当前位置： SCI文献检索 > FOLIA MICROBIOLOGICA期刊下所有文献 > Purification and biochemical characterization of a mycelial alkaline phosphatase without DNAase activity produced by Aspergillus caespitosus.

Purification and biochemical characterization of a mycelial alkaline phosphatase without DNAase activity produced by Aspergillus caespitosus.

Abstract：

:Biochemical properties of a termostable alkaline phosphatase obtained from the mycelium extract of A. caespitosus were described. The enzyme was purified 42-fold with 32% recovery by DEAE-cellulose and concanavalin A-Sepharose chromatography. The molar mass estimated by Sephacryl S-200 or by 7% SDS-PAGE was 138 kDa and 71 kDa, respectively, indicating a homodimer. Temperature and pH optima were 80 degrees C and pH 9.0. This enzyme was highly glycosylated (approximately 74% saccharide content). The activity was enhanced by Mg2+ (19-139%), NH4+ (64%), Na+ (51%) and Mn2+ (38%). 4-Nitrophenyl phosphate (4-NPP) was preferentially hydrolyzed, but glucose 1-phosphate (93%), UTP (67%) and O-phosphoamino acids also acted as substrates. V(lim) and K(m) were 3.78 nkat per mg protein and 270 micromol/L in the absence of Mg2+ and 7.35 nkat per mg protein and 410 micromol/L in the presence of Mg2+, using 4-NPP as substrate. The purified alkaline phosphatase removed the 5'-phosphate group of a linearized plasmid without showing DNAase activity, indicating its potential for recombinant DNA technology.

journal_name

Folia Microbiol (Praha)

journal_title

Folia microbiologica

authors

Guimarães LH,Júnior AB,Jorge JA,Terenzi HF,Polizeli ML

doi

10.1007/BF02931303

subject

Has Abstract

pub_date

2007-01-01 00:00:00

pages

231-6

issue

3

eissn

0015-5632

issn

1874-9356

journal_volume

52

pub_type

杂志文章

相关文献

FOLIA MICROBIOLOGICA文献大全
  • Short-term stability of Borrelia garinii in cerebrospinal fluid.

    abstract：:The aim of our study was to find out the optimal conditions for short-term storage of cerebrospinal fluid (CSF) samples for direct diagnosis of Lyme disease. A mixture of Borrelia-negative CSFs spiked with a defined amount of cultured Borrelia garinii was used. Borrelia stability was investigated over 7 days at four d...

    journal_title：Folia microbiologica

    pub_type： 杂志文章

    doi：10.1007/s12223-015-0408-9

    authors： Berenová D,Krsek D,Šípková L,Lukavská A,Malý M,Kurzová Z,Hořejší J,Kodym P

    更新日期：2016-01-01 00:00:00

  • Recombinant single-chain Fv antibodies that recognize the p25 protein of the Maedi-Visna virus.

    abstract：:Single chain Fv (scFv) antibodies (generated by phage display technology, molecules representing new and efficient tools in the research and diagnostics of infectious diseases) against the capsid protein (p25) of Maedi-Visna virus were selected. Several clones of p25 specific scFv antibodies were identified; one of th...

    journal_title：Folia microbiologica

    pub_type： 杂志文章

    doi：10.1007/BF02931380

    authors： Celer V,Blazek D,Navrátilová I,Skládal P,Blacklaws B,Bujdoso R

    更新日期：2003-01-01 00:00:00

  • Isolation and characterization of lactic acid bacteria from pobuzihi (fermented cummingcordia), a traditional fermented food in Taiwan.

    abstract：:Lactobacillus pobuzihii is a novel species which has been previously found in pobuzihi (fermented cummingcordia), a traditional fermented food in Taiwan. However, the lactic acid bacteria (LAB) microflora in pobuzihi has not been studied in detail. In this study, LAB from pobuzihi were isolated, identified, and charac...

    journal_title：Folia microbiologica

    pub_type： 杂志文章

    doi：10.1007/s12223-012-0188-4

    authors： Chen YS,Wu HC,Wang CM,Lin CC,Chen YT,Jhong YJ,Yanagida F

    更新日期：2013-03-01 00:00:00

  • Production of polyclonal antibodies to a recombinant coat protein of potato virus Y.

    abstract：:The gene encoding the coat protein (CP) of a potato virus Y (PVY) was cloned into expression vector pMPM-A4Omega. PVY CP was expressed in Escherichia coli and the purified recombinant protein was used for raising rabbit polyclonal antibodies. The sera and antibodies were tested for the detection of PVY in the laborato...

    journal_title：Folia microbiologica

    pub_type： 杂志文章

    doi：10.1007/s12223-008-0067-1

    authors： Folwarczna J,Plchová H,Moravec T,Hoffmeisterová H,Dedic P,Cerovská N

    更新日期：2008-01-01 00:00:00

  • Inhibition of lymphoproliferative response and its restoration with a glucan immunomodulator in mice with experimental larval toxocarosis.

    abstract：:A high infective dose of Taxocara canis eggs (2,500 eggs per mouse) induced a partial immunosuppression in mice, manifested by inhibition of the proliferative response of splenic T and B cells to polyclonal activators. A glucan immunomodulator given to infected animals at the beginning of the experiment showed a marke...

    journal_title：Folia microbiologica

    pub_type： 杂志文章

    doi：10.1007/BF02820794

    authors： Borosková Z,Reiterová K,Dubinský P,Tomasovicová O,Machnicka B

    更新日期：1998-01-01 00:00:00

  • Isolation and chemical characterization of compounds isolated from Aspergillus flavus conidia.

    abstract：:Three types of heterogeneous preparations and four types of preparations of polysaccharide nature were obtained in studies aimed at the isolation of active compounds from Aspergillus flavus conidia bearing their biological stimulatory activity. Extraction with trichloroacetic acid at 0 degrees C yielded a preparation ...

    journal_title：Folia microbiologica

    pub_type： 杂志文章

    doi：10.1007/BF02927425

    authors： Budínská O,Franĕk J,Kubín V,Fiedorov BA,Stasilevitch ZK

    更新日期：1981-01-01 00:00:00

  • Aspergillus niger pH 2.1 optimum acid phosphatase with high affinity for phytate.

    abstract：:An extracellular acid phosphatase isolated from the culture of a wild strain Aspergillus niger, producing the dephosphorylating 3-phytase, was obtained in a homogeneous form by sequential application of ultrafiltration through PS 50 membrane, gel filtration on Sephadex G-100 and ion exchange chromatography on DEAE-Sep...

    journal_title：Folia microbiologica

    pub_type： 杂志文章

    doi：10.1007/BF02931618

    authors： Gargova S,Sariyska M,Angelov A,Stoilova I

    更新日期：2006-01-01 00:00:00

  • Effect of volatile and gaseous metabolites of germinating pea seeds on Micromycetes.

    abstract：:Differences in the effect of volatile and gaseous metabolites of germinating pea seeds on the germination of spores of Mucor racemosus and macroconidia of Fusarium oxysporum are described. Germination of spores of M. racemosus was inhibited by seed metabolites whereas germination of macroconidia of F. oxysporum was st...

    journal_title：Folia microbiologica

    pub_type： 杂志文章

    doi：10.1007/BF02933019

    authors： Catská V

    更新日期：1980-01-01 00:00:00

  • 5-Deoxy-5-fluoro-L-sorbose originating from 2-deoxy-2-fluoro-D-glucitol by fermentation with Acetomonas oxydans.

    abstract：:Using fermentation with a selected strain of Acetomonas oxydans it was possible to convert 2-deoxy-2-fluro-D-glucitol to 5-deoxy-5-fluoro-L-sorbose, in agreement with Bertrand's and Hudson's rule. The last-named compound was isolated in a yield of 88%. Both compounds were little toxic against Acetomonas oxydans. ...

    journal_title：Folia microbiologica

    pub_type： 杂志文章

    doi：10.1007/BF02877659

    authors： Kulhánek M,Tadra M,Pacák J,Trejbalová H,Cerný M

    更新日期：1977-01-01 00:00:00

  • Establishment and follow-up of bifidobacterial species in the gut of healthy bottle-fed infants of 1-4 months age.

    abstract：:Twenty-one healthy bottle-fed infants were screened monthly (1-4 months) for bifidobacteria in their stools. Bifidobacteria were detected by culture and isolates specified by PCR. Alternatively, direct PCR in undiluted fecal suspensions was carried out for detection of bifidobacteria under the cultural detection level...

    journal_title：Folia microbiologica

    pub_type： 杂志文章

    doi：10.1007/BF02931594

    authors： Mullié C,Romond MB,Izard D

    更新日期：2006-01-01 00:00:00

  • Repression of the synthesis of exocellular and intracellular proteinases in Bacillus megaterium.

    abstract：:The synthesis of exocellular proteinase decreases with increasing concentration of amino acids in the growth medium. After removal of amino acids the enzyme synthesis is gradually restored to normal values. The presence of inhibitors of transcription (actinomycin D) or translation (chloramphenicol) blocks the restorat...

    journal_title：Folia microbiologica

    pub_type： 杂志文章

    doi：10.1007/BF02875957

    authors： Moravcová J,Chaloupka J

    更新日期：1984-01-01 00:00:00

  • Eradication of Enterococcus faecalis by phage therapy in chronic bacterial prostatitis--case report.

    abstract：:The treatment of three patients suffering from chronic bacterial prostatitis who were qualified for an experimental phage therapy protocol managed at the Phage Therapy Unit in Wrocław is described. They had previously been treated unsuccessfully with long-term targeted antibiotics, autovaccines, and laser biostimulati...

    journal_title：Folia microbiologica

    pub_type： 杂志文章

    doi：10.1007/s12223-009-0064-z

    authors： Letkiewicz S,Miedzybrodzki R,Fortuna W,Weber-Dabrowska B,Górski A

    更新日期：2009-09-01 00:00:00

  • Superantigen types in Staphylococcus aureus isolated from patients with cystic fibrosis.

    abstract：:The screening of 17 SAg genes of S. aureus isolated from the sputum of cystic fibrosis (CF) patients revealed that among 47 genetically different strains, 39 (83 %) carried SAg genes. Superantigens forming enterotoxin gene cluster were detected in 20 strains. The 2nd most common superantigen type was selk detected in ...

    journal_title：Folia microbiologica

    pub_type： 杂志文章

    doi：10.1007/BF02931628

    authors： Bania J,Dabrowska A,Rózalska B,Sadowska B,Wieckowska-Szakiel M,Korzekwa K,Zarczyńska A,Bystroń J,Chrzanowska J,Molenda J

    更新日期：2006-01-01 00:00:00

  • Gamma herpesviruses: pathogenesis of infection and cell signaling.

    abstract：:Altered cell signaling is the molecular basis for cell proliferation occurring in association with several gamma herpesvirus infections. Three gamma herpesviruses, namely EBV/HHV-4, KSHV/HHV-8 and the MHV-68 (and/or MHV-72) and their unusual cell-pirated gene products are discussed in this respect. The EBV, KSHV as we...

    journal_title：Folia microbiologica

    pub_type： 杂志文章,评审

    doi：10.1007/BF02931360

    authors： Rajcáni J,Kúdelová M

    更新日期：2003-01-01 00:00:00

  • Effects of increasing doses of UV-B radiation on photosynthesis and motility in Chlamydomonas reinhardtii.

    abstract：:Effects of increasing doses of UV-B radiation on photosynthetic efficiency (PE) and motility in Chlamydomonas reinhardtii were investigated. The exposure time ranged from 10 to 120 min at 0.73 W/m2 UV-B radiation. A decrease in compensation points (CP) was followed by an increase in PE on extending the UV-B treatment ...

    journal_title：Folia microbiologica

    pub_type： 杂志文章

    doi：10.1007/BF02817448

    authors： Danilov RA,Ekelund NG

    更新日期：2000-01-01 00:00:00

  • Transcriptional regulators of seven yeast species: comparative genome analysis. Review.

    abstract：:The regulation of gene transcription allows yeast cells to respond properly to changing environmental conditions. Several protein complexes take part in this process. They involve RNA polymerase complexes, chromatin remodeling complexes, mediators, general transcription factors and specific transcriptional regulators....

    journal_title：Folia microbiologica

    pub_type： 杂志文章,评审

    doi：10.1007/s12223-008-0044-8

    authors： Drobná E,Bialková A,Subík J

    更新日期：2008-01-01 00:00:00

  • Fatty acid analysis of Stenotrophomonas maltophilia clinical strains showing different susceptibility to antibiotics at 30 and 37 degrees C.

    abstract：:Isolates of Stenotrophomonas maltophilia species display the feature "temperature-dependent susceptibility" (TDS) to antibiotics. Both 30TDS strains (at least 4 times lower value of minimum inhibitory concentration (MIC) of an antibiotic at 30 than at 37 degrees C) and 37TDS strains (at least 4 times lower value of MI...

    journal_title：Folia microbiologica

    pub_type： 杂志文章

    doi：10.1007/BF02818682

    authors： Hejnar P,Chmela Z,Rypka M

    更新日期：2002-01-01 00:00:00

  • Distribution of pathogenicity island markers in commensal and uropathogenic Escherichia coli isolates.

    abstract：:Uropathogenic Escherichia coli (UPEC) isolates contain large genomic segments, termed pathogenicity islands (PAIs), that contribute to their virulence. A total of 150 UPEC and 50 commensal E. coli isolates from outpatients were investigated for antimicrobial susceptibility and the presence of eight PAI markers. One hu...

    journal_title：Folia microbiologica

    pub_type： 杂志文章

    doi：10.1007/s12223-015-0433-8

    authors： Samei A,Haghi F,Zeighami H

    更新日期：2016-05-01 00:00:00

  • Potential of the strain Raoultella sp. KDF8 for removal of analgesics.

    abstract：:The bacterial strain KDF8 capable of growth in the presence of diclofenac and codeine analgesics was obtained after chemical mutagenesis of nature isolates from polluted soils. The strain KDF8 was identified as Raoultella sp. based on its morphology, biochemical properties, and 16S rRNA gene sequence. It was deposited...

    journal_title：Folia microbiologica

    pub_type： 杂志文章

    doi：10.1007/s12223-017-0563-2

    authors： Palyzová A,Zahradník J,Marešová H,Sokolová L,Kyslíková E,Grulich M,Štěpánek V,Řezanka T,Kyslík P

    更新日期：2018-05-01 00:00:00

  • Phenotypic and genotypic identification of lactic acid bacteria isolated from traditional pickles of the Çubuk region in Turkey.

    abstract：:A total of 152 lactic acid bacteria (LAB) were isolated from pickles produced in the Ankara-Çubuk region. These isolates were clustered into eight groups on the basis of their phenotypic characteristics including cell morphology, CO2 production from glucose, growth at 10 and 45 °C, growth in 6.5 % NaCl, and growth at ...

    journal_title：Folia microbiologica

    pub_type： 杂志文章

    doi：10.1007/s12223-014-0363-x

    authors： Bağder Elmacı S,Tokatlı M,Dursun D,Özçelik F,Şanlıbaba P

    更新日期：2015-05-01 00:00:00

  • Antitrichomonadal and other biological effects of sugar hydrazones and phenylosazones.

    abstract：:When investigating the possible antitrichomodal effect of 41 phenylsazones, nitro- and dinitro-phenylhydrazones a significant inhibitory effect on multiplication of Trichomonas foetus could be detected in 14 derivatives; eight derivatives were ineffective, other derivatives exhibited only a slight effect. The inhibito...

    journal_title：Folia microbiologica

    pub_type： 杂志文章

    doi：10.1007/BF02883838

    authors： Fuska J,Fusková A,Kettner M,Frank V,Linek K

    更新日期：1982-01-01 00:00:00

  • Alternative sources of biologically active substances.

    abstract：:The majority of antibiotics and substances with diverse biological activity used in medicine are produced by actinomycetes, nonfilamentous bacteria and fungi. Other microorganisms, such as myxobacteria, pseudomonads, nocardias, basidiomycetes, marine microorganisms, enterobacteria, halobacteria, hyperthermophiles etc....

    journal_title：Folia microbiologica

    pub_type： 杂志文章,评审

    doi：10.1007/BF02993460

    authors： Bĕhal V

    更新日期：2003-01-01 00:00:00

  • Characterization of clinical isolates of group C and G streptococci on the basis of protein G gene.

    abstract：:Treatment of human group C and G streptococci with cyanogen bromide results in solubilization of surface protein G molecules. Strain-to-strain variation in the quantity, molar mass and functional activity of protein G extracted from representative group C and G isolates led to the identification of three structurally ...

    journal_title：Folia microbiologica

    pub_type： 杂志文章

    doi：10.1007/BF02825666

    authors： Gupalova T,Voltchek N,Totolyan A

    更新日期：1999-01-01 00:00:00

  • The antimicrobial action of chitosan, low molar mass chitosan, and chitooligosaccharides on human colonic bacteria.

    abstract：:Antibacterial effect of chitooligosaccharides (COS) and low molar mass chitosans (LMWC) is considered as one of the most important characteristics of chitosan (CS) hydrolysates. Here, we show the in vitro effect of different COS, LMWC, and CS on representative anaerobic bacteria isolated from human colon as a possibil...

    journal_title：Folia microbiologica

    pub_type： 杂志文章

    doi：10.1007/s12223-012-0138-1

    authors： Simůnek J,Brandysová V,Koppová I,Simůnek J Jr

    更新日期：2012-07-01 00:00:00

  • Arsenic-resistant Pseudomonas spp. and Bacillus sp. bacterial strains reducing As(V) to As(III), isolated from Alps soils, Italy.

    abstract：:Five arsenic-resistant bacterial strains (designated MP1400, MP1400a, MP1400d, APSLA3, and BPSLA3) were isolated from soils collected at the Alps region (Italy), which showed no contamination by arsenic. Phylogenetic analysis of the 16S rRNA gene sequences assigned them to the genera Pseudomonas and Bacillus. Bacillus...

    journal_title：Folia microbiologica

    pub_type： 杂志文章

    doi：10.1007/s12223-011-0010-8

    authors： Pepi M,Protano G,Ruta M,Nicolardi V,Bernardini E,Focardi SE,Gaggi C

    更新日期：2011-01-01 00:00:00

  • Subtyping of Listeria monocytogenes isolates by actA gene sequencing, PCR-fingerprinting, and cell-invasion assay.

    abstract：:Analysis of actA gene sequence polymorphism has been shown to be an effective and relatively inexpensive method for subtyping Listeria monocytogenes isolates, allowing the division of the population of this species into two deeply separate lineages. This sequence-based method as well as PCR-mediated fingerprinting wer...

    journal_title：Folia microbiologica

    pub_type： 杂志文章

    doi：10.1007/s12223-009-0003-z

    authors： Bania J,Zarczyńska A,Molenda J,Dabrowska A,Kosek-Paszkowska K,Wieckowska-Szakiel M,Rózalska B

    更新日期：2009-01-01 00:00:00

  • Enzyme synthesis of L-tryptophan.

    abstract：:Enzyme synthesis of tryptophan from indole, pyruvate and ammonium salts was studied using Escherichia coli cells exhibiting a significant tryptophanase activity. In addition to the effect of cultivation medium composition and cultivation conditions, factors affecting the course of the conversion were investigated. Pro...

    journal_title：Folia microbiologica

    pub_type： 杂志文章

    doi：10.1007/BF02820485

    authors： Zeman R,Plachý J,Bulantová H,Sikyta B,Pavlasová E,Stejskalová E

    更新日期：1990-01-01 00:00:00

  • Survival of and wheat-root colonization by alginate encapsulated Herbaspirillum spp.

    abstract：:The survival of Herbaspirillum spp. cells added directly or encapsulated in alginate beads and colonization of wheat roots was evaluated in soil microcosms. Cells entrapped in alginate in the presence of JNFb-broth and introduced into unplanted non-sterile clay loamy and sandy soils survived better than cells added di...

    journal_title：Folia microbiologica

    pub_type： 杂志文章

    doi：10.1007/BF02825879

    authors： el-Komy HM

    更新日期：2001-01-01 00:00:00

  • Pullulanase: model protein substrate for the general secretory pathway of gram-negative bacteria.

    abstract：:Pullulanase of Klebsiella oxytoca is one of a wide variety of extracellular proteins that are secreted by Gram-negative bacteria by the complex main terminal branch (MTB) of the general secretory pathway. The roles of some of the 14 components of the MTB are now becoming clear. In this review it is proposed that most ...

    journal_title：Folia microbiologica

    pub_type： 杂志文章,评审

    doi：10.1007/BF02818976

    authors： Pugsley AP,Francetic O,Hardie K,Possot OM,Sauvonnet N,Seydel A

    更新日期：1997-01-01 00:00:00

  • Biotransformation of 1,8-cineole by solid-state fermentation of Eucalyptus waste from the essential oil industry using Pleurotus ostreatus and Favolus tenuiculus.

    abstract：:Biotechnological conversion of low-cost agro-industrial by-products, such as industrial waste or terpenes from the distillation of essential oils from plants into more valuable oxygenated derivatives, can be achieved by using microbial cells or enzymes. In Argentina, the essential oil industry produces several tons of...

    journal_title：Folia microbiologica

    pub_type： 杂志文章

    doi：10.1007/s12223-015-0422-y

    authors： Omarini A,Dambolena JS,Lucini E,Jaramillo Mejía S,Albertó E,Zygadlo JA

    更新日期：2016-03-01 00:00:00