Molecular diversity of "Candidatus Phytoplasma mali" strains associated with apple proliferation disease in Bulgarian germplasm collection.

Abstract:

:A quarantine organism, "Candidatus Phytoplasma mali," is the causal agent of apple proliferation, one of the most important apple diseases in Europe. The genetic diversity of this pathogen in Central and Southern Europe has already been reported; however, almost no data exists from Eastern Europe. In this study, "Ca. P. mali" strains, which were identified in 14 apple trees from the Bulgarian germplasm collection, were characterized by restriction fragment length polymorphism (RFLP) and sequence analysis of four genomic loci. In total, nine distinct genetic lineages were recognized based on the combination of the following detected RFLP profiles: two profiles for the 16S-23S rDNA region (16SrX-A2, -A3), four profiles for the secY gene (one previously known: secY(X)-A, and three new: secY-C, secY-D, secY-E), three profiles for the rpl22-rps3 genes (rpX-A, rpX-B, rpX-F), and one profile for the nitroreductase- and rhodanese-like gene (AT-1). Phylogenetic analysis of the Bulgarian and other European "Ca. P. mali" strains based on 16S-23S rRNA gene sequences confirmed RFLP grouping, regardless of the phytoplasma origin. In a phylogenetic tree based on the secY data, only German strains formed separate clade from the other strains. The tree based on rp genes did not correspond to RFLP profiles. Unexpectedly, when using nitroreductase and rhodanese-like gene sequences, the Bulgarian strains clustered separately from the other European strains. Apart from the identification of different "Ca. P. mali" strains, the paper also recommends the unification of the rpX-subgroup nomenclature to avoid future confusions. Both aims of this paper provide valuable tools to understand the epidemiology of this quarantine pathogen.

journal_title

Folia microbiologica

authors

Fránová J,Koloniuk I,Lenz O,Sakalieva D

doi

10.1007/s12223-018-0660-x

subject

Has Abstract

pub_date

2019-05-01 00:00:00

pages

373-382

issue

3

eissn

0015-5632

issn

1874-9356

pii

10.1007/s12223-018-0660-x

journal_volume

64

pub_type

杂志文章
  • Experimental model of Klebsiella infection in mice for studies on mechanisms of local immunity of the respiratory tract.

    abstract::An experimental model in white mice, infected with a mildly virulent strain of Klebsiella pneumoniae, was elaborated for studies on local immunity of the respiratory tract. Instillation of klebsiella into the supralaryngeal space of anaesthetised animals proved to be more suitable than the commonly used method of intr...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02879494

    authors: Libich J,Franĕk J,Hásková V

    更新日期:1983-01-01 00:00:00

  • Subinhibitory concentrations of organic ammonium salts: the effect on biological properties of Salmonella typhimurium.

    abstract::The effect of subinhibitory concentrations (subMICs) of new organic ammonium salts of four homologous series of alkylammonium bromides (32 compounds) was determined with respect to the induction of lysogenic strain prophage, influence of permeability reactions in a rabbit skin test and cytotoxic changes of monolayers ...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02815414

    authors: Majtánová L,Hostacká A,Majtán V

    更新日期:1995-01-01 00:00:00

  • Recommendations for cerebrospinal fluid analysis.

    abstract::Diseases of the central nervous system (CNS) mean for the human organism a potentially dangerous situation. An investigation of cerebrospinal fluid (CSF) provides important information about a character of CNS impairment in the decision-making diagnostic and therapeutic algorithm. The authors present a brief overview ...

    journal_title:Folia microbiologica

    pub_type: 杂志文章,评审

    doi:10.1007/s12223-018-0663-7

    authors: Hepnar D,Adam P,Žáková H,Krušina M,Kalvach P,Kasík J,Karpowicz I,Nasler J,Bechyně K,Fiala T,Mamiňák M,Malíková I,Mareš J,Šigut V,Tokár Z,Vránová J

    更新日期:2019-05-01 00:00:00

  • Industrial and agricultural wastes as substrates for laccase production by white-rot fungi.

    abstract::White-rot fungi, Coriolus versicolor and Funalia trogii, produced laccase on media with diluted olive-oil mill wastewater and vinasse. Addition of spent cotton stalks enhanced the laccase activity with a maximum after 12 d of cultivation. ...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02873591

    authors: Kahraman S,Yeşilada O

    更新日期:2001-01-01 00:00:00

  • Comparison of antimicrobial efficacy of a fixed dose combination of ceftazidime + sulbactam with ceftazidime and sulbactam alone against five bacteria.

    abstract::The efficacy of the agents was evaluated on the basis of minimum inhibitory concentration (MIC) and time-kill curve analysis in Pseudomonas aeruginosa, Staphylococcus aureus, Acinetobacter baumannii, Mycobacterium smegmatis, and Citrobacter braakii; MIC was found to be 0.5, 4.0, 0.015625, 0.0078125, and 0.0625 mg/L in...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-009-0054-1

    authors: Shrivastava SM,Shukla SK,Chaudhary M

    更新日期:2009-09-01 00:00:00

  • Ovine scrapie: priorities and importance.

    abstract::Ovine and caprine scrapie occupies a unique place among animal transmissive spongiform encephalopathies (TSE). It is an object of intensive biomedicinal, ecological and economical studies. Its causative agents are demonstrably associated with the development of TSE in farmed minks, goats and moufflons. Ovine strains o...

    journal_title:Folia microbiologica

    pub_type: 杂志文章,评审

    doi:10.1007/BF02818713

    authors: Novák M,Vrtiak OJ,Mikula I,Tkáciková L

    更新日期:2000-01-01 00:00:00

  • Efficacy of alkaloid (-)-corypalmine against spore germination of some fungi.

    abstract::Inhibition activity of the alkaloid (-)-corypalmine on spore germination of plant pathogenic and saprophytic fungi (Alternaria solani, A brassicicola, A. brassicae, A. melongenae, Curvularia pallescens, C. lunata, C. maculans, Curvularisa sp., Colletotrichum sp., Helminthosporium speciferum, H. frumentacei, H. pennise...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02817654

    authors: Maurya S,Srivastava JS,Jha RN,Pandey VB,Singh UP

    更新日期:2002-01-01 00:00:00

  • Cloning of endoglucanase genes from Cellulomonas biazotea into E. coli and S. cerevisiae using shuttle vector YEp24.

    abstract::We constructed a SmaI genomic library of Cellulomonas biazotea DNA in E. coli and in the S. cerevisiae shuttle vector, YEP 24. Three clone were identified that conferred the ability for E. coli or S. cerevisiae transformants to produce carboxymethylcellulase (CMCase). Cells transformed with these clones were compared ...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02814307

    authors: Parvez S,Rajoka MI,Fariha F,Malik KA

    更新日期:1994-01-01 00:00:00

  • Postantibiotic effects and postantibiotic sub-MIC effects of ciprofloxacin, pefloxacin and amikacin on the biological properties of Salmonella strains.

    abstract::The postantibiotic effect (PAE) and the postantibiotic sub-MIC effect (PASME) of ciprofloxacin, pefloxacin and amikacin were studied for Salmonella typhimurium and S. enteritidis strains. PAE was induced by 2 x and 4 x MIC of antibiotics studied for 0.5 h. After PAE and PASME their effect on prophage induction of a ly...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02816944

    authors: Majtán V,Majtánová L

    更新日期:1997-01-01 00:00:00

  • Properties of the cured oncogenic strain 37400 of Agrobacterium tumefaciens.

    abstract::The properties of the 37400 oncogenic strain of Agrobacterium tumefaciens are described. This strain was derived from the VI lysogenic strain originally isolated by Hamilton from a Zinnia elegans tumour. Strain 37400 has a number of properties which render it suitable for quantitative and genetic studies. It is cured ...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02876963

    authors: Bezdĕk M,Reich J,Tkadlecek L

    更新日期:1976-01-01 00:00:00

  • Production of 3-indolylacetic acid in root nodules and culture by a Rhizobium species isolated from root nodules of the leguminous pulse Phaseolus mungo.

    abstract::The root nodules of Phaseolus mungo (a herbaceous leguminous pulse) contained a high amount of 3-indolylacetic acid (IAA). A tryptophan pool present in the nodule might play the role of precursor for IAA production. From the root nodule a Rhizobium sp. was isolated. The symbiont produced a large amount of IAA (142 mic...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-008-0054-6

    authors: Ghosh S,Sengupta C,Maiti TK,Basu PS

    更新日期:2008-01-01 00:00:00

  • Effect of cultivation conditions on proteinase production by Lactobacillus murinus.

    abstract::Proteinase production by Lactobacillus murinus was influenced by temperature, glucose concentration, initial pH and nitrogen sources. Maximum proteinase production occurred at 45 degrees C, pH 6.6 and with 0.5% (W/V) glucose. Tryptone, peptone and gelatin inhibited it. ...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02877263

    authors: Strasser de Saad AM,Manca de Nadra MC,Pesce de Ruiz Holgado AA,Oliver G

    更新日期:1987-01-01 00:00:00

  • Ability of rumen protozoa Diploplastron affine to utilize β-glucans.

    abstract::The ability of the rumen ciliates to utilize β-glucans other than cellulose and xylan is currently being recognized. The objective of the present study was to characterize the ability of the ciliate Diploplastron affine to digest some pachyman, laminarin, pustulan, curdlan and lichean. The protozoa were isolated from ...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-012-0120-y

    authors: Belzecki G,Miltko R,Michalowski T

    更新日期:2012-07-01 00:00:00

  • Intracellular colonization of Rhododendron and Vaccinium roots by Cenococcum geophilum, Geomyces pannorum and Meliniomyces variabilis.

    abstract::Four in vitro experiments were set up to verify the colonization potential of ectomycorrhizal (EcM) Cenococcum geophilum FR. (strain CGE-4), saprotrophic Geomyces pannorum (LINK) SIGLER & CARMICHAEL (GPA-1) and a frequent root-associated, potentially ericoid mycorrhiza (ErM)-forming Meliniomyces variabilis Hambleton &...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02932096

    authors: Vohník M,Fendrych M,Albrechtová J,Vosátka M

    更新日期:2007-01-01 00:00:00

  • Ultrastructural study of the life cycle of Rickettsia slovaca, wild and standard type, cultivated in L929 and Vero cell lines.

    abstract::Ultrastructural changes induced by Rickettsia slovaca standard type (ST) and wild type (WT) were examined during their life cycle in L929 and Vero cells. R. slovaca invaded the cytoplasm of the host cell by phagocytosis on the 1st d p.i. Rickettsiae adhering to the cytoplasmic membrane were engulfed by cellular extens...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-009-0019-4

    authors: Boldis V,Strus J,Kocianová E,Tusek-Znidaric M,Stefanidesová K,Spitalská E

    更新日期:2009-01-01 00:00:00

  • Subcellular shifts of trimeric G-proteins following activation of baker's yeast by glucose.

    abstract::Addition of glucose to a resting cell suspension of the yeast Saccharomyces cerevisiae was accompanied by marked shifts of the G alpha-protein subunits from the plasma membrane to the cell interior. This process was rapid with half-times between < 10 and 20 s. The decrease of the plasma membrane pool of the Gi alpha/G...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02814427

    authors: Kotyk A,Ihnatovych I,Lapathitis G,Lamash N,Svoboda P

    更新日期:2001-01-01 00:00:00

  • Mosaic structure of the small cryptic plasmid pKST23 from Escherichia coli.

    abstract::Small plasmid pKST23 was isolated from sheep ruminal Escherichia coli population. Plasmid sequence was determined to be 2,779 bp in length and was found to have an overall 42 % of GC pairs. However, its sequence can be divided into two regions based on genetic composition and the GC content. It was found that the high...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-012-0124-7

    authors: Fecskeová L,Kovařík M,Javorský P,Pristaš P

    更新日期:2012-07-01 00:00:00

  • Purification and biochemical characterization of a mycelial alkaline phosphatase without DNAase activity produced by Aspergillus caespitosus.

    abstract::Biochemical properties of a termostable alkaline phosphatase obtained from the mycelium extract of A. caespitosus were described. The enzyme was purified 42-fold with 32% recovery by DEAE-cellulose and concanavalin A-Sepharose chromatography. The molar mass estimated by Sephacryl S-200 or by 7% SDS-PAGE was 138 kDa an...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02931303

    authors: Guimarães LH,Júnior AB,Jorge JA,Terenzi HF,Polizeli ML

    更新日期:2007-01-01 00:00:00

  • Characteristic distribution of immunoglobulin-containing cells in palatine tonsils.

    abstract::Using fluorescein-labelled antibodies against gamma, mu and alpha chains, Ig-containing cells in palatine tonsils were studied in 120 patients. The aim of this study was to determine the most frequently repeated typical findings as regards the numbers and localisation of these cells in tonsils and to confront the data...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02927432

    authors: Franĕk J,Kubín V,Novák V,Bedka J

    更新日期:1981-01-01 00:00:00

  • Extrusion of metabolites from baker's yeast during glucose-induced acidification.

    abstract::Extrusion of metabolites (glycerol, lactic, malic, and succinic acid) during the medium acidification caused by resting baker's yeast supplied with 200 mM glucose was studied under aerobic and anaerobic conditions and in the absence and presence of 14 mM KCl. The maximum levels of glycerol and of the sum of acids (abo...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02876611

    authors: Sigler K,Knotková A,Páca J,Wurst M

    更新日期:1980-01-01 00:00:00

  • Effect of pyruvate decarboxylase activity and of pyruvate concentration on the production of 1-hydroxy-1-phenylpropanone in Saccharomyces carlsbergensis.

    abstract::The assumption that the pyruvate decarboxylase activity of Saccharomyces carlsbergensis is the main limiting factor determining the formation rate and the total amount of d(--)-l-hydroxy-l-phenyl-propanone (phenylacetylcarbinol, PAC) produced was not confirmed. An increase of about 30% of the total amount of the PAC p...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02877396

    authors: Vojtísek V,Netrval J

    更新日期:1982-01-01 00:00:00

  • The effects of temperature, pH, and iron on biofilm formation by clinical versus environmental strains of Vibrio vulnificus.

    abstract::Due to the nature of Vibrio vulnificus infections (i.e., gastroenteritis and septicemia), only very few studies of a biofilm-associated form in this pathogen's life cycle have been conducted. We proposed that biofilm production by clinical strains of V. vulnificus would be higher than by environmental strains. Biofilm...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-019-00761-9

    authors: Çam S,Brinkmeyer R

    更新日期:2020-06-01 00:00:00

  • Degradation of organic nitrogenous wastes by a soil streptomycete.

    abstract::A soil streptomycete degraded hair, silk, wool, feather and leather which were collected from solid wastes. The organism was identified taxonomically and designated Streptomyces sp. A956. It degraded leather to the maximum extent and solubilized 35.9% of the total nitrogen, 2.32 mg of glycine equivalent amino nitrogen...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02927178

    authors: De S,Chandra AL

    更新日期:1979-01-01 00:00:00

  • Plant growth promotion and fungicidal activity in a siderophore-producing strain of Proteus sp.

    abstract::A proteus strain inhibited mycelial growth of Fusarium oxysporum in vitro. Seed bacterization showed significant plant growth promotion and Fusarium-wilt suppression activity of Phaseolus mungo in a gnotobiotic system. The culture filtrate of this strain exhibited three prominent bands in UV-VIS spectra between 300 an...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02818723

    authors: Barthakur M

    更新日期:2000-01-01 00:00:00

  • PCR-DGGE-based study of fecal microbial stability during the long-term chitosan supplementation of humans.

    abstract::A feeding study was performed to monitor the effect of chitosan intake on the fecal microbiota of ten healthy human subjects. Diversity of microflora was monitored during 8 weeks including 4 weeks of chitosan supplementations. Using denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene amplicons and...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-010-0057-y

    authors: Mrázek J,Koppová I,Kopecný J,Simůnek J,Fliegerová K

    更新日期:2010-07-01 00:00:00

  • Introducing a thermotolerant Gluconobacter japonicus strain, potentially useful for coenzyme Q10 production.

    abstract::In this report, Gluconobacter strains were screened for coenzyme Q10 (CoQ10) production. A thermotolerant strain, Gluconobacter japonicus FM10, was eventually employed for CoQ10 production optimization. To do so, a two-step optimization strategy was used. The first step focused on biomass increase and the second step ...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-018-0666-4

    authors: Moghadami F,Fooladi J,Hosseini R

    更新日期:2019-07-01 00:00:00

  • Assimilation spectrum of the yeast Candida utilis 49 used for producing fodder yeast from synthetic ethanol.

    abstract::Oxidizing and assimilating ability of the yeast Candida utilis 49 was tested with 21 different low-boiling organic compounds which come as components of raw synthetic ethanol. The highest yields of yeast dry weight were obtained with ethanol (72.0%), propanol (48.2%), ethyl acetate (43.4%) and acetic acid (34.2%). To ...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02876965

    authors: Sestáková M

    更新日期:1976-01-01 00:00:00

  • Biological properties of acmycin, a new polyene antibiotic.

    abstract::Acmycin, a new polyene antibiotic, was fungistatic at low and fungicidal at high concentrations. It produced malformations in conidia of Helminthosporium oryzae and Curvularia lunata and caused leakage of yeast cells. Acmycin was fairly nontoxic to germinating seeds. The intravenous LD50 was 300-400 and intraperitonea...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02879559

    authors: Chakrabarti S,Chandra AL

    更新日期:1983-01-01 00:00:00

  • Fatty acid analysis of Stenotrophomonas maltophilia clinical strains showing different susceptibility to antibiotics at 30 and 37 degrees C.

    abstract::Isolates of Stenotrophomonas maltophilia species display the feature "temperature-dependent susceptibility" (TDS) to antibiotics. Both 30TDS strains (at least 4 times lower value of minimum inhibitory concentration (MIC) of an antibiotic at 30 than at 37 degrees C) and 37TDS strains (at least 4 times lower value of MI...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02818682

    authors: Hejnar P,Chmela Z,Rypka M

    更新日期:2002-01-01 00:00:00

  • alpha-Mannosidase and mannanase of some wood-rotting fungi.

    abstract::Cultivation media from 11 wood-rotting fungi contained alpha-mannosidase and mannanase activity, alpha-Mannosidase was studied in more detail in Phellinus abietis and mannanase was studied more intimately in basidiomycetes Phellinus abietis, Trametes sanguinea and Pholiota aurivella. Suitable cultivation conditions an...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02876996

    authors: Zouchová Z,Kocourek J,Musílek V

    更新日期:1977-01-01 00:00:00