Abstract：
:The chloramphenicol resistance gene from pSa was introduced into T-DNA of pTi T37 of Agrobacterium tumefaciens by cointegration with intermediary plasmid based on pBR322. The resulting intermediary vector was mobilized to A. tumefaciens T37 by conjugative plasmid pRK2. The RK2 plasmid also forms contegrates with pTi due to the Tn3 transposon which was used for the mobilization of modified pTi into plasmid-less A. tumefaciens strain. Transconjugants were selected on the basis of their antibiotic resistance markers and tested for agrocin sensitivity as proof of Ti plasmid transfer. Agrocin sensitivity of tranconjugants together with chloramphenicol resistance was shown to be a sufficient and simple criterion of transfer of modified Ti plasmids. Agrobacterium strains with modified Ti plasmids showed decreased virulence in consequence of the presence of additional borderline sequence inside their T-DNA.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Vlasák J,Hrouda M,Bísková R,Ondrej Mdoi
10.1007/BF02926824subject
Has Abstractpub_date
1986-01-01 00:00:00pages
86-93issue
2eissn
0015-5632issn
1874-9356journal_volume
31pub_type
杂志文章abstract：:Bacteria accumulate high amounts of potassium in the cytoplasm. For studying transport of K+ (with 86Rb as a marker) in bacteria (Staphylococcus aureus 17810S), the cells were depleted of the internal K+ pool by a DNP treatment. Kinetics and energetics of 86Rb transport was assayed with glucose as an exogenous energy ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02816378
更新日期：1998-01-01 00:00:00
abstract：:Procedures to introduce point mutations, restriction sites and insert or delete DNA fragments are very important tools to study protein function. We describe here two-step PCR-based method for generating single or multiple mutations, insertions and deletions in a small region of the sequence. In the first step, a uniq...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02816213
更新日期：1999-01-01 00:00:00
abstract：:Bifidobacteria (246 strains in total) were isolated from rectal samples of infants and adult humans and animals, and from intestinal samples of calves. Twenty-five strains grew well on mucin: 20 from infants, two from adults, and three from goatlings. Poor or no growth on mucin was observed in 156 bifidobacterial stra...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0022-4
更新日期：2011-03-01 00:00:00
abstract：:The screening of 17 SAg genes of S. aureus isolated from the sputum of cystic fibrosis (CF) patients revealed that among 47 genetically different strains, 39 (83 %) carried SAg genes. Superantigens forming enterotoxin gene cluster were detected in 20 strains. The 2nd most common superantigen type was selk detected in ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931628
更新日期：2006-01-01 00:00:00
abstract：:A comparative examination of reduced methyl [MV·](+) and benzyl [BV·](+) viologens (as artificial electron donors for quantitative estimation of the respiratory periplasmic (Nap) and membrane-embedded (Nar) nitrate reductases) using a newly constructed nap mutant strain of Paracocccus denitrificans was done. The activ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0004-6
更新日期：2011-01-01 00:00:00
abstract：:The ribosomal stalk is involved directly in the interaction of the elongation factors with the ribosome during protein synthesis. The stalk is formed by a complex of five proteins, four small acidic polypeptides and a larger protein which directly interacts with the rRNA at the GTPase center. In eukaryotes, the acidic...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02816234
更新日期：1999-01-01 00:00:00
abstract：:Physiological and biochemical properties were tested in 45 isolates of Aspergillus egyptiacus (16 isolates), Emericella nidulans (16) and Aspergillus versicolor (13). The three fungal species exhibited common and similar features. The big similarity between A. egyptiacus and E. nidulans was greater than between A. egy...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814449
更新日期：1994-01-01 00:00:00
abstract：:Mycobacterium microti-infected mouse peritoneal macrophages produced high amounts of prostaglandin E2 (PGE2) and nitric oxide (NO) when activated with interferon-gamma (IFN-gamma). In order to understand the relation between PGE2 and NO production and the expression of interleukin-12 (IL-12), interleukin-10 (IL-10) an...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818541
更新日期：2001-01-01 00:00:00
abstract：:The purpose of this study was to isolate bioluminescent strains and to phenotypically and biochemically identify them based on the 16S rRNA gene sequence. 16S rRNA gene sequence analysis of the 11 isolates revealed that they belonged to Shewanella woodyi. Nevertheless, they were determined to exhibit various growth ch...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-013-0269-z
更新日期：2014-01-01 00:00:00
abstract：:A new metabolite was isolated from the culture of Penicillium rugulosum and its structure was determined from physico-chemical data. Accompanying metabolites skyrin and rugulosin were characterized by UV, IR, CD, mass and NMR spectra. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876598
更新日期：1978-01-01 00:00:00
abstract：:The potentiality of twenty-five fungal species belonging to 14 genera isolated from Egyptian soils to produce mevinolin, a hypocholesterolemic agent, when grown on selected substrates was tested. For the first screening samples of culture filtrates were tested by TLC and the positive results were further estimated by ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02826557
更新日期：1997-01-01 00:00:00
abstract：:Beauveria bassiana is widely studied as an alternative to chemical acaricides in controlling the cattle tick Rhipicephalus microplus. Although its biocontrol efficiency has been proved in laboratory and field scales, there is a need to a better understanding of host interaction process at molecular level related to bi...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-018-0659-3
更新日期：2019-05-01 00:00:00
abstract：:Five arsenic-resistant bacterial strains (designated MP1400, MP1400a, MP1400d, APSLA3, and BPSLA3) were isolated from soils collected at the Alps region (Italy), which showed no contamination by arsenic. Phylogenetic analysis of the 16S rRNA gene sequences assigned them to the genera Pseudomonas and Bacillus. Bacillus...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0010-8
更新日期：2011-01-01 00:00:00
abstract：:Eleven purified lectins were compared in their IgE-precipitating, mast-cell-degranulating and histamine-releasing activity and in eliciting skin allergic reactions in atopic or normal people. A significantly positive relationship could be proved between their IgE-precipitating activity and degranulation of IgE-sensiti...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02923516
更新日期：1985-01-01 00:00:00
abstract：:Contemporary aspects of cerebrospinal fluid analysis are discussed, including the relationship to neuro-infective, autoimmune and other neurological diseases. The actual state of cerebrospinal fluid microbiological and cytological investigation and analysis of cerebrospinal fluid protein fractions are described in det...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02931522
更新日期：2003-01-01 00:00:00
abstract：:Ten thermophilic Gram-positive bacteria were isolated from various soils of Saudi Arabia. The strains are spore-forming rods belonging to the species Bacillus stearothermophilus. The cells are motile, strictly aerobic, catalase and oxidase positive. The sporangia appear to be swollen and their position varies from ter...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02927328
更新日期：1981-01-01 00:00:00
abstract：:Plenty of metagenomic studies have suggested possible associations between microbiome composition and colorectal cancer (CRC). However, these techniques are not economic enough for routine use so far. Therefore, we explored the possibility to detect species associated with colorectal cancer by conventional culture fro...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-020-00807-3
更新日期：2020-12-01 00:00:00
abstract：:The present study was conducted to determine the gene responsible for beta-glucosidase (BGL) production and to generate a full-length complementary DNA (cDNA) of one of the putative BGL genes, which showed a significant expression level when Schizophyllum commune KUC9397 was grown in optimized medium. The relative exp...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-016-0484-5
更新日期：2017-05-01 00:00:00
abstract：:We have investigated the expression of cyclins, cyclin dependent kinases (CDK), and CDK inhibitors (CKI) at the mRNA level in a panel of small-cell lung cancer (SCLC) cell lines in vitro and in vivo as xenografts in nude mice. The results showed that the cell lines expressed varying amounts of most cyclin and CDK's bu...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02820804
更新日期：1998-01-01 00:00:00
abstract：:Inactivation of seven strains derived from Escherichia coli B differing in their capacity to repair damage to their DNA (exc, pol, rec) after irradiation with far (254 nm) and middle and near (300 to 380 and 320--400 nm) UV light was investigated. The same bacterial strains were also used as hosts for the UV-irradiate...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876690
更新日期：1980-01-01 00:00:00
abstract：:The cellular immune response (MIF, E-rosette formation and changes in nucleolar morphology of lymphocytes) was followed as related to age and antigenic stimulation. MIF in healthy infants increased from the 2nd to the 12th week of life as compared with the first week, probably due to BCG vaccination. The total and act...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876400
更新日期：1980-01-01 00:00:00
abstract：:Hydroxy, n-saturated, branched, dioic, and unsaturated fatty acids in six freshwater wild cyanobacteria (Chroococcus minutus, Lyngbya ceylanica, Merismopedia glauca, Nodularia sphaerocarpa, Nostoc linckia, and Synechococcus aeruginosus) collected from different lakes and springs of Israel have been identified by gas c...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931279
更新日期：2003-01-01 00:00:00
abstract：:A rifampicin-resistant variant of two strains of Lactobacillus plantarum, one strain of Pediococcus acidilactici, and one strain of Enterococcus faecium were used for the experimental production of lucerne silage. Laboratory silage without inoculants served as a control. Counts of total anaerobes, total lactic acid ba...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-012-0142-5
更新日期：2012-07-01 00:00:00
abstract：:Using mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine, a number of homoserine auxotrophs have been isolated from a glutamate-producing Arthrobacter globiformis excreting L-lysine in good amounts. For further improvement, mutants resistant to the lysine analog S-(2-aminoethyl)-L-cysteine have been isolated from h...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02935823
更新日期：1991-01-01 00:00:00
abstract：:The aim of the study was to compare the phenotype of lymphocyte subpopulations of the GALT (gut-associated lymphatic tissue) in germfree (GF) and conventionally (CV) reared rats, i.e. to analyze the effect of microbial colonization on the development of intestinal lymphocyte subsets. Surface marker characteristics wer...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02820814
更新日期：1998-01-01 00:00:00
abstract：:A high infective dose of Taxocara canis eggs (2,500 eggs per mouse) induced a partial immunosuppression in mice, manifested by inhibition of the proliferative response of splenic T and B cells to polyclonal activators. A glucan immunomodulator given to infected animals at the beginning of the experiment showed a marke...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02820794
更新日期：1998-01-01 00:00:00
abstract：:We present the results of analysis of membrane phosphoproteomes from individual morphological stages of Streptomyces coelicolor that reflect developmentally dependent heterogeneity and phosphorylation of intrinsic and externally added purified Strepomyces aureofaciens EF-Tu. Fast growing nonpathogenic Mycobacterium sm...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-010-0029-2
更新日期：2010-05-01 00:00:00
abstract：:Phytoplankton successions under ice cover (January-March) were determined in four oligotrophic lakes (Burtjärn, Aspen, Vialamptjärn and Storkorstjärn) located in North-Eastern Sweden. The total phosphorus concentration in the lakes was less than 10 micrograms/L. Lake Burtjärn (reference lake) had a similar hydrology a...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931370
更新日期：2003-01-01 00:00:00
abstract：:Coxiella burnetii (C. burnetii), an intracellular zoonotic bacterium causing Q fever, occurs widely in cattle herds. After invasion of the pregnant uterus and initial localization in the placenta, active C. burnetii infections may spread to the fetus hematogenously or by the amniotic-oral route and thus may cause abor...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-017-0553-4
更新日期：2018-03-01 00:00:00
abstract：:Escherichia coli strain HB101 harboring an expression plasmid bearing calf prochymosin gene under the control of the tac promoter was grown in the presence of IPTG with or without novobiocin at 28 and 40 degrees C, respectively. The differential rates of synthesis of prochymosin inclusions, and, for comparison, of bet...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02815545
更新日期：1998-01-01 00:00:00
