enzymes in the biosynthesis of ceramides and display a group of at least six different isoenzymes (CerS1-6). Ceramides itself are bioactive molecules. Ceramides with different N-acyl side chains (C(14:0)-Cer - C(26:0)-Cer) possess distinct roles in cell signaling. Therefore, the sele...

journal_title：Biochimie

authors： Schiffmann S,Hartmann D,Fuchs S,Birod K,Ferreiròs N,Schreiber Y,Zivkovic A,Geisslinger G,Grösch S,Stark H

更新日期：2012-02-01 00:00:00

abstract：:We have purified, by a three step procedure, a yeast C-nitrosoreductase and studied its action on p-nitroso-N,N-dimethylaniline. Microcalorimetric measurements performed on the microcalorimeter LKB "batch", have been given the number of protons exchanged during the reaction : four protons are involved. Two are given b...

journal_title：Biochimie

authors： Huynh CH

更新日期：1975-01-01 00:00:00

abstract：:The thyroid hormones L-thyroxine and triiodo-L-thyronine have profound effects on postembryonic development of most vertebrates. Analysis of their action in mammals is vitiated by the exposure of the developing foetus to a number of maternal factors which do not allow one to specifically define the role of thyroid hor...

journal_title：Biochimie

authors： Tata JR

更新日期：1999-04-01 00:00:00

abstract：:The lignan 8,8'-bis-(methylenedioxy)cinnamic acid (BMDCA) is a powerful competitive inhibitor (K1 = 2.0 microM) of the lignin peroxidase (LiP) from Phanerochaete chrysosporium and of the extracellular peroxidase of Phlebia radiata (I0.5 = 10 microM). BMDCA derivatives with the same double bond system also inhibited th...

journal_title：Biochimie

authors： Frías I,Trujillo JM,Romero J,Hernandez J,Pérez JA

更新日期：1995-01-01 00:00:00

abstract：:Accumulating evidences suggest that Herba epimedii has the potential benefits against osteoporosis. However, previous studies were focused on the crude extract, total flavonoids (TF) and icariin (ICA), and the detailed molecular mechanisms of action and structure-activity relationship (SAR) remain unclear. Herein we a...

journal_title：Biochimie

authors： Zhang D,Zhang J,Fong C,Yao X,Yang M

更新日期：2012-12-01 00:00:00

abstract：:(-)-Epigallocatechin-3-gallate (EGCG), a well-known chemopreventive factor, induces cancer cells undergoing apoptosis. Over the last several years, we have shown that the mitochondrial NADP(+)-dependent isocitrate dehydrogenase (IDPm) functions as an antioxidant and anti-apoptotic protein by supplying NADPH to antioxi...

journal_title：Biochimie

authors： Kil IS,Jung KH,Nam WS,Park JW

更新日期：2011-10-01 00:00:00

abstract：:In man the brain represents about 2% of the body weight, but contains 25% of the body's cholesterol. Cholesterol itself does not cross the blood-brain barrier and is synthesised in situ. Excess cholesterol from brain is exported in the form of oxysterols, or metabolised to steroids, which in contrast to cholesterol ca...

journal_title：Biochimie

authors： Wang Y,Karu K,Griffiths WJ

更新日期：2007-02-01 00:00:00

abstract：:Four full-length cDNAs encoding the precursors of four K(+)-toxin-like peptides (named BmKK(1), BmKK(2), BmKK(3) and BmmKK(4), respectively) were first isolated from a venom gland cDNA library of the Chinese scorpion Buthus martensii Karsch. The deduced precursors of BmKK(1), BmKK(2) and BmKK(3) are all made of 54 ami...

journal_title：Biochimie

authors： Zeng XC,Peng F,Luo F,Zhu SY,Liu H,Li WX

更新日期：2001-09-01 00:00:00

abstract：:Matrix metalloproteinases (MMPs) are a family of zinc endopeptidases that process growth factors, growth factor binding proteins, cell surface proteins, degrade extracellular matrix (ECM) components and thereby play a central role in tissue remodeling and tumor progression. Membrane-type matrix metalloproteinases (MT-...

journal_title：Biochimie

authors： Sounni NE,Noel A

更新日期：2005-03-01 00:00:00

abstract：:Due to their function as adapters in translation, tRNA molecules share a common structural organization in all kingdoms and organelles with ribosomal protein biosynthesis. A typical tRNA has a cloverleaf-like secondary structure, consisting of acceptor stem, D-arm, anticodon arm, a variable region, and T-arm, with an ...

journal_title：Biochimie

authors： Wende S,Platzer EG,Jühling F,Pütz J,Florentz C,Stadler PF,Mörl M

更新日期：2014-05-01 00:00:00

abstract：:Virtually all brain circuits are physically embedded in a three-dimensional matrix of fibers that release 5-hydroxytryptamine (5-HT, serotonin). The density of this matrix varies across brain regions and cortical laminae, and it is altered in some mental disorders, including Major Depressive Disorder and Autism Spectr...

journal_title：Biochimie

authors： Janušonis S,Detering N

更新日期：2019-06-01 00:00:00

abstract：PURPOSE:We evaluated the clinical relevance of microRNA-149 (miR-149) in neuroblastoma (NB) and its functional roles in regulating NB proliferation in vitro. METHODS:QRT-PCR was used to evaluate miR-149 expression in NB cell lines and primary NB tumors. Association between endogenous miR-149 expression in primary NB t...

journal_title：Biochimie

authors： Xu Y,Chen X,Lin L,Chen H,Yu S,Li D

更新日期：2017-08-01 00:00:00

abstract：:Human prophospholipase A2 was purified from pancreatic juice. The protein has a molecular weight of 14500 and a free N-terminal residue identified as aspartic acid (or asparagine). The amino acid composition was determined. Partial immunological identity has been obtained between human and porcine prophospholipase A2....

journal_title：Biochimie

authors： Grataroli R,De Caro A,Guy O,Amic J,Figarella C

更新日期：1981-08-01 00:00:00

abstract：:A new apolipoprotein has been identified in VHDL1 and in HDL. This protein is immunologically distinct from already isolated apoproteins. It was isolated by column chromatography on hydroxylapatite. In polyacrylamide gel electrophoresis, its mobility is very close to that of apo D. The amino acid composition differs f...

journal_title：Biochimie

authors： Ayrault-Jarrier M,Alix JF,Polonovski J

更新日期：1978-01-01 00:00:00

abstract：:Kex2 is a Ca2+-dependent serine protease from S. cerevisiae. Characterization of the substrate specificity of Kex2 is of particular interest because this protease serves as the prototype of a large family of eukaryotic subtilisin-related proprotein-processing proteases that cleave sites consisting of pairs or clusters...

journal_title：Biochimie

authors： Manfredi MA,Antunes AA,Jesus LO,Juliano MA,Juliano L,Judice WA

更新日期：2016-12-01 00:00:00

abstract：:The effects on intra- and extracellular pH of two polyenic derivatives of amphotericin B, N-fructosyl amphotericin B and N-fructosyl amphotericin B methyl-ester, were tested on HL-60 promyelocytic leukemia cells. Both derivatives raised the internal pH and reduced the external pH in weakly buffered medium. These resul...

journal_title：Biochimie

authors： Charretier E,Wietzerbin J

更新日期：1989-01-01 00:00:00

abstract：:RNA phosphodiester bonds can be cleaved by metal ions, of which Pb2+ is one of the most effective. It can cleave both generally and site-specifically, depending on the substrate and the conditions. In addition, metal ions are also known to cleave ester bonds between amino acid and the 3'-end of transfer RNA. Here we r...

journal_title：Biochimie

authors： Otzen DE,Barciszewski J,Clark BF

更新日期：1994-01-01 00:00:00

abstract：:Coenzymes are small organic molecules that supply a varied set of reactive groups to protein enzymes, thereby diversifying catalysis beyond the chemistries of amino acid sidechains. As RNA structures begin with a more limited chemical diversity than proteins, it seems likely that RNA enzymes would also use functional ...

journal_title：Biochimie

authors： Jadhav VR,Yarus M

更新日期：2002-09-01 00:00:00

abstract：:Pseudouridine [Ψ] is a frequent base modification in the ribosomal RNA [rRNA] and may be involved in the modulation of the conformational flexibility of rRNA helix-loop structures during protein synthesis. Helix 69 of 23S rRNA contains pseudouridines at the positions 1911, 1915 and 1917 which are formed by the helix 6...

journal_title：Biochimie

authors： Kipper K,Sild S,Hetényi C,Remme J,Liiv A

更新日期：2011-05-01 00:00:00

abstract：:A d-carbamoylase from Sinorhizobium morelens S-5 was purified and characterized. The enzyme was purified 189-fold to homogeneity with a yield of 19.1% by aqueous two-phase extraction and two steps of column chromatography. The enzyme is a homotetramer with a native molecular mass of 150 kDa and a subunit relative mole...

journal_title：Biochimie

authors： Wu S,Liu Y,Zhao G,Wang J,Sun W

更新日期：2006-03-01 00:00:00

abstract：:Sialidase catalyzes the removal of sialic acids from glycoconjugates. Recently, medaka sialidase Neu1 has been cloned and its enzymatic properties were investigated. Although enzymatic properties of this sialidase, such as optimal pH and substrate specificity, exhibits high similarity with human NEU1, Neu1 physiologic...

journal_title：Biochimie

authors： Ryuzono S,Takase R,Kamada Y,Ikenaga T,Chigwechokha PK,Komatsu M,Shiozaki K

更新日期：2017-04-01 00:00:00

abstract：:Several inositol isomers and in particular myo-inositol (MI) and D-chiro-inositol (DCI), were shown to possess insulin-mimetic properties and to be efficient in lowering post-prandial blood glucose. In addition, abnormalities in inositol metabolism are associated with insulin resistance and with long term microvascula...

journal_title：Biochimie

authors： Croze ML,Soulage CO

更新日期：2013-10-01 00:00:00

abstract：:Extracellular Hsp70 (eHsp70) can activate immune cells via Toll-like receptors (TLR) 2 and 4, and induce cytokine synthesis. The aim of this study was to explore inflammation-associated effects of eHsp70 alone and in combination with cigarette smoke extract (CSE) in primary bronchial epithelial cells. We assessed IL-6...

journal_title：Biochimie

authors： Hulina-Tomašković A,Heijink IH,Jonker MR,Somborac-Bačura A,Grdić Rajković M,Rumora L

更新日期：2019-01-01 00:00:00

abstract：:Epigenetic alterations are involved in every step of carcinogenesis. The development of chromatin-modifying agents (CMAs) has provided the ability to fight cancer by reversing these alterations. Currently, four CMAs have been approved for cancer treatment; two DNA demethylating agents and two deacetylase inhibitors. A...

journal_title：Biochimie

authors： Seidel C,Florean C,Schnekenburger M,Dicato M,Diederich M

更新日期：2012-11-01 00:00:00