Abstract：
:Bacteria accumulate high amounts of potassium in the cytoplasm. For studying transport of K+ (with 86Rb as a marker) in bacteria (Staphylococcus aureus 17810S), the cells were depleted of the internal K+ pool by a DNP treatment. Kinetics and energetics of 86Rb transport was assayed with glucose as an exogenous energy source. It was shown that 86Rb uptake proceeded via a low affinity K+ transport system with an apparent K(m) of 2.3 mmol/L Rb+. Studies with the lipophilic cation TPP+ (tetraphenylphosphonium), the protonophore CCCP (carbonyl cyanide 3-chlorophenylhydrazone) and inhibitors (HQNO--2-heptyl-4-hydroxyquinoline N-oxide; iodoacetate) indicated that 86Rb transport was driven by delta psi (membrane potential) generated via the respiratory chain. The effect of Cd2+ on 86Rb transport was assayed with two energy donors--glucose and L-lactate. It was found that Cd2+ strongly inhibited delta psi-dependent 86Rb transport energized by cadmium-sensitive glucose oxidation, but was not toxic when cadmium-insensitive L-lactate was used as an energy source. The mechanism of these differential, substrate-dependent effects of Cd2+ on 86Rb transport is discussed.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Tynecka Z,Malm A,Kosikowska U,Kot Adoi
10.1007/BF02816378keywords:
subject
Has Abstractpub_date
1998-01-01 00:00:00pages
617-22issue
6eissn
0015-5632issn
1874-9356journal_volume
43pub_type
杂志文章abstract：:Skin microbiome main cultivable aerobes in human are coagulase-negative staphylococci and lipophilic corynebacteria. Staphylococcus strains (155) belonging to 10 species and 105 strains of Corynebacterium belonging to nine species from the skin swabs of healthy male volunteers were investigated to determine their enzy...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-014-0326-2
更新日期：2014-11-01 00:00:00
abstract：:Euglena ignobilis cells in natural puddle water of pH 7.8, when kept at 21 +/- 2 degrees C and under continuous light (intensity of approximately 30 micromol m(-2) s(-1)) in a culture chamber, decreased their speed of movement from > or = 78000 microm/min (after a 12-h cultivation), to 850-1300 microm/min after 18 h. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931412
更新日期：2005-01-01 00:00:00
abstract：:The characterization of 11- and 18-residue peptaibols (peptides synthesized by peptide synthetases) at Trichoderma harzianum CECT 2413 (a filamentous fungus) was performed. Using a heterologous probe from tex1, the only peptaibol synthetase cloned and characterized so far in Trichoderma species, was cloned; a region t...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02932165
更新日期：2006-01-01 00:00:00
abstract：:Fourteen of 200 Lactobacillus isolates from African fermented foods, viz. 'wara', 'kenkey', 'ugba', 'ogi', 'kunuzarki', 'fufu' and 'iru' were found to produce bacteriocins against L. plantarum and only three bacteriocinogenic isolates inhibited some of the food pathogens. Plasmid analysis of the 14 bacteriocin-produci...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814645
更新日期：1994-01-01 00:00:00
abstract：:Altered cell signaling is the molecular basis for cell proliferation occurring in association with several gamma herpesvirus infections. Three gamma herpesviruses, namely EBV/HHV-4, KSHV/HHV-8 and the MHV-68 (and/or MHV-72) and their unusual cell-pirated gene products are discussed in this respect. The EBV, KSHV as we...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02931360
更新日期：2003-01-01 00:00:00
abstract：:Raoultella spp. representatives are Gram-negative capsulated, nonmotile rods. These bacteria are found in the natural environment: plants, water, soil and insects. R. ornithinolytica is one of the three species of Raoultella. R. ornithinolytica is the only species within the genus which has the ability to produce orni...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-015-0390-2
更新日期：2015-11-01 00:00:00
abstract：:Fibrobacter succinogenes is a major cellulolytic species in the rumen. On the basis of molecular data, this species can be classified into four phylogenetic groups. Recently gathered ecological and physiological data have revealed the importance of this species, particularly phylogenetic group 1, in rumen fiber digest...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/s12223-008-0024-z
更新日期：2008-01-01 00:00:00
abstract：:A naturally deficient thiamine and methionine requiring strain of Bacillus coagulans (Ms 5) accumulates lysine in medium only when exogenous pyridoxine (optimal concentration, 0.1 mu g/ml) are supplied. Threonine exerts an inhibitory effect at higher concentrations but pyridoxine does not. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02877085
更新日期：1975-01-01 00:00:00
abstract：:The effects of pH on denitrifying enzyme activity (DEA) and on the ratio of the denitrification products, N(2)O and N(2), were determined in three pasture soils differing in cattle impact. The linkage between intrinsic differences in the denitrifying communities and pH effects on relative N(2)O production was also ass...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0045-x
更新日期：2011-05-01 00:00:00
abstract：:Manganese peroxidase (MnP) was produced by shallow stationary cultures of Phanerochaete chrysosporium growing on N-limited medium. Decolorization of sulfonphthalein (SP) dyes by MnP was investigated. The MnP activity profile and decolorization of SP dyes was correlated and almost all dyes were decolorized at pH 4.0. T...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931512
更新日期：2003-01-01 00:00:00
abstract：:A group of sixteen esculin-positive fluorescent pseudomonads isolated from an underground brook flowing through a cave complex was characterized by biotyping, multiple enzyme restriction fragment length polymorphism analysis of 16S rDNA (MERFLP), ribotyping and whole-cell fatty-acid methyl-esters analysis (FAME). All ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931556
更新日期：2004-01-01 00:00:00
abstract：:The synergistic anticandidal activity of three diketopiperazines [cyclo-(L-Pro-L-Leu) (1), cyclo-(D-Pro-L-Leu) (2), and cyclo-(D-Pro-L-Tyr) (3)] purified from a Bacillus sp. N strain associated with entomopathogenic nematode Rhabditis (Oscheius) in combination with amphotericin B and clotrimazole was investigated usin...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-013-0234-x
更新日期：2013-11-01 00:00:00
abstract：:Killer strains of Saccharomyces cerevisiae producing killer toxin K1 kill sensitive cells but are resistant to their own toxin. It is assumed that in the producer, an effective interaction between the external toxin and its plasma membrane receptor or the final effector is not possible on the grounds of a conformation...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02932122
更新日期：2006-01-01 00:00:00
abstract：:The microbial fuel cells (MFCs) are recognized to be highly effective for the biodegradation of phenol. For isolating the phenol-degrading bacteria, the sample containing 500 mg/L phenol was collected from the MFCs. The strain (WL027) was identified basing on the 16S rRNA gene analysis and phylogenetic analysis as Bac...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-017-0536-5
更新日期：2018-01-01 00:00:00
abstract：:Biodegradation of two amphiphilic "soft" antimicrobially active derivatives of lauric (dodecanoic) acid, a quaternary ammonium salt and an amine oxide bearing an amide or ester group, was followed using microorganisms from activated sludge. Primary biodegradation was determined by ion-selective electrodes, total biode...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814548
更新日期：1993-01-01 00:00:00
abstract：:Antibody response to major porin proteins of S. Typhi (OmpC and OmpF) was evaluated in sera of typhoid patients (culture positive, n = 28; Widal positive, n = 16). Sera from fever patients (n = 6) having etiology other than Salmonella, and normal healthy human controls (n = 18) were also included. No significant diffe...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-010-0086-6
更新日期：2010-09-01 00:00:00
abstract：:Ultrastructural changes induced by Rickettsia slovaca standard type (ST) and wild type (WT) were examined during their life cycle in L929 and Vero cells. R. slovaca invaded the cytoplasm of the host cell by phagocytosis on the 1st d p.i. Rickettsiae adhering to the cytoplasmic membrane were engulfed by cellular extens...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-009-0019-4
更新日期：2009-01-01 00:00:00
abstract：:It is proposed that the transcription of the c-2 repressor gene initiates at two promoters, pre and prm, similarly to the transcription of the c-I gene of lambda phage. In the P22 phage, these promoters are defined by mutations of c54 and K5. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876780
更新日期：1975-01-01 00:00:00
abstract：:During cultivation in a minimal medium with glucose Alternaria tenuissima and Aspergillus vesicolor produce constitutively alpha- and beta-glucanases. Fractions of beta-1,3-glucanases exhibiting affinity for laminarin were separated by means of gel filtration chromatography. Two neutral beta-1,3-glucanases with affini...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876394
更新日期：1980-01-01 00:00:00
abstract：:The level of an antibiotic capable of inhibiting the etiological agent at the site of infection is an essential prerequisite for successful antibiotic therapy. In some cases, locally applied antibiotics may compensate for limitations of systemic administration and shorten systemic therapy. We aimed at verifying to wha...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0093-2
更新日期：2012-09-01 00:00:00
abstract：:Effects of increasing doses of UV-B radiation on photosynthetic efficiency (PE) and motility in Chlamydomonas reinhardtii were investigated. The exposure time ranged from 10 to 120 min at 0.73 W/m2 UV-B radiation. A decrease in compensation points (CP) was followed by an increase in PE on extending the UV-B treatment ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02817448
更新日期：2000-01-01 00:00:00
abstract：:The majority of antibiotics and substances with diverse biological activity used in medicine are produced by actinomycetes, nonfilamentous bacteria and fungi. Other microorganisms, such as myxobacteria, pseudomonads, nocardias, basidiomycetes, marine microorganisms, enterobacteria, halobacteria, hyperthermophiles etc....
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02993460
更新日期：2003-01-01 00:00:00
abstract：:Based on the ability to attach to polymeric surfaces, the formation of biofilms was determined in 5 wild-type strains (Pseudomonas aeruginosa, Klebsiella pneumoniae, Acinetobacter baumanii, Escherichia coli, Staphylococcus warneri). Using modified Christensen method, minimum regrowth concentration (MRC) of piperacilli...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931650
更新日期：2004-01-01 00:00:00
abstract：:Biochemical properties of a termostable alkaline phosphatase obtained from the mycelium extract of A. caespitosus were described. The enzyme was purified 42-fold with 32% recovery by DEAE-cellulose and concanavalin A-Sepharose chromatography. The molar mass estimated by Sephacryl S-200 or by 7% SDS-PAGE was 138 kDa an...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931303
更新日期：2007-01-01 00:00:00
abstract：:The root nodules of Phaseolus mungo (a herbaceous leguminous pulse) contained a high amount of 3-indolylacetic acid (IAA). A tryptophan pool present in the nodule might play the role of precursor for IAA production. From the root nodule a Rhizobium sp. was isolated. The symbiont produced a large amount of IAA (142 mic...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-008-0054-6
更新日期：2008-01-01 00:00:00
abstract：:A wild-type strain of Candida albicans (S1, ATCC 10261) was used to obtain stable auxotrophic colony morphological mutants (mutant M5 producing only true hyphae and mutant M2 containing 90 % blastospores and 10 % pseudohyphae) by induced mutagenesis. A hybrid was produced by somatic hybridization between these 2 mutan...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931528
更新日期：2004-01-01 00:00:00
abstract：:Biotechnological conversion of low-cost agro-industrial by-products, such as industrial waste or terpenes from the distillation of essential oils from plants into more valuable oxygenated derivatives, can be achieved by using microbial cells or enzymes. In Argentina, the essential oil industry produces several tons of...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-015-0422-y
更新日期：2016-03-01 00:00:00
abstract：:The microflora of the chernozem soil mineralized 62% of the lactose retained on a column consisting of three 10-g layers of the soil at a daily flow of 48 mg of the sugar. Only 45% of the sugar was mineralized when the daily flow was 136 mg. The highest value of the beta-galactosidase activity in the system of heteroc...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02927259
更新日期：1981-01-01 00:00:00
abstract：:Several amino acids affected the amount of bacitracin produced by Bacillus licheniformis ATCC 10716 and the high-yielding mutant B. licheniformis AL. The possibility of an amino acid control mechanism for bacitracin biosynthesis is discussed. The two strains showed a different response to the addition of some amino ac...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02926454
更新日期：1979-01-01 00:00:00
abstract：:Sixty four biopsy specimens of oral Candida leukoplakias were examined. Histological sections were stained with periodic acid-Schiff (PAS) reagent and by Grocott's silver method. C. albicans isolates were cultivated from all the patients but fungal hyphae were proved histologically only in 23 of them. PAS-positive and...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814437
更新日期：2001-01-01 00:00:00
