Application of PCR, rep-PCR and RAPD techniques for typing of Lactococcus lactis strains.

Abstract:

:A collection of 34 lactococcal strains were characterized using the polymerase chain reaction (PCR) for the acmA gene, and for the 16S rDNA gene, and DNA fingerprinting methods for randomly amplified polymorphic DNA (RAPD) and repetitive extragenic palindrome-PCR (rep-PCR). PCR experiments corroborated the genotypic identification of Lactococcus lactis strains by RAPD; rep-PCR did not distinguish between L. lactis subspecies. In some cases, phenotypic classification of L. lactis subspecies did not correlate with genotypic characterization.

journal_title

Folia microbiologica

authors

Prodelalová J,Spanová A,Rittich B

doi

10.1007/BF02931464

keywords:

subject

Has Abstract

pub_date

2005-01-01 00:00:00

pages

150-4

issue

2

eissn

0015-5632

issn

1874-9356

journal_volume

50

pub_type

杂志文章
  • 2,2'-dimethoxy-4a,4a'-dehydrorugulosin (rugulin), a minor metabolite from Penicillium rugulosum.

    abstract::A new metabolite was isolated from the culture of Penicillium rugulosum and its structure was determined from physico-chemical data. Accompanying metabolites skyrin and rugulosin were characterized by UV, IR, CD, mass and NMR spectra. ...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02876598

    authors: Sedmera P,Podojil M,Vokoun J,Betina V,Nemec P

    更新日期:1978-01-01 00:00:00

  • Biological function of DNA methylation.

    abstract::Structural and functional properties of prokaryotic DNA methyltransferases are summarized. The different aspects of the role of DNA methylation which influences DNA-protein interaction in restriction and modification of DNA and in mismatch repair, DNA replication and gene expression are discussed. ...

    journal_title:Folia microbiologica

    pub_type: 杂志文章,评审

    doi:10.1007/BF02815658

    authors: Hubácek J

    更新日期:1992-01-01 00:00:00

  • Regulation of the activity of intracellular alanylaminopeptidase synthesized by Pseudomonas sp.

    abstract::Activity of purified alanylaminopeptidase of Pseudomonas sp. measured in the presence of the alanine derivative of 2-naphthoic acid (NA-Ala) is inhibited by 1,10-phenanthroline, EDTA, bestatin and amastatin; this finding supports the conclusion that this enzyme is a metallo-aminopeptidase. A decrease of its activity i...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02817643

    authors: Jankiewicz U,Bielawski W

    更新日期:2002-01-01 00:00:00

  • Short-term stability of Borrelia garinii in cerebrospinal fluid.

    abstract::The aim of our study was to find out the optimal conditions for short-term storage of cerebrospinal fluid (CSF) samples for direct diagnosis of Lyme disease. A mixture of Borrelia-negative CSFs spiked with a defined amount of cultured Borrelia garinii was used. Borrelia stability was investigated over 7 days at four d...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-015-0408-9

    authors: Berenová D,Krsek D,Šípková L,Lukavská A,Malý M,Kurzová Z,Hořejší J,Kodym P

    更新日期:2016-01-01 00:00:00

  • Plectin-like proteins are present in cells of Chlamydomonas eugametos (Volvocales).

    abstract::Using both monoclonal and polyclonal antibodies against mammalian plectin (multifunctional protein cross-linking cytoskeletal structures, mainly intermediate filaments, in mammalian cells), several putative isoforms of plectin-like proteins were found in protein extracts from the green alga Chlamydomonas eugametos (Vo...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02818794

    authors: Hendrychová J,Vítová M,Bisová K,Wiche G,Zachleder V

    更新日期:2002-01-01 00:00:00

  • Formation of extracellular neutral proteinase and the stringent response in Bacillus subtilis.

    abstract::The kinetics of extracellular neutral proteinase synthesis by an isogenic stringent (IS58) and a relaxed (IS56) strain of B. subtilis were compared. The specific enzyme formation rate by the stringent strain was higher than that of the relaxed one. Norvaline addition (1 mg/mL) induced the formation of pppGpp and ppGpp...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02883234

    authors: Riedel K,Schroeter A,Liebs P,Graba JP,Hecker M,Schrapel D

    更新日期:1987-01-01 00:00:00

  • Assessment of a bacteriocin-producing Lactobacillus strain in the control of spoilage of a cereal-based African fermented food.

    abstract::As a part of a program to develop starter cultures aiding in the spoilage control and sanitation of African fermented foods, a cereal-based food ('ogi' and its solid form 'agidi' or 'eko') was prepared using a bacteriocin-producing Lactobacillus strain as the starter culture. The survival of an enterotoxigenic Escheri...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02898642

    authors: Olasupo NA,Olukoya DK,Odunfa SA

    更新日期:1997-01-01 00:00:00

  • Molecular diversity of "Candidatus Phytoplasma mali" strains associated with apple proliferation disease in Bulgarian germplasm collection.

    abstract::A quarantine organism, "Candidatus Phytoplasma mali," is the causal agent of apple proliferation, one of the most important apple diseases in Europe. The genetic diversity of this pathogen in Central and Southern Europe has already been reported; however, almost no data exists from Eastern Europe. In this study, "Ca. ...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-018-0660-x

    authors: Fránová J,Koloniuk I,Lenz O,Sakalieva D

    更新日期:2019-05-01 00:00:00

  • Aliphatic 1,2-alkanolamines--inhibitors of beta-glucanase from Candida utilis.

    abstract::Aliphatic 1,2-alkanolamines inhibited beta-glucanase from Candida utilis. The highest inhibitory effect was observed with 1-dodecylamino-3-chloro-2-propanol (Ki = 1.0 mumol/L) and it was much higher than that of D-glucono-delta-lactone. Simple 1,2-alkanolamines, like 2-aminoethanol or 2-methylaminoethanol, did not exh...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02898763

    authors: Steiner B,Omelková J,Proksa B,Sasinková V,Koós M

    更新日期:1993-01-01 00:00:00

  • Keratinophilic and other fungi isolated from combine harvester wheat and sorghum dusts and from the atmosphere of winnow sites in Egypt.

    abstract::Three methods were used for the isolation of fungi in the present investigation: the dilution-plate method, hair-baiting technique with horse hairs and the "exposed plate" method. Sabouraud's glucose agar at 28 degrees C was also used as isolation medium. 102 species and 2 species varieties belonging to 36 genera were...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02821282

    authors: Moubasher AH,Abdel-Hafez SI,Shoreit AA,Ismail MA

    更新日期:1990-01-01 00:00:00

  • Local distribution of ectomycorrhizae-associated basidiomycetes in forest soil correlates with the degree of soil organic matter humification and available electrolytes.

    abstract::Spatial distribution of ectomycorrhizae-associated basidiomycetes was determined in oakbirch forest using terminal restriction fragment length polymorphism (T-RFLP) analysis. The data were correlated with actual soil humidity, pH, electric conductivity of the soil extract, absorbance A(465) and A(665) of water and alk...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-010-0076-8

    authors: Gryndler M,Soukupová L,Gryndlerová H,Baldrian P,Hršelová H

    更新日期:2010-09-01 00:00:00

  • Subcellular shifts of trimeric G-proteins following activation of baker's yeast by glucose.

    abstract::Addition of glucose to a resting cell suspension of the yeast Saccharomyces cerevisiae was accompanied by marked shifts of the G alpha-protein subunits from the plasma membrane to the cell interior. This process was rapid with half-times between < 10 and 20 s. The decrease of the plasma membrane pool of the Gi alpha/G...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02814427

    authors: Kotyk A,Ihnatovych I,Lapathitis G,Lamash N,Svoboda P

    更新日期:2001-01-01 00:00:00

  • Gram staining with an automatic machine.

    abstract::This study was undertaken to develop a new Gram-staining machine controlled by a micro-controller and to investigate the quality of slides that were stained in the machine. The machine was designed and produced by the authors. It uses standard 220 V AC. Staining, washing, and drying periods are controlled by a timer b...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02818557

    authors: Felek S,Arslan A

    更新日期:1999-01-01 00:00:00

  • Energy efficiency of different mechanistic models for potassium ion uptake in lower eukaryotic cells.

    abstract::Different mechanistic models for potassium ion uptake are analyzed by an equilibrium-thermodynamic formalism in terms of their comparative efficiency in setting chemical potential differences of the potassium ion of different magnitudes across the plasma membrane of lower eukaryotic cells. The possible adaptive advant...

    journal_title:Folia microbiologica

    pub_type: 杂志文章,评审

    doi:10.1007/BF02925852

    authors: Villalobo A

    更新日期:1988-01-01 00:00:00

  • Characterization and some reaction-engineering aspects of thermostable extracellular beta-galactosidase from a new Bacillus species.

    abstract::A new strain of Bacillus sp. was isolated from a hot water spring in India. This strain generated a high activity of extracellular beta-galactosidase at 37 degrees C in shake flasks. The beta-galactosidase activity was found to increase continuously but the production rate was slower than with some other organisms rep...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02903706

    authors: Sani RK,Chakraborti S,Sobti RC,Patnaik PR,Banerjee UC

    更新日期:1999-01-01 00:00:00

  • Effects of N,N-bis(3-aminopropyl)dodecylamine on antioxidant enzyme activities, mitochondrial morphology and metabolism in Aspergillus niger.

    abstract::The activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSP) as well as of succinate dehydrogenase (SDG), NADH dehydrogenase (NDG) and fumarate hydratase (FHT) were examined in relation to mitochondrial ultrastructure changes in Aspergillus niger exposed to N,N-bis(3-aminopropyl)dodecy...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02931448

    authors: Kuźniak E,Wyrwicka A,Gabara B,Koziróg A,Skłodowska M

    更新日期:2006-01-01 00:00:00

  • Effect of paraquat on cellular defense enzymes and glutathione level of Funalia trogii.

    abstract::The effect of paraquat on the activities of antioxidant defense and detoxifying enzymes of the white-rot fungus Funalia trogii was determined. Paraquat increased the activities of glutathione reductase (GR), glutathione transferase (GT) and superoxide dismutase at 1 mmol/L, while at 0.1 mmol/L it did not affect the ac...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02818700

    authors: Asma D,Yeşilada O

    更新日期:2002-01-01 00:00:00

  • Effect of incubation temperature on acid production and proteolytic activity in milk by selected lactobacilli mutants.

    abstract::Lactobacillus bulgaricus 59 and L. casei RTS and their respective nitrosoguanidine-induced mutants were estimated for titratable and volatile acidities, production of biacetyl and acetoin and for proteolytic activity. Both the mutants showed a marked increase in proteolytic activity as compared to the parents. ...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02897216

    authors: Singh J,Ranganthan B,Chander H

    更新日期:1980-01-01 00:00:00

  • Recombinant single-chain Fv antibodies that recognize the p25 protein of the Maedi-Visna virus.

    abstract::Single chain Fv (scFv) antibodies (generated by phage display technology, molecules representing new and efficient tools in the research and diagnostics of infectious diseases) against the capsid protein (p25) of Maedi-Visna virus were selected. Several clones of p25 specific scFv antibodies were identified; one of th...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02931380

    authors: Celer V,Blazek D,Navrátilová I,Skládal P,Blacklaws B,Bujdoso R

    更新日期:2003-01-01 00:00:00

  • Physiology of Anabaena khannae and Chlorococcum humicola under fluoride stress.

    abstract::Sodium fluoride showed pH-dependent physiological responses in the two test microalgae Anabaena khannae and Chlorococcum humicola. A. khannae showed severe membrane damage with fluoride at low pH with leakage of pigments and electrolytes. Annihilation of photosynthesis along with inhibition in 14C uptake was observed ...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02931045

    authors: Bhatnagar M,Bhatnagar A

    更新日期:2004-01-01 00:00:00

  • Identification of an elongation factor 1Bγ protein with glutathione transferase activity in both yeast and mycelial morphologies from human pathogenic Blastoschizomyces capitatus.

    abstract::Blastoschizomyces capitatus is an uncommon, opportunistic pathogenic fungus, which causes invasive and disseminated infections. This microorganism is normally present in both environmental and normal human flora. Within a host, B. capitatus is able to grow in both unicellular yeast and multicellular filamentous growth...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-013-0273-3

    authors: Allocati N,Masulli M,Del Boccio P,Pieragostino D,D'Antonio D,Sheehan D,Di Ilio C

    更新日期:2014-03-01 00:00:00

  • Peptides representing the specific variable regions but not the full porin proteins can be useful for antibody based diagnosis of typhoid fever.

    abstract::Antibody response to major porin proteins of S. Typhi (OmpC and OmpF) was evaluated in sera of typhoid patients (culture positive, n = 28; Widal positive, n = 16). Sera from fever patients (n = 6) having etiology other than Salmonella, and normal healthy human controls (n = 18) were also included. No significant diffe...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-010-0086-6

    authors: Kumar S,Kapil S,Gautam V,Verma SK,Ray P

    更新日期:2010-09-01 00:00:00

  • Molecular characterization of plasmid pMoma1of Moraxella macacae, a newly described bacterial pathogen of macaques.

    abstract::We report the complete nucleotide sequence and characterization of a small cryptic plasmid of Moraxella macacae 0408225, a newly described bacterial species within the family Moraxellaceae and a causative agent of epistaxis in macaques. The complete nucleotide sequence of the plasmid pMoma1 was determined and found to...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-014-0364-9

    authors: Whitehouse CA,Ladner JT,Palacios GF

    更新日期:2015-05-01 00:00:00

  • Molecular diversity analysis of Rhizoctonia solani isolates infecting various pulse crops in different agro-ecological regions of India.

    abstract::Genetic diversity of 89 isolates of Rhizoctonia solani isolated from different pulse crops representing 21 states from 16 agro-ecological regions of India, 49 morphological, and 7 anastomosis groups (AGs) was analyzed using 12 universal rice primers (URPs), 22 random amplified polymorphic DNA (RAPD), and 23 inter-simp...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-012-0165-y

    authors: Dubey SC,Tripathi A,Upadhyay BK

    更新日期:2012-11-01 00:00:00

  • PCR method for generating multiple mutations at adjacent sites.

    abstract::Procedures to introduce point mutations, restriction sites and insert or delete DNA fragments are very important tools to study protein function. We describe here two-step PCR-based method for generating single or multiple mutations, insertions and deletions in a small region of the sequence. In the first step, a uniq...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02816213

    authors: Adamec J,Kalousek F

    更新日期:1999-01-01 00:00:00

  • Lectin-binding ability of immunoglobulin E and its participation in triggering of mast cells.

    abstract::Eleven purified lectins were compared in their IgE-precipitating, mast-cell-degranulating and histamine-releasing activity and in eliciting skin allergic reactions in atopic or normal people. A significantly positive relationship could be proved between their IgE-precipitating activity and degranulation of IgE-sensiti...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02923516

    authors: Zavázal V,Krauz V

    更新日期:1985-01-01 00:00:00

  • Characterization of Candida albicans colony morphological mutants and their hybrids by means of RAPD-PCR, isoenzyme analysis and pathogenicity analysis.

    abstract::A wild-type strain of Candida albicans (S1, ATCC 10261) was used to obtain stable auxotrophic colony morphological mutants (mutant M5 producing only true hyphae and mutant M2 containing 90 % blastospores and 10 % pseudohyphae) by induced mutagenesis. A hybrid was produced by somatic hybridization between these 2 mutan...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02931528

    authors: Novák A,Vágvölgyi C,Emody L,Pesti M

    更新日期:2004-01-01 00:00:00

  • How much do we know about hemolytic capability of pathogenic Candida species?

    abstract::Hemolytic factor production by pathogenic Candida species is considered an important attribute in promoting survival within the mammal host through the ability to assimilate iron from the hemoglobin-heme group. Hemolytic capability has been evaluated for Candida species based on hemolysis zones on plate assay, analysi...

    journal_title:Folia microbiologica

    pub_type: 杂志文章,评审

    doi:10.1007/s12223-018-0584-5

    authors: Furlaneto MC,Góes HP,Perini HF,Dos Santos RC,Furlaneto-Maia L

    更新日期:2018-07-01 00:00:00

  • Growth of moderately halophilic bacteria isolated from sea water using phenol as the sole carbon source.

    abstract::Moderately halophilic bacteria utilizing phenol as the sole carbon source were isolated by selective enrichment from sea water. The isolate (Gram-negative motile rods) was identified as Deleya venusta. It grew well in the presence of up to 1600 mg/L of phenol and 8% NaCl under aerobic conditions. When the cells were t...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02815617

    authors: Muñoz JA,Pérez-Esteban B,Esteban M,de la Escalera S,Gómez MA,Martínez-Toledo MV,González-López J

    更新日期:2001-01-01 00:00:00

  • Phytoplankton successions under ice cover in four lakes located in north-eastern Sweden: effects of liming.

    abstract::Phytoplankton successions under ice cover (January-March) were determined in four oligotrophic lakes (Burtjärn, Aspen, Vialamptjärn and Storkorstjärn) located in North-Eastern Sweden. The total phosphorus concentration in the lakes was less than 10 micrograms/L. Lake Burtjärn (reference lake) had a similar hydrology a...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02931370

    authors: Wahlström G,Danilov RA

    更新日期:2003-01-01 00:00:00