Abstract：
:The ribosomal stalk is involved directly in the interaction of the elongation factors with the ribosome during protein synthesis. The stalk is formed by a complex of five proteins, four small acidic polypeptides and a larger protein which directly interacts with the rRNA at the GTPase center. In eukaryotes, the acidic components correspond to the 12 kDa P1 and P2 proteins, and the RNA binding component is protein P0. All these proteins are found to be phosphorylated in eukaryotic organisms. Previous in vitro data suggested this modification was involved in the activity of this structure. To confirm this possibility a mutational study has shown that phosphorylation takes place at a serine residue close to the carboxyl end of proteins P1, P2 and P0. This serine is part of a consensus casein kinase II phosphorylation site. However, by using a yeast strain carrying a temperature sensitive mutant, it has been shown that CKII is probably not the only enzyme responsible for this modification. Three new protein kinases, RAPI, RAPII and RAPIII, have been purified and compared with CKII and PK60, a previously reported enzyme that phosphorylates the stalk proteins. Differences among the five enzymes have been studied. It has also been found that some typical effectors of the PKC kinase stimulate the in vitro phosphorylation of the stalk proteins. All the data available suggest that phosphorylation, although it is not involved in the interaction of the acidic proteins with the ribosome, affects ribosome activity and might participate in some ribosome regulatory mechanism.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Rodriguez-Gabriel MA,Bou G,Briones E,Zambrano R,Remacha M,Ballesta JPdoi
10.1007/BF02816234keywords:
subject
Has Abstractpub_date
1999-01-01 00:00:00pages
153-63issue
2eissn
0015-5632issn
1874-9356journal_volume
44pub_type
杂志文章,评审abstract：:As a part of a program to develop starter cultures aiding in the spoilage control and sanitation of African fermented foods, a cereal-based food ('ogi' and its solid form 'agidi' or 'eko') was prepared using a bacteriocin-producing Lactobacillus strain as the starter culture. The survival of an enterotoxigenic Escheri...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02898642
更新日期：1997-01-01 00:00:00
abstract：:Lengths of permuted regions in the P22 and L phage genomes were estimated from the relative yields of DNA in many electrophoretic bands obtained using several restriction endonucleases. It was found that 3.6 kb (8.7%) of P22-DNA and 7.2 kb (17.8%) of L-DNA were circularly permuted. In both phages the sequential packag...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02933145
更新日期：1992-01-01 00:00:00
abstract：:Gut microbiome lives in the intestinal tract of animals and plays an important role in almost all life processes. Gut microbiome balance is beneficial to health, and imbalance leads to many diseases, one of which is obesity epidemic. However, gut microbiome is also influenced by host hormone, and different gut microbi...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-019-00704-4
更新日期：2019-11-01 00:00:00
abstract：:The rosettes formed by mouse peritoneal macrophages or DCH-5 cells and TNP-erythrocytes coated with anti-TNP antibodies of different isotypes were inhibited to various extent by monosaccharides. The most effective inhibitors were N-acetylglucosamine, glucosamine, mannose and N-acetylneuraminic acid in 1-5 mmol/L conce...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814584
更新日期：1992-01-01 00:00:00
abstract：:Euglena ignobilis cells in natural puddle water of pH 7.8, when kept at 21 +/- 2 degrees C and under continuous light (intensity of approximately 30 micromol m(-2) s(-1)) in a culture chamber, decreased their speed of movement from > or = 78000 microm/min (after a 12-h cultivation), to 850-1300 microm/min after 18 h. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931412
更新日期：2005-01-01 00:00:00
abstract：:The color of light (white, red, blue, and green) had a significant effect on the growth and reproductive processes (both in the nucleocytoplasmic and chloroplast compartment of the cells) in synchronous cultures of Scenedesmus obliquus. This effect decreased in the order red > white > blue > green. In the same order, ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931828
更新日期：2006-01-01 00:00:00
abstract：:This study was undertaken to develop a new Gram-staining machine controlled by a micro-controller and to investigate the quality of slides that were stained in the machine. The machine was designed and produced by the authors. It uses standard 220 V AC. Staining, washing, and drying periods are controlled by a timer b...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818557
更新日期：1999-01-01 00:00:00
abstract：:Inhibition of methanogenesis in ruminal cultures was attempted by hindering thiamine availability through its degradation by 'polyphenols' and competition for active sites on enzymes and transporters using thiamine structural analogs. Effects on fermentation were small and not consistently reversed by adding thiamine....
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-009-0075-9
更新日期：2009-11-01 00:00:00
abstract：:In this study, a method combining Raman spectroscopy with chemometric analysis was developed for detection of phage presence in raw milk and discrimination of Streptococcus thermophilus and Lactobacillus bulgaricus phages which are among the main phages causing problems in dairy industry. For this purpose, S. thermoph...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-018-0604-5
更新日期：2018-09-01 00:00:00
abstract：:Infiltration of immune cells into CNS is one of the essential events in multiple sclerosis (MS) development. Adhesion molecules like the intercellular adhesion molecule 1 (ICAM-1) play critical role in this process. Therefore, the ICAM1 gene containing two important single-nucleotide polymorphisms (SNPs) belongs to ca...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-017-0499-6
更新日期：2017-07-01 00:00:00
abstract：:Cryptococcus neoformans is encapsulated yeast that causes cryptococcosis. The cryptococcal meningitis may cause neuropsychiatric symptoms. Here, we evaluated the in vitro activity of amphotericin B (AMB), chlorpromazine (CLOR), and sertraline (SERT) alone or in combination against clinical isolates of C. neoformans co...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-016-0449-8
更新日期：2016-09-01 00:00:00
abstract：:Mice (strains Balb/c and A/J) received an intravenous infusion of bilirubin for a 1 d period. The infusion was delivered at various phases of the primary reaction; the degree of the immune response was expressed as the number of antibody-forming cells against sheep erythrocytes. Bilirubin infusion during both the indu...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02897214
更新日期：1980-01-01 00:00:00
abstract：:There is a growing interest in the use of bioinoculants to assist mineral fertilizers in improving crop production and yield. Azotobacter and Pseudomonas are two agriculturally relevant strains of bacteria which have been established as efficient bioinoculants. An experiment involving addition of graded concentrations...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-017-0495-x
更新日期：2017-05-01 00:00:00
abstract：:The addition of a nitrogen (nitrate) and carbon sources (acetate, citrate and fructose) and phosphate deficiency (nitrate medium deficient in phosphate) under unaerated conditions induced akinete differentiation in Anabaena torulosa. Aerated cultures of this organism in these nutrients did not differentiate akinetes. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02817617
更新日期：2000-01-01 00:00:00
abstract：:The pathogenesis of bacillary dysentery can be studied at different levels of integration of the cellular components that constitute the colonic mucosal barrier. We considered the interaction of Shigella flexneri in three experimental systems that provide complementary information and a scheme of events occurring in h...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02818608
更新日期：1998-01-01 00:00:00
abstract：:Thirty-three fully breast-fed infants aged between 1 and 12 weeks were screened for bifidobacteria in feces. Bifidobacteria counts in most fecal samples determined both by TPY agar and FISH procedure ranged from 10(8) to 10(11) CFU/g. Three infants did not contain any bifidobacteria in their fecal samples. One child w...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931404
更新日期：2004-01-01 00:00:00
abstract：:Almost all dried vegetative trichomes ofAnabaena iyengarii, Westiellopsis prolifica andNostochopsis lobatus died within 1 h, while those ofOscillatoria acuminata retained viability to some extent for 1 d under similar storage conditions. The viability of dried vegetative trichomes ofO. acuminata decreased about equall...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02903715
更新日期：1999-01-01 00:00:00
abstract：:The diversity and structure of bacterial and actinobacteral diversity and communities were determined in a metallophytic grassland soil from an upland site in northern England. The community profiles were subjected to multivariate analyses using correspondence and cluster analyses. The total bacterial community divers...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-009-0042-5
更新日期：2009-01-01 00:00:00
abstract：:The present paper reports a modified method for isolation of lysostaphin--a bacteriolytic agent with specific affinity for staphylococcal cell wall. The proposed purification scheme includes three steps. The first procedure is ultrafiltration through a membrane filter giving a yield of 75.6%. The result of ultrafiltra...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814386
更新日期：1993-01-01 00:00:00
abstract：:A novel immunosensing device based on a piezoelectric sensor for direct detection of the biological warfare agent Francisella tularensis was developed. This sensor includes mouse polyclonal antibody immobilized in a layer of protein A covalently linked to the gold electrode of the sensor. The immunosensor is able to d...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02932086
更新日期：2007-01-01 00:00:00
abstract：:Ten thermophilic Gram-positive bacteria were isolated from various soils of Saudi Arabia. The strains are spore-forming rods belonging to the species Bacillus stearothermophilus. The cells are motile, strictly aerobic, catalase and oxidase positive. The sporangia appear to be swollen and their position varies from ter...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02927328
更新日期：1981-01-01 00:00:00
abstract：:Thirty-four thiosemicarbazones and S-alkyl thiosemicarbazones, and some of their Zn(II) and Pd(II) complexes were obtained and purified to investigate antimicrobial activity. MIC values of the compounds were determined by the disc diffusion method against Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pse...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02932132
更新日期：2007-01-01 00:00:00
abstract：:Cyanobacteria are one of the most successful and oldest forms of life that are present on Earth. They are prokaryotic photoautotrophic microorganisms that colonize so diverse environments as soil, seawater, and freshwater, but also stones, plants, or extreme habitats such as snow and ice as well as hot springs. This d...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/s12223-018-0626-z
更新日期：2018-09-01 00:00:00
abstract：:The possibility of utilizing derivatives of 4-methylumbelliferone (4-MUBF) for the determination of activity of enzymes from the group of hydrolyses was tested in 20 strains of 12 species of mycobacteria. The method proved to be suitable especially for determination of arylsulphatase, beta-D-galactose and acid phospha...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876698
更新日期：1980-01-01 00:00:00
abstract：:Four in vitro experiments were set up to verify the colonization potential of ectomycorrhizal (EcM) Cenococcum geophilum FR. (strain CGE-4), saprotrophic Geomyces pannorum (LINK) SIGLER & CARMICHAEL (GPA-1) and a frequent root-associated, potentially ericoid mycorrhiza (ErM)-forming Meliniomyces variabilis Hambleton &...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02932096
更新日期：2007-01-01 00:00:00
abstract：:Substitution with NO2, Cl, Br and I into position 6 of 2-benzthiazolthiol derivatives increased their antimicrobial efficiency while the SH group is preserved. This increase affected both G+ and G--bacteria, mycobacteria and some fungi. The degree of efficiency of the benzthiazol derivatives is also influenced by the ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876583
更新日期：1978-01-01 00:00:00
abstract：:Antibacterial effect of chitooligosaccharides (COS) and low molar mass chitosans (LMWC) is considered as one of the most important characteristics of chitosan (CS) hydrolysates. Here, we show the in vitro effect of different COS, LMWC, and CS on representative anaerobic bacteria isolated from human colon as a possibil...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-012-0138-1
更新日期：2012-07-01 00:00:00
abstract：:The survival of Herbaspirillum spp. cells added directly or encapsulated in alginate beads and colonization of wheat roots was evaluated in soil microcosms. Cells entrapped in alginate in the presence of JNFb-broth and introduced into unplanted non-sterile clay loamy and sandy soils survived better than cells added di...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02825879
更新日期：2001-01-01 00:00:00
abstract：:The kinetics of extracellular neutral proteinase synthesis by an isogenic stringent (IS58) and a relaxed (IS56) strain of B. subtilis were compared. The specific enzyme formation rate by the stringent strain was higher than that of the relaxed one. Norvaline addition (1 mg/mL) induced the formation of pppGpp and ppGpp...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02883234
更新日期：1987-01-01 00:00:00
abstract：:Candidiases, infections caused by germination forms of the Candida fungus, represent a heterogeneous group of diseases from systemic infection, through mucocutaneous form, to vulvovaginal form. Although caused by one organism, each form is controlled by distinct host immune mechanisms. Phagocytosis by polymorphonuclea...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02931313
更新日期：2007-01-01 00:00:00
