Application of zinc chloride precipitation method for rapid isolation and concentration of infectious Pectobacterium spp. and Dickeya spp. lytic bacteriophages from surface water and plant and soil extracts.

Abstract:

:This is the first report describing precipitation of bacteriophage particles with zinc chloride as a method of choice to isolate infectious lytic bacteriophages against Pectobacterium spp. and Dickeya spp. from environmental samples. The isolated bacteriophages are ready to use to study various (ecological) aspects of bacteria-bacteriophage interactions. The method comprises the well-known precipitation of phages from aqueous extracts of the test material by addition of ZnCl2, resuscitation of bacteriophage particles in Ringer's buffer to remove the ZnCl2 excess and a soft agar overlay assay with the host bacterium to isolate infectious individual phage plaques. The method requires neither an enrichment step nor other steps (e. g., PEG precipitation, ultrafiltration, or ultracentrifugation) commonly used in other procedures and results in isolation of active viable bacteriophage particles.

journal_title

Folia microbiologica

authors

Czajkowski R,Ozymko Z,Lojkowska E

doi

10.1007/s12223-015-0411-1

subject

Has Abstract

pub_date

2016-01-01 00:00:00

pages

29-33

issue

1

eissn

0015-5632

issn

1874-9356

pii

10.1007/s12223-015-0411-1

journal_volume

61

pub_type

杂志文章
  • Effect of manganese and calcium deficiency on the growth and oxygen exchange of Scenedesmus intermedius cultured for successive generations.

    abstract::The green alga Scenedesmus intermedius was grown in synchronous culture under manganese or calcium deficiency for six successive generations. The growth rate, pigment and protein contents gradually decreased in comparison with the control. In Mn-deficient cells, the rate of oxygen evolution was sharply decreased. This...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02817561

    authors: Adam MS,Issa AA

    更新日期:2000-01-01 00:00:00

  • Gamma herpesviruses: pathogenesis of infection and cell signaling.

    abstract::Altered cell signaling is the molecular basis for cell proliferation occurring in association with several gamma herpesvirus infections. Three gamma herpesviruses, namely EBV/HHV-4, KSHV/HHV-8 and the MHV-68 (and/or MHV-72) and their unusual cell-pirated gene products are discussed in this respect. The EBV, KSHV as we...

    journal_title:Folia microbiologica

    pub_type: 杂志文章,评审

    doi:10.1007/BF02931360

    authors: Rajcáni J,Kúdelová M

    更新日期:2003-01-01 00:00:00

  • Specific growth rate of bifidobacteria cultured on different sugars.

    abstract::The ability of six bifidobacterial strains (3 of human origin and 3 isolates from fermented milk products) to utilize glucose, lactose, melezitose, sucrose, raffinose, and stachyose was determined. Dairy-related bifidobacterial strains were identified as Bifidobacterium animalis (2 strains) or as B. pseudolongum (1 st...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02818784

    authors: Rada V,Bartonová J,Vlková E

    更新日期:2002-01-01 00:00:00

  • Biofilm density and detection of biofilm-producing genes in methicillin-resistant Staphylococcus aureus strains.

    abstract::Many serious diseases caused by Staphylococcus aureus appear to be associated with biofilms. Therefore, we investigated the biofilm-forming ability of the methicillin-resistant S. aureus (MRSA) isolates collected from hospitalized patients. As many as 96 % strains had the ability to form biofilm in vitro. The majority...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-012-0175-9

    authors: Szczuka E,Urbańska K,Pietryka M,Kaznowski A

    更新日期:2013-01-01 00:00:00

  • Limited genetic variability in Megasphaera elsdenii strains.

    abstract::Levels of phenotypic and genotypic diversity among seven Megasphaera elsdenii strains recovered from rumen contents of cattle, sheep and lambs were determined by a combination of antibiotic-resistance analysis and PCR fingerprint techniques targeted both to the ribosomal RNA operon (ARDRA, RISA) and the whole genome (...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02931818

    authors: Piknová M,Bíres O,Javorský P,Pristas P

    更新日期:2006-01-01 00:00:00

  • Relationship between sporulation and synthesis of mycobacillin and dipicolinic acid under condition of catabolite repression in Bacillus subtilis.

    abstract::Sporulation was repressed in the parent strain by various carbon sources whereas glucose-resistant mutants were resistant to them but not to glycerol 2-phosphate. Both mycobacillin and dipicolinic acid synthesis were repressed in the parent by some of the compounds tested, viz. glucose, pyruvate and glycerol 2-phospha...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02928000

    authors: Majumdar S,Basu S,Das SK,Bose SK

    更新日期:1986-01-01 00:00:00

  • The effects of thiamine inhibition on ruminal fermentation: a preliminary study.

    abstract::Inhibition of methanogenesis in ruminal cultures was attempted by hindering thiamine availability through its degradation by 'polyphenols' and competition for active sites on enzymes and transporters using thiamine structural analogs. Effects on fermentation were small and not consistently reversed by adding thiamine....

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-009-0075-9

    authors: Ungerfeld EM,Rust SR,Burnett R

    更新日期:2009-11-01 00:00:00

  • Effect of dimethyl sulfoxide on lysine production by a mutant of Bacillus subtilis with homoserine dehydrogenase activity.

    abstract::Some Bacillus subtilis mutants with different levels of homoserine dehydrogenase were described. Strains that do not accumulate methionine have a high homoserine dehydrogenase activity. Low activity was detected in mutants where cell growth was completely inhibited by 0.7 mmol/L methionine. A low concentration of dime...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02884064

    authors: Kalcheva HO,Shanskaya VO,Smutny J,Maluta SS

    更新日期:1991-01-01 00:00:00

  • Effects of prostaglandin E2 and nitric oxide inhibitors on the expression of interleukin-10, interleukin-12 and MHC class-II molecules in Mycobacterium microti-infected and interferon-gamma-treated mouse peritoneal macrophages.

    abstract::Mycobacterium microti-infected mouse peritoneal macrophages produced high amounts of prostaglandin E2 (PGE2) and nitric oxide (NO) when activated with interferon-gamma (IFN-gamma). In order to understand the relation between PGE2 and NO production and the expression of interleukin-12 (IL-12), interleukin-10 (IL-10) an...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02818541

    authors: Mittal J,Dogra N,Vohra H,Majumdar S

    更新日期:2001-01-01 00:00:00

  • Assessing the intestinal carriage rates of vancomycin-resistant enterococci (VRE) at a tertiary care hospital in Hungary.

    abstract::Excessive use of antibiotics contributes to the selection of resistant bacteria and intestinal colonization with multiresistant pathogens poses a risk factor for subsequent infections. The present study assessed vancomycin-resistant enterococci (VRE) carriage rates in patients admitted to our tertiary care hospital. S...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-019-00751-x

    authors: Franyó D,Kocsi B,Bukta EE,Szabó J,Dombrádi Z

    更新日期:2020-06-01 00:00:00

  • Bone grafts as vancomycin carriers in local therapy of resistant infections.

    abstract::The level of an antibiotic capable of inhibiting the etiological agent at the site of infection is an essential prerequisite for successful antibiotic therapy. In some cases, locally applied antibiotics may compensate for limitations of systemic administration and shorten systemic therapy. We aimed at verifying to wha...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-011-0093-2

    authors: Melicherčík P,Jahoda D,Nyč O,Klapková E,Barták V,Landor I,Pokorný D,Judl T,Sosna A

    更新日期:2012-09-01 00:00:00

  • Improving the yield and quality of DNA isolated from white-rot fungi.

    abstract::A new simple method used to eliminate polysaccharides that cause problems during DNA isolation was established for 6 different white-rot fungi using 1% hexadecyltrimethylammonium bromide (CTAB) as wash buffer and followed by centrifugation. Variation in the DNA yield and quality was ascertained using precipitating age...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02931383

    authors: Kuhad RC,Kapoor RK,Lal R

    更新日期:2004-01-01 00:00:00

  • Properties of the cured oncogenic strain 37400 of Agrobacterium tumefaciens.

    abstract::The properties of the 37400 oncogenic strain of Agrobacterium tumefaciens are described. This strain was derived from the VI lysogenic strain originally isolated by Hamilton from a Zinnia elegans tumour. Strain 37400 has a number of properties which render it suitable for quantitative and genetic studies. It is cured ...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02876963

    authors: Bezdĕk M,Reich J,Tkadlecek L

    更新日期:1976-01-01 00:00:00

  • Cell-protective and antioxidant activity of two groups of synthetic amphiphilic compounds--phenolics and amine N-oxides.

    abstract::Two classes of newly synthesized amphiphilic compounds, phenolic antioxidants ("phenolics") and N-oxides exert in vivo antioxidant effects on live S. cerevisiae cells. Both groups have low toxicity, phenolics being more toxic than N-oxides and compounds with a longer alkyl chain having higher toxicity than those with ...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02932187

    authors: Krasowska A,Sigler K

    更新日期:2007-01-01 00:00:00

  • Biological function of DNA methylation.

    abstract::Structural and functional properties of prokaryotic DNA methyltransferases are summarized. The different aspects of the role of DNA methylation which influences DNA-protein interaction in restriction and modification of DNA and in mismatch repair, DNA replication and gene expression are discussed. ...

    journal_title:Folia microbiologica

    pub_type: 杂志文章,评审

    doi:10.1007/BF02815658

    authors: Hubácek J

    更新日期:1992-01-01 00:00:00

  • Attachment of lactic acid bacteria to beef-muscle surfaces.

    abstract::The effect of immersion time and cell concentration in the attachment of several lactic acid bacteria with antibacterial activity to beef-muscle surface was studied. The number of firmly attached bacteria increased with immersion time in the case of Pediococcus acidilacti, Lactobacillus sake, Lactococcus cremoris (two...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02814710

    authors: Benito Y,Pin C,Fernández MF,Marín ML,Selgas MD,García ML,Casas C

    更新日期:1996-01-01 00:00:00

  • The extracellular system of beta-1,3-glucanases of Alternaria tenuissima and Aspergillus vesicolor.

    abstract::During cultivation in a minimal medium with glucose Alternaria tenuissima and Aspergillus vesicolor produce constitutively alpha- and beta-glucanases. Fractions of beta-1,3-glucanases exhibiting affinity for laminarin were separated by means of gel filtration chromatography. Two neutral beta-1,3-glucanases with affini...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02876394

    authors: Jirků V,Kraxnerová B,Krumphanzl V

    更新日期:1980-01-01 00:00:00

  • Effect of lantibiotic gallidermin against biogenic amine-producing faecal staphylococci from ostriches and pheasants.

    abstract::In ostriches and pheasants, there is still limited information relating to staphylococci and their properties. Biogenic amines (BAs) are nitrogenous low-molecular-weight substances with biological functions in animals, plants and microorganisms. In this study, we focused on BA production by targeted faecal staphylococ...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-017-0492-0

    authors: Lauková A,Kandričáková A,Pleva P,Buňková L,Ščerbová J

    更新日期:2017-05-01 00:00:00

  • The effect of light color on the nucleocytoplasmic and chloroplast cycle of the green chlorococcal alga Scenedesmus obliquus.

    abstract::The color of light (white, red, blue, and green) had a significant effect on the growth and reproductive processes (both in the nucleocytoplasmic and chloroplast compartment of the cells) in synchronous cultures of Scenedesmus obliquus. This effect decreased in the order red > white > blue > green. In the same order, ...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02931828

    authors: Cepák V,Pribyl P,Vítová M

    更新日期:2006-01-01 00:00:00

  • Vancomycin and its crystalline degradation products released from bone grafts and different types of bone cement.

    abstract::Vancomycin is often used in orthopedic surgery as a local prophylaxis of bacterial infection. The aim of this work was to compare the release of vancomycin and its biologically inactive crystalline degradation products (CDP-1) during in vitro experiments from different types of local antibiotic delivery systems (bone ...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-019-00752-w

    authors: Klapkova E,Nescakova M,Melichercik P,Jahoda D,Dunovska K,Cepova J,Prusa R

    更新日期:2020-06-01 00:00:00

  • Some safety aspects of enterococci isolated from Slovak lactic acid dairy product "žinčica".

    abstract::In Slovakia, dairy products made from ewes' milk have a long tradition. These products include the lactic acid product called "žinčica" which is a by-product occurring during the preparation of ewes' lump cheese. There is no information in the literature regarding the special properties of the microbiota, especially l...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-019-00703-5

    authors: Lauková A,Kandričáková A,Bino E,Tomáška M,Kološta M,Kmeť V,Strompfová V

    更新日期:2020-02-01 00:00:00

  • Assimilation spectrum of the yeast Candida utilis 49 used for producing fodder yeast from synthetic ethanol.

    abstract::Oxidizing and assimilating ability of the yeast Candida utilis 49 was tested with 21 different low-boiling organic compounds which come as components of raw synthetic ethanol. The highest yields of yeast dry weight were obtained with ethanol (72.0%), propanol (48.2%), ethyl acetate (43.4%) and acetic acid (34.2%). To ...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02876965

    authors: Sestáková M

    更新日期:1976-01-01 00:00:00

  • Mevinolin production by some fungi.

    abstract::The potentiality of twenty-five fungal species belonging to 14 genera isolated from Egyptian soils to produce mevinolin, a hypocholesterolemic agent, when grown on selected substrates was tested. For the first screening samples of culture filtrates were tested by TLC and the positive results were further estimated by ...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02826557

    authors: Shindia AA

    更新日期:1997-01-01 00:00:00

  • Regulation of the activity of intracellular alanylaminopeptidase synthesized by Pseudomonas sp.

    abstract::Activity of purified alanylaminopeptidase of Pseudomonas sp. measured in the presence of the alanine derivative of 2-naphthoic acid (NA-Ala) is inhibited by 1,10-phenanthroline, EDTA, bestatin and amastatin; this finding supports the conclusion that this enzyme is a metallo-aminopeptidase. A decrease of its activity i...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02817643

    authors: Jankiewicz U,Bielawski W

    更新日期:2002-01-01 00:00:00

  • Interaction between microorganisms, chemical composition and environment in salt-affected soils.

    abstract::The soil microbial population, determined over a period of 19 months and the elemental chemical composition measured by X-ray fluorescence techniques were followed in two uncultivated salt-affected areas of Greece, namely Lantza and Klidi. The ecological conditions at Lantza remained stable during the period of study ...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02877387

    authors: Douka CE,Xenoulis AC,Paradellis T

    更新日期:1983-01-01 00:00:00

  • Effect of pyruvate decarboxylase activity and of pyruvate concentration on the production of 1-hydroxy-1-phenylpropanone in Saccharomyces carlsbergensis.

    abstract::The assumption that the pyruvate decarboxylase activity of Saccharomyces carlsbergensis is the main limiting factor determining the formation rate and the total amount of d(--)-l-hydroxy-l-phenyl-propanone (phenylacetylcarbinol, PAC) produced was not confirmed. An increase of about 30% of the total amount of the PAC p...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02877396

    authors: Vojtísek V,Netrval J

    更新日期:1982-01-01 00:00:00

  • The in vitro cytotoxic effect of bilirubin on human lymphocytes and granulocytes.

    abstract::Using an in vitro cytotoxicity assay (51Cr-release), it was shown that bilirubin exerts a cytotoxic effect on both adult and newborn human lymphocytes after a 1-d incubation of cells with bilirubin. The effect of bilirubin on human granulocytes was less pronounced; the association of the cytotoxic effect with a functi...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/BF02923520

    authors: Miler I,Indrová M,Bubeník J,Vondrácek J

    更新日期:1985-01-01 00:00:00

  • Incidence of multidrug resistance, pathogenicity island markers, and pathoadaptive FimH mutations in uropathogenic Escherichia coli isolated from asymptomatic hospitalized patients.

    abstract::Asymptomatic uropathogenic Escherichia coli (UPECs) are the leading cause of asymptomatic bacteriuria (ABU) in humans. So this study aimed to identify and characterize ABU UPECs from hospitalized patients of Kolkata, India, with respect to their antibiogram profile, phylogeny, pathogenicity islands, and virulence fact...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-019-00685-4

    authors: Ghosh A,Mukherjee M

    更新日期:2019-07-01 00:00:00

  • Recommendations for cerebrospinal fluid analysis.

    abstract::Diseases of the central nervous system (CNS) mean for the human organism a potentially dangerous situation. An investigation of cerebrospinal fluid (CSF) provides important information about a character of CNS impairment in the decision-making diagnostic and therapeutic algorithm. The authors present a brief overview ...

    journal_title:Folia microbiologica

    pub_type: 杂志文章,评审

    doi:10.1007/s12223-018-0663-7

    authors: Hepnar D,Adam P,Žáková H,Krušina M,Kalvach P,Kasík J,Karpowicz I,Nasler J,Bechyně K,Fiala T,Mamiňák M,Malíková I,Mareš J,Šigut V,Tokár Z,Vránová J

    更新日期:2019-05-01 00:00:00

  • Further characteristics of Arcanobacterium pinnipediorum DSM 28752T and Arcanobacterium wilhelmae DSM 102162T, two novel species of genus Arcanobacterium.

    abstract::The newly described type strains Arcanobacterium pinnipediorum DSM 28752T and Arcanobacterium wilhelmae DSM 102162T, initially isolated from an anal swab of a harbor seal (Sammra et al. Int J Syst Evol Microbiol 65:4539-4543, 2015) and the genital tract of a rhinoceros (Sammra et al. Int J Syst Evol Microbiol 67:2093-...

    journal_title:Folia microbiologica

    pub_type: 杂志文章

    doi:10.1007/s12223-018-0610-7

    authors: Sammra O,Rau J,Wickhorst J,Alssahen M,Hassan AA,Lämmler C,Prenger-Berninghoff E,Abdulmawjood A

    更新日期:2018-11-01 00:00:00