Abstract：
:We constructed a SmaI genomic library of Cellulomonas biazotea DNA in E. coli and in the S. cerevisiae shuttle vector, YEP 24. Three clone were identified that conferred the ability for E. coli or S. cerevisiae transformants to produce carboxymethylcellulase (CMCase). Cells transformed with these clones were compared with one another and with nontransformed cells for hyper-production of CMCase. In vivo and in vitro studies indicated that the CMCase genes were fully expressed and the enzyme activity was located extracellularly. The optimum pH and temperature for the CMCase thus cloned were pH 7 and 50 degrees C, respectively, as was the case for the donor.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Parvez S,Rajoka MI,Fariha F,Malik KAdoi
10.1007/BF02814307subject
Has Abstractpub_date
1994-01-01 00:00:00pages
251-4issue
4eissn
0015-5632issn
1874-9356journal_volume
39pub_type
杂志文章abstract：:A phosphate solubilizing fungus, Aspergillus awamori S29 was isolated from rhizoshpere of mungbean. The phosphate solubilizing activity of A. awamori S29 in liquid was 1,110 mg/L for tricalcium phosphate (TCP). The organism was able to solubilize various inorganic forms of phosphate at a wide range of temperatures. Am...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-012-0167-9
更新日期：2012-11-01 00:00:00
abstract：:Activity of purified alanylaminopeptidase of Pseudomonas sp. measured in the presence of the alanine derivative of 2-naphthoic acid (NA-Ala) is inhibited by 1,10-phenanthroline, EDTA, bestatin and amastatin; this finding supports the conclusion that this enzyme is a metallo-aminopeptidase. A decrease of its activity i...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02817643
更新日期：2002-01-01 00:00:00
abstract：:Genome projects produce a huge amount of sequence information. As a result, the focus of genomics research is turning toward deduction of functional information about newly discovered genes. Thus structural genomics paves the way for a new discipline called functional genomics by providing the information required for...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02931546
更新日期：2004-01-01 00:00:00
abstract：:Saccharomyces cerevisiae strain MW11 is a temperature-sensitive mutant which exports twenty times more proteins at 37 degrees C than parental or wild-type strains do. To understand the mechanism underlying the protein overexport in the mutant the possibility of an altered cell-wall structure leading to facilitated rel...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02908954
更新日期：2000-01-01 00:00:00
abstract：:In the present study, the cDNA sequences of Hsp70 and Hsp90 genes of Isaria farinosa (designated IFHSP70 and IFHSP90) were cloned and characterized using multiple techniques of molecular biology and bioinformatics. The genetic differentiation of the two genes was investigated among 10 geographically separated populati...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-018-00676-x
更新日期：2019-07-01 00:00:00
abstract：:Twenty-one healthy bottle-fed infants were screened monthly (1-4 months) for bifidobacteria in their stools. Bifidobacteria were detected by culture and isolates specified by PCR. Alternatively, direct PCR in undiluted fecal suspensions was carried out for detection of bifidobacteria under the cultural detection level...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931594
更新日期：2006-01-01 00:00:00
abstract：:Streptomyces cinnamonensis produces a new substance named AIB (for anti-isobutyrate) factor which, on a solid medium, efficiently counteracts toxic concentrations not only of isobutyrate but also of other salts of short-chain monocarboxylic acids. In the present study we demonstrate that the AIB factor activity is wid...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02891697
更新日期：1993-01-01 00:00:00
abstract：:The rosettes formed by mouse peritoneal macrophages or DCH-5 cells and TNP-erythrocytes coated with anti-TNP antibodies of different isotypes were inhibited to various extent by monosaccharides. The most effective inhibitors were N-acetylglucosamine, glucosamine, mannose and N-acetylneuraminic acid in 1-5 mmol/L conce...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814584
更新日期：1992-01-01 00:00:00
abstract：:Ultrastructural changes induced by Rickettsia slovaca standard type (ST) and wild type (WT) were examined during their life cycle in L929 and Vero cells. R. slovaca invaded the cytoplasm of the host cell by phagocytosis on the 1st d p.i. Rickettsiae adhering to the cytoplasmic membrane were engulfed by cellular extens...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-009-0019-4
更新日期：2009-01-01 00:00:00
abstract：:A wild-type strain of Candida albicans (S1, ATCC 10261) was used to obtain stable auxotrophic colony morphological mutants (mutant M5 producing only true hyphae and mutant M2 containing 90 % blastospores and 10 % pseudohyphae) by induced mutagenesis. A hybrid was produced by somatic hybridization between these 2 mutan...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931528
更新日期：2004-01-01 00:00:00
abstract：:The sorption ability of Candida utilis biomass for cadmium ions with accumulating competence of dried cells and cells in alginate was compared. After an optimization of process conditions (pH 5.5, biomass concentration 1 g/L and c0 50 mg/L), the cadmium sorption capacity of dried yeast biomass was perceptibly higher t...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931807
更新日期：2006-01-01 00:00:00
abstract：:The pathogenesis of bacillary dysentery can be studied at different levels of integration of the cellular components that constitute the colonic mucosal barrier. We considered the interaction of Shigella flexneri in three experimental systems that provide complementary information and a scheme of events occurring in h...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02818608
更新日期：1998-01-01 00:00:00
abstract：:Investigation of the dependence of cell yield of Candida boidinii in a chemostat and specific growth rate in a turbidostat on temperature was carried out using methanol as substrate. Each of the curves obtained had only one maximum, the optimum temperature for yield (Ys) and for specific growth rate (micron) being som...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02885600
更新日期：1977-01-01 00:00:00
abstract：:Effect of oxygen and metabolic substrates (glucose, ethanol) on the catalase activity of anaerobically grown Schizosaccharomyces pombe cells was assessed and compared with that of Saccharomyces cerevisiae in order to determine the catalase activity regulation in S. pombe. In contrast to S. cerevisiae, the total catala...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818575
更新日期：1998-01-01 00:00:00
abstract：:A method is presented for the successful decontamination of sparingly soluble pteridine derivatives by microwave irradiation. The method is nondestructive, rapid and effective in eliminating contamination. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814514
更新日期：1991-01-01 00:00:00
abstract：:The levels of anti-Candida antibodies were determined in experimental animals immunized with 4 different yeast doses (0.3, 0.6, 1.2, 2.4 x 10(8) CFU) at weekly intervals. After immunization (sampling intervals 1, 2, and 3 months), the intravenous blood was examined for the presence of serum anti-Candida antibodies. Af...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931340
更新日期：2003-01-01 00:00:00
abstract：:Altered cell signaling is the molecular basis for cell proliferation occurring in association with several gamma herpesvirus infections. Three gamma herpesviruses, namely EBV/HHV-4, KSHV/HHV-8 and the MHV-68 (and/or MHV-72) and their unusual cell-pirated gene products are discussed in this respect. The EBV, KSHV as we...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02931360
更新日期：2003-01-01 00:00:00
abstract：:The ability of surfactants obtained from three Lactobacillus acidophilus strains to inhibit Staphylococcus aureus and S. epidermidis biofilms was evaluated. Their influence was determined on bacterial initial adhesion, biofilm formation and dispersal using MTT-reduction assay, confocal laser scanning microscopy and im...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-008-0009-y
更新日期：2008-01-01 00:00:00
abstract：:Cord and newborn sera were found to possess a significantly decreased in vitro bactericidal activity to Escherichia coli as compared to sera of adult individuals. Hyperbilirubinaemia in neonates suffering from both Rh and ABO incompatibility results in a further significant impairment of the bactericidal activity of t...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02927298
更新日期：1979-01-01 00:00:00
abstract：:A total of 20 putative strains of Erwinia amylovora originating from 11 samples of host plants with symptoms of fire blight were analyzed in detail using commercial polyclonal antibodies in immunochemical tests. Fourteen strains reacted negatively in all tests; 6 strains reacted positively with a polyclonal antibody f...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931569
更新日期：2005-01-01 00:00:00
abstract：:In ostriches and pheasants, there is still limited information relating to staphylococci and their properties. Biogenic amines (BAs) are nitrogenous low-molecular-weight substances with biological functions in animals, plants and microorganisms. In this study, we focused on BA production by targeted faecal staphylococ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-017-0492-0
更新日期：2017-05-01 00:00:00
abstract：:The ability of the rumen ciliates to utilize β-glucans other than cellulose and xylan is currently being recognized. The objective of the present study was to characterize the ability of the ciliate Diploplastron affine to digest some pachyman, laminarin, pustulan, curdlan and lichean. The protozoa were isolated from ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-012-0120-y
更新日期：2012-07-01 00:00:00
abstract：:The postantibiotic effect (PAE) and the postantibiotic sub-MIC effect (PASME) of ciprofloxacin, pefloxacin and amikacin were studied for Salmonella typhimurium and S. enteritidis strains. PAE was induced by 2 x and 4 x MIC of antibiotics studied for 0.5 h. After PAE and PASME their effect on prophage induction of a ly...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02816944
更新日期：1997-01-01 00:00:00
abstract：:beta-Glucosidase from the fungus Thermoascus aurantiacus grown on semi-solid fermentation medium (using ground corncob as substrate) was partially purified in 5 steps--ultrafiltration, ethanol precipitation, gel filtration and 2 anion exchange chromatography runs, and characterized. After the first anion exchange chro...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818672
更新日期：2002-01-01 00:00:00
abstract：:Although humic acids (HA) are involved in many biological processes in soils and thus their ecological importance has received much attention, the degradative pathways and corresponding catalytic genes underlying the HA degradation by bacteria remain unclear. To unveil those uncertainties, we analyzed transcriptomes e...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-017-0573-0
更新日期：2018-05-01 00:00:00
abstract：:Microorganisms that cause chronic infections exist predominantly as surface-attached stable communities known as biofilms. Microbial cells in biofilms are highly resistant to conventional antibiotics and other forms of antimicrobial treatment; therefore, modern medicine tries to develop new drugs that exhibit anti-bio...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-018-0633-0
更新日期：2019-01-01 00:00:00
abstract：:A series of experiments were performed with P. aeruginosa to demonstrate which of the biochemical mechanisms are responsible for the resistance to the beta-lactam antibiotics. The constitutive beta-lactamase was isolated and characterized for the strain used as type OXA whose pI was 7.1, with a molar mass of 49 kg/mol...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02877216
更新日期：1987-01-01 00:00:00
abstract：:Mathematical expressions were derived for the dilution rate and concentration of algae determining the maximum biomass production in a semicontinuous algal culture. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02926655
更新日期：1979-01-01 00:00:00
abstract：:Small plasmid pKST23 was isolated from sheep ruminal Escherichia coli population. Plasmid sequence was determined to be 2,779 bp in length and was found to have an overall 42 % of GC pairs. However, its sequence can be divided into two regions based on genetic composition and the GC content. It was found that the high...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-012-0124-7
更新日期：2012-07-01 00:00:00
abstract：:Physiological and biochemical properties were tested in 45 isolates of Aspergillus egyptiacus (16 isolates), Emericella nidulans (16) and Aspergillus versicolor (13). The three fungal species exhibited common and similar features. The big similarity between A. egyptiacus and E. nidulans was greater than between A. egy...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814449
更新日期：1994-01-01 00:00:00
