Characterization of phospholipase A2 of mycoplasma species.

Abstract:

:As phospholipases of mycoplasma species may play a role in the pathogenesis of respiratory tract and urogenital tract diseases Mycoplasma mycoides and Acholeplasma laidlawii were examined as to the production of phospholipase A2 (PLA) and attempts were made to purify and characterize it. Both species produced PLA. The purified enzyme was found to be heat-labile, active at alkaline pH, revealing a single band in polyacrylamide gel electrophoresis. Metal ions such as calcium and barium, increased its activity whereas solvents at high concentrations decreased it. It was resistant to surfactants.

journal_title

Folia microbiologica

authors

Bhandari S,Asnani PJ

doi

10.1007/BF02814471

subject

Has Abstract

pub_date

1989-01-01 00:00:00

pages

294-301

issue

4

eissn

0015-5632

issn

1874-9356

journal_volume

34

pub_type

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