Indigenous plasmids in a production line of strains for penicillin G acylase derived from Escherichia coli W.

Abstract:

:Three indigenous plasmids designated pRK1, pRK2 and pRK3 were identified among producers of penicillin G acylase (PGA) derived from the strain Escherichia coli W ATCC 9637. Their size and copy number (CN) in E. coli W were determined (kb; CN): pRK1 (80; 3.4), pRK2 (5.1; 71), and pRK3 (4.8; 13.7). Strain E. coli RE2 harboring these plasmids was used for selection of strains with reduced number of plasmids: the strain RE3 without plasmid pRK1 and the plasmid-less strain cERE3 were isolated. Indigenous plasmids did not code for the resistance determinants against 23 antibiotics and 10 heavy metals.

journal_title

Folia microbiologica

authors

Sobotková L,Grafková J,Stĕpánek V,Vacík T,Maresová H,Kyslík P

doi

10.1007/BF02818544

keywords:

subject

Has Abstract

pub_date

1999-01-01 00:00:00

pages

263-6

issue

3

eissn

0015-5632

issn

1874-9356

journal_volume

44

pub_type

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