Abstract:
:Single chain Fv (scFv) antibodies (generated by phage display technology, molecules representing new and efficient tools in the research and diagnostics of infectious diseases) against the capsid protein (p25) of Maedi-Visna virus were selected. Several clones of p25 specific scFv antibodies were identified; one of them was expressed as a soluble scFv molecule, purified by immobilized metal-affinity chromatography and further characterized by sequencing and determination of the kinetic equilibrium association constant. Sequence analysis showed that the rearranged VL and VH domains of the analyzed scFv clone used sequences from the VL3 family (germline DPL16/VL3.1) and VH1 family (germline VH20), respectively. The kinetic equilibrium association constant was determined as KA = 1.12 +/- 0.52 L/mumol.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Celer V,Blazek D,Navrátilová I,Skládal P,Blacklaws B,Bujdoso Rdoi
10.1007/BF02931380keywords:
subject
Has Abstractpub_date
2003-01-01 00:00:00pages
435-40issue
3eissn
0015-5632issn
1874-9356journal_volume
48pub_type
杂志文章abstract::A gram-negative bacterium GXGL-4A was originally isolated from maize roots. It displayed nitrogen-fixing (NF) ability under nitrogen-free culture condition, and had a significant promotion effect on cucumber growth in the pot inoculation test. The preliminary physiological and biochemical traits of GXGL-4A were charac...
journal_title:Folia microbiologica
pub_type: 杂志文章
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journal_title:Folia microbiologica
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doi:10.1007/BF02922491
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journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02926824
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abstract::Propionate at concentrations up to 0.05% decreased at 25 degrees C the growth and sporulation of Penicillium aurantiogriseum. The standard size of conidiogenous structures (metulae, phialids) and conidia was diminished. The effect was more pronounced at a higher temperature (30 degrees C). Inhibition of ochratoxin pro...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814202
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abstract::Several amino acids affected the amount of bacitracin produced by Bacillus licheniformis ATCC 10716 and the high-yielding mutant B. licheniformis AL. The possibility of an amino acid control mechanism for bacitracin biosynthesis is discussed. The two strains showed a different response to the addition of some amino ac...
journal_title:Folia microbiologica
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doi:10.1007/BF02926454
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abstract::Streptococcus dysgalactiae subsp. equisimilis (SDSE), belonging to the group C and G streptococci, are human pathogens reported to cause clinical manifestations similar to infections caused by Streptococcus pyogenes. To scrutinize the distribution of gene coding for S. pyogenes virulence factors in SDSE, 255 isolates ...
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doi:10.1007/s12223-018-0595-2
更新日期:2018-09-01 00:00:00
abstract::This study was undertaken to develop a new Gram-staining machine controlled by a micro-controller and to investigate the quality of slides that were stained in the machine. The machine was designed and produced by the authors. It uses standard 220 V AC. Staining, washing, and drying periods are controlled by a timer b...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02818557
更新日期:1999-01-01 00:00:00
abstract::The temperature dependence of fluorescence anisotropy, lifetime and differential tangent of 1,6-diphenyl-1,3,5-hexatriene (DPH) and its polar trimethylammonium derivative (TMA-DPH) were investigated in cytoplasmic membranes of Bacillus subtilis. The fluorescence parameters were compared in the two types of membranes p...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02821406
更新日期:1990-01-01 00:00:00
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journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-015-0390-2
更新日期:2015-11-01 00:00:00
abstract::We have investigated the expression of cyclins, cyclin dependent kinases (CDK), and CDK inhibitors (CKI) at the mRNA level in a panel of small-cell lung cancer (SCLC) cell lines in vitro and in vivo as xenografts in nude mice. The results showed that the cell lines expressed varying amounts of most cyclin and CDK's bu...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02820804
更新日期:1998-01-01 00:00:00
abstract::Inducible pigmentation changes were observed in pigmented strains of Brevibacterium sp. M27 and B. flavum treated with N-methyl-N'-nitro-N-nitrosoguanidine. The highest frequency of induction was reached already at a survival of 30-40% with the maximal yield of 6-10%. As compared with the initial yellow colour, three ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02928007
更新日期:1988-01-01 00:00:00
abstract::In ostriches and pheasants, there is still limited information relating to staphylococci and their properties. Biogenic amines (BAs) are nitrogenous low-molecular-weight substances with biological functions in animals, plants and microorganisms. In this study, we focused on BA production by targeted faecal staphylococ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-017-0492-0
更新日期:2017-05-01 00:00:00
abstract::A naturally deficient thiamine and methionine requiring strain of Bacillus coagulans (Ms 5) accumulates lysine in medium only when exogenous pyridoxine (optimal concentration, 0.1 mu g/ml) are supplied. Threonine exerts an inhibitory effect at higher concentrations but pyridoxine does not. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02877085
更新日期:1975-01-01 00:00:00
abstract::Distribution of microtubules and F-actin in aerobically growing cells of Dipodascus magnusii, belonging to the class Saccharomycetes was analyzed using immunofluorescence microscopy and labeling with rhodamine-tagged phalloidin. A conspicuous system of permanent cytoplasmic microtubules was observed in association wit...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02930952
更新日期:2003-01-01 00:00:00
abstract::A collection of 34 lactococcal strains were characterized using the polymerase chain reaction (PCR) for the acmA gene, and for the 16S rDNA gene, and DNA fingerprinting methods for randomly amplified polymorphic DNA (RAPD) and repetitive extragenic palindrome-PCR (rep-PCR). PCR experiments corroborated the genotypic i...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931464
更新日期:2005-01-01 00:00:00
abstract::The effect of paraquat on the activities of antioxidant defense and detoxifying enzymes of the white-rot fungus Funalia trogii was determined. Paraquat increased the activities of glutathione reductase (GR), glutathione transferase (GT) and superoxide dismutase at 1 mmol/L, while at 0.1 mmol/L it did not affect the ac...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02818700
更新日期:2002-01-01 00:00:00
abstract::Hydroxy, n-saturated, branched, dioic, and unsaturated fatty acids in six freshwater wild cyanobacteria (Chroococcus minutus, Lyngbya ceylanica, Merismopedia glauca, Nodularia sphaerocarpa, Nostoc linckia, and Synechococcus aeruginosus) collected from different lakes and springs of Israel have been identified by gas c...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931279
更新日期:2003-01-01 00:00:00
abstract::Submerged cultures of the low-production strain of Tolypocladium inflatum DSM 63544 formed a mixture of cyclosporins (CS) consisting of CS-A, CS-B, "CS-3" and "CS-4". Glucose, sucrose and maltose were highly favored for biomass production but provided a different physiological state necessary for CS biosynthesis. Not ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02884035
更新日期:1991-01-01 00:00:00
abstract::During cultivation in a minimal medium with glucose Alternaria tenuissima and Aspergillus vesicolor produce constitutively alpha- and beta-glucanases. Fractions of beta-1,3-glucanases exhibiting affinity for laminarin were separated by means of gel filtration chromatography. Two neutral beta-1,3-glucanases with affini...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876394
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abstract::Aliphatic 1,2-alkanolamines inhibited beta-glucanase from Candida utilis. The highest inhibitory effect was observed with 1-dodecylamino-3-chloro-2-propanol (Ki = 1.0 mumol/L) and it was much higher than that of D-glucono-delta-lactone. Simple 1,2-alkanolamines, like 2-aminoethanol or 2-methylaminoethanol, did not exh...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02898763
更新日期:1993-01-01 00:00:00
abstract::Pseudomonas aeruginosa is an opportunistic pathogen that causes serious infections, especially in patients with immunodeficiency. It exhibits multiple mechanisms of resistance, including efflux pumps, antibiotic modifying enzymes and limited membrane permeability. The primary reason for the development of novel therap...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-014-0333-3
更新日期:2015-01-01 00:00:00
abstract::Isolates of Stenotrophomonas maltophilia species display the feature "temperature-dependent susceptibility" (TDS) to antibiotics. Both 30TDS strains (at least 4 times lower value of minimum inhibitory concentration (MIC) of an antibiotic at 30 than at 37 degrees C) and 37TDS strains (at least 4 times lower value of MI...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02818682
更新日期:2002-01-01 00:00:00
abstract::Sporulation was repressed in the parent strain by various carbon sources whereas glucose-resistant mutants were resistant to them but not to glycerol 2-phosphate. Both mycobacillin and dipicolinic acid synthesis were repressed in the parent by some of the compounds tested, viz. glucose, pyruvate and glycerol 2-phospha...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02928000
更新日期:1986-01-01 00:00:00
abstract::Common variable immunodeficiency (CVID) comprises a heterogeneous group of patients with as yet undefined genetic defects. Patients with CVID have in common a decrease in the levels of one or more serum immunoglobulin isotypes and a severe defect in the production of specific antibodies. Typically, the patients suffer...
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/BF02814744
更新日期:1995-01-01 00:00:00
abstract::When investigating the effect of aeration on the utilization of a lipid substrate from the cultivation broth and production of salinomycin in Streptomyces albus it was demonstrated that a higher aeration results in a better utilization of soya oil and a higher production of the antibiotic. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02879490
更新日期:1983-01-01 00:00:00
abstract::The treatment of three patients suffering from chronic bacterial prostatitis who were qualified for an experimental phage therapy protocol managed at the Phage Therapy Unit in Wrocław is described. They had previously been treated unsuccessfully with long-term targeted antibiotics, autovaccines, and laser biostimulati...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-009-0064-z
更新日期:2009-09-01 00:00:00
abstract::Purification of streptococcal extracellular NAD+ nucleosidase is associated with changes of kinetic properties. A high-molecular weight component is required for a full activity of the enzyme. The component is not produced by bacteria and is present in the Todd-Hewitt cultivation medium, the beef-heart extract serving...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876396
更新日期:1980-01-01 00:00:00
abstract::The prevalence of extended-spectrum beta-lactamases (ESBL) was determined among isolates of Escherichia coli (n = 63) isolated from hospitalized (43) and healthy (20) children. Ten isolates (21%) were ESBL-positive for two screening tests, the double disk-synergy test and the Oxoid Combination Disk method. One ESBL-po...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02930963
更新日期:2003-01-01 00:00:00
abstract::The ribosomal stalk is involved directly in the interaction of the elongation factors with the ribosome during protein synthesis. The stalk is formed by a complex of five proteins, four small acidic polypeptides and a larger protein which directly interacts with the rRNA at the GTPase center. In eukaryotes, the acidic...
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/BF02816234
更新日期:1999-01-01 00:00:00