Stb5p is involved in Kluyveromyces lactis response to 4-nitroquinoline-N-oxide stress.

Abstract:

:In yeast, the STB5 gene encodes a transcriptional factor belonging to binuclear cluster class (Zn2Cys6) of transcriptional regulators specific to ascomycetes. In this study, we prepared the Kluyveromyces lactis stb5Δ strain and assessed its responses to different stresses. We showed that KlSTB5 gene is able to complement the deficiencies of Saccharomyces cerevisiae stb5Δ mutant. The results of phenotypic analysis suggested that KlSTB5 gene deletion did not sensitize K. lactis cells to oxidative stress inducing compounds but led to Klstb5Δ resistance to 4-nitroquinoline-N-oxide and hygromycin B. Expression analysis indicated that the loss of KlSTB5 gene function induced the transcription of drug efflux pump encoding genes that might contribute to increased 4-nitroquinoline-N-oxide and hygromycin B tolerance. Our results show that KlStb5p functions as negative regulator of some ABC transporter genes in K. lactis.

journal_title

Folia microbiologica

authors

Bencova A,Konecna A,Toth Hervay N,Gbelska Y

doi

10.1007/s12223-019-00682-7

subject

Has Abstract

pub_date

2019-07-01 00:00:00

pages

579-586

issue

4

eissn

0015-5632

issn

1874-9356

pii

10.1007/s12223-019-00682-7

journal_volume

64

pub_type

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