Abstract：
:Antibody response to major porin proteins of S. Typhi (OmpC and OmpF) was evaluated in sera of typhoid patients (culture positive, n = 28; Widal positive, n = 16). Sera from fever patients (n = 6) having etiology other than Salmonella, and normal healthy human controls (n = 18) were also included. No significant difference between the anti-OmpC and anti-OmpF antibodies (Ab) of typhoid patients and controls was observed. The amino acid sequences of OmpC (and OmpF) porin of enterobacteria was aligned and searched for the variable regions specific to S. Typhi. Two regions, each representing one specific variable region of OmpC and OmpF, were selected (peptides for these regions were custom synthesized). The peptides were evaluated for Ab response of sera. A significantly higher level of Ab to both the peptides was observed in the sera of typhoid patients. The findings suggest that porins of S. Typhi are cross reactive and are not good markers for Ab-based diagnosis of typhoid fever, however, peptides representing the variable regions specific to S. Typhi may have greater diagnostic potential.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Kumar S,Kapil S,Gautam V,Verma SK,Ray Pdoi
10.1007/s12223-010-0086-6subject
Has Abstractpub_date
2010-09-01 00:00:00pages
520-7issue
5eissn
0015-5632issn
1874-9356journal_volume
55pub_type
杂志文章abstract：:The production of bacterial enzymes in batch fermentations is compared with results obtained in continuous culture. When studying the production of alpha-amylase in Bacillus subtilis it was found that instability of the enzyme synthesis was due to nonhomogeneity of the population rather than to "the culture's history"...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02883137
更新日期：1982-01-01 00:00:00
abstract：:Hemolytic factor production by pathogenic Candida species is considered an important attribute in promoting survival within the mammal host through the ability to assimilate iron from the hemoglobin-heme group. Hemolytic capability has been evaluated for Candida species based on hemolysis zones on plate assay, analysi...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/s12223-018-0584-5
更新日期：2018-07-01 00:00:00
abstract：:Candida bracarensis is an emerging cryptic species within the Candida glabrata clade. To date, little is known about its epidemiology, virulence, and antifungal susceptibility. This study documents the occurrence of C. bracarensis for the first time in Mexico and focuses on its in vitro production of hydrolytic enzyme...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-018-0592-5
更新日期：2018-07-01 00:00:00
abstract：:Biofilms represent mixed communities present in a diverse range of environments; however, their utility as inoculants is less investigated. Our investigation was aimed towards in vitro development of biofilms using fungal mycelia (Trichoderma viride) as matrices and nitrogen-fixing and P-solubilizing bacteria as partn...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-012-0154-1
更新日期：2012-09-01 00:00:00
abstract：:A new simple method used to eliminate polysaccharides that cause problems during DNA isolation was established for 6 different white-rot fungi using 1% hexadecyltrimethylammonium bromide (CTAB) as wash buffer and followed by centrifugation. Variation in the DNA yield and quality was ascertained using precipitating age...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931383
更新日期：2004-01-01 00:00:00
abstract：:A selection of tert-butylhydroperoxide (tBOOH)-tolerant Candida albicans mutants showed increased tolerances to 19 different stress conditions. These mutants are characterized by a constitutively upregulated antioxidative defense system and, therefore, adaptation to oxidative stress may play an important role in gaini...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-014-0305-7
更新日期：2014-07-01 00:00:00
abstract：:Bifidobacteria (246 strains in total) were isolated from rectal samples of infants and adult humans and animals, and from intestinal samples of calves. Twenty-five strains grew well on mucin: 20 from infants, two from adults, and three from goatlings. Poor or no growth on mucin was observed in 156 bifidobacterial stra...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0022-4
更新日期：2011-03-01 00:00:00
abstract：:A quarantine organism, "Candidatus Phytoplasma mali," is the causal agent of apple proliferation, one of the most important apple diseases in Europe. The genetic diversity of this pathogen in Central and Southern Europe has already been reported; however, almost no data exists from Eastern Europe. In this study, "Ca. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-018-0660-x
更新日期：2019-05-01 00:00:00
abstract：:Sodium fluoride showed pH-dependent physiological responses in the two test microalgae Anabaena khannae and Chlorococcum humicola. A. khannae showed severe membrane damage with fluoride at low pH with leakage of pigments and electrolytes. Annihilation of photosynthesis along with inhibition in 14C uptake was observed ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931045
更新日期：2004-01-01 00:00:00
abstract：:One hundred clinical isolates of Pseudomonas aeruginosa were checked for their sensitivity towards silver nitrate. Majority of the isolates were resistant at 20 mg/L and the resistance decreased with increasing concentration of silver nitrate, only 5% of the organisms showed resistance above 70 mg/L. These silver-resi...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02820752
更新日期：1989-01-01 00:00:00
abstract：:Lengths of permuted regions in the P22 and L phage genomes were estimated from the relative yields of DNA in many electrophoretic bands obtained using several restriction endonucleases. It was found that 3.6 kb (8.7%) of P22-DNA and 7.2 kb (17.8%) of L-DNA were circularly permuted. In both phages the sequential packag...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02933145
更新日期：1992-01-01 00:00:00
abstract：:Superoxide dismutases, both cytosolic Cu, Zn-SOD encoded by SOD1 and mitochondrial Mn-SOD encoded by SOD2, serve Saccharomyces cerevisiae cells for defense against the superoxide radical but the phenotypes of sod1A and sod2delta mutant strains are different. Compared with the parent strain and the sod1delta mutant, th...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02932150
更新日期：2007-01-01 00:00:00
abstract：:The effect of the newly synthesized phosphonic compound dibutyl 2-octylamino-2-propanephosphonate (DBOP) on the growth of the aquatic plant Spirodela oligorrhiza and stability of red blood cells (RBC) and planar lipid membranes (BLM) was studied to determine its physiological activity and, if possible, correlate this ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02908944
更新日期：2000-01-01 00:00:00
abstract：:The plasmid R1drd-19 Km- and its derivative pON5300 were split by the PstI restriction enzyme; they differed in a 2.4 Mg/mol fragment which was present only in the pON5300. Both plasmids were present in 1-2 copies per chromosomal equivalent in E. coli JC5455 cells. The pON5300 is stable in its original mutagenized hos...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02879554
更新日期：1983-01-01 00:00:00
abstract：:A 6.5 kb DNA fragment containing the gene (thrC) encoding threonine synthase, the last enzyme of the threonine biosynthetic pathway, has been cloned from the DNA of Bacillus sp. ULM1 by complementation of Escherichia coli and Brevibacterium lactofermentum thrC auxotrophs. Complementation studies showed that the thrB g...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818515
更新日期：1995-01-01 00:00:00
abstract：:Composition of sterol fractions of nystatin-resistant Candida maltosa strains was determined. Using UV-spectrometry, TLC and GLC-MS it was demonstrated that resistance to nystatin is connected with the composition alterations of yeast cell sterols. Block of different stages of ergosterol biosynthesis was revealed in s...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814494
更新日期：1991-01-01 00:00:00
abstract：:The effects of pH on denitrifying enzyme activity (DEA) and on the ratio of the denitrification products, N(2)O and N(2), were determined in three pasture soils differing in cattle impact. The linkage between intrinsic differences in the denitrifying communities and pH effects on relative N(2)O production was also ass...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0045-x
更新日期：2011-05-01 00:00:00
abstract：:The ability of surfactants obtained from three Lactobacillus acidophilus strains to inhibit Staphylococcus aureus and S. epidermidis biofilms was evaluated. Their influence was determined on bacterial initial adhesion, biofilm formation and dispersal using MTT-reduction assay, confocal laser scanning microscopy and im...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-008-0009-y
更新日期：2008-01-01 00:00:00
abstract：:White-rot fungi, Coriolus versicolor and Funalia trogii, produced laccase on media with diluted olive-oil mill wastewater and vinasse. Addition of spent cotton stalks enhanced the laccase activity with a maximum after 12 d of cultivation. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02873591
更新日期：2001-01-01 00:00:00
abstract：:The microbial fuel cells (MFCs) are recognized to be highly effective for the biodegradation of phenol. For isolating the phenol-degrading bacteria, the sample containing 500 mg/L phenol was collected from the MFCs. The strain (WL027) was identified basing on the 16S rRNA gene analysis and phylogenetic analysis as Bac...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-017-0536-5
更新日期：2018-01-01 00:00:00
abstract：:A phosphate solubilizing fungus, Aspergillus awamori S29 was isolated from rhizoshpere of mungbean. The phosphate solubilizing activity of A. awamori S29 in liquid was 1,110 mg/L for tricalcium phosphate (TCP). The organism was able to solubilize various inorganic forms of phosphate at a wide range of temperatures. Am...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-012-0167-9
更新日期：2012-11-01 00:00:00
abstract：:Aeromonads represent bacteria thought to be primarily mostly autochthonous to aquatic environments. This study was focused on the relation with antibiotics and enterocins of identified Aeromonas species isolated from the intestine of trouts living in Slovakian aquatic sources. Intestinal samples from 50 trouts (3 Salm...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-018-0616-1
更新日期：2018-11-01 00:00:00
abstract：:Four in vitro experiments were set up to verify the colonization potential of ectomycorrhizal (EcM) Cenococcum geophilum FR. (strain CGE-4), saprotrophic Geomyces pannorum (LINK) SIGLER & CARMICHAEL (GPA-1) and a frequent root-associated, potentially ericoid mycorrhiza (ErM)-forming Meliniomyces variabilis Hambleton &...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02932096
更新日期：2007-01-01 00:00:00
abstract：:In this study, a method combining Raman spectroscopy with chemometric analysis was developed for detection of phage presence in raw milk and discrimination of Streptococcus thermophilus and Lactobacillus bulgaricus phages which are among the main phages causing problems in dairy industry. For this purpose, S. thermoph...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-018-0604-5
更新日期：2018-09-01 00:00:00
abstract：:Cultures of 33 basidiomycete strains out of 35 tested were viable with unchanged characteristics after four years of maintenance on perlite in cryovials. These cultures can be a good substitute for agar cultures in long-term maintenance of fungi. For comparison, the storage under oil was evaluated but it turned out to...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-008-0084-0
更新日期：2008-01-01 00:00:00
abstract：:Investigation of the dependence of cell yield of Candida boidinii in a chemostat and specific growth rate in a turbidostat on temperature was carried out using methanol as substrate. Each of the curves obtained had only one maximum, the optimum temperature for yield (Ys) and for specific growth rate (micron) being som...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02885600
更新日期：1977-01-01 00:00:00
abstract：:The aim of the study was to compare the phenotype of lymphocyte subpopulations of the GALT (gut-associated lymphatic tissue) in germfree (GF) and conventionally (CV) reared rats, i.e. to analyze the effect of microbial colonization on the development of intestinal lymphocyte subsets. Surface marker characteristics wer...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02820814
更新日期：1998-01-01 00:00:00
abstract：:Poly-gamma-L-glutamic acid was synthesized in optimized fermentor cultivation of Bacillus licheniformis. The polypeptide obtained was esterified using benzyl- and butylbromides. Benzyl esters with 30, 60 and 100% degree of esterification were prepared. Solubility of the products in dimethyl sulfoxide, chloroform, meth...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818530
更新日期：2001-01-01 00:00:00
abstract：:Verotoxin-producing Escherichia coli (VTEC) are nowadays among the most important emerging group of food-borne pathogens (VTEC strains cause gastroenteritis that can be complicated by the hemorrhagic colitis or hemolytic uremic syndrome, HUS). Escherichia coli 026 producing verotoxin 2 was isolated and its identity co...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931454
更新日期：2005-01-01 00:00:00
abstract：:The purpose of this study was to isolate bioluminescent strains and to phenotypically and biochemically identify them based on the 16S rRNA gene sequence. 16S rRNA gene sequence analysis of the 11 isolates revealed that they belonged to Shewanella woodyi. Nevertheless, they were determined to exhibit various growth ch...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-013-0269-z
更新日期：2014-01-01 00:00:00
