The influence of Lactobacillus acidophilus-derived surfactants on staphylococcal adhesion and biofilm formation.

abstract：:An economic method for a rapid estimation of the number of copies of plasmid R6K delta 1 in E. coli cells using lysis of the cells directly on the agarose gel and electrophoretic separation of radioactively labelled plasmid and chromosomal DNA's is described. The method, particularly useful for screening purposes, per...

journal_title：Folia microbiologica

authors： Hochmannová J,Nesvera J

更新日期：1982-01-01 00:00:00

abstract：:In yeast, the STB5 gene encodes a transcriptional factor belonging to binuclear cluster class (Zn2Cys6) of transcriptional regulators specific to ascomycetes. In this study, we prepared the Kluyveromyces lactis stb5Δ strain and assessed its responses to different stresses. We showed that KlSTB5 gene is able to complem...

journal_title：Folia microbiologica

authors： Bencova A,Konecna A,Toth Hervay N,Gbelska Y

更新日期：2019-07-01 00:00:00

abstract：:Strains of genus Citrobacter (152 isolates from 1950 to 1988 deposited in the Czech National Collection of Type Cultures, Prague) were re-classified using biological and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) methods. One-hundred thirty-six strains (ca. 90 %) were i...

journal_title：Folia microbiologica

authors： Kolínská R,Spanělová P,Dřevínek M,Hrabák J,Zemličková H

更新日期：2015-01-01 00:00:00

abstract：:One-hundred patients with candidosis of the skin, mucous membranes and nail plates (confirmed by cultivation) were examined. Topical or systemic antimycotic treatment was successful in 58 patients. After a complete evaluation, 42 patients were found to suffer from factors supporting candidosis--diabetes mellitus (12),...

journal_title：Folia microbiologica

authors： Jautová J,Baloghová J,Dorko E,Pilipcinec E,Svický E,Danko J,Tkáciková L

更新日期：2001-01-01 00:00:00

abstract：:Nalidixic acid was used for describing more accurately the terminal replication region of the Mycobacterium phlei chromosome. Cell division in synchronized cultures was not sensitive to this acid any more between 185-190 min, i.e. about 10 min after replication of the ser gene, the last of 24 genes of the replication ...

journal_title：Folia microbiologica

authors： Konícek J,Konícková-Radochová M,Cáslavská J,Wolf A

更新日期：1981-01-01 00:00:00

abstract：:In this report, Gluconobacter strains were screened for coenzyme Q10 (CoQ10) production. A thermotolerant strain, Gluconobacter japonicus FM10, was eventually employed for CoQ10 production optimization. To do so, a two-step optimization strategy was used. The first step focused on biomass increase and the second step ...

journal_title：Folia microbiologica

authors： Moghadami F,Fooladi J,Hosseini R

更新日期：2019-07-01 00:00:00

abstract：:Brefeldin A has been recently characterized to act as an inhibitor of intracellular protein export with profound effects on the structure and function of the Golgi apparatus in animal cells. Manifold activities of the antibiotic (under different names) published in the 1960's and 1970's are reviewed: effects on fungal...

journal_title：Folia microbiologica

authors： Betina V

更新日期：1992-01-01 00:00:00

abstract：:The level of an antibiotic capable of inhibiting the etiological agent at the site of infection is an essential prerequisite for successful antibiotic therapy. In some cases, locally applied antibiotics may compensate for limitations of systemic administration and shorten systemic therapy. We aimed at verifying to wha...

journal_title：Folia microbiologica

authors： Melicherčík P,Jahoda D,Nyč O,Klapková E,Barták V,Landor I,Pokorný D,Judl T,Sosna A

更新日期：2012-09-01 00:00:00

abstract：:Strains of Rhodospordium torloides produced two peptides into the culture filtrate. Peptide I contained 6 amino acids and peptide II contained 5 amino acids. The peptides however, are, not homologous to the alpha-factor, and become assimilated at a later phase of growth. ...

journal_title：Folia microbiologica

authors： Chatterjee SP,Birnbaum D

更新日期：1980-01-01 00:00:00

abstract：:In the present study, the cDNA sequences of Hsp70 and Hsp90 genes of Isaria farinosa (designated IFHSP70 and IFHSP90) were cloned and characterized using multiple techniques of molecular biology and bioinformatics. The genetic differentiation of the two genes was investigated among 10 geographically separated populati...

journal_title：Folia microbiologica

authors： He L,Yu H,Dai Y,Wang Y

更新日期：2019-07-01 00:00:00

abstract：:The aim of the study was to compare the phenotype of lymphocyte subpopulations of the GALT (gut-associated lymphatic tissue) in germfree (GF) and conventionally (CV) reared rats, i.e. to analyze the effect of microbial colonization on the development of intestinal lymphocyte subsets. Surface marker characteristics wer...

journal_title：Folia microbiologica

authors： Stĕpánková R,Sinkora J,Hudcovic T,Kozáková H,Tlaskalová-Hogenová H

更新日期：1998-01-01 00:00:00

abstract：:Twenty-one healthy bottle-fed infants were screened monthly (1-4 months) for bifidobacteria in their stools. Bifidobacteria were detected by culture and isolates specified by PCR. Alternatively, direct PCR in undiluted fecal suspensions was carried out for detection of bifidobacteria under the cultural detection level...

journal_title：Folia microbiologica

authors： Mullié C,Romond MB,Izard D

更新日期：2006-01-01 00:00:00

abstract：:Biotechnological conversion of low-cost agro-industrial by-products, such as industrial waste or terpenes from the distillation of essential oils from plants into more valuable oxygenated derivatives, can be achieved by using microbial cells or enzymes. In Argentina, the essential oil industry produces several tons of...

journal_title：Folia microbiologica

authors： Omarini A,Dambolena JS,Lucini E,Jaramillo Mejía S,Albertó E,Zygadlo JA

更新日期：2016-03-01 00:00:00

abstract：:Proteinase production by Lactobacillus murinus was influenced by temperature, glucose concentration, initial pH and nitrogen sources. Maximum proteinase production occurred at 45 degrees C, pH 6.6 and with 0.5% (W/V) glucose. Tryptone, peptone and gelatin inhibited it. ...

journal_title：Folia microbiologica

authors： Strasser de Saad AM,Manca de Nadra MC,Pesce de Ruiz Holgado AA,Oliver G

更新日期：1987-01-01 00:00:00

abstract：:Mice (strains Balb/c and A/J) received an intravenous infusion of bilirubin for a 1 d period. The infusion was delivered at various phases of the primary reaction; the degree of the immune response was expressed as the number of antibody-forming cells against sheep erythrocytes. Bilirubin infusion during both the indu...

journal_title：Folia microbiologica

authors： Síma P,Malá J,Miler I,Hodr R,Truxová E

更新日期：1980-01-01 00:00:00

abstract：:A new simple method used to eliminate polysaccharides that cause problems during DNA isolation was established for 6 different white-rot fungi using 1% hexadecyltrimethylammonium bromide (CTAB) as wash buffer and followed by centrifugation. Variation in the DNA yield and quality was ascertained using precipitating age...

journal_title：Folia microbiologica

authors： Kuhad RC,Kapoor RK,Lal R

更新日期：2004-01-01 00:00:00

abstract：:The possibility of utilizing derivatives of 4-methylumbelliferone (4-MUBF) for the determination of activity of enzymes from the group of hydrolyses was tested in 20 strains of 12 species of mycobacteria. The method proved to be suitable especially for determination of arylsulphatase, beta-D-galactose and acid phospha...

journal_title：Folia microbiologica

authors： Slosárek M

更新日期：1980-01-01 00:00:00

abstract：:Murine herpesvirus 68 (MHV-68) is a natural pathogen that infects murid rodents, which serves as hosts for Ixodes ricinus ticks. For the first time, MHV-68 was detected in immature I. ricinus ticks feeding on Lacerta viridis lizards trapped in Slovakia, which supports the idea that ticks can acquire the virus from fee...

journal_title：Folia microbiologica

authors： Kúdelová M,Jánošová M,Belvončíková P

更新日期：2018-07-01 00:00:00

abstract：:A wild-type strain of Candida albicans (S1, ATCC 10261) was used to obtain stable auxotrophic colony morphological mutants (mutant M5 producing only true hyphae and mutant M2 containing 90 % blastospores and 10 % pseudohyphae) by induced mutagenesis. A hybrid was produced by somatic hybridization between these 2 mutan...

journal_title：Folia microbiologica

authors： Novák A,Vágvölgyi C,Emody L,Pesti M

更新日期：2004-01-01 00:00:00

abstract：:The autofluorescence (primary fluorescence, AF) of agar cultures of the brown-rot fungus Piptoporus betulinus was investigated in Zeiss Jenalumar and Nikon Eclipse 8201 fluorescence microscopes at various excitations. The strongest AF of hyphae was found in minimal medium with glucose, where the hyphae exhibited green...

journal_title：Folia microbiologica

authors： Zižka Z,Vêtrovský T,Gabriel J

更新日期：2010-11-01 00:00:00

abstract：:We report a novel application of calcein-acetomethyl ester in flow cytometry for rapid estimation of the number of G+-bacteria in rodent feces (Apodemus sylvaticus and Mus sp.f. muridae). We also use the combined application of flow cytometry and Syto-13 or Sypro Orange staining to count rapidly the total bacterial po...

journal_title：Folia microbiologica

authors： Moragues M,Comas-Riu J,Vives-Rego J

更新日期：2004-01-01 00:00:00

abstract：:Pseudomonas aeruginosa is an opportunistic pathogen that causes serious infections, especially in patients with immunodeficiency. It exhibits multiple mechanisms of resistance, including efflux pumps, antibiotic modifying enzymes and limited membrane permeability. The primary reason for the development of novel therap...

journal_title：Folia microbiologica

authors： Kwiatek M,Mizak L,Parasion S,Gryko R,Olender A,Niemcewicz M

更新日期：2015-01-01 00:00:00

abstract：:Three types of heterogeneous preparations and four types of preparations of polysaccharide nature were obtained in studies aimed at the isolation of active compounds from Aspergillus flavus conidia bearing their biological stimulatory activity. Extraction with trichloroacetic acid at 0 degrees C yielded a preparation ...

journal_title：Folia microbiologica

authors： Budínská O,Franĕk J,Kubín V,Fiedorov BA,Stasilevitch ZK

更新日期：1981-01-01 00:00:00

abstract：:Bifidobacteria (246 strains in total) were isolated from rectal samples of infants and adult humans and animals, and from intestinal samples of calves. Twenty-five strains grew well on mucin: 20 from infants, two from adults, and three from goatlings. Poor or no growth on mucin was observed in 156 bifidobacterial stra...

journal_title：Folia microbiologica

authors： Killer J,Marounek M

更新日期：2011-03-01 00:00:00

abstract：:Killer strains of Saccharomyces cerevisiae producing killer toxin K1 kill sensitive cells but are resistant to their own toxin. It is assumed that in the producer, an effective interaction between the external toxin and its plasma membrane receptor or the final effector is not possible on the grounds of a conformation...

journal_title：Folia microbiologica

authors： Valis K,Masek T,Novotná D,Pospísek M,Janderová B

更新日期：2006-01-01 00:00:00

abstract：:Cyanobacteria are one of the most successful and oldest forms of life that are present on Earth. They are prokaryotic photoautotrophic microorganisms that colonize so diverse environments as soil, seawater, and freshwater, but also stones, plants, or extreme habitats such as snow and ice as well as hot springs. This d...

journal_title：Folia microbiologica

authors： Řezanka T,Palyzová A,Sigler K

更新日期：2018-09-01 00:00:00

abstract：:Saccharomyces cerevisiae strain MW11 is a temperature-sensitive mutant which exports twenty times more proteins at 37 degrees C than parental or wild-type strains do. To understand the mechanism underlying the protein overexport in the mutant the possibility of an altered cell-wall structure leading to facilitated rel...

journal_title：Folia microbiologica

authors： Alexieva KI,Venkov PV

更新日期：2000-01-01 00:00:00

abstract：:Glycerol-based medium (BM) with and without the addition of 1 g/L ascorbic acid (Asc) and/or 100 mg/L (+/-)-catechin (Cat) was tested for the storage of three commercial wine yeasts at -20 degrees C. The medium supplemented with Asc was also used to store 706 strains to verify the maintenance of the liquid state. A de...

journal_title：Folia microbiologica

authors： Sidari R,Caridi A

更新日期：2009-01-01 00:00:00

abstract：:Procedures to introduce point mutations, restriction sites and insert or delete DNA fragments are very important tools to study protein function. We describe here two-step PCR-based method for generating single or multiple mutations, insertions and deletions in a small region of the sequence. In the first step, a uniq...

journal_title：Folia microbiologica

authors： Adamec J,Kalousek F

更新日期：1999-01-01 00:00:00

abstract：:The plasma membrane and mitochondria of bottom fermenting brewer's yeast obtained as a by-product of industrial beer production were isolated and the lipid fraction was analyzed. The phospholipid content accounted for 78 mg/g protein in the plasma membrane and 59 mg/g protein in the mitochondria. Major phospholipids i...

journal_title：Folia microbiologica

authors： Blagović B,Rupcić J,Mesarić M,Marić V

更新日期：2005-01-01 00:00:00