L-lysine production by S-2-aminoethyl-L-cysteine-resistant mutants of Arthrobacter globiformis.

Abstract:

:Using mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine, a number of homoserine auxotrophs have been isolated from a glutamate-producing Arthrobacter globiformis excreting L-lysine in good amounts. For further improvement, mutants resistant to the lysine analog S-(2-aminoethyl)-L-cysteine have been isolated from homoserine auxotrophs. For the three potent mutants tested, White's medium was found to be the best. Glucose, ammonium nitrate and biotin were found to be optimum at 280 mmol/L, 40 mmol/L and 22 nmol/L, respectively. With optimal glucose, ammonium nitrate and biotin, the strain AECrVI yielded 36 g lysine per L in flask culture.

journal_title

Folia microbiologica

authors

Samanta TK,Bhattacharyya R

doi

10.1007/BF02935823

subject

Has Abstract

pub_date

1991-01-01 00:00:00

pages

59-66

issue

1

eissn

0015-5632

issn

1874-9356

journal_volume

36

pub_type

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