Abstract：
:Stabilized sewage sludge is applied to agricultural fields and farmland due to its high organic matter content. The aim of this study was to investigate the effects of two types of sludge stabilization, mesophilic anaerobic digestion (MAD) and thermophilic anaerobic digestion (TAD), on bacterial communities in sludge, including the presence of pathogenic microorganisms. Bacterial community structure and phylogenetic diversity were analyzed in four sewage sludge samples from the Czech Republic. Analysis of 16S ribosomal RNA (rRNA) genes showed that investigated sludge samples harbor diverse bacterial populations with only a few taxa present across all samples. Bacterial diversity was higher in sludge samples after MAD versus TAD treatment, and communities in MAD-treated sludge shared the highest genetic similarities. In all samples, the bacterial community was dominated by reads affiliated with Proteobacteria. The sludge after TAD treatment had considerably higher number of reads of thermotolerant/thermophilic taxa, such as the phyla Deinococcus-Thermus and Thermotogae or the genus Coprothermobacter. Only one operational taxonomic unit (OTU), which clustered with Rhodanobacter, was detected in all communities at a relative abundance >1 %. All of the communities were screened for the presence of 16S rRNA gene sequences of pathogenic bacteria using a database of 122 pathogenic species and ≥98 % identity threshold. The abundance of such sequences ranged between 0.23 and 1.57 % of the total community, with lower numbers present after the TAD treatment, indicating its higher hygienization efficiency. Sequences clustering with nontuberculous mycobacteria were present in all samples. Other detected sequences of pathogenic bacteria included Streptomyces somaliensis, Acinetobacter calcoaceticus, Alcaligenes faecalis, Gordonia spp., Legionella anisa, Bordetella bronchiseptica, Enterobacter aerogenes, Brucella melitensis, and Staphylococcus aureus.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Stiborova H,Wolfram J,Demnerova K,Macek T,Uhlik Odoi
10.1007/s12223-015-0396-9subject
Has Abstractpub_date
2015-11-01 00:00:00pages
531-9issue
6eissn
0015-5632issn
1874-9356pii
10.1007/s12223-015-0396-9journal_volume
60pub_type
杂志文章abstract：:The microbial fuel cells (MFCs) are recognized to be highly effective for the biodegradation of phenol. For isolating the phenol-degrading bacteria, the sample containing 500 mg/L phenol was collected from the MFCs. The strain (WL027) was identified basing on the 16S rRNA gene analysis and phylogenetic analysis as Bac...
journal_title：Folia microbiologica
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journal_title：Folia microbiologica
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更新日期：2018-03-01 00:00:00
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journal_title：Folia microbiologica
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doi：10.1007/BF02883137
更新日期：1982-01-01 00:00:00
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doi：10.1007/s12223-017-0533-8
更新日期：2018-01-01 00:00:00
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abstract：:Treatment of human group C and G streptococci with cyanogen bromide results in solubilization of surface protein G molecules. Strain-to-strain variation in the quantity, molar mass and functional activity of protein G extracted from representative group C and G isolates led to the identification of three structurally ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02825666
更新日期：1999-01-01 00:00:00
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journal_title：Folia microbiologica
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doi：10.1007/s12223-017-0553-4
更新日期：2018-03-01 00:00:00
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journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-012-0175-9
更新日期：2013-01-01 00:00:00
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journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02927425
更新日期：1981-01-01 00:00:00
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journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02927364
更新日期：1981-01-01 00:00:00
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journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02817670
更新日期：2002-01-01 00:00:00
abstract：:Bacterial metabolites with communicative functions could provide protection against stress conditions to members of the same species. Yet, information remains limited about protection provided by metabolites in Bacillus cereus and inter-species. This study investigated the effect of extracellular compounds derived fro...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-016-0487-2
更新日期：2017-05-01 00:00:00
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journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02932132
更新日期：2007-01-01 00:00:00
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journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876394
更新日期：1980-01-01 00:00:00
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journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02926655
更新日期：1979-01-01 00:00:00
abstract：:Biochemical properties of a termostable alkaline phosphatase obtained from the mycelium extract of A. caespitosus were described. The enzyme was purified 42-fold with 32% recovery by DEAE-cellulose and concanavalin A-Sepharose chromatography. The molar mass estimated by Sephacryl S-200 or by 7% SDS-PAGE was 138 kDa an...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931303
更新日期：2007-01-01 00:00:00
abstract：:Some Bacillus subtilis mutants with different levels of homoserine dehydrogenase were described. Strains that do not accumulate methionine have a high homoserine dehydrogenase activity. Low activity was detected in mutants where cell growth was completely inhibited by 0.7 mmol/L methionine. A low concentration of dime...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02884064
更新日期：1991-01-01 00:00:00
abstract：:The effect of subinhibitory concentrations (subMICs) of new organic ammonium salts of four homologous series of alkylammonium bromides (32 compounds) was determined with respect to the induction of lysogenic strain prophage, influence of permeability reactions in a rabbit skin test and cytotoxic changes of monolayers ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02815414
更新日期：1995-01-01 00:00:00
abstract：:Inactivation of seven strains derived from Escherichia coli B differing in their capacity to repair damage to their DNA (exc, pol, rec) after irradiation with far (254 nm) and middle and near (300 to 380 and 320--400 nm) UV light was investigated. The same bacterial strains were also used as hosts for the UV-irradiate...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876690
更新日期：1980-01-01 00:00:00
abstract：:Contemporary aspects of cerebrospinal fluid analysis are discussed, including the relationship to neuro-infective, autoimmune and other neurological diseases. The actual state of cerebrospinal fluid microbiological and cytological investigation and analysis of cerebrospinal fluid protein fractions are described in det...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02931522
更新日期：2003-01-01 00:00:00
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更新日期：2019-11-01 00:00:00
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journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02818976
更新日期：1997-01-01 00:00:00
abstract：:Genome projects produce a huge amount of sequence information. As a result, the focus of genomics research is turning toward deduction of functional information about newly discovered genes. Thus structural genomics paves the way for a new discipline called functional genomics by providing the information required for...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02931546
更新日期：2004-01-01 00:00:00
abstract：:The regulation of gene transcription allows yeast cells to respond properly to changing environmental conditions. Several protein complexes take part in this process. They involve RNA polymerase complexes, chromatin remodeling complexes, mediators, general transcription factors and specific transcriptional regulators....
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/s12223-008-0044-8
更新日期：2008-01-01 00:00:00
abstract：:Enzyme synthesis of tryptophan from indole, pyruvate and ammonium salts was studied using Escherichia coli cells exhibiting a significant tryptophanase activity. In addition to the effect of cultivation medium composition and cultivation conditions, factors affecting the course of the conversion were investigated. Pro...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02820485
更新日期：1990-01-01 00:00:00
abstract：:Different mechanistic models for potassium ion uptake are analyzed by an equilibrium-thermodynamic formalism in terms of their comparative efficiency in setting chemical potential differences of the potassium ion of different magnitudes across the plasma membrane of lower eukaryotic cells. The possible adaptive advant...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02925852
更新日期：1988-01-01 00:00:00
abstract：:Structural and functional properties of prokaryotic DNA methyltransferases are summarized. The different aspects of the role of DNA methylation which influences DNA-protein interaction in restriction and modification of DNA and in mismatch repair, DNA replication and gene expression are discussed. ...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02815658
更新日期：1992-01-01 00:00:00
abstract：:The prevalence of potential virulence markers was determined among the population of Polish Campylobacter jejuni and Campylobacter coli isolates from children, chickens, pigs and dogs. The presence of the flaA, flaB, cdtA, cdtB, cdtC, cdtABC, virB11, and cj0588 genes among 74 C. jejuni and 15 C. coli isolates was dete...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-010-0025-6
更新日期：2010-03-01 00:00:00
abstract：:Inducible pigmentation changes were observed in pigmented strains of Brevibacterium sp. M27 and B. flavum treated with N-methyl-N'-nitro-N-nitrosoguanidine. The highest frequency of induction was reached already at a survival of 30-40% with the maximal yield of 6-10%. As compared with the initial yellow colour, three ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02928007
更新日期：1988-01-01 00:00:00
