Abstract：
:The gene encoding the coat protein (CP) of a potato virus Y (PVY) was cloned into expression vector pMPM-A4Omega. PVY CP was expressed in Escherichia coli and the purified recombinant protein was used for raising rabbit polyclonal antibodies. The sera and antibodies were tested for the detection of PVY in the laboratory host Nicotiana tabacum cv. Petit Havana SR1 and in various cultivars of the natural host Solanum tuberosum by ELISA as well as by Western blots. The antibodies can be used for the detection of the whole strain spectrum of PVY by indirect plate trapped antigen ELISA and Western blot, but not by double antigen sandwich ELISA.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Folwarczna J,Plchová H,Moravec T,Hoffmeisterová H,Dedic P,Cerovská Ndoi
10.1007/s12223-008-0067-1subject
Has Abstractpub_date
2008-01-01 00:00:00pages
438-42issue
5eissn
0015-5632issn
1874-9356journal_volume
53pub_type
杂志文章abstract：:Manganese peroxidase (MnP) was produced by shallow stationary cultures of Phanerochaete chrysosporium growing on N-limited medium. Decolorization of sulfonphthalein (SP) dyes by MnP was investigated. The MnP activity profile and decolorization of SP dyes was correlated and almost all dyes were decolorized at pH 4.0. T...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931512
更新日期：2003-01-01 00:00:00
abstract：:A group of sixteen esculin-positive fluorescent pseudomonads isolated from an underground brook flowing through a cave complex was characterized by biotyping, multiple enzyme restriction fragment length polymorphism analysis of 16S rDNA (MERFLP), ribotyping and whole-cell fatty-acid methyl-esters analysis (FAME). All ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931556
更新日期：2004-01-01 00:00:00
abstract：:The rosettes formed by mouse peritoneal macrophages or DCH-5 cells and TNP-erythrocytes coated with anti-TNP antibodies of different isotypes were inhibited to various extent by monosaccharides. The most effective inhibitors were N-acetylglucosamine, glucosamine, mannose and N-acetylneuraminic acid in 1-5 mmol/L conce...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814584
更新日期：1992-01-01 00:00:00
abstract：:Vinasse, a recalcitrant waste of the ethanol industry was employed for the production of polyhydroxyalkanoate (PHA) by the extremely halophilic archaeon, Haloarcula marismortui in shake flasks. The PHA was recovered by osmotic lysis of the cells and subsequent purification by sodium hypochlorite and organic solvents. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0092-3
更新日期：2012-01-01 00:00:00
abstract：:This study presents the effects of the probiotic and enterocin M-producing strain Enterococcus faecium AL41 on microbiota, phagocytic activity (PA), oxidative stress, performance and biochemical parameters when applied individually or in combination with Eleutherococcus senticosus in rabbits. The novelty of the study ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-015-0423-x
更新日期：2016-03-01 00:00:00
abstract：:Aeromonads represent bacteria thought to be primarily mostly autochthonous to aquatic environments. This study was focused on the relation with antibiotics and enterocins of identified Aeromonas species isolated from the intestine of trouts living in Slovakian aquatic sources. Intestinal samples from 50 trouts (3 Salm...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-018-0616-1
更新日期：2018-11-01 00:00:00
abstract：:The activities of inorganic pyrophosphatase, thymidine kinase and thymidine phosphorylase were measured in Ter-mutants of E. coli K12 which have a higher or a lower dTTP pool than the parent strain. The levels of inorganic pyrophosphatase and thymidine kinase were changed in the same direction and that of thymidine ph...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02884076
更新日期：1983-01-01 00:00:00
abstract：:This work was aimed to develop a multiplex PCR assay to detect infectious agents such as Clavibacter michiganensis subsp. michiganensis, Fusarium sp, Leveillula taurica, and begomoviruses in tomato (Solanum lycopersicum) plants. Specific primer sets of each pathogen were designed based on intergenic ribosomal RNA sequ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-012-0206-6
更新日期：2013-07-01 00:00:00
abstract：:Streptomyces caelestis DSM 40084 produces two osmolytes, viz. 2-O-(alpha-D-glucopyranosyl)-zeta-glyceric acid (GG) and trehalose. Both compounds were isolated and identified by nuclear magnetic resonance spectroscopy and mass spectrometry. A very sensitive regulation of the cell osmolytes was demonstrated in exponenti...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02932103
更新日期：2007-01-01 00:00:00
abstract：:A novel immunosensing device based on a piezoelectric sensor for direct detection of the biological warfare agent Francisella tularensis was developed. This sensor includes mouse polyclonal antibody immobilized in a layer of protein A covalently linked to the gold electrode of the sensor. The immunosensor is able to d...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02932086
更新日期：2007-01-01 00:00:00
abstract：:The antimicrobial and morphogenetic effects of fourteen newly synthesized 2-substituted derivatives of quinoline-4-carboxylic acid and quinoline-4-carboxamide were studied using G+ and G- bacteria, yeasts and filamentous fungi. The highest antimicrobial effects were found with substituted quinoline-4-carboxylic acid d...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02817551
更新日期：2000-01-01 00:00:00
abstract：:Hemolytic factor production by pathogenic Candida species is considered an important attribute in promoting survival within the mammal host through the ability to assimilate iron from the hemoglobin-heme group. Hemolytic capability has been evaluated for Candida species based on hemolysis zones on plate assay, analysi...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/s12223-018-0584-5
更新日期：2018-07-01 00:00:00
abstract：:Levels of phenotypic and genotypic diversity among seven Megasphaera elsdenii strains recovered from rumen contents of cattle, sheep and lambs were determined by a combination of antibiotic-resistance analysis and PCR fingerprint techniques targeted both to the ribosomal RNA operon (ARDRA, RISA) and the whole genome (...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931818
更新日期：2006-01-01 00:00:00
abstract：:We report the cloning and sequence analysis of a gamma-butyrolactone autoregulator regulatory island that includes an sscR gene encoding the gamma-butyrolactone autoregulator receptor from Streptomyces scabies NBRC 12914, a plant pathogenic strain. gamma-Butyrolactone autoregulators trigger secondary metabolism, and s...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-008-0017-y
更新日期：2008-01-01 00:00:00
abstract：:One-week dextran sulfate treatment of conventional (CV) immunodeficient (SCID) mice gave rise to acute colitis in the colon mucosa; germ-free (GF) SCID mice did not exhibit any changes in colon morphology. Dextran sulfate application to CV immunocompetent (BALB/c) mice did induce substantial changes in the colon mucos...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818004
更新日期：2001-01-01 00:00:00
abstract：:Three indigenous plasmids designated pRK1, pRK2 and pRK3 were identified among producers of penicillin G acylase (PGA) derived from the strain Escherichia coli W ATCC 9637. Their size and copy number (CN) in E. coli W were determined (kb; CN): pRK1 (80; 3.4), pRK2 (5.1; 71), and pRK3 (4.8; 13.7). Strain E. coli RE2 ha...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818544
更新日期：1999-01-01 00:00:00
abstract：:Glycerol-based medium (BM) with and without the addition of 1 g/L ascorbic acid (Asc) and/or 100 mg/L (+/-)-catechin (Cat) was tested for the storage of three commercial wine yeasts at -20 degrees C. The medium supplemented with Asc was also used to store 706 strains to verify the maintenance of the liquid state. A de...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-009-0036-3
更新日期：2009-01-01 00:00:00
abstract：:Escherichia coli strain HB101 harboring an expression plasmid bearing calf prochymosin gene under the control of the tac promoter was grown in the presence of IPTG with or without novobiocin at 28 and 40 degrees C, respectively. The differential rates of synthesis of prochymosin inclusions, and, for comparison, of bet...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02815545
更新日期：1998-01-01 00:00:00
abstract：:Antibacterial effect of chitooligosaccharides (COS) and low molar mass chitosans (LMWC) is considered as one of the most important characteristics of chitosan (CS) hydrolysates. Here, we show the in vitro effect of different COS, LMWC, and CS on representative anaerobic bacteria isolated from human colon as a possibil...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-012-0138-1
更新日期：2012-07-01 00:00:00
abstract：:Hydroxy, n-saturated, branched, dioic, and unsaturated fatty acids in six freshwater wild cyanobacteria (Chroococcus minutus, Lyngbya ceylanica, Merismopedia glauca, Nodularia sphaerocarpa, Nostoc linckia, and Synechococcus aeruginosus) collected from different lakes and springs of Israel have been identified by gas c...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931279
更新日期：2003-01-01 00:00:00
abstract：:Contemporary aspects of cerebrospinal fluid analysis are discussed, including the relationship to neuro-infective, autoimmune and other neurological diseases. The actual state of cerebrospinal fluid microbiological and cytological investigation and analysis of cerebrospinal fluid protein fractions are described in det...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02931522
更新日期：2003-01-01 00:00:00
abstract：:Proteinase production by Lactobacillus murinus was influenced by temperature, glucose concentration, initial pH and nitrogen sources. Maximum proteinase production occurred at 45 degrees C, pH 6.6 and with 0.5% (W/V) glucose. Tryptone, peptone and gelatin inhibited it. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02877263
更新日期：1987-01-01 00:00:00
abstract：:The uvrE (E. coli KS 114) cells carry a mutation in the gene that codes for helicase II. This is the protein responsible for replicative unwinding of double-helical DNA. The repair mode of such cells may be altered as compared with the wild type. The survival of uvrE cells during postirradiation incubation under inhib...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02922491
更新日期：1985-01-01 00:00:00
abstract：:Effects of increasing doses of UV-B radiation on photosynthetic efficiency (PE) and motility in Chlamydomonas reinhardtii were investigated. The exposure time ranged from 10 to 120 min at 0.73 W/m2 UV-B radiation. A decrease in compensation points (CP) was followed by an increase in PE on extending the UV-B treatment ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02817448
更新日期：2000-01-01 00:00:00
abstract：:The survival of Herbaspirillum spp. cells added directly or encapsulated in alginate beads and colonization of wheat roots was evaluated in soil microcosms. Cells entrapped in alginate in the presence of JNFb-broth and introduced into unplanted non-sterile clay loamy and sandy soils survived better than cells added di...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02825879
更新日期：2001-01-01 00:00:00
abstract：:For the first time, it was reported in France a cluster of autochthonous severe community-acquired (CA) infections due to the USA300 methicillin-resistant Staphylococcus aureus (MRSA) clone. The three cases belonged to the same family without any identified clue of abroad importation pathway. The domestic spread of US...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-014-0322-6
更新日期：2014-11-01 00:00:00
abstract：:The bacterial strain KDF8 capable of growth in the presence of diclofenac and codeine analgesics was obtained after chemical mutagenesis of nature isolates from polluted soils. The strain KDF8 was identified as Raoultella sp. based on its morphology, biochemical properties, and 16S rRNA gene sequence. It was deposited...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-017-0563-2
更新日期：2018-05-01 00:00:00
abstract：:The characterization of 11- and 18-residue peptaibols (peptides synthesized by peptide synthetases) at Trichoderma harzianum CECT 2413 (a filamentous fungus) was performed. Using a heterologous probe from tex1, the only peptaibol synthetase cloned and characterized so far in Trichoderma species, was cloned; a region t...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02932165
更新日期：2006-01-01 00:00:00
abstract：:A method is presented for the successful decontamination of sparingly soluble pteridine derivatives by microwave irradiation. The method is nondestructive, rapid and effective in eliminating contamination. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814514
更新日期：1991-01-01 00:00:00
abstract：:Anaerobic ammonium-oxidizing (anammox) bacteria play an essential part in nitrogen removal in constructed wetlands. The objective of the present study was to explore the spatiotemporal dynamics of anammox bacterial communities and the associated factors in a full-scale constructed wetland for the treatment of polluted...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-019-00726-y
更新日期：2020-04-01 00:00:00
