Abstract:
:We report a novel application of calcein-acetomethyl ester in flow cytometry for rapid estimation of the number of G+-bacteria in rodent feces (Apodemus sylvaticus and Mus sp.f. muridae). We also use the combined application of flow cytometry and Syto-13 or Sypro Orange staining to count rapidly the total bacterial population and to describe bacterial subpopulations in the intestine.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Moragues M,Comas-Riu J,Vives-Rego Jdoi
10.1007/BF02931538keywords:
subject
Has Abstractpub_date
2004-01-01 00:00:00pages
587-90issue
5eissn
0015-5632issn
1874-9356journal_volume
49pub_type
杂志文章abstract::Using fermentation with a selected strain of Acetomonas oxydans it was possible to convert 2-deoxy-2-fluro-D-glucitol to 5-deoxy-5-fluoro-L-sorbose, in agreement with Bertrand's and Hudson's rule. The last-named compound was isolated in a yield of 88%. Both compounds were little toxic against Acetomonas oxydans. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02877659
更新日期:1977-01-01 00:00:00
abstract::Two classes of newly synthesized amphiphilic compounds, phenolic antioxidants ("phenolics") and N-oxides exert in vivo antioxidant effects on live S. cerevisiae cells. Both groups have low toxicity, phenolics being more toxic than N-oxides and compounds with a longer alkyl chain having higher toxicity than those with ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02932187
更新日期:2007-01-01 00:00:00
abstract::Cardiolipin synthesis was studied during the aerobic synchronous growth of aerobically grown yeast Saccharomyces cerevisiae. The time course of the synthesis was stepwise and the rise in cardiolipin level in cells coincided in time with the increase in cytochrome oxidase activity. This finding supports the notion of d...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876990
更新日期:1977-01-01 00:00:00
abstract::Cultivation media from 11 wood-rotting fungi contained alpha-mannosidase and mannanase activity, alpha-Mannosidase was studied in more detail in Phellinus abietis and mannanase was studied more intimately in basidiomycetes Phellinus abietis, Trametes sanguinea and Pholiota aurivella. Suitable cultivation conditions an...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876996
更新日期:1977-01-01 00:00:00
abstract::Inducible pigmentation changes were observed in pigmented strains of Brevibacterium sp. M27 and B. flavum treated with N-methyl-N'-nitro-N-nitrosoguanidine. The highest frequency of induction was reached already at a survival of 30-40% with the maximal yield of 6-10%. As compared with the initial yellow colour, three ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02928007
更新日期:1988-01-01 00:00:00
abstract::The ability of the rumen ciliates to utilize β-glucans other than cellulose and xylan is currently being recognized. The objective of the present study was to characterize the ability of the ciliate Diploplastron affine to digest some pachyman, laminarin, pustulan, curdlan and lichean. The protozoa were isolated from ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-012-0120-y
更新日期:2012-07-01 00:00:00
abstract::Altered cell signaling is the molecular basis for cell proliferation occurring in association with several gamma herpesvirus infections. Three gamma herpesviruses, namely EBV/HHV-4, KSHV/HHV-8 and the MHV-68 (and/or MHV-72) and their unusual cell-pirated gene products are discussed in this respect. The EBV, KSHV as we...
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/BF02931360
更新日期:2003-01-01 00:00:00
abstract::There is a growing interest in the use of bioinoculants to assist mineral fertilizers in improving crop production and yield. Azotobacter and Pseudomonas are two agriculturally relevant strains of bacteria which have been established as efficient bioinoculants. An experiment involving addition of graded concentrations...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-017-0495-x
更新日期:2017-05-01 00:00:00
abstract::Several amino acids affected the amount of bacitracin produced by Bacillus licheniformis ATCC 10716 and the high-yielding mutant B. licheniformis AL. The possibility of an amino acid control mechanism for bacitracin biosynthesis is discussed. The two strains showed a different response to the addition of some amino ac...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02926454
更新日期:1979-01-01 00:00:00
abstract::Cultures of 33 basidiomycete strains out of 35 tested were viable with unchanged characteristics after four years of maintenance on perlite in cryovials. These cultures can be a good substitute for agar cultures in long-term maintenance of fungi. For comparison, the storage under oil was evaluated but it turned out to...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-008-0084-0
更新日期:2008-01-01 00:00:00
abstract::Vibrio el tor cells contain a constitutive reductase enzyme which converts nitrofurantoin to an active principle that is responsible for the observed antibacterial activity of the drug. Acquisition of resistance of this strain towards nitrofurantoin is associated with the loss of this reductase. This enzyme is located...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02927181
更新日期:1979-01-01 00:00:00
abstract::The possibility of utilizing derivatives of 4-methylumbelliferone (4-MUBF) for the determination of activity of enzymes from the group of hydrolyses was tested in 20 strains of 12 species of mycobacteria. The method proved to be suitable especially for determination of arylsulphatase, beta-D-galactose and acid phospha...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876698
更新日期:1980-01-01 00:00:00
abstract::The structural gene coding for YompC has been identified in the genome of a pathogenic strain of Yersinia enterocolitica O:9, and was subsequently cloned and sequenced. Detailed alignment of the deduced amino acid sequence showed that YompC is a member of the OmpC porin family with the highest degree of homology to Kl...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02932142
更新日期:2007-01-01 00:00:00
abstract::Studies on the production of extracellular cellulase in two aquatic hyphomycetes Lunulospora curvula and Flagellospora penicillioides have shown that several factors such as carbon source, nitrogen source, pH and temperature affect the production of the enzyme. Experiments have shown that carboxymethyl cellulose is th...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814357
更新日期:1991-01-01 00:00:00
abstract::A long-living artificial tripartite symbiosis involving a green alga (Chlamydomonas), a bacterium (Azotobacter) and a fungus (Alternaria) was established on carbon- and nitrogen-free medium. The basis of the interdependence is the complementation of photosynthetic CO2 assimilation and atmospheric nitrogen fixation. Gr...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-010-0067-9
更新日期:2010-07-01 00:00:00
abstract::This review discusses methods for the single-chain antibody fragment ($cFv) generation and scFv expression systems, and describes potential applications of scFv in the therapy of viral diseases and cancer, with emphasis on intracellularly expressed scFvs (intrabodies), application of scFvs in detection and diagnostics...
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/BF02993480
更新日期:2003-01-01 00:00:00
abstract::Inactivation of seven strains derived from Escherichia coli B differing in their capacity to repair damage to their DNA (exc, pol, rec) after irradiation with far (254 nm) and middle and near (300 to 380 and 320--400 nm) UV light was investigated. The same bacterial strains were also used as hosts for the UV-irradiate...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876690
更新日期:1980-01-01 00:00:00
abstract::Streptomyces cinnamonensis produces a new substance named AIB (for anti-isobutyrate) factor which, on a solid medium, efficiently counteracts toxic concentrations not only of isobutyrate but also of other salts of short-chain monocarboxylic acids. In the present study we demonstrate that the AIB factor activity is wid...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02891697
更新日期:1993-01-01 00:00:00
abstract::Lactobacillus bulgaricus 59 and L. casei RTS and their respective nitrosoguanidine-induced mutants were estimated for titratable and volatile acidities, production of biacetyl and acetoin and for proteolytic activity. Both the mutants showed a marked increase in proteolytic activity as compared to the parents. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02897216
更新日期:1980-01-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) viral protein R (Vpr) exerts multiple effects on viral and host cellular activities during infection, including induction of the cell cycle G2 arrest, and cell death in both human cells and the fission yeast Schizosaccharomyces pombe. We show that treament of exponential-pha...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931584
更新日期:2006-01-01 00:00:00
abstract::Distribution of microtubules and F-actin in aerobically growing cells of Dipodascus magnusii, belonging to the class Saccharomycetes was analyzed using immunofluorescence microscopy and labeling with rhodamine-tagged phalloidin. A conspicuous system of permanent cytoplasmic microtubules was observed in association wit...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02930952
更新日期:2003-01-01 00:00:00
abstract::3-(1,1-Dioxadibenzothien-4-oyl)acrylic acid (1) was condensed with compounds containing active methylene groups under Michael reaction conditions to furnish the Michael adducts (lactones 2a-c, lactams 3a-c, ketones 4a,b). The behavior of these adducts toward the action of hydrazine hydrate were investigated. The compo...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02817670
更新日期:2002-01-01 00:00:00
abstract::A feeding study was performed to monitor the effect of chitosan intake on the fecal microbiota of ten healthy human subjects. Diversity of microflora was monitored during 8 weeks including 4 weeks of chitosan supplementations. Using denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene amplicons and...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-010-0057-y
更新日期:2010-07-01 00:00:00
abstract::Candidiases, infections caused by germination forms of the Candida fungus, represent a heterogeneous group of diseases from systemic infection, through mucocutaneous form, to vulvovaginal form. Although caused by one organism, each form is controlled by distinct host immune mechanisms. Phagocytosis by polymorphonuclea...
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/BF02931313
更新日期:2007-01-01 00:00:00
abstract::Blastoschizomyces capitatus is an uncommon, opportunistic pathogenic fungus, which causes invasive and disseminated infections. This microorganism is normally present in both environmental and normal human flora. Within a host, B. capitatus is able to grow in both unicellular yeast and multicellular filamentous growth...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-013-0273-3
更新日期:2014-03-01 00:00:00
abstract::Numerous antibodies with a known mechanism of action are utilized as possible means for studying morphogenesis and differentiation. Inhibitors of biosynthesis of nucleic acids and proteins, compounds intervening with the synthesis and/or function of cell walls and membranes or compounds influencing the energy metaboli...
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/BF02897219
更新日期:1980-01-01 00:00:00
abstract::Two Bacillus cereus feather-degrading strains (23/1 and 6/2) were transformed using a recombinant plasmid p5.2 carrying the alkaline proteinase gene (aprE). A high level of the aprE gene expression was observed when the recombinant strains were grown on sporulation medium. The expression of the aprE gene proceeded dur...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-010-0004-y
更新日期:2010-01-01 00:00:00
abstract::The synthesis of exocellular proteinase decreases with increasing concentration of amino acids in the growth medium. After removal of amino acids the enzyme synthesis is gradually restored to normal values. The presence of inhibitors of transcription (actinomycin D) or translation (chloramphenicol) blocks the restorat...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02875957
更新日期:1984-01-01 00:00:00
abstract::Bacterial metabolites with communicative functions could provide protection against stress conditions to members of the same species. Yet, information remains limited about protection provided by metabolites in Bacillus cereus and inter-species. This study investigated the effect of extracellular compounds derived fro...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-016-0487-2
更新日期:2017-05-01 00:00:00
abstract::This is the first report describing precipitation of bacteriophage particles with zinc chloride as a method of choice to isolate infectious lytic bacteriophages against Pectobacterium spp. and Dickeya spp. from environmental samples. The isolated bacteriophages are ready to use to study various (ecological) aspects of...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-015-0411-1
更新日期:2016-01-01 00:00:00