Abstract:
:The rosettes formed by mouse peritoneal macrophages or DCH-5 cells and TNP-erythrocytes coated with anti-TNP antibodies of different isotypes were inhibited to various extent by monosaccharides. The most effective inhibitors were N-acetylglucosamine, glucosamine, mannose and N-acetylneuraminic acid in 1-5 mmol/L concentrations. Even more efficient were glycopeptides isolated from IgG molecules. The Fc receptors (FcRs) released from DCH-5 cells during cultivation and gradually separated by affinity chromatography on immobilized IgG reacted with aggregated IgG and inhibited the rosette formation. The FcRs eluted by monosaccharides influenced mainly the number of rosettes mediated by IgA and IgE while those eluted with a glycine-HCl buffer inhibited preferentially IgG rosettes. As shown by SDS-PAGE the heterogeneity of the fraction eluted with a mixture of monosaccharides revealed one main component with an effective molar mass of 50 kg/mol. The glycine-HCl eluate contained two major components of 55 and 38 kg/mol. The IgG-Sepharose 4B bound all the fractions but only the binding of the 50 kg/mol molecule could be inhibited by monosaccharides.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Tucková L,Rejnek J,Zikán J,Vĕtvicka Vdoi
10.1007/BF02814584keywords:
subject
Has Abstractpub_date
1992-01-01 00:00:00pages
71-8issue
1eissn
0015-5632issn
1874-9356journal_volume
37pub_type
杂志文章abstract::Manganese peroxidase (MnP) was produced by shallow stationary cultures of Phanerochaete chrysosporium growing on N-limited medium. Decolorization of sulfonphthalein (SP) dyes by MnP was investigated. The MnP activity profile and decolorization of SP dyes was correlated and almost all dyes were decolorized at pH 4.0. T...
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journal_title:Folia microbiologica
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abstract::We constructed a SmaI genomic library of Cellulomonas biazotea DNA in E. coli and in the S. cerevisiae shuttle vector, YEP 24. Three clone were identified that conferred the ability for E. coli or S. cerevisiae transformants to produce carboxymethylcellulase (CMCase). Cells transformed with these clones were compared ...
journal_title:Folia microbiologica
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更新日期:1994-01-01 00:00:00
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更新日期:2014-11-01 00:00:00
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doi:10.1007/BF02932150
更新日期:2007-01-01 00:00:00
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更新日期:2004-01-01 00:00:00
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journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02927298
更新日期:1979-01-01 00:00:00
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journal_title:Folia microbiologica
pub_type: 杂志文章
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更新日期:1988-01-01 00:00:00
abstract::Eleven purified lectins were compared in their IgE-precipitating, mast-cell-degranulating and histamine-releasing activity and in eliciting skin allergic reactions in atopic or normal people. A significantly positive relationship could be proved between their IgE-precipitating activity and degranulation of IgE-sensiti...
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