Abstract：
:Human immunodeficiency virus type 1 (HIV-1) viral protein R (Vpr) exerts multiple effects on viral and host cellular activities during infection, including induction of the cell cycle G2 arrest, and cell death in both human cells and the fission yeast Schizosaccharomyces pombe. We show that treament of exponential-phase wild-type Vpr-expressing S. pombe cells with a low, subinhibitory concentration (0.15 mmol/L) of hydrogen peroxide and 0.1 mmol/L thiamine significantly increased both cell proliferation and survival rates and decreased the number of elongated G2-arrested cells. Short-term, H2O2-induced adaptive stress increased the survival of the cells while acute stress conditions interrupted the Vpr-mediated death of the cells; however, no changes in cell length or cell phase were detected. The results suggest the importance of the oxidative status of the cells in Vpr-mediated processes. Our findings contribute to the development of a new approach via which to investigate the contribution of Vpr to HIV pathogenesis and to reduce the Vpr-mediated effects in HIV-infected patients.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Antal J,Pesti Mdoi
10.1007/BF02931584subject
Has Abstractpub_date
2006-01-01 00:00:00pages
406-12issue
5eissn
0015-5632issn
1874-9356journal_volume
51pub_type
杂志文章abstract：:A comparative examination of reduced methyl [MV·](+) and benzyl [BV·](+) viologens (as artificial electron donors for quantitative estimation of the respiratory periplasmic (Nap) and membrane-embedded (Nar) nitrate reductases) using a newly constructed nap mutant strain of Paracocccus denitrificans was done. The activ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0004-6
更新日期：2011-01-01 00:00:00
abstract：:Using mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine, a number of homoserine auxotrophs have been isolated from a glutamate-producing Arthrobacter globiformis excreting L-lysine in good amounts. For further improvement, mutants resistant to the lysine analog S-(2-aminoethyl)-L-cysteine have been isolated from h...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02935823
更新日期：1991-01-01 00:00:00
abstract：:Three types of heterogeneous preparations and four types of preparations of polysaccharide nature were obtained in studies aimed at the isolation of active compounds from Aspergillus flavus conidia bearing their biological stimulatory activity. Extraction with trichloroacetic acid at 0 degrees C yielded a preparation ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02927425
更新日期：1981-01-01 00:00:00
abstract：:This study presents the effects of the probiotic and enterocin M-producing strain Enterococcus faecium AL41 on microbiota, phagocytic activity (PA), oxidative stress, performance and biochemical parameters when applied individually or in combination with Eleutherococcus senticosus in rabbits. The novelty of the study ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-015-0423-x
更新日期：2016-03-01 00:00:00
abstract：:Cultures of 33 basidiomycete strains out of 35 tested were viable with unchanged characteristics after four years of maintenance on perlite in cryovials. These cultures can be a good substitute for agar cultures in long-term maintenance of fungi. For comparison, the storage under oil was evaluated but it turned out to...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-008-0084-0
更新日期：2008-01-01 00:00:00
abstract：:This study was undertaken to develop a new Gram-staining machine controlled by a micro-controller and to investigate the quality of slides that were stained in the machine. The machine was designed and produced by the authors. It uses standard 220 V AC. Staining, washing, and drying periods are controlled by a timer b...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818557
更新日期：1999-01-01 00:00:00
abstract：:The addition of a nitrogen (nitrate) and carbon sources (acetate, citrate and fructose) and phosphate deficiency (nitrate medium deficient in phosphate) under unaerated conditions induced akinete differentiation in Anabaena torulosa. Aerated cultures of this organism in these nutrients did not differentiate akinetes. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02817617
更新日期：2000-01-01 00:00:00
abstract：:Moderately halophilic bacteria utilizing phenol as the sole carbon source were isolated by selective enrichment from sea water. The isolate (Gram-negative motile rods) was identified as Deleya venusta. It grew well in the presence of up to 1600 mg/L of phenol and 8% NaCl under aerobic conditions. When the cells were t...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02815617
更新日期：2001-01-01 00:00:00
abstract：:Brefeldin A has been recently characterized to act as an inhibitor of intracellular protein export with profound effects on the structure and function of the Golgi apparatus in animal cells. Manifold activities of the antibiotic (under different names) published in the 1960's and 1970's are reviewed: effects on fungal...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02814572
更新日期：1992-01-01 00:00:00
abstract：:Poly-gamma-L-glutamic acid was synthesized in optimized fermentor cultivation of Bacillus licheniformis. The polypeptide obtained was esterified using benzyl- and butylbromides. Benzyl esters with 30, 60 and 100% degree of esterification were prepared. Solubility of the products in dimethyl sulfoxide, chloroform, meth...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818530
更新日期：2001-01-01 00:00:00
abstract：:Murine herpesvirus 68 (MHV-68) is a natural pathogen that infects murid rodents, which serves as hosts for Ixodes ricinus ticks. For the first time, MHV-68 was detected in immature I. ricinus ticks feeding on Lacerta viridis lizards trapped in Slovakia, which supports the idea that ticks can acquire the virus from fee...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-018-0586-3
更新日期：2018-07-01 00:00:00
abstract：:Glycerol-based medium (BM) with and without the addition of 1 g/L ascorbic acid (Asc) and/or 100 mg/L (+/-)-catechin (Cat) was tested for the storage of three commercial wine yeasts at -20 degrees C. The medium supplemented with Asc was also used to store 706 strains to verify the maintenance of the liquid state. A de...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-009-0036-3
更新日期：2009-01-01 00:00:00
abstract：:A hexokinase preparation was obtained from a Saccharomyces cerevisiae mutant strain deficient in glucosephosphate isomerase (GPI) and mannosephosphate isomerase (MPI) by precipitation with ammonium sulfate. The supernatant fraction corresponding to 40-60 % saturation showed the lowest content in GPI and MPI activity. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02927364
更新日期：1981-01-01 00:00:00
abstract：:The biological activity and the presence of genes sfp and ituD (surfactin and iturin A) among Bacillus strains isolated from the Amazon basin were determined. Bacillus spp. were tested for hemolytic activity and inhibition of fungal growth by agar plate assays in parallel with PCR for identification of sfp and ituD ge...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0056-7
更新日期：2011-07-01 00:00:00
abstract：:The present paper reports a modified method for isolation of lysostaphin--a bacteriolytic agent with specific affinity for staphylococcal cell wall. The proposed purification scheme includes three steps. The first procedure is ultrafiltration through a membrane filter giving a yield of 75.6%. The result of ultrafiltra...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814386
更新日期：1993-01-01 00:00:00
abstract：:During cultivation in a minimal medium with glucose Alternaria tenuissima and Aspergillus vesicolor produce constitutively alpha- and beta-glucanases. Fractions of beta-1,3-glucanases exhibiting affinity for laminarin were separated by means of gel filtration chromatography. Two neutral beta-1,3-glucanases with affini...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876394
更新日期：1980-01-01 00:00:00
abstract：:Sodium fluoride showed pH-dependent physiological responses in the two test microalgae Anabaena khannae and Chlorococcum humicola. A. khannae showed severe membrane damage with fluoride at low pH with leakage of pigments and electrolytes. Annihilation of photosynthesis along with inhibition in 14C uptake was observed ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931045
更新日期：2004-01-01 00:00:00
abstract：:Effects of increasing doses of UV-B radiation on photosynthetic efficiency (PE) and motility in Chlamydomonas reinhardtii were investigated. The exposure time ranged from 10 to 120 min at 0.73 W/m2 UV-B radiation. A decrease in compensation points (CP) was followed by an increase in PE on extending the UV-B treatment ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02817448
更新日期：2000-01-01 00:00:00
abstract：:A case report of ventriculoperitoneal shunt infection caused by Candida lusitaniae in a 6-year-old patient with cerebral astrocytoma and obstructive hydrocephalus is presented briefly with emphasis on the course of antifungal treatment. Seven isolates recovered subsequently from the cerebrospinal fluid were studied re...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0063-8
更新日期：2011-09-01 00:00:00
abstract：:Single chain Fv (scFv) antibodies (generated by phage display technology, molecules representing new and efficient tools in the research and diagnostics of infectious diseases) against the capsid protein (p25) of Maedi-Visna virus were selected. Several clones of p25 specific scFv antibodies were identified; one of th...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931380
更新日期：2003-01-01 00:00:00
abstract：:Fungal cells of Aureobasidium pullulans ATCC 201253 were immobilized by entrapment in chitosan beads, and the immobilized cells were investigated for their ability to produce the polysaccharide pullulan using batch fermentation. The 1% chitosan-entrapped fungal cells were capable of producing pullulan for two cycles o...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0048-7
更新日期：2011-07-01 00:00:00
abstract：:Ovine and caprine scrapie occupies a unique place among animal transmissive spongiform encephalopathies (TSE). It is an object of intensive biomedicinal, ecological and economical studies. Its causative agents are demonstrably associated with the development of TSE in farmed minks, goats and moufflons. Ovine strains o...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02818713
更新日期：2000-01-01 00:00:00
abstract：:Strains of genus Citrobacter (152 isolates from 1950 to 1988 deposited in the Czech National Collection of Type Cultures, Prague) were re-classified using biological and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) methods. One-hundred thirty-six strains (ca. 90 %) were i...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-014-0340-4
更新日期：2015-01-01 00:00:00
abstract：:Excessive use of antibiotics contributes to the selection of resistant bacteria and intestinal colonization with multiresistant pathogens poses a risk factor for subsequent infections. The present study assessed vancomycin-resistant enterococci (VRE) carriage rates in patients admitted to our tertiary care hospital. S...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-019-00751-x
更新日期：2020-06-01 00:00:00
abstract：:Bacillus subtilis strains expressing listeriolysin O (LLO) and simultaneously LLO and p60 protein were constructed. The effect of p60 protein on hemolytic activity and on the invasion of professional phagocytes was demonstrated in the absence of other virulence factors of L. monocytogenes. The hemolytic activity of LL...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931440
更新日期：2005-01-01 00:00:00
abstract：:Genome projects produce a huge amount of sequence information. As a result, the focus of genomics research is turning toward deduction of functional information about newly discovered genes. Thus structural genomics paves the way for a new discipline called functional genomics by providing the information required for...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02931546
更新日期：2004-01-01 00:00:00
abstract：:In this report, Gluconobacter strains were screened for coenzyme Q10 (CoQ10) production. A thermotolerant strain, Gluconobacter japonicus FM10, was eventually employed for CoQ10 production optimization. To do so, a two-step optimization strategy was used. The first step focused on biomass increase and the second step ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-018-0666-4
更新日期：2019-07-01 00:00:00
abstract：:Structural and functional properties of prokaryotic DNA methyltransferases are summarized. The different aspects of the role of DNA methylation which influences DNA-protein interaction in restriction and modification of DNA and in mismatch repair, DNA replication and gene expression are discussed. ...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02815658
更新日期：1992-01-01 00:00:00
abstract：:The screening of 17 SAg genes of S. aureus isolated from the sputum of cystic fibrosis (CF) patients revealed that among 47 genetically different strains, 39 (83 %) carried SAg genes. Superantigens forming enterotoxin gene cluster were detected in 20 strains. The 2nd most common superantigen type was selk detected in ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931628
更新日期：2006-01-01 00:00:00
abstract：:Naturally occurring plant phenolics, p-coumaric acid (PA), caffeic acid (CA), ferulic acid (FA) and gentisic acid (GA) (25-100 nmol/L) had protective effects on acridine orange (AO; 216 mumol/L)- and ofloxacin (3 mumol/L)-induced genotoxicity in Salmonella typhimurium. FA, GA and CA exhibited a significant concentrati...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02817994
更新日期：2001-01-01 00:00:00
