Abstract：
:The ability of the rumen ciliates to utilize β-glucans other than cellulose and xylan is currently being recognized. The objective of the present study was to characterize the ability of the ciliate Diploplastron affine to digest some pachyman, laminarin, pustulan, curdlan and lichean. The protozoa were isolated from the rumen of sheep and either grown in vitro or inoculated into the rumen of ciliate-free sheep and maintained in natural conditions. In vitro culture studies showed that the enrichment of culture medium with the examined saccharides results in an increase in the number of ciliates in comparison to the control cultures. The increase was over 36 and 15 % when the growth medium was supplemented with pachyman (1,3-β-glucan) and pustulan (1,6-β-glucan), respectively. A positive correlation was also found between the population density of ciliates and the dose of saccharide supplemented to the growth medium. Enzyme studies were performed using the crude enzyme preparation obtained from ciliates treated with antibiotics. The ability of ciliates to digest the examined β-glucans was tested by the quantification of reducing sugars released from the mentioned substrates during the incubation with crude enzyme preparation. The results showed that D. affine ciliates were able to digest both of them. The mean degradation rate varied between 6.7 and 28.2 μmol/L glucose per mg protein per h for pustulan and lichean, respectively, whereas the digestion velocity was the highest at 5.0-5.5 pH and 45-50°C.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Belzecki G,Miltko R,Michalowski Tdoi
10.1007/s12223-012-0120-ysubject
Has Abstractpub_date
2012-07-01 00:00:00pages
259-62issue
4eissn
0015-5632issn
1874-9356journal_volume
57pub_type
杂志文章abstract：:Overpopulation of domestic pigeons is considered to be one of the major problems of urban centers, as these birds are responsible for the dissemination of relevant pathogens to animal and human health. The aim of this study was to detect potentially pathogenic Escherichia coli and Salmonella spp. in domestic pigeons c...
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更新日期：2001-01-01 00:00:00
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journal_title：Folia microbiologica
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doi：10.1007/BF02814645
更新日期：1994-01-01 00:00:00
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doi：10.1007/BF02877677
更新日期：1977-01-01 00:00:00
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更新日期：2004-01-01 00:00:00
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doi：10.1007/BF02816234
更新日期：1999-01-01 00:00:00
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更新日期：2001-01-01 00:00:00
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更新日期：1994-01-01 00:00:00
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更新日期：2010-07-01 00:00:00
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doi：10.1007/BF02877669
更新日期：1977-01-01 00:00:00
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更新日期：2018-11-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/BF02814202
更新日期：1995-01-01 00:00:00
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journal_title：Folia microbiologica
pub_type： 杂志文章
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更新日期：2007-01-01 00:00:00
