Characterization of esculin-positive Pseudomonas fluorescens strains isolated from an underground brook.

Abstract:

:A group of sixteen esculin-positive fluorescent pseudomonads isolated from an underground brook flowing through a cave complex was characterized by biotyping, multiple enzyme restriction fragment length polymorphism analysis of 16S rDNA (MERFLP), ribotyping and whole-cell fatty-acid methyl-esters analysis (FAME). All strains were phenotypically close to Pseudomonas fluorescens, but they revealed high biochemical variability as well as some reactions atypical for P. fluorescens species. Because identification of pseudomonads by of biochemical testing is often unclear, further techniques were employed. Fingerprints obtained by MERFLP clearly showed that all strains represent P. fluorescens species. Ribotyping separated the strains analyzed into four groups corresponding almost completely (with the exception of one strain) to the clustering based on biochemical profiles. FAME analysis grouped all the strains into one cluster together with the P. putida (biotype A, B), P. chlororaphis and P. fluorescens biotype F representatives, but differentiated them from other FAME profiles of all pseudomonads included in the standard library TSBA 40 provided by MIDI, Inc.

journal_title

Folia microbiologica

authors

Svec P,Stegnerová H,Durnová E,Sedlácek I

doi

10.1007/BF02931556

keywords:

subject

Has Abstract

pub_date

2004-01-01 00:00:00

pages

725-30

issue

6

eissn

0015-5632

issn

1874-9356

journal_volume

49

pub_type

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