Abstract:
:This is the first report describing precipitation of bacteriophage particles with zinc chloride as a method of choice to isolate infectious lytic bacteriophages against Pectobacterium spp. and Dickeya spp. from environmental samples. The isolated bacteriophages are ready to use to study various (ecological) aspects of bacteria-bacteriophage interactions. The method comprises the well-known precipitation of phages from aqueous extracts of the test material by addition of ZnCl2, resuscitation of bacteriophage particles in Ringer's buffer to remove the ZnCl2 excess and a soft agar overlay assay with the host bacterium to isolate infectious individual phage plaques. The method requires neither an enrichment step nor other steps (e. g., PEG precipitation, ultrafiltration, or ultracentrifugation) commonly used in other procedures and results in isolation of active viable bacteriophage particles.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Czajkowski R,Ozymko Z,Lojkowska Edoi
10.1007/s12223-015-0411-1subject
Has Abstractpub_date
2016-01-01 00:00:00pages
29-33issue
1eissn
0015-5632issn
1874-9356pii
10.1007/s12223-015-0411-1journal_volume
61pub_type
杂志文章abstract::The green alga Scenedesmus intermedius was grown in synchronous culture under manganese or calcium deficiency for six successive generations. The growth rate, pigment and protein contents gradually decreased in comparison with the control. In Mn-deficient cells, the rate of oxygen evolution was sharply decreased. This...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02817561
更新日期:2000-01-01 00:00:00
abstract::Altered cell signaling is the molecular basis for cell proliferation occurring in association with several gamma herpesvirus infections. Three gamma herpesviruses, namely EBV/HHV-4, KSHV/HHV-8 and the MHV-68 (and/or MHV-72) and their unusual cell-pirated gene products are discussed in this respect. The EBV, KSHV as we...
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/BF02931360
更新日期:2003-01-01 00:00:00
abstract::The ability of six bifidobacterial strains (3 of human origin and 3 isolates from fermented milk products) to utilize glucose, lactose, melezitose, sucrose, raffinose, and stachyose was determined. Dairy-related bifidobacterial strains were identified as Bifidobacterium animalis (2 strains) or as B. pseudolongum (1 st...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02818784
更新日期:2002-01-01 00:00:00
abstract::Many serious diseases caused by Staphylococcus aureus appear to be associated with biofilms. Therefore, we investigated the biofilm-forming ability of the methicillin-resistant S. aureus (MRSA) isolates collected from hospitalized patients. As many as 96 % strains had the ability to form biofilm in vitro. The majority...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-012-0175-9
更新日期:2013-01-01 00:00:00
abstract::Levels of phenotypic and genotypic diversity among seven Megasphaera elsdenii strains recovered from rumen contents of cattle, sheep and lambs were determined by a combination of antibiotic-resistance analysis and PCR fingerprint techniques targeted both to the ribosomal RNA operon (ARDRA, RISA) and the whole genome (...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931818
更新日期:2006-01-01 00:00:00
abstract::Sporulation was repressed in the parent strain by various carbon sources whereas glucose-resistant mutants were resistant to them but not to glycerol 2-phosphate. Both mycobacillin and dipicolinic acid synthesis were repressed in the parent by some of the compounds tested, viz. glucose, pyruvate and glycerol 2-phospha...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02928000
更新日期:1986-01-01 00:00:00
abstract::Inhibition of methanogenesis in ruminal cultures was attempted by hindering thiamine availability through its degradation by 'polyphenols' and competition for active sites on enzymes and transporters using thiamine structural analogs. Effects on fermentation were small and not consistently reversed by adding thiamine....
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-009-0075-9
更新日期:2009-11-01 00:00:00
abstract::Some Bacillus subtilis mutants with different levels of homoserine dehydrogenase were described. Strains that do not accumulate methionine have a high homoserine dehydrogenase activity. Low activity was detected in mutants where cell growth was completely inhibited by 0.7 mmol/L methionine. A low concentration of dime...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02884064
更新日期:1991-01-01 00:00:00
abstract::Mycobacterium microti-infected mouse peritoneal macrophages produced high amounts of prostaglandin E2 (PGE2) and nitric oxide (NO) when activated with interferon-gamma (IFN-gamma). In order to understand the relation between PGE2 and NO production and the expression of interleukin-12 (IL-12), interleukin-10 (IL-10) an...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02818541
更新日期:2001-01-01 00:00:00
abstract::Excessive use of antibiotics contributes to the selection of resistant bacteria and intestinal colonization with multiresistant pathogens poses a risk factor for subsequent infections. The present study assessed vancomycin-resistant enterococci (VRE) carriage rates in patients admitted to our tertiary care hospital. S...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-019-00751-x
更新日期:2020-06-01 00:00:00
abstract::The level of an antibiotic capable of inhibiting the etiological agent at the site of infection is an essential prerequisite for successful antibiotic therapy. In some cases, locally applied antibiotics may compensate for limitations of systemic administration and shorten systemic therapy. We aimed at verifying to wha...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-011-0093-2
更新日期:2012-09-01 00:00:00
abstract::A new simple method used to eliminate polysaccharides that cause problems during DNA isolation was established for 6 different white-rot fungi using 1% hexadecyltrimethylammonium bromide (CTAB) as wash buffer and followed by centrifugation. Variation in the DNA yield and quality was ascertained using precipitating age...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931383
更新日期:2004-01-01 00:00:00
abstract::The properties of the 37400 oncogenic strain of Agrobacterium tumefaciens are described. This strain was derived from the VI lysogenic strain originally isolated by Hamilton from a Zinnia elegans tumour. Strain 37400 has a number of properties which render it suitable for quantitative and genetic studies. It is cured ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876963
更新日期:1976-01-01 00:00:00
abstract::Two classes of newly synthesized amphiphilic compounds, phenolic antioxidants ("phenolics") and N-oxides exert in vivo antioxidant effects on live S. cerevisiae cells. Both groups have low toxicity, phenolics being more toxic than N-oxides and compounds with a longer alkyl chain having higher toxicity than those with ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02932187
更新日期:2007-01-01 00:00:00
abstract::Structural and functional properties of prokaryotic DNA methyltransferases are summarized. The different aspects of the role of DNA methylation which influences DNA-protein interaction in restriction and modification of DNA and in mismatch repair, DNA replication and gene expression are discussed. ...
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/BF02815658
更新日期:1992-01-01 00:00:00
abstract::The effect of immersion time and cell concentration in the attachment of several lactic acid bacteria with antibacterial activity to beef-muscle surface was studied. The number of firmly attached bacteria increased with immersion time in the case of Pediococcus acidilacti, Lactobacillus sake, Lactococcus cremoris (two...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814710
更新日期:1996-01-01 00:00:00
abstract::During cultivation in a minimal medium with glucose Alternaria tenuissima and Aspergillus vesicolor produce constitutively alpha- and beta-glucanases. Fractions of beta-1,3-glucanases exhibiting affinity for laminarin were separated by means of gel filtration chromatography. Two neutral beta-1,3-glucanases with affini...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876394
更新日期:1980-01-01 00:00:00
abstract::In ostriches and pheasants, there is still limited information relating to staphylococci and their properties. Biogenic amines (BAs) are nitrogenous low-molecular-weight substances with biological functions in animals, plants and microorganisms. In this study, we focused on BA production by targeted faecal staphylococ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-017-0492-0
更新日期:2017-05-01 00:00:00
abstract::The color of light (white, red, blue, and green) had a significant effect on the growth and reproductive processes (both in the nucleocytoplasmic and chloroplast compartment of the cells) in synchronous cultures of Scenedesmus obliquus. This effect decreased in the order red > white > blue > green. In the same order, ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931828
更新日期:2006-01-01 00:00:00
abstract::Vancomycin is often used in orthopedic surgery as a local prophylaxis of bacterial infection. The aim of this work was to compare the release of vancomycin and its biologically inactive crystalline degradation products (CDP-1) during in vitro experiments from different types of local antibiotic delivery systems (bone ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-019-00752-w
更新日期:2020-06-01 00:00:00
abstract::In Slovakia, dairy products made from ewes' milk have a long tradition. These products include the lactic acid product called "žinčica" which is a by-product occurring during the preparation of ewes' lump cheese. There is no information in the literature regarding the special properties of the microbiota, especially l...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-019-00703-5
更新日期:2020-02-01 00:00:00
abstract::Oxidizing and assimilating ability of the yeast Candida utilis 49 was tested with 21 different low-boiling organic compounds which come as components of raw synthetic ethanol. The highest yields of yeast dry weight were obtained with ethanol (72.0%), propanol (48.2%), ethyl acetate (43.4%) and acetic acid (34.2%). To ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876965
更新日期:1976-01-01 00:00:00
abstract::The potentiality of twenty-five fungal species belonging to 14 genera isolated from Egyptian soils to produce mevinolin, a hypocholesterolemic agent, when grown on selected substrates was tested. For the first screening samples of culture filtrates were tested by TLC and the positive results were further estimated by ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02826557
更新日期:1997-01-01 00:00:00
abstract::Activity of purified alanylaminopeptidase of Pseudomonas sp. measured in the presence of the alanine derivative of 2-naphthoic acid (NA-Ala) is inhibited by 1,10-phenanthroline, EDTA, bestatin and amastatin; this finding supports the conclusion that this enzyme is a metallo-aminopeptidase. A decrease of its activity i...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02817643
更新日期:2002-01-01 00:00:00
abstract::The soil microbial population, determined over a period of 19 months and the elemental chemical composition measured by X-ray fluorescence techniques were followed in two uncultivated salt-affected areas of Greece, namely Lantza and Klidi. The ecological conditions at Lantza remained stable during the period of study ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02877387
更新日期:1983-01-01 00:00:00
abstract::The assumption that the pyruvate decarboxylase activity of Saccharomyces carlsbergensis is the main limiting factor determining the formation rate and the total amount of d(--)-l-hydroxy-l-phenyl-propanone (phenylacetylcarbinol, PAC) produced was not confirmed. An increase of about 30% of the total amount of the PAC p...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02877396
更新日期:1982-01-01 00:00:00
abstract::Using an in vitro cytotoxicity assay (51Cr-release), it was shown that bilirubin exerts a cytotoxic effect on both adult and newborn human lymphocytes after a 1-d incubation of cells with bilirubin. The effect of bilirubin on human granulocytes was less pronounced; the association of the cytotoxic effect with a functi...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02923520
更新日期:1985-01-01 00:00:00
abstract::Asymptomatic uropathogenic Escherichia coli (UPECs) are the leading cause of asymptomatic bacteriuria (ABU) in humans. So this study aimed to identify and characterize ABU UPECs from hospitalized patients of Kolkata, India, with respect to their antibiogram profile, phylogeny, pathogenicity islands, and virulence fact...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-019-00685-4
更新日期:2019-07-01 00:00:00
abstract::Diseases of the central nervous system (CNS) mean for the human organism a potentially dangerous situation. An investigation of cerebrospinal fluid (CSF) provides important information about a character of CNS impairment in the decision-making diagnostic and therapeutic algorithm. The authors present a brief overview ...
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/s12223-018-0663-7
更新日期:2019-05-01 00:00:00
abstract::The newly described type strains Arcanobacterium pinnipediorum DSM 28752T and Arcanobacterium wilhelmae DSM 102162T, initially isolated from an anal swab of a harbor seal (Sammra et al. Int J Syst Evol Microbiol 65:4539-4543, 2015) and the genital tract of a rhinoceros (Sammra et al. Int J Syst Evol Microbiol 67:2093-...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-018-0610-7
更新日期:2018-11-01 00:00:00