Abstract:
:Enzyme synthesis of tryptophan from indole, pyruvate and ammonium salts was studied using Escherichia coli cells exhibiting a significant tryptophanase activity. In addition to the effect of cultivation medium composition and cultivation conditions, factors affecting the course of the conversion were investigated. Production of 32.4 g/L of L-tryptophan was reached after 48 h under optimal conditions.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Zeman R,Plachý J,Bulantová H,Sikyta B,Pavlasová E,Stejskalová Edoi
10.1007/BF02820485subject
Has Abstractpub_date
1990-01-01 00:00:00pages
200-4issue
3eissn
0015-5632issn
1874-9356journal_volume
35pub_type
杂志文章abstract::Secreted aspartyl proteinase (Sap) distribution among different C. albicans isolates was determined using SAP-specific primers in polymerase chain reaction (PCR) assay. SAP1, SAP2, and SAP3 were detected in 13 of 40 (32.5%), SAP4 in 38/40 (95%), SAP5 were detected in 30/40 (75%), SAP6 in 23/40 (57.5%) of C. albicans s...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931422
更新日期:2005-01-01 00:00:00
abstract::The diversity and structure of bacterial and actinobacteral diversity and communities were determined in a metallophytic grassland soil from an upland site in northern England. The community profiles were subjected to multivariate analyses using correspondence and cluster analyses. The total bacterial community divers...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-009-0042-5
更新日期:2009-01-01 00:00:00
abstract::Cultures of 33 basidiomycete strains out of 35 tested were viable with unchanged characteristics after four years of maintenance on perlite in cryovials. These cultures can be a good substitute for agar cultures in long-term maintenance of fungi. For comparison, the storage under oil was evaluated but it turned out to...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-008-0084-0
更新日期:2008-01-01 00:00:00
abstract::Procedures to introduce point mutations, restriction sites and insert or delete DNA fragments are very important tools to study protein function. We describe here two-step PCR-based method for generating single or multiple mutations, insertions and deletions in a small region of the sequence. In the first step, a uniq...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02816213
更新日期:1999-01-01 00:00:00
abstract::The effect of paraquat on the activities of antioxidant defense and detoxifying enzymes of the white-rot fungus Funalia trogii was determined. Paraquat increased the activities of glutathione reductase (GR), glutathione transferase (GT) and superoxide dismutase at 1 mmol/L, while at 0.1 mmol/L it did not affect the ac...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02818700
更新日期:2002-01-01 00:00:00
abstract::Streptomyces caelestis DSM 40084 produces two osmolytes, viz. 2-O-(alpha-D-glucopyranosyl)-zeta-glyceric acid (GG) and trehalose. Both compounds were isolated and identified by nuclear magnetic resonance spectroscopy and mass spectrometry. A very sensitive regulation of the cell osmolytes was demonstrated in exponenti...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02932103
更新日期:2007-01-01 00:00:00
abstract::The purpose of this study was to isolate bioluminescent strains and to phenotypically and biochemically identify them based on the 16S rRNA gene sequence. 16S rRNA gene sequence analysis of the 11 isolates revealed that they belonged to Shewanella woodyi. Nevertheless, they were determined to exhibit various growth ch...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-013-0269-z
更新日期:2014-01-01 00:00:00
abstract::Ten thermophilic Gram-positive bacteria were isolated from various soils of Saudi Arabia. The strains are spore-forming rods belonging to the species Bacillus stearothermophilus. The cells are motile, strictly aerobic, catalase and oxidase positive. The sporangia appear to be swollen and their position varies from ter...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02927328
更新日期:1981-01-01 00:00:00
abstract::The structural gene coding for YompC has been identified in the genome of a pathogenic strain of Yersinia enterocolitica O:9, and was subsequently cloned and sequenced. Detailed alignment of the deduced amino acid sequence showed that YompC is a member of the OmpC porin family with the highest degree of homology to Kl...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02932142
更新日期:2007-01-01 00:00:00
abstract::The temperature dependence of fluorescence anisotropy, lifetime and differential tangent of 1,6-diphenyl-1,3,5-hexatriene (DPH) and its polar trimethylammonium derivative (TMA-DPH) were investigated in cytoplasmic membranes of Bacillus subtilis. The fluorescence parameters were compared in the two types of membranes p...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02821406
更新日期:1990-01-01 00:00:00
abstract::A total of 20 putative strains of Erwinia amylovora originating from 11 samples of host plants with symptoms of fire blight were analyzed in detail using commercial polyclonal antibodies in immunochemical tests. Fourteen strains reacted negatively in all tests; 6 strains reacted positively with a polyclonal antibody f...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931569
更新日期:2005-01-01 00:00:00
abstract::The activities of inorganic pyrophosphatase, thymidine kinase and thymidine phosphorylase were measured in Ter-mutants of E. coli K12 which have a higher or a lower dTTP pool than the parent strain. The levels of inorganic pyrophosphatase and thymidine kinase were changed in the same direction and that of thymidine ph...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02884076
更新日期:1983-01-01 00:00:00
abstract::The ability of surfactants obtained from three Lactobacillus acidophilus strains to inhibit Staphylococcus aureus and S. epidermidis biofilms was evaluated. Their influence was determined on bacterial initial adhesion, biofilm formation and dispersal using MTT-reduction assay, confocal laser scanning microscopy and im...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-008-0009-y
更新日期:2008-01-01 00:00:00
abstract::This study was undertaken to develop a new Gram-staining machine controlled by a micro-controller and to investigate the quality of slides that were stained in the machine. The machine was designed and produced by the authors. It uses standard 220 V AC. Staining, washing, and drying periods are controlled by a timer b...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02818557
更新日期:1999-01-01 00:00:00
abstract::We report a novel application of calcein-acetomethyl ester in flow cytometry for rapid estimation of the number of G+-bacteria in rodent feces (Apodemus sylvaticus and Mus sp.f. muridae). We also use the combined application of flow cytometry and Syto-13 or Sypro Orange staining to count rapidly the total bacterial po...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931538
更新日期:2004-01-01 00:00:00
abstract::White-rot fungi, Coriolus versicolor and Funalia trogii, produced laccase on media with diluted olive-oil mill wastewater and vinasse. Addition of spent cotton stalks enhanced the laccase activity with a maximum after 12 d of cultivation. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02873591
更新日期:2001-01-01 00:00:00
abstract::Using phenol--water extraction, a lipopeptidopolysaccharide complex (LPPS) was isolated from Listeria monocytogenes. Some biological and immunological properties of LPPS were compared with lipopolysaccharide isolated by the same procedure from Salmonella typhimurium. LPPS possesses low pyrogenicity, but the immunologi...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02923515
更新日期:1985-01-01 00:00:00
abstract::The mathematical theory of substrate-induced acidification by microorganisms was based on special formulation of mass and charge conservation laws for distinct species. The strength of the theory was tested on the ethanol-induced acidification by Candida utilis in pure water and in phthalate buffer. We showed that the...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02836618
更新日期:1992-01-01 00:00:00
abstract::An experimental model in white mice, infected with a mildly virulent strain of Klebsiella pneumoniae, was elaborated for studies on local immunity of the respiratory tract. Instillation of klebsiella into the supralaryngeal space of anaesthetised animals proved to be more suitable than the commonly used method of intr...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02879494
更新日期:1983-01-01 00:00:00
abstract::Rabbits, sensitized with M. kansasii, responded by a profound inhibition of migration of macrophages elicited by both antigens: the migration index for homologous antigen was 0.51 in the direct test and 0.65 in the indirect test; for heterologous antigen the indexes were 0.53 and 0.67. However, significant differences...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02877345
更新日期:1980-01-01 00:00:00
abstract::A small-colony forming variant of Escherichia coli with a mutation in the ncf gene was analysed. The alternation of the protein composition in the cytoplasmic membrane and the interaction with K and E group colicins indicated a membrane mutation. The effect of this mutation on some membrane-bound processes, the activi...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02885601
更新日期:1977-01-01 00:00:00
abstract::Spatial distribution of ectomycorrhizae-associated basidiomycetes was determined in oakbirch forest using terminal restriction fragment length polymorphism (T-RFLP) analysis. The data were correlated with actual soil humidity, pH, electric conductivity of the soil extract, absorbance A(465) and A(665) of water and alk...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-010-0076-8
更新日期:2010-09-01 00:00:00
abstract::The aim of the study was to compare the phenotype of lymphocyte subpopulations of the GALT (gut-associated lymphatic tissue) in germfree (GF) and conventionally (CV) reared rats, i.e. to analyze the effect of microbial colonization on the development of intestinal lymphocyte subsets. Surface marker characteristics wer...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02820814
更新日期:1998-01-01 00:00:00
abstract::Five arsenic-resistant bacterial strains (designated MP1400, MP1400a, MP1400d, APSLA3, and BPSLA3) were isolated from soils collected at the Alps region (Italy), which showed no contamination by arsenic. Phylogenetic analysis of the 16S rRNA gene sequences assigned them to the genera Pseudomonas and Bacillus. Bacillus...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-011-0010-8
更新日期:2011-01-01 00:00:00
abstract::Fungal cells of Aureobasidium pullulans ATCC 201253 were immobilized by entrapment in chitosan beads, and the immobilized cells were investigated for their ability to produce the polysaccharide pullulan using batch fermentation. The 1% chitosan-entrapped fungal cells were capable of producing pullulan for two cycles o...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-011-0048-7
更新日期:2011-07-01 00:00:00
abstract::A method is presented for the successful decontamination of sparingly soluble pteridine derivatives by microwave irradiation. The method is nondestructive, rapid and effective in eliminating contamination. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814514
更新日期:1991-01-01 00:00:00
abstract::Mice (strains Balb/c and A/J) received an intravenous infusion of bilirubin for a 1 d period. The infusion was delivered at various phases of the primary reaction; the degree of the immune response was expressed as the number of antibody-forming cells against sheep erythrocytes. Bilirubin infusion during both the indu...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02897214
更新日期:1980-01-01 00:00:00
abstract::A soil streptomycete degraded hair, silk, wool, feather and leather which were collected from solid wastes. The organism was identified taxonomically and designated Streptomyces sp. A956. It degraded leather to the maximum extent and solubilized 35.9% of the total nitrogen, 2.32 mg of glycine equivalent amino nitrogen...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02927178
更新日期:1979-01-01 00:00:00
abstract::Raoultella spp. representatives are Gram-negative capsulated, nonmotile rods. These bacteria are found in the natural environment: plants, water, soil and insects. R. ornithinolytica is one of the three species of Raoultella. R. ornithinolytica is the only species within the genus which has the ability to produce orni...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-015-0390-2
更新日期:2015-11-01 00:00:00
abstract::Manganese peroxidase (MnP) was produced by shallow stationary cultures of Phanerochaete chrysosporium growing on N-limited medium. Decolorization of sulfonphthalein (SP) dyes by MnP was investigated. The MnP activity profile and decolorization of SP dyes was correlated and almost all dyes were decolorized at pH 4.0. T...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931512
更新日期:2003-01-01 00:00:00