PCR method for generating multiple mutations at adjacent sites.

abstract：:Antibody response to major porin proteins of S. Typhi (OmpC and OmpF) was evaluated in sera of typhoid patients (culture positive, n = 28; Widal positive, n = 16). Sera from fever patients (n = 6) having etiology other than Salmonella, and normal healthy human controls (n = 18) were also included. No significant diffe...

journal_title：Folia microbiologica

authors： Kumar S,Kapil S,Gautam V,Verma SK,Ray P

更新日期：2010-09-01 00:00:00

abstract：:The emergence of spa types and spa-clonal complexes (CC) among clinical methicillin-resistant Staphylococcus aureus isolates collected from the University Clinical Center in Gdańsk between 2008 and 2009 were investigated. Phage typing was used as the initial screening in the study. The basic set of phages and the addi...

journal_title：Folia microbiologica

authors： Wiśniewska K,Szewczyk A,Piechowicz L,Bronk M,Samet A,Swieć K

更新日期：2012-05-01 00:00:00

abstract：:Hemolytic factor production by pathogenic Candida species is considered an important attribute in promoting survival within the mammal host through the ability to assimilate iron from the hemoglobin-heme group. Hemolytic capability has been evaluated for Candida species based on hemolysis zones on plate assay, analysi...

journal_title：Folia microbiologica

authors： Furlaneto MC,Góes HP,Perini HF,Dos Santos RC,Furlaneto-Maia L

更新日期：2018-07-01 00:00:00

abstract：:Differences in the effect of volatile and gaseous metabolites of germinating pea seeds on the germination of spores of Mucor racemosus and macroconidia of Fusarium oxysporum are described. Germination of spores of M. racemosus was inhibited by seed metabolites whereas germination of macroconidia of F. oxysporum was st...

journal_title：Folia microbiologica

authors： Catská V

更新日期：1980-01-01 00:00:00

abstract：:The pathogenesis of bacillary dysentery can be studied at different levels of integration of the cellular components that constitute the colonic mucosal barrier. We considered the interaction of Shigella flexneri in three experimental systems that provide complementary information and a scheme of events occurring in h...

journal_title：Folia microbiologica

authors： Sansonetti PJ

更新日期：1998-01-01 00:00:00

abstract：:Ultrastructural changes induced by Rickettsia slovaca standard type (ST) and wild type (WT) were examined during their life cycle in L929 and Vero cells. R. slovaca invaded the cytoplasm of the host cell by phagocytosis on the 1st d p.i. Rickettsiae adhering to the cytoplasmic membrane were engulfed by cellular extens...

journal_title：Folia microbiologica

authors： Boldis V,Strus J,Kocianová E,Tusek-Znidaric M,Stefanidesová K,Spitalská E

更新日期：2009-01-01 00:00:00

abstract：:A new simple method used to eliminate polysaccharides that cause problems during DNA isolation was established for 6 different white-rot fungi using 1% hexadecyltrimethylammonium bromide (CTAB) as wash buffer and followed by centrifugation. Variation in the DNA yield and quality was ascertained using precipitating age...

journal_title：Folia microbiologica

authors： Kuhad RC,Kapoor RK,Lal R

更新日期：2004-01-01 00:00:00

abstract：:The validity of the principle of homeoviscous adaptation for Bacillus subtilis was tested by comparing fluorescence anisotropy (1,6-diphenyl-1,3,5-hexatriene) and electron-spin resonance (16-doxylstearate) measurements carried out in isolated plasma membranes and in phospholipid fractions. The physical measurements we...

journal_title：Folia microbiologica

authors： Svobodová J,Julák J,Pilar J,Svoboda P

更新日期：1988-01-01 00:00:00

abstract：:The purpose of this study was to isolate bioluminescent strains and to phenotypically and biochemically identify them based on the 16S rRNA gene sequence. 16S rRNA gene sequence analysis of the 11 isolates revealed that they belonged to Shewanella woodyi. Nevertheless, they were determined to exhibit various growth ch...

journal_title：Folia microbiologica

authors： Ersoy Omeroglu E,Karaboz I,Sudagidan M

更新日期：2014-01-01 00:00:00

abstract：:Microorganisms in the midgut and rectum of the honeybee were enumerated and characterized. Counts of aerobic microorganisms were distinctly lower than counts of anaerobes (10(5)-10(6) viable cells per g of intestinal content vs. 10(8)-10(9) per g). Total numbers of anaerobic microorganisms were almost identical with t...

journal_title：Folia microbiologica

authors： Kacániová M,Chlebo R,Kopernický M,Trakovická A

更新日期：2004-01-01 00:00:00

abstract：:Pseudomonas aeruginosa is an opportunistic pathogen that causes serious infections, especially in patients with immunodeficiency. It exhibits multiple mechanisms of resistance, including efflux pumps, antibiotic modifying enzymes and limited membrane permeability. The primary reason for the development of novel therap...

journal_title：Folia microbiologica

authors： Kwiatek M,Mizak L,Parasion S,Gryko R,Olender A,Niemcewicz M

更新日期：2015-01-01 00:00:00

abstract：:The effect of subinhibitory concentrations (subMICs) of new organic ammonium salts of four homologous series of alkylammonium bromides (32 compounds) was determined with respect to the induction of lysogenic strain prophage, influence of permeability reactions in a rabbit skin test and cytotoxic changes of monolayers ...

journal_title：Folia microbiologica

authors： Majtánová L,Hostacká A,Majtán V

更新日期：1995-01-01 00:00:00

abstract：:Rumen ciliate protozoa intensively engulf bacteria. However, their ability to utilize murein which is the main polysaccharide of bacterial cell wall has hardly been recognized. The present study concerns the ability of the rumen protozoa Diploplastron affine to digest and ferment murein. The ciliates were isolated fro...

journal_title：Folia microbiologica

authors： Bełzecki G,Miltko R,Kwiatkowska E,Michałowski T

更新日期：2010-07-01 00:00:00

abstract：:The reserves of fossil-based fuels, which currently seem sufficient to meet the global demands, is inevitably on the verge of exhaustion. Contemporary raw material for alternate fuel like biodiesel is usually edible plant commodity oils, whose increasing public consumption rate raises the need of finding a non-edible ...

journal_title：Folia microbiologica

authors： Diwan B,Parkhey P,Gupta P

更新日期：2018-09-01 00:00:00

abstract：:Different mechanistic models for potassium ion uptake are analyzed by an equilibrium-thermodynamic formalism in terms of their comparative efficiency in setting chemical potential differences of the potassium ion of different magnitudes across the plasma membrane of lower eukaryotic cells. The possible adaptive advant...

journal_title：Folia microbiologica

authors： Villalobo A

更新日期：1988-01-01 00:00:00

abstract：:When studying the kinetics of DNA synthesis, growth and cell division in Escherichia coli B/r after irradiation with different doses of UV-radiation (254 nm) we could demonstrate, by means of pulse incorporation of 3H-thymidine, a lag in DNA synthesis after the irradiation. The relative rate of the restored DNA synthe...

journal_title：Folia microbiologica

authors： Kucerová H,Vĕchet B

更新日期：1982-01-01 00:00:00

abstract：:Thirty-four thiosemicarbazones and S-alkyl thiosemicarbazones, and some of their Zn(II) and Pd(II) complexes were obtained and purified to investigate antimicrobial activity. MIC values of the compounds were determined by the disc diffusion method against Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pse...

journal_title：Folia microbiologica

authors： Kizilcikli I,Kurt YD,Akkurt B,Genel AY,Birteksöz S,Otük G,Ulküseven B

更新日期：2007-01-01 00:00:00

abstract：:As phospholipases of mycoplasma species may play a role in the pathogenesis of respiratory tract and urogenital tract diseases Mycoplasma mycoides and Acholeplasma laidlawii were examined as to the production of phospholipase A2 (PLA) and attempts were made to purify and characterize it. Both species produced PLA. The...

journal_title：Folia microbiologica

authors： Bhandari S,Asnani PJ

更新日期：1989-01-01 00:00:00

abstract：:It is proposed that the transcription of the c-2 repressor gene initiates at two promoters, pre and prm, similarly to the transcription of the c-I gene of lambda phage. In the P22 phage, these promoters are defined by mutations of c54 and K5. ...

journal_title：Folia microbiologica

authors： Bezdĕk M,Soska J

更新日期：1975-01-01 00:00:00

abstract：:Vinasse, a recalcitrant waste of the ethanol industry was employed for the production of polyhydroxyalkanoate (PHA) by the extremely halophilic archaeon, Haloarcula marismortui in shake flasks. The PHA was recovered by osmotic lysis of the cells and subsequent purification by sodium hypochlorite and organic solvents. ...

journal_title：Folia microbiologica

authors： Pramanik A,Mitra A,Arumugam M,Bhattacharyya A,Sadhukhan S,Ray A,Haldar S,Mukhopadhyay UK,Mukherjee J

更新日期：2012-01-01 00:00:00

abstract：:Mycobacterium avium paratuberculosis (Map) is a pathogen which causes a chronic progressive granulomatous enteritis known as paratuberculosis or Johne's disease and it primarily affects wild and domestic ruminants. The aim of this research was to examine a flock which consisted of 294 goats and was located in Garfagna...

journal_title：Folia microbiologica

authors： Galiero A,Turchi B,Pedonese F,Nuvoloni R,Cantile C,Colombani G,Forzan M,Cerri D,Bandecchi P,Fratini F

更新日期：2017-11-01 00:00:00

abstract：:Using mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine, a number of homoserine auxotrophs have been isolated from a glutamate-producing Arthrobacter globiformis excreting L-lysine in good amounts. For further improvement, mutants resistant to the lysine analog S-(2-aminoethyl)-L-cysteine have been isolated from h...

journal_title：Folia microbiologica

authors： Samanta TK,Bhattacharyya R

更新日期：1991-01-01 00:00:00

abstract：:Oxidizing and assimilating ability of the yeast Candida utilis 49 was tested with 21 different low-boiling organic compounds which come as components of raw synthetic ethanol. The highest yields of yeast dry weight were obtained with ethanol (72.0%), propanol (48.2%), ethyl acetate (43.4%) and acetic acid (34.2%). To ...

journal_title：Folia microbiologica

authors： Sestáková M

更新日期：1976-01-01 00:00:00

abstract：:One hundred clinical isolates of Pseudomonas aeruginosa were checked for their sensitivity towards silver nitrate. Majority of the isolates were resistant at 20 mg/L and the resistance decreased with increasing concentration of silver nitrate, only 5% of the organisms showed resistance above 70 mg/L. These silver-resi...

journal_title：Folia microbiologica

authors： Vasishta R,Chhibber S,Saxena M

更新日期：1989-01-01 00:00:00

abstract：:Saccharomyces cerevisiae strain MW11 is a temperature-sensitive mutant which exports twenty times more proteins at 37 degrees C than parental or wild-type strains do. To understand the mechanism underlying the protein overexport in the mutant the possibility of an altered cell-wall structure leading to facilitated rel...

journal_title：Folia microbiologica

authors： Alexieva KI,Venkov PV

更新日期：2000-01-01 00:00:00

abstract：:A quarantine organism, "Candidatus Phytoplasma mali," is the causal agent of apple proliferation, one of the most important apple diseases in Europe. The genetic diversity of this pathogen in Central and Southern Europe has already been reported; however, almost no data exists from Eastern Europe. In this study, "Ca. ...

journal_title：Folia microbiologica

authors： Fránová J,Koloniuk I,Lenz O,Sakalieva D

更新日期：2019-05-01 00:00:00

abstract：:Substitution with NO2, Cl, Br and I into position 6 of 2-benzthiazolthiol derivatives increased their antimicrobial efficiency while the SH group is preserved. This increase affected both G+ and G--bacteria, mycobacteria and some fungi. The degree of efficiency of the benzthiazol derivatives is also influenced by the ...

journal_title：Folia microbiologica

authors： Foltínová P,Sutoris V,Blöckinger G,Ebringer L

更新日期：1978-01-01 00:00:00

abstract：:The biological activity and the presence of genes sfp and ituD (surfactin and iturin A) among Bacillus strains isolated from the Amazon basin were determined. Bacillus spp. were tested for hemolytic activity and inhibition of fungal growth by agar plate assays in parallel with PCR for identification of sfp and ituD ge...

journal_title：Folia microbiologica

authors： Velho RV,Medina LF,Segalin J,Brandelli A

更新日期：2011-07-01 00:00:00

abstract：:Four in vitro experiments were set up to verify the colonization potential of ectomycorrhizal (EcM) Cenococcum geophilum FR. (strain CGE-4), saprotrophic Geomyces pannorum (LINK) SIGLER & CARMICHAEL (GPA-1) and a frequent root-associated, potentially ericoid mycorrhiza (ErM)-forming Meliniomyces variabilis Hambleton &...

journal_title：Folia microbiologica

authors： Vohník M,Fendrych M,Albrechtová J,Vosátka M

更新日期：2007-01-01 00:00:00

abstract：:Patients suffering from periodontitis or periodontosis were selected for the study. Further subdivision of these groups was based on the presence or absence of herpes and/or adenoviruses in their oral lymphocytes and epithelial cells. The phagocytic and bactericidal activities of oral leukocytes isolated from the same...

journal_title：Folia microbiologica

authors： Ongrádi J,Sallay K,Kulcsár G

更新日期：1987-01-01 00:00:00