Recombinant single-chain Fv antibodies that recognize the p25 protein of the Maedi-Visna virus.

Abstract:

:Single chain Fv (scFv) antibodies (generated by phage display technology, molecules representing new and efficient tools in the research and diagnostics of infectious diseases) against the capsid protein (p25) of Maedi-Visna virus were selected. Several clones of p25 specific scFv antibodies were identified; one of them was expressed as a soluble scFv molecule, purified by immobilized metal-affinity chromatography and further characterized by sequencing and determination of the kinetic equilibrium association constant. Sequence analysis showed that the rearranged VL and VH domains of the analyzed scFv clone used sequences from the VL3 family (germline DPL16/VL3.1) and VH1 family (germline VH20), respectively. The kinetic equilibrium association constant was determined as KA = 1.12 +/- 0.52 L/mumol.

journal_title

Folia microbiologica

authors

Celer V,Blazek D,Navrátilová I,Skládal P,Blacklaws B,Bujdoso R

doi

10.1007/BF02931380

keywords:

subject

Has Abstract

pub_date

2003-01-01 00:00:00

pages

435-40

issue

3

eissn

0015-5632

issn

1874-9356

journal_volume

48

pub_type

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