Usefulness of McRAPD for typing and importance of biofilm production in a case of nosocomial ventriculoperitoneal shunt infection caused by Candida lusitaniae.

Abstract:

:A case report of ventriculoperitoneal shunt infection caused by Candida lusitaniae in a 6-year-old patient with cerebral astrocytoma and obstructive hydrocephalus is presented briefly with emphasis on the course of antifungal treatment. Seven isolates recovered subsequently from the cerebrospinal fluid were studied retrospectively. To confirm identity, isolates were typed using pulsed-field gel electrophoresis and melting curve of random amplified polymorphic DNA (McRAPD). Further, the ability to form biofilm and its susceptibility to systemic antifungals were evaluated. Using McRAPD, identity of C. lusitaniae isolates showing slight microevolutionary changes in karyotypes was undoubtedly confirmed; successful application of numerical interpretation of McRAPD for typing is demonstrated here for the first time. The strain was also recognized as a strong biofilm producer. Moreover, minimum biofilm inhibitory concentrations were very high, in contrast to low antifungal minimum inhibitory concentrations of isolates. It can be concluded that McRAPD seems to be a simple and reliable method not only for identification but also for typing of yeasts. A ventriculoperitoneal shunt colonized by C. lusitaniae was revealed as the source of this nosocomial infection, and the ability of the strain to form biofilm on its surface likely caused treatment failure.

journal_title

Folia microbiologica

authors

Hamal P,Hanzen J,Horn F,Trtkova J,Ruskova L,Vecerova R,Ruzicka F,Vollekova A,Raclavsky V

doi

10.1007/s12223-011-0063-8

subject

Has Abstract

pub_date

2011-09-01 00:00:00

pages

407-14

issue

5

eissn

0015-5632

issn

1874-9356

journal_volume

56

pub_type

杂志文章
  • Occurrence of extended-spectrum beta-lactamases among Escherichia coli isolates from hospitalized and healthy children.

    abstract::The prevalence of extended-spectrum beta-lactamases (ESBL) was determined among isolates of Escherichia coli (n = 63) isolated from hospitalized (43) and healthy (20) children. Ten isolates (21%) were ESBL-positive for two screening tests, the double disk-synergy test and the Oxoid Combination Disk method. One ESBL-po...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02930963

    authors: Franiczek R,Sobieszczańska B,Grabowski M,Mowszet K,Pytrus T

    更新日期:2003-01-01 00:00:00

  • Antibiotic resistance of Stenotrophomonas maltophilia strains isolated from captive snakes.

    abstract::The minimum inhibitory concentrations (MICs) of 24 antibiotics were determined for 45 Stenotrophomonas maltophilia strains by the microdilution method at 37 and 30 degrees C (after 24 h and 48 h of incubation). The isolates were obtained from mouth swabs and pus of 116 captive snakes whereas the identical strains (bas...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-010-0014-9

    authors: Hejnar P,Kolár M,Sauer P

    更新日期:2010-01-01 00:00:00

  • The decreased antibacterial activity of oral polymorphonuclear leukocytes coincides with the occurrence of virus-carrying oral lymphocytes and epithelial cells.

    abstract::Patients suffering from periodontitis or periodontosis were selected for the study. Further subdivision of these groups was based on the presence or absence of herpes and/or adenoviruses in their oral lymphocytes and epithelial cells. The phagocytic and bactericidal activities of oral leukocytes isolated from the same...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02887577

    authors: Ongrádi J,Sallay K,Kulcsár G

    更新日期:1987-01-01 00:00:00

  • Lowering of ochratoxin A level in milk by yoghurt bacteria and bifidobacteria.

    abstract::A decrease in the content of ochratoxin A (OA) was observed in milk samples fermented by yoghurt bacteria and bifidobacteria. OA was added to the milk before fermentation at a rate of 0.05-1.5 mg/L. No residues of OA were found in samples containing 0.05 and 0.1 mg/L of OA, fermented by S. Salivarius subsp. thermophil...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02816335

    authors: Skrinjar M,Rasić JL,Stojicić V

    更新日期:1996-01-01 00:00:00

  • Transcriptome analysis of Pseudomonas sp. from subarctic tundra soil: pathway description and gene discovery for humic acids degradation.

    abstract::Although humic acids (HA) are involved in many biological processes in soils and thus their ecological importance has received much attention, the degradative pathways and corresponding catalytic genes underlying the HA degradation by bacteria remain unclear. To unveil those uncertainties, we analyzed transcriptomes e...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-017-0573-0

    authors: Kim D,Park HJ,Sul WJ,Park H

    更新日期:2018-05-01 00:00:00

  • Isolation of Pseudobutyrivibrio ruminis and Pseudobutyrivibrio xylanivorans from rumen of Creole goats fed native forage diet.

    abstract::We isolated and identified functional groups of bacteria in the rumen of Creole goats involved in ruminal fermentation of native forage shrubs. The functional bacterial groups were evaluated by comparing the total viable, total anaerobic, cellulolytic, hemicellulolytic, and amylolytic bacterial counts in the samples t...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-012-0219-1

    authors: Grilli DJ,Cerón ME,Paez S,Egea V,Schnittger L,Cravero S,Escudero MS,Allegretti L,Arenas GN

    更新日期:2013-09-01 00:00:00

  • Dependence of cell yield and specific growth rate of Candida boidinii on temperature during continuous cultivation.

    abstract::Investigation of the dependence of cell yield of Candida boidinii in a chemostat and specific growth rate in a turbidostat on temperature was carried out using methanol as substrate. Each of the curves obtained had only one maximum, the optimum temperature for yield (Ys) and for specific growth rate (micron) being som...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02885600

    authors: Kuvshinnikov VD,Sobotka M,Minkevich IG,Eroshin VK

    更新日期:1977-01-01 00:00:00

  • Bacterial metabolites from intra- and inter-species influencing thermotolerance: the case of Bacillus cereus and Geobacillus stearothermophilus.

    abstract::Bacterial metabolites with communicative functions could provide protection against stress conditions to members of the same species. Yet, information remains limited about protection provided by metabolites in Bacillus cereus and inter-species. This study investigated the effect of extracellular compounds derived fro...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-016-0487-2

    authors: Gómez-Govea MA,García S,Heredia N

    更新日期:2017-05-01 00:00:00

  • Stability and refractoriness of the high catalase activity in the oxidative-stress-resistant fission yeast Schizosaccharomyces pombe.

    abstract::Effect of oxygen and metabolic substrates (glucose, ethanol) on the catalase activity of anaerobically grown Schizosaccharomyces pombe cells was assessed and compared with that of Saccharomyces cerevisiae in order to determine the catalase activity regulation in S. pombe. In contrast to S. cerevisiae, the total catala...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02818575

    authors: Sigler K,Gille G

    更新日期:1998-01-01 00:00:00

  • Mosaic structure of the small cryptic plasmid pKST23 from Escherichia coli.

    abstract::Small plasmid pKST23 was isolated from sheep ruminal Escherichia coli population. Plasmid sequence was determined to be 2,779 bp in length and was found to have an overall 42 % of GC pairs. However, its sequence can be divided into two regions based on genetic composition and the GC content. It was found that the high...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-012-0124-7

    authors: Fecskeová L,Kovařík M,Javorský P,Pristaš P

    更新日期:2012-07-01 00:00:00

  • Comparison of permuted region lengths in the genomes of related Salmonella typhimurium phages P22 and L.

    abstract::Lengths of permuted regions in the P22 and L phage genomes were estimated from the relative yields of DNA in many electrophoretic bands obtained using several restriction endonucleases. It was found that 3.6 kb (8.7%) of P22-DNA and 7.2 kb (17.8%) of L-DNA were circularly permuted. In both phages the sequential packag...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02933145

    authors: Spanová A

    更新日期:1992-01-01 00:00:00

  • Clonal distribution of bone sialoprotein-binding protein gene among Staphylococcus aureus isolates associated with bloodstream infections.

    abstract::Staphylococcus aureus is a leading cause of bloodstream infections (BSI) and diseases that may be caused by hematogenous spread. The staphylococcal adhesin, for which the association with the infections emerging as a complication of septicemia has been well documented, is a bone sialoprotein-binding protein (Bbp). The...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-014-0321-7

    authors: Wiśniewska K,Piórkowska A,Kasprzyk J,Bronk M,Świeć K

    更新日期:2014-11-01 00:00:00

  • Characterization of five newly isolated bacteriophages active against Pseudomonas aeruginosa clinical strains.

    abstract::Pseudomonas aeruginosa is an opportunistic pathogen that causes serious infections, especially in patients with immunodeficiency. It exhibits multiple mechanisms of resistance, including efflux pumps, antibiotic modifying enzymes and limited membrane permeability. The primary reason for the development of novel therap...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-014-0333-3

    authors: Kwiatek M,Mizak L,Parasion S,Gryko R,Olender A,Niemcewicz M

    更新日期:2015-01-01 00:00:00

  • Peptides representing the specific variable regions but not the full porin proteins can be useful for antibody based diagnosis of typhoid fever.

    abstract::Antibody response to major porin proteins of S. Typhi (OmpC and OmpF) was evaluated in sera of typhoid patients (culture positive, n = 28; Widal positive, n = 16). Sera from fever patients (n = 6) having etiology other than Salmonella, and normal healthy human controls (n = 18) were also included. No significant diffe...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-010-0086-6

    authors: Kumar S,Kapil S,Gautam V,Verma SK,Ray P

    更新日期:2010-09-01 00:00:00

  • Modified simplified method for isolation of lysostaphin from the culture filtrate of Staphylococcus staphylolyticus.

    abstract::The present paper reports a modified method for isolation of lysostaphin--a bacteriolytic agent with specific affinity for staphylococcal cell wall. The proposed purification scheme includes three steps. The first procedure is ultrafiltration through a membrane filter giving a yield of 75.6%. The result of ultrafiltra...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02814386

    authors: Márová I,Dadák V

    更新日期:1993-01-01 00:00:00

  • Role of organic acid metabolism in the biosynthesis of peptide ergot alkaloids.

    abstract::Regulatory mechanism of primary metabolism responsible for the biosynthesis of peptide ergot alkaloids was proposed on the basis of experimental results from the production phase of Claviceps purpurea (FR.) Tul. in parasitic and saprophytic cultures. The production-phase metabolism is characterized by uncoupling of gl...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02927365

    authors: Kybal J,Svoboda E,Stranadová K,Kejzlar M

    更新日期:1981-01-01 00:00:00

  • 5-Deoxy-5-fluoro-L-sorbose originating from 2-deoxy-2-fluoro-D-glucitol by fermentation with Acetomonas oxydans.

    abstract::Using fermentation with a selected strain of Acetomonas oxydans it was possible to convert 2-deoxy-2-fluro-D-glucitol to 5-deoxy-5-fluoro-L-sorbose, in agreement with Bertrand's and Hudson's rule. The last-named compound was isolated in a yield of 88%. Both compounds were little toxic against Acetomonas oxydans. ...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02877659

    authors: Kulhánek M,Tadra M,Pacák J,Trejbalová H,Cerný M

    更新日期:1977-01-01 00:00:00

  • Membrane mutation affecting energy-linked functions in Escherichia coli K 12.

    abstract::A small-colony forming variant of Escherichia coli with a mutation in the ncf gene was analysed. The alternation of the protein composition in the cytoplasmic membrane and the interaction with K and E group colicins indicated a membrane mutation. The effect of this mutation on some membrane-bound processes, the activi...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02885601

    authors: Braná H,Hubácek J,Michaljanicová D,Holubová I,Cejka K

    更新日期:1977-01-01 00:00:00

  • Industrial and agricultural wastes as substrates for laccase production by white-rot fungi.

    abstract::White-rot fungi, Coriolus versicolor and Funalia trogii, produced laccase on media with diluted olive-oil mill wastewater and vinasse. Addition of spent cotton stalks enhanced the laccase activity with a maximum after 12 d of cultivation. ...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02873591

    authors: Kahraman S,Yeşilada O

    更新日期:2001-01-01 00:00:00

  • Inhibition of yeast-mycelium transformation by 2-alkylthio-6-amino- and 2-alkylthio-6-formamidobenzothiazoles and their in vitro antifungal activity.

    abstract::The antifungal effect of 2-alkylthio-6-amino- and 2-alkylthio-6-formamidobenzothiazoles (22 derivatives in all) on Aspergillus niger and various Candida yeasts was tested. No significant effect was observed with A. niger. With the pathogenic yeast species C. albicans and C. guilliermondii, the most efficient derivativ...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02814461

    authors: Kuchta T,Bujdáková H,Sidóová E

    更新日期:1989-01-01 00:00:00

  • Establishment and follow-up of bifidobacterial species in the gut of healthy bottle-fed infants of 1-4 months age.

    abstract::Twenty-one healthy bottle-fed infants were screened monthly (1-4 months) for bifidobacteria in their stools. Bifidobacteria were detected by culture and isolates specified by PCR. Alternatively, direct PCR in undiluted fecal suspensions was carried out for detection of bifidobacteria under the cultural detection level...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02931594

    authors: Mullié C,Romond MB,Izard D

    更新日期:2006-01-01 00:00:00

  • Serotypic characterization of group A rotaviruses associated with children's diarrhea in Slovakia.

    abstract::A total of 368 rotavirus RNA-positive (PAGE) stool samples collected continually during 1992-95 from infants and young children under five years of age hospitalized with acute gastroenteritis were serotyped using an enzyme immunoassay with VP7-specific monoclonal antibodies (ELISA with MAbs) for serotypes G1-G4. The s...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02817421

    authors: Tietzová J,Petrovicová A

    更新日期:2000-01-01 00:00:00

  • Antifungal efficacy of bacteria isolated from marine sedentary organisms.

    abstract::The antibiotic-producing ability of 57 bacteria isolated from 8 marine sedentary organisms, 6 sponges (Spirastrella sp., Phyllospongia sp., Ircinia sp., Aaptos sp., Azorica sp., Axinella sp.), 1 soft coral (Lobophytum sp.) and 1 alga (Sargassum sp.), was evaluated against 6 phytopathogenic fungi (Helminthosporium oryz...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02818565

    authors: Mohapatra BR,Bapuji M,Sree A

    更新日期:2002-01-01 00:00:00

  • Virulence factors in clinical and food isolates of Aeromonas species.

    abstract::Virulence factors were compared in 15 Aeromonas spp. isolated from faeces of patients with Aeromonas-associated gastroenteritis and in 81 strains isolated from food. Strains from food did not show differences in the distribution of virulence factors when compared with strains isolated from faeces. However, 88.8% of Ae...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02814323

    authors: Pin C,Marín ML,Selgas MD,García ML,Tormo J,Casas C

    更新日期:1994-01-01 00:00:00

  • The effects of temperature, pH, and iron on biofilm formation by clinical versus environmental strains of Vibrio vulnificus.

    abstract::Due to the nature of Vibrio vulnificus infections (i.e., gastroenteritis and septicemia), only very few studies of a biofilm-associated form in this pathogen's life cycle have been conducted. We proposed that biofilm production by clinical strains of V. vulnificus would be higher than by environmental strains. Biofilm...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-019-00761-9

    authors: Çam S,Brinkmeyer R

    更新日期:2020-06-01 00:00:00

  • Effect of nutrient variations on growth and brefeldin A formation in Curvularia lunata.

    abstract::The highest level of secreted brefeldin A was present in glucose-grown cultures, intermediate levels in glucose-fructose, and xylose cultures and low levels in fructose- and galactose-grown cultures of Curvularia lunata. The biomass decreased from glucose, fructose, xylose, glucose-fructose to galactose cultures. Bref...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02876993

    authors: Sam CT

    更新日期:1977-01-01 00:00:00

  • Characteristics and genetic diversity of bioluminescent Shewanella woodyi strains isolated from the Gulf of Izmir, Turkey.

    abstract::The purpose of this study was to isolate bioluminescent strains and to phenotypically and biochemically identify them based on the 16S rRNA gene sequence. 16S rRNA gene sequence analysis of the 11 isolates revealed that they belonged to Shewanella woodyi. Nevertheless, they were determined to exhibit various growth ch...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-013-0269-z

    authors: Ersoy Omeroglu E,Karaboz I,Sudagidan M

    更新日期:2014-01-01 00:00:00

  • Purification and characterization of two beta-glucosidases from the thermophilic fungus Thermoascus aurantiacus.

    abstract::beta-Glucosidase from the fungus Thermoascus aurantiacus grown on semi-solid fermentation medium (using ground corncob as substrate) was partially purified in 5 steps--ultrafiltration, ethanol precipitation, gel filtration and 2 anion exchange chromatography runs, and characterized. After the first anion exchange chro...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02818672

    authors: de Palma-Fernandez ER,Gomes E,da Silva R

    更新日期:2002-01-01 00:00:00

  • Introducing a thermotolerant Gluconobacter japonicus strain, potentially useful for coenzyme Q10 production.

    abstract::In this report, Gluconobacter strains were screened for coenzyme Q10 (CoQ10) production. A thermotolerant strain, Gluconobacter japonicus FM10, was eventually employed for CoQ10 production optimization. To do so, a two-step optimization strategy was used. The first step focused on biomass increase and the second step ...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-018-0666-4

    authors: Moghadami F,Fooladi J,Hosseini R

    更新日期:2019-07-01 00:00:00

  • Characteristic distribution of immunoglobulin-containing cells in palatine tonsils.

    abstract::Using fluorescein-labelled antibodies against gamma, mu and alpha chains, Ig-containing cells in palatine tonsils were studied in 120 patients. The aim of this study was to determine the most frequently repeated typical findings as regards the numbers and localisation of these cells in tonsils and to confront the data...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02927432

    authors: Franĕk J,Kubín V,Novák V,Bedka J

    更新日期:1981-01-01 00:00:00