Abstract：
:Blastoschizomyces capitatus is an uncommon, opportunistic pathogenic fungus, which causes invasive and disseminated infections. This microorganism is normally present in both environmental and normal human flora. Within a host, B. capitatus is able to grow in both unicellular yeast and multicellular filamentous growth forms. In this study, we obtained in vitro morphological conversion of B. capitatus from yeast-to-mycelial phase to investigate the presence and expression of glutathione transferase (GST) enzymes in both cell forms. A protein with GST activity using the model substrate 1-chloro-2,4-dinitrobenzene was detected in both morphologies and identified by tandem mass spectrometry as a eukaryotic elongation factor 1Bγ (eEF1Bγ) protein, a member of the GST superfamily. No significant difference in GST-specific activity and kinetic constants were observed between mycelial and yeast forms, indicating that eEF1Bγ protein did not show differential expression between the two phases.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Allocati N,Masulli M,Del Boccio P,Pieragostino D,D'Antonio D,Sheehan D,Di Ilio Cdoi
10.1007/s12223-013-0273-3subject
Has Abstractpub_date
2014-03-01 00:00:00pages
107-13issue
2eissn
0015-5632issn
1874-9356journal_volume
59pub_type
杂志文章abstract：:Lactobacillus bulgaricus 59 and L. casei RTS and their respective nitrosoguanidine-induced mutants were estimated for titratable and volatile acidities, production of biacetyl and acetoin and for proteolytic activity. Both the mutants showed a marked increase in proteolytic activity as compared to the parents. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02897216
更新日期：1980-01-01 00:00:00
abstract：:Three methods were used for the isolation of fungi in the present investigation: the dilution-plate method, hair-baiting technique with horse hairs and the "exposed plate" method. Sabouraud's glucose agar at 28 degrees C was also used as isolation medium. 102 species and 2 species varieties belonging to 36 genera were...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02821282
更新日期：1990-01-01 00:00:00
abstract：:The induction of tryptophanase was not affected by rifampicin in the rifampicin-resistant mutant of Vibrio el tor while the antibiotic inhibited the induction of tryptophanase in the normal strain at level of ribonucleic acid and protein synthesis. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02877677
更新日期：1977-01-01 00:00:00
abstract：:Twenty-one healthy bottle-fed infants were screened monthly (1-4 months) for bifidobacteria in their stools. Bifidobacteria were detected by culture and isolates specified by PCR. Alternatively, direct PCR in undiluted fecal suspensions was carried out for detection of bifidobacteria under the cultural detection level...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931594
更新日期：2006-01-01 00:00:00
abstract：:When investigating the effect of aeration on the utilization of a lipid substrate from the cultivation broth and production of salinomycin in Streptomyces albus it was demonstrated that a higher aeration results in a better utilization of soya oil and a higher production of the antibiotic. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02879490
更新日期：1983-01-01 00:00:00
abstract：:Crude lipids isolated from Bacillus firmus, but not from other bacilli, were previously found to induce significant resistance against Listeria monocytogenes infection in mice. In this study, formaldehyde- and heat-killed bacterins of eight Bacillus species and some cellular fractions of B. firmus were prepared and te...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02906811
更新日期：1994-01-01 00:00:00
abstract：:Using both monoclonal and polyclonal antibodies against mammalian plectin (multifunctional protein cross-linking cytoskeletal structures, mainly intermediate filaments, in mammalian cells), several putative isoforms of plectin-like proteins were found in protein extracts from the green alga Chlamydomonas eugametos (Vo...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818794
更新日期：2002-01-01 00:00:00
abstract：:Isolates of Stenotrophomonas maltophilia species display the feature "temperature-dependent susceptibility" (TDS) to antibiotics. Both 30TDS strains (at least 4 times lower value of minimum inhibitory concentration (MIC) of an antibiotic at 30 than at 37 degrees C) and 37TDS strains (at least 4 times lower value of MI...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818682
更新日期：2002-01-01 00:00:00
abstract：:Four in vitro experiments were set up to verify the colonization potential of ectomycorrhizal (EcM) Cenococcum geophilum FR. (strain CGE-4), saprotrophic Geomyces pannorum (LINK) SIGLER & CARMICHAEL (GPA-1) and a frequent root-associated, potentially ericoid mycorrhiza (ErM)-forming Meliniomyces variabilis Hambleton &...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02932096
更新日期：2007-01-01 00:00:00
abstract：:The aim of the study was to compare the phenotype of lymphocyte subpopulations of the GALT (gut-associated lymphatic tissue) in germfree (GF) and conventionally (CV) reared rats, i.e. to analyze the effect of microbial colonization on the development of intestinal lymphocyte subsets. Surface marker characteristics wer...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02820814
更新日期：1998-01-01 00:00:00
abstract：:Ecosystems worldwide are exposed to pollutants connected to the industrial production of pharmaceuticals. The objective of this study was to study the composition and characteristics of the soil microbial communities that had been exposed to long-term selection pressure caused by the industrial production of penicilli...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-020-00819-z
更新日期：2020-12-01 00:00:00
abstract：:Physiological and biochemical properties were tested in 45 isolates of Aspergillus egyptiacus (16 isolates), Emericella nidulans (16) and Aspergillus versicolor (13). The three fungal species exhibited common and similar features. The big similarity between A. egyptiacus and E. nidulans was greater than between A. egy...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814449
更新日期：1994-01-01 00:00:00
abstract：:NaN3 was found to inhibit transformation but not the irreversible binding of donor 3H-DNA in competent cells of the original low-transformable strain Bacillus subtilis 168 trp2. Addition of NaN3 to cells of two mutants Bacillus subtilis HT39 and HT46 with an increased transformability decreased substantially the irrev...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02877669
更新日期：1977-01-01 00:00:00
abstract：:A total of 20 putative strains of Erwinia amylovora originating from 11 samples of host plants with symptoms of fire blight were analyzed in detail using commercial polyclonal antibodies in immunochemical tests. Fourteen strains reacted negatively in all tests; 6 strains reacted positively with a polyclonal antibody f...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931569
更新日期：2005-01-01 00:00:00
abstract：:Streptomyces caelestis DSM 40084 produces two osmolytes, viz. 2-O-(alpha-D-glucopyranosyl)-zeta-glyceric acid (GG) and trehalose. Both compounds were isolated and identified by nuclear magnetic resonance spectroscopy and mass spectrometry. A very sensitive regulation of the cell osmolytes was demonstrated in exponenti...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02932103
更新日期：2007-01-01 00:00:00
abstract：:The emergence of spa types and spa-clonal complexes (CC) among clinical methicillin-resistant Staphylococcus aureus isolates collected from the University Clinical Center in Gdańsk between 2008 and 2009 were investigated. Phage typing was used as the initial screening in the study. The basic set of phages and the addi...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-012-0148-z
更新日期：2012-05-01 00:00:00
abstract：:Verotoxin-producing Escherichia coli (VTEC) are nowadays among the most important emerging group of food-borne pathogens (VTEC strains cause gastroenteritis that can be complicated by the hemorrhagic colitis or hemolytic uremic syndrome, HUS). Escherichia coli 026 producing verotoxin 2 was isolated and its identity co...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931454
更新日期：2005-01-01 00:00:00
abstract：:In yeast, the STB5 gene encodes a transcriptional factor belonging to binuclear cluster class (Zn2Cys6) of transcriptional regulators specific to ascomycetes. In this study, we prepared the Kluyveromyces lactis stb5Δ strain and assessed its responses to different stresses. We showed that KlSTB5 gene is able to complem...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-019-00682-7
更新日期：2019-07-01 00:00:00
abstract：:Mathematical expressions were derived for the dilution rate and concentration of algae determining the maximum biomass production in a semicontinuous algal culture. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02926655
更新日期：1979-01-01 00:00:00
abstract：:Genetic diversity of 89 isolates of Rhizoctonia solani isolated from different pulse crops representing 21 states from 16 agro-ecological regions of India, 49 morphological, and 7 anastomosis groups (AGs) was analyzed using 12 universal rice primers (URPs), 22 random amplified polymorphic DNA (RAPD), and 23 inter-simp...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-012-0165-y
更新日期：2012-11-01 00:00:00
abstract：:Stabilized sewage sludge is applied to agricultural fields and farmland due to its high organic matter content. The aim of this study was to investigate the effects of two types of sludge stabilization, mesophilic anaerobic digestion (MAD) and thermophilic anaerobic digestion (TAD), on bacterial communities in sludge,...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-015-0396-9
更新日期：2015-11-01 00:00:00
abstract：:Fifty Escherichia coli strains isolated from stool samples of 51 healthy children, 143 strains isolated from stool samples of 327 children with diarrhea and 24 strains isolated from stool samples of 21 children with suspected hemolytic uremic syndrome were examined for the presence of Shiga toxin-producing E. coli vir...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02817680
更新日期：2002-01-01 00:00:00
abstract：:Three types of heterogeneous preparations and four types of preparations of polysaccharide nature were obtained in studies aimed at the isolation of active compounds from Aspergillus flavus conidia bearing their biological stimulatory activity. Extraction with trichloroacetic acid at 0 degrees C yielded a preparation ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02927425
更新日期：1981-01-01 00:00:00
abstract：:A quarantine organism, "Candidatus Phytoplasma mali," is the causal agent of apple proliferation, one of the most important apple diseases in Europe. The genetic diversity of this pathogen in Central and Southern Europe has already been reported; however, almost no data exists from Eastern Europe. In this study, "Ca. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-018-0660-x
更新日期：2019-05-01 00:00:00
abstract：:Vancomycin is often used in orthopedic surgery as a local prophylaxis of bacterial infection. The aim of this work was to compare the release of vancomycin and its biologically inactive crystalline degradation products (CDP-1) during in vitro experiments from different types of local antibiotic delivery systems (bone ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-019-00752-w
更新日期：2020-06-01 00:00:00
abstract：:The effect of dental plaque bacteria on LFA-1 beta expression on peripheral blood leukocytes was studied in 20 patients with early-onset periodontitis and in 10 healthy controls. Stimulation of PMN with selected dental plaque bacteria which play a role in the pathogeny of periodontitis significantly increased the expr...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02815697
更新日期：1996-01-01 00:00:00
abstract：:A feeding study was performed to monitor the effect of chitosan intake on the fecal microbiota of ten healthy human subjects. Diversity of microflora was monitored during 8 weeks including 4 weeks of chitosan supplementations. Using denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene amplicons and...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-010-0057-y
更新日期：2010-07-01 00:00:00
abstract：:Eleven purified lectins were compared in their IgE-precipitating, mast-cell-degranulating and histamine-releasing activity and in eliciting skin allergic reactions in atopic or normal people. A significantly positive relationship could be proved between their IgE-precipitating activity and degranulation of IgE-sensiti...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02923516
更新日期：1985-01-01 00:00:00
abstract：:The level of an antibiotic capable of inhibiting the etiological agent at the site of infection is an essential prerequisite for successful antibiotic therapy. In some cases, locally applied antibiotics may compensate for limitations of systemic administration and shorten systemic therapy. We aimed at verifying to wha...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0093-2
更新日期：2012-09-01 00:00:00
abstract：:The postantibiotic effect (PAE) and the postantibiotic sub-MIC effect (PASME) of ciprofloxacin, pefloxacin and amikacin were studied for Salmonella typhimurium and S. enteritidis strains. PAE was induced by 2 x and 4 x MIC of antibiotics studied for 0.5 h. After PAE and PASME their effect on prophage induction of a ly...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02816944
更新日期：1997-01-01 00:00:00
