Abstract：
:Regulatory mechanism of primary metabolism responsible for the biosynthesis of peptide ergot alkaloids was proposed on the basis of experimental results from the production phase of Claviceps purpurea (FR.) Tul. in parasitic and saprophytic cultures. The production-phase metabolism is characterized by uncoupling of glycolysis from the citric acid cycle and by a break in this cycle at the level of the 2-exoglutarate dehydrogenase complex. This regulation is due to the inhibition by citrate and malate, i.e. the final products continuously taken up from the external medium. The rate of the intact reaction steps of the citric acid cycle is therefore not limited by the actual acetyl-CoA or oxaloacetate pool. The regulation also leads to an excessive synthesis of acetyl-CoA, 2-oxoglutarate and oxaloacetate, which represent the key metabolites of primary metabolism, and to their utilization in the biosynthesis of peptide alkaloids.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Kybal J,Svoboda E,Stranadová K,Kejzlar Mdoi
10.1007/BF02927365subject
Has Abstractpub_date
1981-01-01 00:00:00pages
112-9issue
2eissn
0015-5632issn
1874-9356journal_volume
26pub_type
杂志文章abstract：:A small-colony forming variant of Escherichia coli with a mutation in the ncf gene was analysed. The alternation of the protein composition in the cytoplasmic membrane and the interaction with K and E group colicins indicated a membrane mutation. The effect of this mutation on some membrane-bound processes, the activi...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02885601
更新日期：1977-01-01 00:00:00
abstract：:Inactivation of seven strains derived from Escherichia coli B differing in their capacity to repair damage to their DNA (exc, pol, rec) after irradiation with far (254 nm) and middle and near (300 to 380 and 320--400 nm) UV light was investigated. The same bacterial strains were also used as hosts for the UV-irradiate...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876690
更新日期：1980-01-01 00:00:00
abstract：:A method is presented for the successful decontamination of sparingly soluble pteridine derivatives by microwave irradiation. The method is nondestructive, rapid and effective in eliminating contamination. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814514
更新日期：1991-01-01 00:00:00
abstract：:Biotechnological conversion of low-cost agro-industrial by-products, such as industrial waste or terpenes from the distillation of essential oils from plants into more valuable oxygenated derivatives, can be achieved by using microbial cells or enzymes. In Argentina, the essential oil industry produces several tons of...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-015-0422-y
更新日期：2016-03-01 00:00:00
abstract：:The cellular immune response (MIF, E-rosette formation and changes in nucleolar morphology of lymphocytes) was followed as related to age and antigenic stimulation. MIF in healthy infants increased from the 2nd to the 12th week of life as compared with the first week, probably due to BCG vaccination. The total and act...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876400
更新日期：1980-01-01 00:00:00
abstract：:Using an in vitro cytotoxicity assay (51Cr-release), it was shown that bilirubin exerts a cytotoxic effect on both adult and newborn human lymphocytes after a 1-d incubation of cells with bilirubin. The effect of bilirubin on human granulocytes was less pronounced; the association of the cytotoxic effect with a functi...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02923520
更新日期：1985-01-01 00:00:00
abstract：:Biodegradation of two amphiphilic "soft" antimicrobially active derivatives of lauric (dodecanoic) acid, a quaternary ammonium salt and an amine oxide bearing an amide or ester group, was followed using microorganisms from activated sludge. Primary biodegradation was determined by ion-selective electrodes, total biode...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814548
更新日期：1993-01-01 00:00:00
abstract：:The resistance to killing by free radicals of two mutants ofPhaffia rhodozyma was determined. Mutant 5-7 did not produce astaxanthin but produced beta-carotene, while mutant 3-4 did not produce any carotenoid pigments. The resistance of mutant 5-7 was the same as that of the wild type but mutant 3-4 was rapidly killed...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02816505
更新日期：1998-01-01 00:00:00
abstract：:The activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSP) as well as of succinate dehydrogenase (SDG), NADH dehydrogenase (NDG) and fumarate hydratase (FHT) were examined in relation to mitochondrial ultrastructure changes in Aspergillus niger exposed to N,N-bis(3-aminopropyl)dodecy...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931448
更新日期：2006-01-01 00:00:00
abstract：:In yeast, the STB5 gene encodes a transcriptional factor belonging to binuclear cluster class (Zn2Cys6) of transcriptional regulators specific to ascomycetes. In this study, we prepared the Kluyveromyces lactis stb5Δ strain and assessed its responses to different stresses. We showed that KlSTB5 gene is able to complem...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-019-00682-7
更新日期：2019-07-01 00:00:00
abstract：:This review discusses methods for the single-chain antibody fragment ($cFv) generation and scFv expression systems, and describes potential applications of scFv in the therapy of viral diseases and cancer, with emphasis on intracellularly expressed scFvs (intrabodies), application of scFvs in detection and diagnostics...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02993480
更新日期：2003-01-01 00:00:00
abstract：:Inhibition of methanogenesis in ruminal cultures was attempted by hindering thiamine availability through its degradation by 'polyphenols' and competition for active sites on enzymes and transporters using thiamine structural analogs. Effects on fermentation were small and not consistently reversed by adding thiamine....
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-009-0075-9
更新日期：2009-11-01 00:00:00
abstract：:A new strain of Bacillus sp. was isolated from a hot water spring in India. This strain generated a high activity of extracellular beta-galactosidase at 37 degrees C in shake flasks. The beta-galactosidase activity was found to increase continuously but the production rate was slower than with some other organisms rep...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02903706
更新日期：1999-01-01 00:00:00
abstract：:Treatment of Helicobacter pylori cells with several chaotropic agents resulted in different degrees of inhibition in the binding of the bacteria to hemin and Congo-red dye. Polyanions also yielded a >50% inhibitory effect. Furthermore, hydrophobic interaction chromatography was used to determine the relative surface h...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0043-z
更新日期：2011-05-01 00:00:00
abstract：:The biological activity and the presence of genes sfp and ituD (surfactin and iturin A) among Bacillus strains isolated from the Amazon basin were determined. Bacillus spp. were tested for hemolytic activity and inhibition of fungal growth by agar plate assays in parallel with PCR for identification of sfp and ituD ge...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0056-7
更新日期：2011-07-01 00:00:00
abstract：:The bacterial strain KDF8 capable of growth in the presence of diclofenac and codeine analgesics was obtained after chemical mutagenesis of nature isolates from polluted soils. The strain KDF8 was identified as Raoultella sp. based on its morphology, biochemical properties, and 16S rRNA gene sequence. It was deposited...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-017-0563-2
更新日期：2018-05-01 00:00:00
abstract：:Antibacterial effect of chitooligosaccharides (COS) and low molar mass chitosans (LMWC) is considered as one of the most important characteristics of chitosan (CS) hydrolysates. Here, we show the in vitro effect of different COS, LMWC, and CS on representative anaerobic bacteria isolated from human colon as a possibil...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-012-0138-1
更新日期：2012-07-01 00:00:00
abstract：:Inducible pigmentation changes were observed in pigmented strains of Brevibacterium sp. M27 and B. flavum treated with N-methyl-N'-nitro-N-nitrosoguanidine. The highest frequency of induction was reached already at a survival of 30-40% with the maximal yield of 6-10%. As compared with the initial yellow colour, three ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02928007
更新日期：1988-01-01 00:00:00
abstract：:Infiltration of immune cells into CNS is one of the essential events in multiple sclerosis (MS) development. Adhesion molecules like the intercellular adhesion molecule 1 (ICAM-1) play critical role in this process. Therefore, the ICAM1 gene containing two important single-nucleotide polymorphisms (SNPs) belongs to ca...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-017-0499-6
更新日期：2017-07-01 00:00:00
abstract：:When studying the kinetics of DNA synthesis, growth and cell division in Escherichia coli B/r after irradiation with different doses of UV-radiation (254 nm) we could demonstrate, by means of pulse incorporation of 3H-thymidine, a lag in DNA synthesis after the irradiation. The relative rate of the restored DNA synthe...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876446
更新日期：1982-01-01 00:00:00
abstract：:In this study, a method combining Raman spectroscopy with chemometric analysis was developed for detection of phage presence in raw milk and discrimination of Streptococcus thermophilus and Lactobacillus bulgaricus phages which are among the main phages causing problems in dairy industry. For this purpose, S. thermoph...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-018-0604-5
更新日期：2018-09-01 00:00:00
abstract：:The efficacy of the agents was evaluated on the basis of minimum inhibitory concentration (MIC) and time-kill curve analysis in Pseudomonas aeruginosa, Staphylococcus aureus, Acinetobacter baumannii, Mycobacterium smegmatis, and Citrobacter braakii; MIC was found to be 0.5, 4.0, 0.015625, 0.0078125, and 0.0625 mg/L in...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-009-0054-1
更新日期：2009-09-01 00:00:00
abstract：:The antifungal effect of 2-alkylthio-6-amino- and 2-alkylthio-6-formamidobenzothiazoles (22 derivatives in all) on Aspergillus niger and various Candida yeasts was tested. No significant effect was observed with A. niger. With the pathogenic yeast species C. albicans and C. guilliermondii, the most efficient derivativ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814461
更新日期：1989-01-01 00:00:00
abstract：:The present study was designed to characterize six Trueperella (T.) abortisuis strains, cultured over a period of 5 months from fetus and abortion material of six pigs of a single farm in Mecklenburg-West Pomerania federal state, Germany. It was of interest to investigate the epidemiological relationships of the six s...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-019-00753-9
更新日期：2020-06-01 00:00:00
abstract：:A total of 368 rotavirus RNA-positive (PAGE) stool samples collected continually during 1992-95 from infants and young children under five years of age hospitalized with acute gastroenteritis were serotyped using an enzyme immunoassay with VP7-specific monoclonal antibodies (ELISA with MAbs) for serotypes G1-G4. The s...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02817421
更新日期：2000-01-01 00:00:00
abstract：:Antibody response to major porin proteins of S. Typhi (OmpC and OmpF) was evaluated in sera of typhoid patients (culture positive, n = 28; Widal positive, n = 16). Sera from fever patients (n = 6) having etiology other than Salmonella, and normal healthy human controls (n = 18) were also included. No significant diffe...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-010-0086-6
更新日期：2010-09-01 00:00:00
abstract：:Competitive PCR method was developed for the detection and enumeration of Butyrivibrio fibrisolvens. Sequences of 16S rDNA were obtained from our isolates (serving as a source of data for primer design) and were distinguished into nine different groups of butyrivibria. Specific primers for two distinct groups were des...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02825888
更新日期：2001-01-01 00:00:00
abstract：:Physiological and biochemical properties were tested in 45 isolates of Aspergillus egyptiacus (16 isolates), Emericella nidulans (16) and Aspergillus versicolor (13). The three fungal species exhibited common and similar features. The big similarity between A. egyptiacus and E. nidulans was greater than between A. egy...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814449
更新日期：1994-01-01 00:00:00
abstract：:Inhibition activity of the alkaloid (-)-corypalmine on spore germination of plant pathogenic and saprophytic fungi (Alternaria solani, A brassicicola, A. brassicae, A. melongenae, Curvularia pallescens, C. lunata, C. maculans, Curvularisa sp., Colletotrichum sp., Helminthosporium speciferum, H. frumentacei, H. pennise...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02817654
更新日期：2002-01-01 00:00:00
abstract：:The regulation of gene transcription allows yeast cells to respond properly to changing environmental conditions. Several protein complexes take part in this process. They involve RNA polymerase complexes, chromatin remodeling complexes, mediators, general transcription factors and specific transcriptional regulators....
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/s12223-008-0044-8
更新日期：2008-01-01 00:00:00
