Abstract:
:Three indigenous plasmids designated pRK1, pRK2 and pRK3 were identified among producers of penicillin G acylase (PGA) derived from the strain Escherichia coli W ATCC 9637. Their size and copy number (CN) in E. coli W were determined (kb; CN): pRK1 (80; 3.4), pRK2 (5.1; 71), and pRK3 (4.8; 13.7). Strain E. coli RE2 harboring these plasmids was used for selection of strains with reduced number of plasmids: the strain RE3 without plasmid pRK1 and the plasmid-less strain cERE3 were isolated. Indigenous plasmids did not code for the resistance determinants against 23 antibiotics and 10 heavy metals.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Sobotková L,Grafková J,Stĕpánek V,Vacík T,Maresová H,Kyslík Pdoi
10.1007/BF02818544keywords:
subject
Has Abstractpub_date
1999-01-01 00:00:00pages
263-6issue
3eissn
0015-5632issn
1874-9356journal_volume
44pub_type
杂志文章abstract::Treatment of Helicobacter pylori cells with several chaotropic agents resulted in different degrees of inhibition in the binding of the bacteria to hemin and Congo-red dye. Polyanions also yielded a >50% inhibitory effect. Furthermore, hydrophobic interaction chromatography was used to determine the relative surface h...
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