Abstract：
:Glycerol-based medium (BM) with and without the addition of 1 g/L ascorbic acid (Asc) and/or 100 mg/L (+/-)-catechin (Cat) was tested for the storage of three commercial wine yeasts at -20 degrees C. The medium supplemented with Asc was also used to store 706 strains to verify the maintenance of the liquid state. A decline in survival throughout the storage period was observed. The media containing Asc maintained viability better than the other three. The BM caused a loss of viability of 7 orders for one strain and of 6 orders for the other two. All three strains exhibited a loss of viability of 4 orders when stored in BM+Asc. Two strains decreased viability by 5 orders while one strain by 4 orders, when stored in BM+Cat. Two strains decreased viability by 6 orders while one strain by 5 orders, when stored in BM+Asc+Cat. Regarding the physical state of the medium tested on 706 yeast strains, three cases were observed: completely liquid (56.5 %), liquid with only the upper part frozen (40.4 %) without involving the yeast biomass settled at the bottom, and completely frozen (3.12 %). It is practicable to prepare a BM that remains liquid at -20 degrees C enhancing yeast viability when Asc is added as cryoprotectant.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Sidari R,Caridi Adoi
10.1007/s12223-009-0036-3subject
Has Abstractpub_date
2009-01-01 00:00:00pages
230-2issue
3eissn
0015-5632issn
1874-9356journal_volume
54pub_type
杂志文章abstract：:The green alga Scenedesmus intermedius was grown in synchronous culture under manganese or calcium deficiency for six successive generations. The growth rate, pigment and protein contents gradually decreased in comparison with the control. In Mn-deficient cells, the rate of oxygen evolution was sharply decreased. This...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02817561
更新日期：2000-01-01 00:00:00
abstract：:Single chain Fv (scFv) antibodies (generated by phage display technology, molecules representing new and efficient tools in the research and diagnostics of infectious diseases) against the capsid protein (p25) of Maedi-Visna virus were selected. Several clones of p25 specific scFv antibodies were identified; one of th...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931380
更新日期：2003-01-01 00:00:00
abstract：:Phytoplankton successions under ice cover (January-March) were determined in four oligotrophic lakes (Burtjärn, Aspen, Vialamptjärn and Storkorstjärn) located in North-Eastern Sweden. The total phosphorus concentration in the lakes was less than 10 micrograms/L. Lake Burtjärn (reference lake) had a similar hydrology a...
journal_title：Folia microbiologica
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doi：10.1007/BF02931370
更新日期：2003-01-01 00:00:00
abstract：:The possibility of utilizing derivatives of 4-methylumbelliferone (4-MUBF) for the determination of activity of enzymes from the group of hydrolyses was tested in 20 strains of 12 species of mycobacteria. The method proved to be suitable especially for determination of arylsulphatase, beta-D-galactose and acid phospha...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876698
更新日期：1980-01-01 00:00:00
abstract：:Structural and functional properties of prokaryotic DNA methyltransferases are summarized. The different aspects of the role of DNA methylation which influences DNA-protein interaction in restriction and modification of DNA and in mismatch repair, DNA replication and gene expression are discussed. ...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02815658
更新日期：1992-01-01 00:00:00
abstract：:Virulence factors were compared in 15 Aeromonas spp. isolated from faeces of patients with Aeromonas-associated gastroenteritis and in 81 strains isolated from food. Strains from food did not show differences in the distribution of virulence factors when compared with strains isolated from faeces. However, 88.8% of Ae...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814323
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abstract：:Studies on the production of extracellular cellulase in two aquatic hyphomycetes Lunulospora curvula and Flagellospora penicillioides have shown that several factors such as carbon source, nitrogen source, pH and temperature affect the production of the enzyme. Experiments have shown that carboxymethyl cellulose is th...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814357
更新日期：1991-01-01 00:00:00
abstract：:The biological activity and the presence of genes sfp and ituD (surfactin and iturin A) among Bacillus strains isolated from the Amazon basin were determined. Bacillus spp. were tested for hemolytic activity and inhibition of fungal growth by agar plate assays in parallel with PCR for identification of sfp and ituD ge...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0056-7
更新日期：2011-07-01 00:00:00
abstract：:The microbial fuel cells (MFCs) are recognized to be highly effective for the biodegradation of phenol. For isolating the phenol-degrading bacteria, the sample containing 500 mg/L phenol was collected from the MFCs. The strain (WL027) was identified basing on the 16S rRNA gene analysis and phylogenetic analysis as Bac...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-017-0536-5
更新日期：2018-01-01 00:00:00
abstract：:Some Bacillus subtilis mutants with different levels of homoserine dehydrogenase were described. Strains that do not accumulate methionine have a high homoserine dehydrogenase activity. Low activity was detected in mutants where cell growth was completely inhibited by 0.7 mmol/L methionine. A low concentration of dime...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02884064
更新日期：1991-01-01 00:00:00
abstract：:The rosettes formed by mouse peritoneal macrophages or DCH-5 cells and TNP-erythrocytes coated with anti-TNP antibodies of different isotypes were inhibited to various extent by monosaccharides. The most effective inhibitors were N-acetylglucosamine, glucosamine, mannose and N-acetylneuraminic acid in 1-5 mmol/L conce...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814584
更新日期：1992-01-01 00:00:00
abstract：:In the present study, three Arcanobacterium pluranimalium strains isolated from bovine milk samples of three cows of three farms (two cows with subclinical mastitis) could successfully be identified by phenotypical investigations, by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-T...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-016-0450-2
更新日期：2016-09-01 00:00:00
abstract：:The effects of pH on denitrifying enzyme activity (DEA) and on the ratio of the denitrification products, N(2)O and N(2), were determined in three pasture soils differing in cattle impact. The linkage between intrinsic differences in the denitrifying communities and pH effects on relative N(2)O production was also ass...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0045-x
更新日期：2011-05-01 00:00:00
abstract：:Regulatory mechanism of primary metabolism responsible for the biosynthesis of peptide ergot alkaloids was proposed on the basis of experimental results from the production phase of Claviceps purpurea (FR.) Tul. in parasitic and saprophytic cultures. The production-phase metabolism is characterized by uncoupling of gl...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02927365
更新日期：1981-01-01 00:00:00
abstract：:Proteinase production by Lactobacillus murinus was influenced by temperature, glucose concentration, initial pH and nitrogen sources. Maximum proteinase production occurred at 45 degrees C, pH 6.6 and with 0.5% (W/V) glucose. Tryptone, peptone and gelatin inhibited it. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02877263
更新日期：1987-01-01 00:00:00
abstract：:The level of an antibiotic capable of inhibiting the etiological agent at the site of infection is an essential prerequisite for successful antibiotic therapy. In some cases, locally applied antibiotics may compensate for limitations of systemic administration and shorten systemic therapy. We aimed at verifying to wha...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0093-2
更新日期：2012-09-01 00:00:00
abstract：:The potentiality of twenty-five fungal species belonging to 14 genera isolated from Egyptian soils to produce mevinolin, a hypocholesterolemic agent, when grown on selected substrates was tested. For the first screening samples of culture filtrates were tested by TLC and the positive results were further estimated by ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02826557
更新日期：1997-01-01 00:00:00
abstract：:Isolation and structure elucidation was carried out of flavonoid constituents found in fractionated extracts of the seeds and green leaves of Daucus carota L. var. boissieri (Apiaceae). The flavonoids are mainly apigenin, luteolin, their glycosidic precursors and 2,4,5-trimethoxybenzaldehyde. Fatty acids, hydrocarbons...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02903720
更新日期：1999-01-01 00:00:00
abstract：:In the present study, 193 Aspergillus strains were isolated from a total of 100 soil samples of pistachio orchards, which all of them were identified as Aspergillus flavus as the most abundant species of Aspergillus section Flavi existing in the environment. Approximately 59%, 81%, and 61% of the isolates were capable...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0090-5
更新日期：2012-01-01 00:00:00
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journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876611
更新日期：1980-01-01 00:00:00
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journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-009-0019-4
更新日期：2009-01-01 00:00:00
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journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-014-0339-x
更新日期：2014-11-01 00:00:00
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journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02903715
更新日期：1999-01-01 00:00:00
abstract：:Purification of streptococcal extracellular NAD+ nucleosidase is associated with changes of kinetic properties. A high-molecular weight component is required for a full activity of the enzyme. The component is not produced by bacteria and is present in the Todd-Hewitt cultivation medium, the beef-heart extract serving...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876396
更新日期：1980-01-01 00:00:00
abstract：:Stabilized sewage sludge is applied to agricultural fields and farmland due to its high organic matter content. The aim of this study was to investigate the effects of two types of sludge stabilization, mesophilic anaerobic digestion (MAD) and thermophilic anaerobic digestion (TAD), on bacterial communities in sludge,...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-015-0396-9
更新日期：2015-11-01 00:00:00
abstract：:Blastoschizomyces capitatus is an uncommon, opportunistic pathogenic fungus, which causes invasive and disseminated infections. This microorganism is normally present in both environmental and normal human flora. Within a host, B. capitatus is able to grow in both unicellular yeast and multicellular filamentous growth...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-013-0273-3
更新日期：2014-03-01 00:00:00
abstract：:The effect of dental plaque bacteria on LFA-1 beta expression on peripheral blood leukocytes was studied in 20 patients with early-onset periodontitis and in 10 healthy controls. Stimulation of PMN with selected dental plaque bacteria which play a role in the pathogeny of periodontitis significantly increased the expr...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02815697
更新日期：1996-01-01 00:00:00
abstract：:A small-colony forming variant of Escherichia coli with a mutation in the ncf gene was analysed. The alternation of the protein composition in the cytoplasmic membrane and the interaction with K and E group colicins indicated a membrane mutation. The effect of this mutation on some membrane-bound processes, the activi...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02885601
更新日期：1977-01-01 00:00:00
abstract：:Five trisubstituted quinazolones and eight trisubstituted quinazoline-4-thiones have been tested for antibacterial effects by a microdilution method. Four derivatives exerted a significant effect on E. coli, P. aeruginosa, S. aureus and B. subtilis (IC50 < 100 mg/L). In the bacterium P. aeruginosa six quinazolines sho...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02817411
更新日期：2000-01-01 00:00:00
abstract：:A naturally deficient thiamine and methionine requiring strain of Bacillus coagulans (Ms 5) accumulates lysine in medium only when exogenous pyridoxine (optimal concentration, 0.1 mu g/ml) are supplied. Threonine exerts an inhibitory effect at higher concentrations but pyridoxine does not. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02877085
更新日期：1975-01-01 00:00:00
